Antihemolytic Effect Research Articles

Anti-Staphylococcal Activities of Rosmarinus officinalis and Myrtus communis Essential Oils through ROS-Mediated Oxidative Stress

Rosmarinus officinalis and Myrtus communis essential oils (EOs) are well-known for their ethno-pharmaceutical properties. In the present study, we have analyzed the chemical composition of both EOs by gas chromatography-mass spectrometry. Then we assessed their antibacterial, antibiofilm, and anti-virulence actions against the opportunistic pathogen Staphylococcus aureus. The cytotoxic effect of agents tested against this bacterium was investigated by monitoring reactive oxygen-species (ROS) generation and antioxidant-enzyme (catalase) production. Regarding the antistaphylococcal effects, our results showed antibacterial efficacy of both Eos and their combination, where the minimum inhibitory concentrations ranged between 0.7 and 11.25 mg/mL. A combination of tested agents showed the highest anti-hemolytic and anti-protease effects. Additionally, association between EOs displayed more potency against the development of biofilm performed by S. aureus, with percentage of removal reaching 74%. The inhibitory impacts of EOs on S. aureus virulence factors were discovered to be concentration-dependent. Furthermore, our results provide insight on the abilities of R. officinalis and M. communis EOs, as well as their potential in combination, to generate ROS and affect oxidative stress enzyme catalase in S. aureus, leading to their antagonistic effect against this pathogen.

Antimicrobial Activity of Quercetin, Naringenin and Catechin: Flavonoids Inhibit Staphylococcus aureus-Induced Hemolysis and Modify Membranes of Bacteria and Erythrocytes.

Search for novel antimicrobial agents, including plant-derived flavonoids, and evaluation of the mechanisms of their antibacterial activities are pivotal objectives. The goal of this study was to compare the antihemolytic activity of flavonoids, quercetin, naringenin and catechin against sheep erythrocyte lysis induced by α-hemolysin (αHL) produced by the Staphylococcus aureus strain NCTC 5655. We also sought to investigate the membrane-modifying action of the flavonoids. Lipophilic quercetin, but not naringenin or catechin, effectively inhibited the hemolytic activity of αHL at concentrations (IC50 = 65 ± 5 µM) below minimal inhibitory concentration values for S. aureus growth. Quercetin increased the registered bacterial cell diameter, enhanced the fluidity of the inner and surface regions of bacterial cell membranes and raised the rigidity of the hydrophobic region and the fluidity of the surface region of erythrocyte membranes. Our findings provide evidence that the antibacterial activities of the flavonoids resulted from a disorder in the structural organization of bacterial cell membranes, and the antihemolytic effect of quercetin was related to the effect of the flavonoid on the organization of the erythrocyte membrane, which, in turn, increases the resistance of the target cells (erythrocytes) to αHL and inhibits αHL-induced osmotic hemolysis due to prevention of toxin incorporation into the target membrane. We confirmed that cell membrane disorder could be one of the direct modes of antibacterial action of the flavonoids.

THE EFFECT OF AUXILIARY DRUGS ON PHOTODYNAMIC CYTOLYSIS IN PHOTODYNAMIC THERAPY

The effect of pyridine-containing and phenolic compounds (Mexidol, Emoxypine, Vitamin В6 , Paracetamol, Propofol, Coenzyme Q10) and imidazole-containing Dexdor on photoinduced hemolysis in PDT was studied in an experiment (on erythrocytes of healthy people’s blood). It was found that Mexidol and Emoxypine showed themselves not as antioxidants, but as powerful agents to enhance the hemolytic effect, while in the area of small doses they showed a membraneprotective antihemolytic effect. Therefore, the possibility of their use as concomitant drugs in PDT is problematic and additional clinical studies are required. The anesthetic Dexdor (dexmedetomidine) did not have a noticeable effect on the rate of hemolysis, which makes it possible to recommend it as a priority drug for drug support of PDT. Sedative Propofol (2,4-diisopropylphenol) and Paracetamol (p-acetoaminophenol) as most phenolic compounds caused significant inhibition of hemolysis, which makes their use in PDT problematic. Coenzyme Q10 (its reduced form – ubiquinol) showed a pronounced inhibitory effect on the hemolysis process, however, it can be recommended as an effective membrane-protective drug to protect the membranes of healthy cells

The role of quercetin in the formation of titanium dioxide nanoparticles for nanomedical applications

The current work aimed to synthesize and characterize titanium dioxide nanoparticles (TiO2NPs) using quercetin (QE) and evaluate their biological activities, i.e., anti-hemolytic, anti-inflammatory, and cytotoxicity effects. The crystallographic phase and morphology of biosynthesized QE-TiO2NPs were characterized by XRD (X-Ray Diffraction) and TEM/FE-SEM (Transmission/Field-Emission Scanning Electron Microscopy) micrographs. Functional groups involved in the synthesis process were determined by FTIR spectroscopy (Fourier Transform-Infrared Spectroscopy). Based on the characterization results, selected QE-TiO2NPs showed a rutile phase, spherical shape, and a size range of 7.3–39 nm. The QE-TiO2NPs did not show a hemolytic effect. They indicated 95.3% red blood cells (RBCs) membrane stabilization activity and 82.6% inhibition of bovine serum albumin (BSA) denaturation, similar to a standard drug, which proved their anti-inflammatory effects. The attained results from cytotoxicity studies revealed the toxic effects of QE-TiO2NPs with IC50 values below 100 and 50 μg/mL for human breast cancer cells of MCF-7 and melanoma cancer cells of A375, respectively. These NPs did not significantly affect normal skin fibroblast cells up to 50 μg/mL and only showed a 16% inhibition rate on the cell viability at 100 μg/mL. These NPs also induced excessive ROS generation. This work established the blood/biocompatibility and excellent nanomedical applications of biosynthesized QE-TiO2NPs.

Evaluation of thein vivoAntioxidant and Antihemolytic Potential ofPeganum harmalaMethanolic Seed Extract

Background:Peganum harmala L. is a medicinal herbal plant widely used in traditional medicine in various countries, especially in Algeria. This study aimed to carry out the extraction of P. harmala seeds and to evaluate the antioxidant and antihemolytic potentials of 100 mg/kg methanol extract in plasma, blood, and liver of Swiss albino mice.Methods:The antioxidant capacity of the plasma samples was performed using free radical scavenging (DPPH assay) and reducing power activities. However, the antihemolytic effect of methanol extract was measured in the blood induced by tBH. The biomarkers of oxidative stress were evaluated in liver tissues by measuring the activity of catalase enzyme (CAT), and the level of GSH and MDA products.Results:The results showed that 100 mg/kg of MOHE did not show any significant changes in plasma antioxidant capacity by DPPH assay when we compared with a control group (32.70 ± 4,45 %), however, the reducing power capacity of plasma is remarkable in methanolic extract and VitC groups. Fifty percent of red blood cell lysis was calculated (HT50) for measuring the antihemolytic effect, the results showed that MOHEhad a protective effect against RBC hemolysis with HT50 = 78,51 ± 11,32 min. In addition, MOHE increases the activity of CAT, GSH levels whereas it decreases the MDA levels in the homogenate liver, which demonstrates that MOHE inhibits the lipid peroxidation in the liver.Conclusion:This plant has a strong pharmacological power due to its secondary metabolite. However, its uses for therapeutic purposes is not without danger and exposes the risk of intoxication.

국내 수유부로부터 수집한 모유의 항산화 및 항용혈 효과

To confirm the antioxidant and anti-hemolytic activity in human breast milk (HBM), evaluated DPPH radical scavenging and anti-hemolytic activities against hemolytic strains. As a result of measuring the pH of HBM according to the days after childbirth, the pH of the HBM less than 10 days after childbirth was 6.63±0.26, the longer the lactation period, the lower the pH of breast milk. As a result of measuring the pH of HBM according to the days after childbirth, the pH of HBM less than 10 days after childbirth was 6.63±0.26 and over 30 days after childbirth was 6.45±0.15. These results indicated that the pH of HBM decreases as the days after childbirth increases. In contrast DPPH radical scavenging activity was the highest in HBM of over 30 days after childbirth (63.72±12.24%), that is presumed to be due to the fatty acid content in HBM. As a result of confirming hemolysis of HBM, weak hemolysis was observed in some HBM samples, that are caused to be hemolysis by bacteria produced the hemolysin in HBM. Therefore, result of analyzing the anti-hemolytic effect of HBM against the hemolytic bacteria, the effect of breast milk against the gram-positive S. aureus CCARM 3855 was 66.37±21.36%, gram-negative P. aeruginosa CCARM 0223 was 29.74±9.31%. In addition, HBM samples of the less than 10 days after childbirth showed highest each anti-hemolytic activity against hemolytic strain. Thus, this study can be suggested that it will be basic study for preventing hemolytic disease in newborns.

Antithrombotic and antihemolytic effects of Lagerstroemia speciosa (Lythraceae) aqueous extract

Introduction: Aqueous extract of Lagerstroemia speciosa (EALS) (Lythraceae) is widely used to treat diabetes. This plant has been shown an in vitro thrombolytic activity that indicates its potential to prevent the formation of blood clots in vivo. Thus, this study was undertaken to evaluate the antithrombotic and antihemolytic effects of EALS. Methods: Rats of both sexes (200 ± 5 g) were divided into five groups of six animals. Each group received orally distilled water, EALS (250, 500, 1000 mg/kg), and acetylsalicylic acid (100 mg/kg) for five days. After treatment, the FeCl3-induced arterial thrombus formation method was used to determine occlusion time. A coagulometer was used to detect activated partial thromboplastin time (aPTT) and prothrombin time (PT). Rabbit blood was used to determine clot lysis activity in vitro and antihemolytic activity using the 2,2-azobis hydrochloride (2-methylpropionamidine) (AAPH) method. Results: EALS increased the occlusion time in a dose-dependent manner. At the dose of 1000 mg/kg, EALS increased the occlusion time significantly, from 4.59 ± 2.45 minutes to 15.52 ± 2.38 minutes (P<0.01). At high concentrations (1-4 mg/mL), EALS showed a significant increase in aPPT and PT (P<0.05). Streptokinase and EALS (4 mg/mL) induced significant clot lysis with percentage values of 78.48 ± 2.2 % and 49.5 ± 1.53 %, respectively (P<0.001). EALS inhibited AAPH-induced hemolysis. Conclusion: EALS exhibited antithrombotic and antihemolytic activities. The antithrombotic property of the plant could be attributed to its anticoagulant and thrombolytic activities. Regular consumption of L. speciosa leaves may prevent or treat thrombotic diseases.

The Fluorescence Detection of Phenolic Compounds in Plicosepalus curviflorus Extract Using Biosynthesized ZnO Nanoparticles and Their Biomedical Potential.

A facile, eco-friendly fluorescence approach based on the biogenic formation of zinc oxide nanoparticles using the biomass of Plicosepalus curviflorus shoots was developed. The suggested approach was employed to analyze three phenolic compounds (catechin, curviflorside, and curviflorin) isolated from the shoots of P. curviflorus. The surface morphology of the prepared ZnONPs was characterized by carrying out different microscopic and spectroscopic investigations. A significant UV-Vis absorption peak of ZnONPs was recognized at 345 nm and the FT-IR spectra of the isolated catechin, curviflorside, and curviflorin in the presence of sodium dodecyl sulfate (SDS) and ZnONPs were recorded at λem 470, 490, and 484 nm after excitation at λex 380, 420, and 410 nm. The suggested fluorescence method displayed linear concentration ranges of 10–120, 5–100, and 10–150 μg mL−1 for the three isolated compounds, respectively. The shoot extract, isolated compounds, and ZnONPs were screened for antibacterial and anticancer effects against four different types of bacterial strains and HeLa cells, respectively. The ZnONPs exhibited the highest zone of inhibition against Escherichia coli and Staphylococcus aureus strains when compared with pure, isolated compounds and shoot extract. The anticancer potential of ZnONPs (64%) was stronger as compared to the 160 µg mL−1 of shoot extract (49%), catechin (52%), curviflorside (54%), and curviflorin (58%) at 160 µg mL−1. Moreover, all the samples were investigated for hemolysis activity and showed a potent anti-hemolytic effect. The developed analytical method showed excellent sensitivity and reliability for the concurrent analysis of the isolated bioactive markers.

Anti-Hemolytic and Antimicrobial Effects against Multidrug-Resistant Bacteria of Enterococcus faecalis Isolated from Human Breast Milk

Anti-Hemolytic and Antimicrobial Effects against Multidrug-Resistant Bacteria of <i>Enterococcus faecalis</i> Isolated from Human Breast Milk

Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants.

Herbal and traditional medicines can play a pivotal role in combating cancer and neglected tropical diseases. Ajuga bracteosa, family Lamiaceae, is an important medicinal plant. The genetic transformation of A. bracteosa with rol genes of Agrobacterium rhizogenes further enhances its metabolic content. This study aimed at undertaking the molecular, phytochemical, and in vitro biological analysis of A. bracteosa extracts. We transformed the A. bracteosa plant with rol genes and raised the regenerants from the hairy roots. Transgenic integration and expression of rolB were confirmed by conventional polymerase chain reaction (PCR) and qPCR analysis. The methanol: chloroform crude extracts of wild-type plants and transgenic regenerants were screened for in vitro antibacterial, antihemolytic, cytotoxic, anticancer, and leishmanial activity. Among all plants, transgenic line 3 (ABRL3) showed the highest expression of the rolB gene. Fourier transform infra-red (FTIR) analysis confirmed the enhanced number of functional groups of active compounds in all transgenic lines. Moreover, ABRL3 exhibited the highest antibacterial activity, minimum hemolytic activity (CC50 = 7293.05 ± 7 μg/mL) and maximum antileishmanial activity (IC50 of 56.16 ± 2 μg/mL). ABRL1 demonstrated the most prominent brine shrimp cytotoxicity (LD5039.6 ± 4 μg/mL). ABRL3 was most effective against various human cancer cell lines with an IC50 of 57.1 ± 2.2 μg/mL, 46.2 ± 1.1 μg/mL, 72.4 ± 1.3 μg/mL, 73.3 ± 2.1 μg/mL, 98.7 ± 1.6 μg/mL, and 97.1 ± 2.5 μg/mL against HepG2, LM3, A549, HT29, MCF-7, and MDA-MB-231, respectively. Overall, these transgenic extracts may offer a cheaper therapeutic source than the more expensive synthetic drugs.

Edessy–Tawfik-Covid 19 Supplements (CovETS).

Medicinal plants like thyme, Anise, and Rhus Coriaria contains many essential elements which possesses antiviral, antioxidant properties 5. It plays an important role in prophylaxis and cure of viral infection. It is generally recognized that it affects the lipid layer in the virus envelope, disrupting the adsorption to the host cell and preventing the virus from penetrating the host cell, positively contributing to the infection 6. One of the main disruptive effects of the SARS-CoV2 virus is the destruction of hemoglobin and erythrocyte and the resulting decrease in the capacity to carry blood oxygen and carbon dioxide 7. The study investigated the anti hemolytic effect of tannins which have a modifying effect on the erythrocyte membrane structure. It was determined that tannins were incorporated into the erythrocyte membrane, and increased the hardness of the hydrophilic region of the lipid bilayer of the RBCs 8. A study isolated 6-Pentadecylsalicylic acid from medicinal plants...

Protective Effect of Various Extracts of Allium hirtifolium and Satureja khuzestanica Plants on AAPH-Induced Oxidative Hemolysis

Background: Allium hirtifolium and Satureja khuzestanica are the Iranian endemic plants and proper candidates for antioxidant studies. This study investigated the antioxidant, anti-hemolytic properties, and phytochemical composition in different extracts of A. hirtifolium and S. khuzestanica. Methods: Hydroalcoholic, methanolic, and n-hexane extracts of A. hirtifolium and S. khuzestanica plants were prepared using soaking and ultrasonic methods. Different plant extracts were evaluated for the presence of secondary metabolites using standard methods based on colorimetric analysis. The antioxidant properties of the compounds were measured by the ferric reducing ability of power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. Finally, the anti-hemolytic effects of the extracts were investigated using the 2,2’-Azobis(2-amidinopropane) dihydrochloride (AAPH) methods. Results: Phytochemical analysis indicated the presence of flavonoids, phenolic acids, glycosides, steroids, and terpenoids in S. khuzestanica, as well as amino acid compounds, flavonoids, saponins, glycosides, and phenols in A. hirtifolium. FRAP assay showed that the methanolic extract of S. khuzestanica and the n-hexane extract of A. hirtifolium have the highest total antioxidant activity. The results of the DPPH assay also demonstrated that the minimum IC50 was related to the hydroalcoholic extract of S. khuzestanica (18.58 µg/mL) and the n-hexane extract of A. hirtifolium (87.95 µg/mL). In general, the extracts of both plants could reduce the percentage of AAPH-induced hemolysis while being significant only in some concentrations (P&lt;0.001). Conclusion: Overall, A. hirtifolium and S. khuzestanica can be used as a herbal supplement in the human diet due to their anti-hemolytic effects.

Antimicrobial Activity and Protective Effect of Tuscan Bee Pollens on Oxidative and Endoplasmic Reticulum Stress in Different Cell-Based Models.

Bee pollen is an apiary product of great interest owing to its high nutritional and therapeutic properties. This study aimed to assess the cellular antioxidant activity and the antihemolytic effect of Castanea, Rubus, and Cistus bee pollens on human erythrocytes. We also tested the antimicrobial potential of each sample on selected Gram-negative and Gram-positive bacteria. Finally, the effect of Castanea bee pollen, showing the best phytochemical profile, was analyzed on human microvascular endothelial cells exposed to thapsigargin, used as endoplasmic reticulum (ER) stressor. Our results showed good biological activities of all bee pollen samples that, under oxidative conditions, significantly improved the erythrocytes’ antioxidant activity and limited cell lyses. Castanea and Cistus showed comparable antihemolytic activities, with higher % hemolysis inhibition than Rubus. All samples exerted antimicrobial activity with different selectivity among all the tested microorganisms with minimal inhibitory concentration values ranging from 5 to 10 mg/mL. Finally, Castanea bee pollen was effective in reducing gene over-expression and oxidation process arising from thapsigargin treatment, with a maximum protective effect at 10 µg/mL. In conclusion, bee pollen represents a potential natural antibacterial and a good nutraceutical product useful in the prevention of free radical and ER stress-associated diseases.

In vitro investigation of antioxidant and antihemolytic activities of three Lamiaceae species from Morocco

BackgroundLkhzama (Lavandula officinalis), Mard-doch (Origanum majorana), and Lahbak (Ocimum basilicum) are aromatic and medicinal plants widely used in Moroccan folk medicine as a treatment for numerous diseases including liver diseases, rheumatism, and diabetes. This study was undertaken to examine the antioxidant and antihemolytic activities of the aqueous extracts of these plants. The antioxidant activity was evaluated using three in vitro tests: DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity, FRAP (ferric reducing antioxidant power assay), and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. The antihemolytic activity of plant extracts was evaluated against AAPH (2,2′-azobis(2-amidino-propane) dihydrochloride)-induced erythrocyte hemolysis.ResultsOur findings showed that all plant extracts displayed significant antioxidant and antihemolytic effects. In fact, among the studied plant extracts, the highest antioxidant power was recorded in Origanum majorana, based on DPPH (IC50 = 12.29 μg/mL), ABTS (226.13 μmol TE/g DW), and FRAP (477.82 μmol TE/g DW) assays. Moreover, the same plant also showed the best membrane protective effect (269.55%). Whereas, Ocimum basilicum exhibited the lowest antioxidant activity using DPPH (IC50 = 42.85 μg/mL), ABTS (IC50 = 226.13 μmol TE/g DW), and FRAP (IC50 = 172.84 μmol TE/g DW) and, thus, the lowest membrane protective effect (182.70%).ConclusionThis result supports the use of these plants in folk medicine for preventing and treating many diseases, especially those related to oxidative stress.

In vitro Anti-hemolytic Effect, in vivo Anti-inflammatory and in vitro Anti-oxidant Activity of Anchusa azurea Mill.

Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria; it is locally named hamham. It is effective in the treatment of various diseases. The aim of the present study is to determine the antioxidant, anti-inflammatory, and anti- hemolytic effects of phenolic fractions from Anchusa azurea Mill. In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In-vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE, and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 μg/ml. Vitamin C is used as a standard. The free-radical attack was measured by measuring the HT50 (Half-Hemolysis Time). The anti-inflammatory effect using PMA on mice of the methanolic extract (CrE) was evaluated. The quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains a higher amount of polyphenols. However, chloroform extract (ChE) presents a higher amount of flavonoids. AcE showed an important scavenging activity using the DPPH radical (IC50= 68.35 μg/ml). The results showed that AcE also exhibited a significant inhibition effect on the oxidation of β-carotene/linoleic acid (84.33 %). All extracts increased the HT50 values (Half-Hemolysis Time) in a dose-dependent manner. The three highest concentrations (21.18, 42.37, and 84.74 μg / ml) of ChE caused a very significant delay (p ≤ 0.001) of hemolysis compared to the negative control and the positive control "VIT C". The anti-inflammatory effect of using PMA on mice showed that the methanolic extract (CrE) of AA reduced the weight of the ear edema. This plant has a strong pharmacological power, which supports its traditional medicinal use.

Valorisation of the Green Waste Parts from Large-Leaved Buttercup (Ranunculus macrophyllus Desf.): Phenolic Profile and Health Promoting Effects Study

Due to the extensive use of Ranunculus macrophyllus Desf. roots for medicinal purposes, most of the leafy green parts are just wasted. The aim of this work is to valorize the leafy green parts and promote their application in different modern industries. For this purpose, we studied the phenolic profile of R. macrophyllus Desf. (RM-B) using chromatographic and spectrophotometric methods and we tested the in-vitro antioxidant activity and the in-vivo effect of RM-B on plasma and liver antioxidant statuts. RM-B contained high amounts of polyphenols (675 mg GAE/100 g dry weigh dw) and flavonoids (105 mg QE/100 g dw). In-vitro, RM-B exhibited promising radical scavenging activity against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS+·) (IC50: 247 µg/mL), hydrogen peroxide radicals (IC50: 626 µg/mL) and inhibited oxidative red blood cells hemolysis (IC50: 120 µg/mL), RM-B also showed strong reducing power (982 µM FeSO4/mg extract). In-vivo, RM-B improved the radical scavenging ability and reducing power of plasma and enhanced liver antioxidant status by increasing catalase and reduced glutathione levels and decreasing malondialdhyde levels without altering the key serum biochemical parameters reflecting liver and kidney functions. Polyphenols identified using capillary LC-DAD and LC–MS/MS analyses like hesperidin (131.2 mg/100 g dw), rutin (29.0 mg/100 g dw) and p-coumaric acid (5.8 mg/100 g dw), may be responsible for the health promoting effects of RM-B. We may conclude that R. macrophyllus Desf. is a good source of beneficial polyphenols with strong antioxidant, anti-hemolytic and health-promoting effects, which promotes its use in pharmaceutical, medicinal and nutraceutical industries.

An Invitro Study on Predicted Glycemic Index and Bioactive Component of Fortified - Bread Using Senna Auriculata

Bread is an affordable staple food available world wide. People demand for the high quality breads for consumption.Bread is considered to be a daily diet food for most of the people around the world. Foods with reduced glycemic index shows less risk of some chronic diseases like diabets and stroke. Senna auriculata a plant compound which is a potential inhibitor of diabetes. Low concentration possessed high radical scavenging activity, 90% of radicals were scavenged at lower concentrations. It further proved to have the anti-hemolytic effect. The extract inhibits the alpha amylase and alpha glycosidase activity. The bread contains large amount of digestible starch which leads to glycemic index. The foods with higher glycemic index leads to type 2 diabetes mellitus. Incorporation of Senna auriculata in bread is the effective method to produce low glycemic index foods. This study was aimed to evaluate digestability of starch and glycemic index in bread through the incorporating Senna auriculata as fortified bread sample. At 60 mins of dialysate, the concentration of resistant starch of bread sample with Senna Auriculata exhibited to be low when compared with the control. The Senna auriculata fortified bread sample is compared with the control which the regular bread based on their crust, colour, symmetry, texure, eating quality, and overall quality of the bread. Bread with 6% of extract was sensory evaluated. Bioactive components like phenolic, flavonoid found to be retained in fortified bread sample. Based on the biofunctional properties developed the evaluation is analysed at the retention time of the process. The sample has higher resistant starch and lower glycemic index. This overall invitro findings gives us a promising result that due to the lower glycemic effect the Senna auriculata fortified bread sample is situable and recommended for diabetes patients.

The In vivo and in vitro antioxidant and anti-hemolytic effect of Algerian Centaurea calcitrapa L. extracts

In the present study, aerial part of Centaurea calcitrapa L. were extracted with solvent of varying polarity allowed their separation into three main subfractions, the analysis of methanol crud (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE contains the highest amount of flavonoids (50,71 ± 0,65 mg Eq / Quercetin g dry extract and 31,96 ± 0,39 mg Eq Rutin / g dry extract). Using DPPH assay, the highest activity was observed with EaE (IC50 = 0,037 ± 0,0006 mg / ml). The β-carotene / linoleic acid bleaching assay revealed that the extracts have a very important antioxidant activity. The results showed that CrE has the highest antioxidant activity. The antioxidant activity of the CrE is confirmed by an in vivo assay in mice, using two doses: CrD1 (50 mg/kg/day) and CrD2 (100 mg/kg/day) during 21 days. Total antioxidant capacity of plasma and red blood cells was measured. The half-life (HT50), which corresponds to 50% of cell lysis was calculated, the results showed that both groups treated with plant extract had a protective effect against erythrocytes hemolysis (CrD2: HT50= 167,3 ± 3,72 min). The CrD2 group showed a strong scavenging activity using DPPH assay (51,64 ± 5,24 %), higher than that of Vit C group (38,92 ± 1,72 %). All results confirmed that the extracts have a dose dependent effect on the growth of overall antioxidant defenses. These results support the use of this plant against anti-inflammatory diseases in traditional medicine.&#x0D; Keywords: Centaurea calcitrapa L., in vivo-antioxidant activity, DPPH, hemolysis, flavonoids.

Could Sumac Be Effective on COVID-19 Treatment?

Sumac is an herbal product, commonly consumed as a spice and was used for medical treatment for centuries. The phytochemical structure of Sumac was studied extensively, and it was established that the herb contained tannins, polyphenols, flavonoids, organic acids, and essential oils. Various scientific studies demonstrated that Sumac had a free oxygen radical-scavenging effect, a protective effect against liver damage, antihemolytic, leukopenia, and antifibrogenic effects, along with its antiviral, antimicrobial, anti-inflammatory, and antioxidant properties. Recently, several scientific studies described the pathophysiology, clinical course, and the treatment of COVID-19 infection. The examination of the characteristics of COVID-19 infection revealed via the clinical studies suggests that Sumac extract could be useful in the treatment of COVID-19. Given the scientific studies focusing on the beneficial effects of Sumac, the present review aims to provide an encouraging viewpoint to investigate whether Sumac is effective in treating COVID-19 infection.

Polyphenols of jabuticaba [Myrciaria jaboticaba (Vell.) O.Berg] seeds incorporated in a yogurt model exert antioxidant activity and modulate gut microbiota of 1,2-dimethylhydrazine-induced colon cancer in rats

The chemical composition, antioxidant activity (AA), cytotoxic activity, antihemolytic effects, and enzyme inhibition (EI) of lyophilized jabuticaba (Myrciaria jaboticaba) seed extract (LJE) was studied. The main compounds found were castalagin, vescalagin, procyanidin A2, and ellagic acid. LJE was more toxic to cancer cells than to normal cells, meaning relative toxicological safety. This cytotoxic effect can be attributed to the pro-oxidant effect observed in the reactive oxygen species (ROS) generation assay. LJE inhibited α-amylase, α-glucosidase, and ACE-I activities and protected human erythrocytes from hemolysis. LJE was incorporated into yogurts at different concentrations and the total phenolic content, AA, and EI increased in a dose-dependent manner. LJE-containing yogurt presented 86% sensory acceptance. The yogurt was administered to Wistar rats bearing cancer and it modulated the gut bacterial microbiota, having a prebiotic effect. LJE is a potential functional ingredient for food companies looking for TPC, AA, and prebiotic effect in vivo.