Analytical Performance Research Articles

LC-MS and High-Throughput Data Processing Solutions for Lipid Metabolic Tracing Using Bioorthogonal Click Chemistry.

Tracing lipid metabolism in mammalian cells presents a significant technological challenge due to the vast structural diversity of lipids involved in multiple metabolic routes. Bioorthogonal approaches based on click chemistry have revolutionized analytical performance in lipid tracing. When adapted for mass spectrometry (MS), they enable highly specific and sensitive analyses of lipid transformations at the lipidome scale. Here, we advance this approach by integrating liquid chromatography (LC) prior to MS detection and developing a software-assisted workflow for high-throughput data processing. LC separation resolved labeled and unmodified lipids, enabling qualitative and quantitative analysis of both lipidome fractions, as well as isomeric lipid species. Using synthetic standards and endogenously produced alkyne lipids, we characterized LC-MS behavior, including preferential adduct formation and the extent of in-source fragmentation. Specific fragmentation rules, derived from tandem MS experiments for 23 lipid subclasses, were implemented in Lipostar2 software for high-throughput annotation and quantification of labeled lipids. Applying this platform, we traced metabolic pathways of palmitic and oleic acid alkynes, revealing distinct lipid incorporation patterns and metabolic bottlenecks. Altogether, here we provide an integrated analytical and bioinformatics platform for high-throughput tracing of lipid metabolism using LC-MS workflow.

LC‐MS and High‐Throughput Data Processing Solutions for Lipid Metabolic Tracing Using Bioorthogonal Click Chemistry

Abstract Tracing lipid metabolism in mammalian cells presents a significant technological challenge due to the vast structural diversity of lipids involved in multiple metabolic routes. Bioorthogonal approaches based on click chemistry have revolutionized analytical performance in lipid tracing. When adapted for mass spectrometry (MS), they enable highly specific and sensitive analyses of lipid transformations at the lipidome scale. Here, we advance this approach by integrating liquid chromatography (LC) prior to MS detection and developing a software‐assisted workflow for high‐throughput data processing. LC separation resolved labeled and unmodified lipids, enabling qualitative and quantitative analysis of both lipidome fractions, as well as isomeric lipid species. Using synthetic standards and endogenously produced alkyne lipids, we characterized LC‐MS behavior, including preferential adduct formation and the extent of in‐source fragmentation. Specific fragmentation rules, derived from tandem MS experiments for 23 lipid subclasses, were implemented in Lipostar2 software for high‐throughput annotation and quantification of labeled lipids. Applying this platform, we traced metabolic pathways of palmitic and oleic acid alkynes, revealing distinct lipid incorporation patterns and metabolic bottlenecks. Altogether, here we provide an integrated analytical and bioinformatics platform for high‐throughput tracing of lipid metabolism using LC‐MS workflow.

Novel multiparameter optical sensor head design for marine environments.

Anthropogenic activities have led to increased stress on our marine and other aquatic environments. There is a pressing need to monitor, measure, understand and mitigate causes of these pressures. This paper presents a novel optical head for monitoring and measuring marine based optical phenomena. The development and preliminary testing of the optical head were designed to detect optically active constituents in the marine and coastal environments. Potential applications may include the detection of Harmful Algal Blooms (HAB), which due to their production of toxins have deleterious effects on marine ecosystems, dissolved organic matter (DOM), oil spills, through the measurement of dissolved fluorescent petroleum compounds and turbidity, a key metric in marine and water quality measurements. Preliminary laboratory based results indicate that the optical head is well suited for measuring in-vivo Chlorophyll a (Chl a) fluorescence, turbidity, fluorescent dissolved organic matter (fDOM) and petroleum. For turbidity and in-vivo Chl a, analytical performance was benchmarked against off-the-shelf commercial sensors. The developed optical head demonstrates good analytical performance with certified reference standards and a very good agreement with the reference instrument.

Lateral flow assay with automatic signal amplification based on delayed substrate release.

Lateral flow assay with automatic signal amplification based on delayed substrate release.

Qualitative and quantitative lipidomic profiling of cardiolipin, oxidized cardiolipin, and monolysocardiolipin from human skeletal muscle by UPLC-Orbitrap-MSn.

Qualitative and quantitative lipidomic profiling of cardiolipin, oxidized cardiolipin, and monolysocardiolipin from human skeletal muscle by UPLC-Orbitrap-MSn.

RGO/HfO2 nanocomposite-enabled electrochemical sensor for high-performance pesticide detection.

rGO/HfO2 nanocomposite-enabled electrochemical sensor for high-performance pesticide detection.

3D DNA walkers integrated with self-reporting MOFs: Pioneering ratiometric electrochemical sensing for Staphylococcus aureus.

3D DNA walkers integrated with self-reporting MOFs: Pioneering ratiometric electrochemical sensing for Staphylococcus aureus.

Simultaneous sensitive detection and imaging of dual microRNAs through DNA tetrahedral scaffold-corbelled autonomous-motion molecular machine.

Simultaneous sensitive detection and imaging of dual microRNAs through DNA tetrahedral scaffold-corbelled autonomous-motion molecular machine.

Use of a centrifuge in solid-phase extraction is a valid platform for cleanup in the high-throughput analysis of chemical contaminants in nonfatty and fatty foods.

Use of a centrifuge in solid-phase extraction is a valid platform for cleanup in the high-throughput analysis of chemical contaminants in nonfatty and fatty foods.

Establishment of the isotope dilution-gas chromatography-tandem mass spectrometry (ID-GC/MS) method for accurate quantification of phenylalanine and its application to value assignment of external quality assessment samples.

Establishment of the isotope dilution-gas chromatography-tandem mass spectrometry (ID-GC/MS) method for accurate quantification of phenylalanine and its application to value assignment of external quality assessment samples.

Clinical Implementation of Expanded Solid Tumor Fusion Detection With Whole-Transcriptome Sequencing at an Academic Cancer Center.

Chromosomal rearrangements drive tumorigenesis through fusion transcripts. Clinical whole-transcriptome sequencing (WTS) enables comprehensive fusion detection, overcoming limitations of targeted methods, but its clinical application has been hindered by technical and cost barriers. We validated a clinical bait-capture WTS assay using 78 solid tumor samples across diverse tissue types, including 59 with known fusions or oncogenic splice variants. Sensitivity was assessed against clinically reported fusions from targeted next-generation sequencing or fluorescence in situ hybridization. Analytical performance was evaluated for sensitivity, specificity, limit of detection, and reproducibility across varying RNA inputs and sequencing conditions. Clinical implementation data were also analyzed. WTS demonstrated a sensitivity of 97.1% and a specificity of 100% for detecting clinically relevant fusions. Detection was robust across tumor types, with RNA input as low as 1 ng. Optimized sequencing thresholds of 60 million reads and 130-bp read length improved performance. The assay was highly reproducible and resistant to common contaminants. Over 6 months of clinical use (410 specimens), WTS identified 29 fusions (7% detection rate), including six clinically relevant fusions undetectable by our institution's previous targeted panel, increasing the fusion yield by 26%. WTS provides high sensitivity, specificity, and expanded fusion detection, supporting its routine clinical adoption. Future work will explore its broader role in integrated genomic profiling and therapeutic guidance.

Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables.

Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables.

Advancing atom probe tomography capabilities to understand bone microstructures at near-atomic scale.

Advancing atom probe tomography capabilities to understand bone microstructures at near-atomic scale.

Clinical validation and evaluation of the EasyNAT Malaria assay and the Alethia Malaria assay in a non-endemic setting: rapid and sensitive assays for detecting Plasmodium spp. in returning travellers.

Clinical validation and evaluation of the EasyNAT Malaria assay and the Alethia Malaria assay in a non-endemic setting: rapid and sensitive assays for detecting Plasmodium spp. in returning travellers.

Study on the improved method for natural gas analytical system performance evaluation

Abstract With the advancement of natural gas energy measurement methods by domestic governments, enterprises, and users, GB/T 28766-2018 Performance Evaluation of Natural Gas Analysis Systems has received increasing attention and widespread application since its release and implementation in 2018. However, most of the gas chromatography used domestically employs a single-point calibration function for sample analysis and cannot implant true regression functions, which significantly limits the application of the standard. Based on the standard method and the current state of the domestic natural gas industry, this paper proposes an improvement measure for the standard method and validates the improved method through actual measurements. The performance evaluation data from nine chromatography demonstrate that the proposed method can effectively identify equipment with issues.

E-Bike Motor Design Based on Analytical and Finite Element Performance Mapping

E-Bike Motor Design Based on Analytical and Finite Element Performance Mapping

Direct rapid analysis of hazardous analytes in aerosol products using gas-flow-assisted electrostatic field-induced spray ionization mass spectrometry.

Direct rapid analysis of hazardous analytes in aerosol products using gas-flow-assisted electrostatic field-induced spray ionization mass spectrometry.

Electropolymerized Molecularly Imprinted Poly(L‐Phenylalanine) Modified Lab‐Made Screen‐Printed Carbon Electrode for Voltammetric Analysis of Acetaminophen

A selective molecularly imprinted polymer (MIP) sensor was constructed on a lab‐made screen‐printed carbon electrode (SPCE) for the electrochemical determination of acetaminophen (AP). Different conductive carbon inks have been investigated for SPCE production. The sensor was prepared by direct electropolymerization of the L‐phenylalanine on bare SPCE in the presence of the template molecule (AP). Physicochemical and morphological characterization studies of the sensor preparation steps were performed, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), conductivity, contact angle, electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). Under optimized experimental conditions, the differential pulse voltammetry (DPV) response was linearly proportional to the AP concentration between 0.2 and 100 μmol L−1 with a limit of detection (LOD) of 30 nmol L−1. The MIP sensor showed good analytical performance, selectivity, and stability. Also, it was successfully used to quantify AP in fortified water samples.

Colorimetric plasmonic sensing of formaldehyde by in situ formation of core-shell bimetallic gold-silver nanoparticles in liquid films.

Colorimetric plasmonic sensing of formaldehyde by in situ formation of core-shell bimetallic gold-silver nanoparticles in liquid films.

Rapid and ultrasensitive activity detection of β-amylase based on cross-linked metal-organic framework nanozyme.

Rapid and ultrasensitive activity detection of β-amylase based on cross-linked metal-organic framework nanozyme.