Trained Animals Research Articles

FMD virus spillover from domestic livestock caused outbreak in captive wild ungulates: First report from Pakistan

The potential spillover of foot and mouth disease (FMD) virus at the wildlife-livestock interface is mainly responsible for the outbreaks in captive wild ungulates. The current study was planned to investigate an FMD outbreak in the wild ungulate species in the Jallo Wildlife Park and breeding facility, Lahore, Pakistan from Mar 2021– Jun 2021. The disease was confirmed based on ELISA through the detection of antibodies against the non-structural protein of FMD virus and typical clinical signs of oral and feet lesions, and even partial or complete hoof shedding in severe cases. To investigate the possible cause of FMD spillover and its pattern, a series of interviews were conducted with wildlife practitioners, animal handlers, and the local veterinarians in villages adjacent to the park. The data revealed neither vaccination nor any FMD outbreak in the last 10 years. The epidemic curve and time-series analysis showed a mixed outbreak characterized by the introduction of disease and then propagative spread from infected to susceptible animals. After the first reports of clinical signs in Blackbuck, Urial and Mouflon sheep were infected while in the end, the cases were reported from the enclosures of Sambar deer and Spotted deer. The morbidity rate among all the wild ungulate species was 92% and the mean mortality rate was 27%. The study concluded that possible sources for primary disease incidence might be animal handlers having FMD infected animals in their houses and transport vehicles coming from adjacent disease-affected villages. For the disease spread between enclosures, another possibility of wind-borne FMD virus spread could also be considered. This is the first report regarding the FMD outbreak and its spillover pattern in captive wild ungulates in Pakistan. These findings will be helpful in understanding the importance of wildlife livestock interface in FMD transmission and to design effective strategies to control the disease.

Eliciting and Characterizing Porcine Vocalizations: When Pigs Fly

While voice-related therapeutic interventions are often researched preclinically in the porcine model, there are no well-established methods to induce porcine glottic phonation. Described approaches, such as training animals to phonate for positive reinforcement are time-consuming and plagued by inherent variability in the type of phonation produced and contamination of background noise. Thus, a reliable method of assessing glottic phonation in the porcine model is needed. In this study, we have created a novel pulley-based apparatus with harness for "pig-lifting" with surrounding acoustic insulation and high-directional microphone with digital recorder for recording phonation. Praat and Matlab were used to analyze all porcine vocalizations for fundamental frequency (F0), intensity, duration of phonation and cepstral peak prominence (CPP). Glottic phonation was detected using F0 (≥2000 hz), duration (≥3 seconds) and researcher perceptual judgment. Partial-glottic phonations were also analyzed. Reliability between researcher judgment and acoustic measures for glottic phonation detection was high. Acoustic analysis demonstrated that glottic and partial-glottic phonation was consistently elicited, with no formal training of the minipigs required. Glottic vocalizations increased with multiple lifts. Glottic phonation continued to be elicited after multiple days but became less frequent. Glottic and partial-glottic phonations had similar CPP values over the 6 experimental days. Our cost-effective, reliable method of inducing and recording glottic phonation in the porcine model may provide a cost effective, preclinical tool in voice research.

Effect of cooperative care training on physiological parameters and compliance in dogs undergoing a veterinary examination – A pilot study

One way to reduce fear and lack of compliance during veterinary procedures is ‘cooperative care training’, training animals to voluntarily participate in husbandry and medical care. Here we assessed the effects of cooperative care training on heart rate (HR), heart rate variability (HRV), tympanic membrane temperature (TMT) and compliance (i.e., all steps of the examination completed) of dogs during a veterinary examination. A blinded controlled trial with 47 dogs (training group (TG): 26; control group (CG): 21) was carried out. Forty dogs completed the study (TG: 22, CG:18). Dogs and their owners took part in a standardized veterinary visit twice (visit interval: 140 ± 23 days). In between, the TG took part in cooperative care training (10 ± 2 group training sessions, additional training at home). Linear mixed models were calculated (fixed effects HR/HRV: group* visit* period, TMT: group* visit; random effect: dogID). Compliance (completed steps of exam) was analyzed using McNemar tests. HR/HRV was higher/lower during the veterinary examination compared to the waiting room (period: p < 0.001), indicating that the examination was more stressful. No significant differences in HR/HRV regarding group*visit were found. TMT, taken at the end of each examination, resulted in a significant group*visit interaction (p = 0.008); stress appeared increased during visit 2 in the CG whereas in the TG a heterogeneous pattern, with 42% of dogs with reduced rightward bias, was observed. Compliance was lower in the TG during the second visit (p = 0.04). Trainer and owners claimed observable improvement of behavior during training and a decrease in HR between visits correlated significantly with trainer perception of high training success (rs = −0.52, p = 0.049). Overall, transfer of trained skills to the veterinary examination performed by a team blinded to the group allocation was poor. TMT analyses and HR/HRV data within the training group, suggest that the training had differing effects on the dogs based on their previous tolerance of handling and their progress made during training. Further research how to optimize training programs, including desensitization and counterconditioning, and their outcomes is needed.

First report of Rickettsia asembonensis in small ruminants.

Rickettsioses is a group of emerging infectious diseases in Southeast Asia caused by Gram-negative obligate intracellular bacteria in the Rickettsiae tribe. However, there is limited information regarding the vertebrate hosts of Rickettsia spp. in this region. This study aims to detect and identify Rickettsia agents present in wildlife and domesticated animals in Malaysia using Polymerase Chain Reaction (PCR) and sequencing of citrate synthase gene (gltA), followed by genotyping and phylogenetic analysis. Rickettsia sp. was detected in 2 (0.67%) of 300 wildlife and domesticated animal blood samples. The positive samples were derived from a goat (5.56% of 18) and a sheep (2.22% of 45). Both sequences demonstrated 99.64% sequence similarity to Rickettsia asembonensis, a species that is known to infect humans and macaques. This study reported for the first time the detection of R. asembonensis in sheep and goats in Malaysian farms, suggesting this species may be adapting to a wider range of animals, specifically farm animals. Therefore, this bacterium may pose a zoonotic threat to the local community particularly to the farmworkers or animal handlers. The low infection rate of this pathogen across different animals highlighted the need of continuous surveillance of emerging and reemerging pathogens among animal populations.

Development of Desirable Behaviors in Dog-Assisted Interventions.

Simple SummaryIn a recent paper, Hall et al. encouraged professionals of canine training to share their observations and procedures with researchers in the field of dog learning and cognition, with the goal of coordinating knowledge and make better use of time and resources. In response to this invitation, here, we present an integrative method for the training of dogs that take part in animal-assisted interventions (AAI). This method has been developed taking into account the needs observed during nearly 30 years of interventions for the cognitive, relational, functional, and emotional improvement of the users. This method focuses on the dog, developing in the animals the necessary skills for their inclusion in dog-assisted interventions from a constructivist perspective, while guaranteeing their well-being during the training and the execution of their tasks.Dog-assisted interventions (DAI) are those that include specially trained dogs in human health services. Often, the training methods employed to train animals for DAI are transmitted between trainers, so the latest scientific research on dog learning and cognition is not always taken into account. The present work aims to evaluate the impact that the main theories on the evolution of the dog have had both in promoting different training methods and in the relevance of behavior in the evolution of the skills of actual dogs. Then, an integrative method for the training of dogs is presented. This method takes into account the research on dog learning mechanisms and cognition processes, and effectively promotes the development of desirable behaviors for DAI during the dog’s ontogeny.

Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization.

Staphylococcus aureus (S. aureus) infections, particularly methicillin-resistant Staphylococcus aureus (MRSA) in humans and animals, have become a significant concern globally. The present study aimed to determine the prevalence and antibiogram of S. aureus isolated from animal handlers in Peninsular Malaysia. Furthermore, the genotypic characteristics of S. aureus isolates were also investigated. Nasal and oral swab samples were collected from 423 animal handlers in Peninsular Malaysia. The antibiogram profiles of S. aureus against 18 antibiotics were established using a Kirby–Bauer test. The genotypic profile of S. aureus, including the presence of antimicrobial resistance (AMR), virulence genes and spa genotypes, was investigated using molecular techniques. The overall carriage rate of S. aureus, MRSA and MDRSA was 30.5%, 1.2% and 19.4%, respectively. S. aureus was highly resistant against penicillin (72.3%) and amoxicillin (52.3%). Meanwhile, gentamicin and linezolid were fully effective against all the isolated S. aureus from animal handlers. It was observed that animal handlers with close exposure to poultry were more likely to carry S. aureus that is resistant to tetracycline and erythromycin. S. aureus isolates harboured tetracycline resistance (tetK, tetL and tetM), erythromycin resistance (ermA, ermB, ermC and msrA) and immune evasion cluster (IEC) genes (scn, chp, sak, sea and sep). Seventeen different spa types were detected among the 30 isolates of MDRSA, with t189 (16.7%) and t4171 (16.7%) being the predominant spa type, suggesting wide genetic diversity of the MDRSA isolates. The present study demonstrated the prevalence of S. aureus strains, including MRSA and MDRSA with various antimicrobial resistance and genetic profiles from animal handlers in Peninsular Malaysia.

Occurrence, molecular characterization and antimicrobial-resistance pattern of Staphylococcus species isolates from buck semen

Staphylococcus aureus is one of the most prevalent pathogens, and a causative agent of a variety of infections in humans and animals. Most studies concentrated on characterization of staphylococcus isolates and its antimicrobial resistance from various illness of veterinary importance, but there is no specific study that is available on isolates from reproductive tract of small ruminants and especially its semen. Hence, in the current study, a total of 48 semen samples were collected from healthy bucks of different breeds to investigate the occurrence of S. aureus. Antimicrobial resistance and virulence of the Staphylococcus isolates were determined to assess the adverse effects of them on buck fertility. The bacterial isolates were tentatively confirmed as Staphylococcus spp. based on the Gram's staining, growth on Mannitol salt agar and catalase test. Overall, 75% (n = 36) of the samples were positive for Staphylococcus spp. from the total 48 buck semen ejaculates from different breeds and among them 23 (63.89%) were coagulase-negative (CoNS) and 13 (36.11%) were coagulase-positive Staphylococcus (CoPS) strains. The species identified by molecular characterization are S. aureus, S. chromogenes, S. haemolyticus, S. sciuri, S. simulans, and S. epidermidis from buck semen. Further, these isolates exhibited varying degrees of multidrug resistance genotypically as well as phenotypically. The presence of antibiotic resistance and virulence genes may pose a potential threat to reproductive health of animals, the animal handlers and livestock keepers, while simultaneously highlighting the need for vigilant monitoring of these isolates at the time of semen cryopreservation.

ADVANCES IN VETERINARY EDUCATION THROUGH NON-ANIMAL MODELS

PURPOSE: With the recognition of animals as sentient beings and the growing research in the field of animal welfare the use of live animals for scientific purposes and education has been under question. The present paper focused on the legal framework for the protection of animals used for research and education while giving examples on their replacement with alternative methods in undergraduate veterinary courses. METHODS: Analysis is made on the implemented legal framework which lays down the requirement for animal protection and specifically the 3Rs principles for animal experimentation. Additionally, a short literature review is made to identify the most common educational approaches used to replace live animals in veterinary training. RESULTS: Based on the review we identified common ethical dilemmas for animal use in education like ethically sourced cadavers, continuous tests and manipulations during the process of training of veterinary students and presented as well some examples on alternatives like simulators, artificial models and digital resources. CONCLUSIONS: Through the development and implementation of modern educational resources aimed at the replacement of live animals with non-animal alternatives veterinary students could benefit in their studies both in terms of solving ethical issues regarding animal use and in gaining self-confidence, improvement of skills, and performance.

Ultrasound evaluation of digital flexor tendons of jumping horses undergoing treadmill training.

This study aimed to perform ultrasonographic evaluation of the digital flexor tendons of jumping horses undergoing complementary training on a treadmill. Fifteen Brazilian Sport horses were divided into two groups: Group I with 7 animals in physical training complementary to the standard training at the Brazilian Army’s Riding School; and Group II with 8 animals in standard training used in the Brazilian Army’s Riding School. Complementary training was performed on a treadmill on an inclined plane. Ultrasonographic images were taken at the beginning and at the end of training. The left thoracic and pelvic limbs of horses were evaluated for echogenicity values and cross-sectional areas of the superficial digital flexor tendons (SDFTs) and deep digital flexor tendons (DDFTs) in zones IA, IIB, and IIIB. The mean areas of the SDFT were 93.7 mm2 and 95.8 mm2 at the beginning and the end of training, respectively. The cross-sectional area of DDFT increased from 124.6 mm2 at the beginning of training to 136.8 mm2 at the end of training. The echogenicity values of both tendons were between 0 and 1 as expected, concluding that clinically, the animals showed adaptation to the exercise protocol with no signs of tendon injury being detected, implying that traditional training and complementary training are safe.

Ethical Conduct with Invertebrate Animals: Routes for Inclusive, Humanitarian, and Sustainable Education

Perceptions of nature depend on experiences relating to biodiversity, thus this study investigated the representation of invertebrates in different sections of Brazilian society by means of a questionnaire available from March to December 2015 as a resource in terms of the conservation of nature and human health in order to design an ethical values guide for this relationship. This study tested the hypotheses that there are differences in the conception and representation: a) associated with gender, educational level, use of animals in vocational training, tutoring, and housing area; b) according to the role assigned to invertebrates as a resource, including in conservation and health; and c) that the different representations result in different value assignments used to intermediate decisions on how, when, and why to use invertebrates. Thus, through quantitative and cross-sectional research, our objective was to identify the popular representation of invertebrates. The analysis of the results of an online questionnaire with 281 respondents showed definitions and concepts that were predominantly similar to the tested variables, which suggests that the present study provides evidence for the understanding of the ecological importance of the invertebrates, the belief in their sentience, and disagreement with the substitutive use of vertebrates in experimentation. However, the naturalistic and ecological positions regarding nature prevailed, indicating the persistence of utilitarian values. The results cast interpretive clues that the only by cooperation between education and environmental bioethics will possible the confluence of values and interests of animals and human in favor of a critical, ethical, and sustainable society.

Prevalence of coagulase‐positive methicillin‐resistant Staphylococcus aureus and Staphylococcus pseudintermedius in dogs in Bangladesh

BackgroundThe emergence of methicillin‐resistant Staphylococcus aureus (MRSA) and methicillin‐resistant Staphylococcus pseudintermedius (MRSP) have a significant health impact on people with direct or supportive occupations in veterinary medicine including veterinarians, animal handlers, laboratory personnel and pet owners.ObjectivesThis cross‐sectional survey was conducted to determine the prevalence of and risk factors for S. aureus, S. pseudintermedius, MRSA and MRSP in dogs in Bangladesh.MethodsA total of 358 swab samples were collected from different body sites of 150 dogs attending a university teaching veterinary hospital between January and June 2018. Standard bacteriological procedures were followed to isolate Staphylococcus, and identification was confirmed to the species level by PCR to detect the nuc gene. MRSA and MRSP were confirmed by the presence of the mecA gene.ResultsThe prevalence of coagulase‐positive S. aureus and S. pseudintermedius in dogs were 16% and 45.3%, respectively. S. aureus and S. pseudintermedius isolates displayed the highest resistance against nalidixic acid (95.2% and 91%, respectively) and erythromycin (89.3% and 84.7%, respectively). Notably, all the staphylococcal isolates showed resistance to ≥3 antimicrobial classes. The prevalence of MRSA and MRSP in dogs was 8.7% and 6%, respectively. Multivariable logistic regression analysis identified the following variables as risk factors for MRSA colonisation in dogs: dogs with dermatitis (odds ratio [OR], 12.24, 95% CI: 3.12–57.33; p < 0.001) and history of antibiotic use (OR 8.73, 95% CI: 2.23–43.10; p < 0.001). Presence of otitis (OR 14.22; 95% CI: 1.64–103.58; p = 0.008) and oral lesions (OR 9.48, 95% CI: 1.14–64.82; p = 0.002) were identified as the significant risk factors for the carriage of MRSP.ConclusionsThe circulation of multidrug‐resistant S. aureus and S. pseudintermedius is a serious concern to dogs and humans. To our knowledge, this is the first report of S. pseudintermedius and MRSP affecting dogs in Bangladesh.

Brucellosis in India: Comparing exposure amongst veterinarians, para-veterinarians and animal handlers

The exposure to Brucella species is an occupational hazard for veterinary personnel in India. In our previous study, paraveterinarians and animal handlers were found to be at higher risk of being seropositive. In the present study, we further investigate comparative exposure risk to brucellosis amongst the veterinary professionals, identify risk factors, and evaluate the perceptions and practices towards using adequate personal protective equipment (PPE) while attending high risk veterinary interventions. A cross-sectional study was conducted amongst veterinary personnel in the Punjab state of India. Logistic and negative binomial regression models were constructed to interpret the influence of categorical and numerical variables on prevalence of brucellosis and the adequacy of PPE use, respectively. Compared to veterinarians, animal handlers were less likely to consider veterinary practice in sheep and goats (odds ratio [OR] 0.3; 95% confidence interval [CI]: 0.2–0.7) or assisting in obstetric interventions (OR 0.3, 95% CI 0.1–0.8) as potential exposures. They were less aware of the signs and symptoms of the disease in humans (OR 0.05, 95% CI 0.02–0.14) and of the availability of vaccines for cattle (OR 0.07, 95% CI 0.01–0.3) than the veterinarians, and were least likely of the three groups to recommend vaccination of cattle against brucellosis to animal owners (OR 0.1, 95% CI 0.02–0.3). Compared to veterinarians, they were also less likely to consider zoonotic risk (OR 0.4, 95% CI 0.2–0.8) or better efficiency (OR 0.1, 95% CI 0.05–0.4) as enablers of PPE use during veterinary procedures. Of the three occupational groups, animal handlers were also less likely to use adequate PPE during high-risk veterinary exposures, although they were found to have handled the highest number of cases of abortion. The average use of adequate PPE during high-risk interventions in one month preceding this study was lowest for animal handlers. Veterinary personnel with a higher rate of PPE use during high-risk veterinary interventions were likely to obtain information on brucellosis from government sources (p = 0.06) and felt that PPE use enhanced their professional efficiency (Incidence rate ratio [IRR] 3.3, 95% CI 1.5–8.1) compared to those who were less likely to use adequate PPE. We recommend concerted efforts to increase awareness amongst veterinary personnel, particularly amongst animal handlers, regarding brucellosis and the importance of biosecurity measures in veterinary practice. Designing training courses on the importance of PPE use along with formulation of biosecurity guidelines at local levels could help reduce the prevalence of the disease in veterinary personnel.

Development of an Animal-Assisted Activity/Therapy Dog Checklist for Long-Term Care Facilities

Animal-assisted activities (AAA) and therapy standards of practice have been published to protect the well-being of animals, animal handlers, and the special populations of patients that benefit from this mode of treatment. Inconsistencies among practice standards with concerns surrounding the topics of dog welfare, human well-being, and zoonotic transmission have been reported. The purpose of this qualitative research study was to review published AAA and therapy standards with older adult populations for best practices, conduct focus group sessions with caregivers from long-term care facilities that allow therapy dog visitation, and synthesize findings into an AAA checklist to be used by long-term care facility decision-makers when interviewing or bringing in therapy dog teams. Comparative analyses utilizing a systematic and sequential approach was used to analyze the data from the focus group sessions. Due to the COVID-19 pandemic, only two focus group sessions at one long-term care facility were conducted resulting in a total of 15 caregivers. Four themes emerged from the data: promotes positive mood, essential resident screenings, caregiver roles, and memory aides. Relevant themes and AAA and therapy standards and guidelines were then combined in the development of the AAA/Therapy Dog Checklist. Administrators may find having a user-friendly AAA/therapy dog checklist a useful tool that can be used when interviewing therapy dog teams to ensure future dog therapy experiences will be positive and safe. The safety and well-being of residents in long-term care facilities and animals are essential to promote positive health outcomes for both populations.

Alternatives in Education-Evaluation of Rat Simulators in Laboratory Animal Training Courses from Participants' Perspective.

Simple SummaryTraining on live animals in laboratory animal science (LAS) courses is legally defined as an animal experiment. For stringent implementation of the 3R (replace, reduce, refine) principle, five rat simulators are currently available which provide training of handling and routine procedures. As these simulators seem to have great benefit for all users, the aim of this study is to investigate the simulators’ impact on the 3R principle from the course participants’ perspective, who can best evaluate their learning efficacy which, in turn, defines their 3R potential. Thus, the simulators were evaluated by 332 course participants of 27 specialized LAS courses by completing a practical training workshop and a paper-based two-part questionnaire, integrated in the official course schedule. The results revealed strong support for simulator-based training and it was considered a useful supplement in LAS training. However, the simulators currently available may not completely replace training on a live animal and improvements are necessary. As these results are also reflected in literature data on simulator training in other fields of education and training, more research regarding novel simulators and their development is needed, in order to ensure an even more comprehensive protection of laboratory animals in education and training in future.In laboratory animal science (LAS) education and training, five simulators are available for exercises on handling and routine procedures on the rat, which is—beside mice—the most commonly used species in LAS. Since these simulators may have high potential in protecting laboratory rats, the aim of this study is to investigate the simulators’ impact on the 3R (replace, reduce, refine) principle in LAS education and training. Therefore, the simulators were evaluated by 332 course participants in 27 different LAS courses via a practical simulator training workshop and a paper-based two-part questionnaire—both integrated in the official LAS course schedule. The results showed a high positive resonance for simulator training and it was considered especially useful for the inexperienced. However, the current simulators may not completely replace exercises on live animals and improvements regarding more realistic simulators are demanded. In accordance with literature data on simulator-use also in other fields of education, more research on simulators and new developments are needed, particularly with the aim for a broad implementation in LAS education and training benefiting all 3Rs.

Automated lever task with minimum antigravity movement for rats with cervical spinal cord injury

BackgroundAlthough there is currently no cure for paralysis due to spinal cord injury (SCI), the highest treatment priority is restoring arm and hand function for people with cervical SCI. Preclinical animal models provide an opportunity to test innovative treatments, but severe cervical injury models require significant time and effort to assess responses to novel interventions. Moreover, there is no behavioral task that can assess forelimb movement in rats with severe cervical SCI unable to perform antigravity movements. New methodWe developed a novel lever pressing task for rats with severe cervical SCI. We employed an automated adaptive algorithm to train animals using open-source software and commercially available hardware. We found that using the adaptive training required only 13.3 ± 2.5 training days to achieve behavioral proficiency. The lever press task could quantify immediate and long-term improvements in severely impaired forelimb function effectively. This behavior platform has potential to facilitate rehabilitative training and assess effects of therapeutic modalities following SCI. Comparison with existing methodsThere is no existing assessment aiming to quantify forelimb extension movement in rodents without function against gravity. We found that the new lever press task in the antigravity position could assess the severity of cervical SCI as well as the compensatory movement in the proximal forelimb less affected by the injury. ConclusionsThis study demonstrates that the new behavioral task is capable of tracking the functional changes with various therapies in rats with severe forelimb impairments in a cost- and time-efficient manner.

The Journal of the Experimental Analysis of Behavior announces a special issue on the topic of: Strengthening the Research‐Practice Loop in Applied Animal Behavior

Journal of the Experimental Analysis of BehaviorVolume 116, Issue 3 p. 397-397 Call for Papers The Journal of the Experimental Analysis of Behavior announces a special issue on the topic of: Strengthening the Research-Practice Loop in Applied Animal Behavior First published: 12 November 2021 https://doi.org/10.1002/jeab.728Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume116, Issue3November 2021Pages 397-397 RelatedInformation

Endurance training increases ventricular refractoriness and wavelength of the cardiac impulse without participation of parasympathetic postganglionic neurons. A study in isolated rabbit heart

Abstract Background Endurance physical training plays a protective role in against ventricular fibrillation (VF), but the exact underlying mechanisms are not completely understood. It is well-known that modifications in myocardial ventricular properties such as refractoriness, conduction velocity and wavelength are key in the initiation and maintenance of VF; furthermore, vagus nerve stimulation has prophylactic effects on malignant arrhythmias and VF. On the other hand, parasympathetic nervous system activity is increased in trained individuals, which in turn affects different ventricular electrophysiological properties. We hypothesized that physical training increases conduction velocity and wavelength, and that these changes are mediated by myocardial cholinergic neurons. Methods To test this hypothesis, ten rabbits were submitted to a six-week endurance training protocol and twenty controls were not trained (divided in control group, n=10 and sham group n=10). After training, rabbits were euthanized and their hearts excised, isolated and perfused in a Langendorff system. A pacing electrode and a plaque with 240 recoding electrodes acquiring at 1 KHz were positioned on the left ventricle (LV). Extraestimulus test using four different pacing cycle lengths (90% basal cycle length, 250, 200 and 150 ms) was performed before and after atropine (1μM, control and trained groups) or vehicle (tyrode, sham group) infusion. We studied 1) LV effective refractory period (ERP), 2) LV functional refractory period (FRP), 3) LV conduction velocity (CV), and 4) LV wavelength, determined as LV FRP x CV. Factorial ANOVA (mixed model) was used for statistical analysis (p&amp;lt;0.05). Results Before parasympathetic blockade, LV FRP increased in trained animals (Figure, B) whereas no difference was found in LV CV between trained and control animals at any pacing cycle length (Figure, A). In consequence, LV wavelength increased in trained animals (Figure, C). There were no changes in LV ERP, FRP, CV and wavelength when comparisons were made within groups before and after atropine infusion. In sham animals, vehicle infusion or time-course of the experiment did not modify LV FRP, ERP, CV and wavelength. Conclusion Physical training increases LV wavelength, which can be one electrophysiological mechanism by which endurance training could protect against VF. Since modifications of ventricular refractoriness and wavelength do not seem dependent of intrinsic parasympathetic nervous system activity, other intrinsic mechanisms could be implied and warrant further research. Funding Acknowledgement Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Instituto de Salud Carlos III, Generalitat Valenciana

Bulls for sacrifice, cows for work? Morphometric models suggest that female cattle were used for traction in the Chinese Bronze Age Late Shang dynasty (ca. 1300–1046 BCE)

Training animals to pull agricultural equipment and wheeled transport significantly shaped and advanced human economic systems. In this context the use of large domestic animals such as cattle was a milestone event in human history, part of what Sherratt memorably termed the Secondary Products Revolution: the use of the products of live animals such as milk, wool and traction power. It is commonly assumed that male cattle were generally preferred for traction because of their greater strength compared with females, and the importance of the latter for breeding and, in some societies, for milk and for dairy products, but surprisingly little is known of this aspect of the Secondary Products Revolution in prehistoric China. Here we apply established morphometric models to 10 assemblages of cattle bones from Chinese Bronze Age (ca. 2000–221 BCE) contexts. Our results indicate a process of intensified cattle labour exploitation at this time and, intriguingly, we also observe the earliest labour employment of female cattle during the Late Shang dynasty (ca. 1300–1046 BCE). It is proposed that female cattle may have been required for traction because of the large numbers of male cattle, especially bulls, that were sent for ritual sacrifice. Such a strategy reveals a sophisticated social management, upon which the Late Shang civilisation eventually developed.

A Retrospective Study on Wild Orphan Animals in Afyon Kocatepe University Wildlife Rescue Rehabilitation, Training, Practice and Research Center (AKUREM)

In the article published in Kocatepe Veterinary Journal with the "Kocatepe Vet J (2020) 13(3):272-280" and the DOI number "10.30607/kvj.766330", the "Acknowledgements" section was inadvertently forgotten and the following text must be add. ACKNOWLEDGEMENT This Project was supported by the scientific research committe of Afyon Kocatepe University (Project no: 18.KARIYER.286). The authors thank to Afyon Kocatepe University Wildlife Rescue Rehabilitation Training Practice and Research Center, Afyon Kocatepe University Veterinary Health Practice and Research Center and 5th District Directorate of Nature Conservation and National Parks for their cooperations.

Sensitive and robust chemical detection using an olfactory brain-computer interface

When it comes to detecting volatile chemicals, biological olfactory systems far outperform all artificial chemical detection devices in their versatility, speed, and specificity. Consequently, the use of trained animals for chemical detection in security, defense, healthcare, agriculture, and other applications has grown astronomically. However, the use of animals in this capacity requires extensive training and behavior-based communication. Here we propose an alternative strategy, a bio-electronic nose, that capitalizes on the superior capability of the mammalian olfactory system, but bypasses behavioral output by reading olfactory information directly from the brain. We engineered a brain-computer interface that captures neuronal signals from an early stage of olfactory processing in awake mice combined with machine learning techniques to form a sensitive and selective chemical detector. We chronically implanted a grid electrode array on the surface of the mouse olfactory bulb and systematically recorded responses to a large battery of odorants and odorant mixtures across a wide range of concentrations. The bio-electronic nose has a comparable sensitivity to the trained animal and can detect odors on a variable background. We also introduce a novel genetic engineering approach that modifies the relative abundance of particular olfactory receptors in order to improve the sensitivity of our bio-electronic nose for specific chemical targets. Our recordings were stable over months, providing evidence for robust and stable decoding over time. The system also works in freely moving animals, allowing chemical detection to occur in real-world environments. Our bio-electronic nose outperforms current methods in terms of its stability, specificity, and versatility, setting a new standard for chemical detection.