Analysis Of Elements Research Articles

Genome-wide annotation and comparative analysis of miniature inverted-repeat transposable elements (MITEs) in six pear species.

Through multi-faceted comparative analysis of MITEs across six pear genomes, we revealed their distribution patterns, functional impacts and their significant role as genomic origins for miRNAs, with copy number being the most critical factor for MITE-miRNA transformation, providing valuable insights for future research. Miniature inverted-repeat transposable elements (MITEs) are prevalent in plant genomes and play a significant role in genome evolution and diversity. The availability of high-quality genome sequences for six pear species-Pyrus bretschneideri Rehd cv. 'Dangshan Suli', Pyrus communis L. cv. 'Bartlett', Pyrus pyrifolia Nakai cv. 'Nijisseiki' and 'Cuiguan', Pyrus ussuriensis maxim cv. 'Zhongai No.1', Pyrus betulifolia Bunge cv. 'Duli'-has facilitated the annotation and comparative analysis of MITEs in these species. Consequently, we identified 12,759 intact MITEs belonging to 750 families. Sequence diversity analysis revealed that these MITEs underwent one or two rounds of amplification burst events within the pear genomes. Among them, 10,368 intact MITEs demonstrated collinearity across six pear species. Further investigation indicated that MITEs are predominantly located upstream regions of genes. Notably, 1832 genes exhibited potential regulation (either up-regulation or down-regulation) due to MITE insertions. Additionally, 4421 previously missing genes, disrupted by MITE insertions, were restored and re-annotated. We identified 8855 MITE-miRNAs belonging to 370 families across all six species, with approximately 75% of miRNAs originating from MITEs. Machine learning analysis revealed copy number as the most influential feature for MITE-miRNA transformation, followed by MITE length and structure stability. These findings provide valuable insights into transposable elements' role in shaping genome diversity and their impact on functional genes and miRNA genesis within pear genomes.

Convergence analysis of weak Galerkin finite element variable-time-step BDF2 implicit scheme for parabolic equations

Convergence analysis of weak Galerkin finite element variable-time-step BDF2 implicit scheme for parabolic equations

Integrating analysis of nutrient elements, endogenous phytohormones, and transcriptomics reveals factors influencing variation of growth in height in Pinus yunnanensis Franch.

Integrating analysis of nutrient elements, endogenous phytohormones, and transcriptomics reveals factors influencing variation of growth in height in Pinus yunnanensis Franch.

Evaluation and health risk analysis of potential toxic elements in select foods vended within a university campus in southeastern Nigeria

Evaluation and health risk analysis of potential toxic elements in select foods vended within a university campus in southeastern Nigeria

A Study on the “Truth-Seeking and Utility-Attaining” in Julia Lovell’s Translation of The True Story of Ah Q from the Perspective of Translator Behavior Criticism

This study employs the framework of translator behavior criticism to conduct a systematic examination of Julia Lovell’s English translation of The True Story of Ah Q, utilizing the “intra-translation and extra-translation dichotomy.” The research investigates how extra-translation factors—such as the translator’s identity, translation motivations, and target readership positioning—shape the formation of the translated text. Through an analysis of intra-translation elements, including linguistic stylistic features, strategies for handling culture-loaded terms, and the use of paratexts, the study reveals the dynamic balance of Lovell’s translation within the “truth-seeking—utility-attaining” continuum. The findings demonstrate that as a Western sinologist-translator, Julia Lovell’s cultural identity, combined with the market-oriented approach of Penguin Books, has collectively shaped a distinctly “reader-oriented” characteristic in her translation. In rendering culture-specific items, she predominantly adopts domesticating strategies, enhancing readability through simplified sentence structures and other accessibility measures to achieve utilitarian goals. Simultaneously, the translator retains an appropriate degree of foreignness in the treatment of key cultural concepts, reflecting a dialectical balance between “authorial fidelity” and “reader adaptation.”

Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Begonia semperflorens Reveals Promising Candidates for Heat Tolerance

Begonia semperflorens (B. semperflorens) is a popular ornamental plant widely used in landscapes such as plazas and flower beds, and it is also commonly grown as a potted plant indoors. It is known for its adaptability to high temperatures, drought, and shade. Under heat-tolerant conditions, heat shock transcription factors (HSFs) are key transcriptional regulatory proteins that play crucial roles in cellular processes. Despite extensive studies on the HSF family in various species, there has been no specific analysis targeting B. semperflorens. In this study, we identified 37 members of the BsHSF gene family in B. semperflorens based on its genome scaffold, which are unevenly distributed across the genome. Phylogenetic analysis reveals that these 37 members can be divided into three subfamilies. Analysis of their physicochemical properties shows significant diversity among these proteins. Except for the BsHSFB7 protein located in the cytoplasm, all other BsHSF proteins were found to be nuclear-localized. A comparison of the amino acid sequences indicates that all BsHSF proteins contain a conserved DNA-binding domain structure. Analysis of the promoter cis-acting elements also suggests that BsHSFs may be associated with heat stress and plant secondary metabolism. We further investigated the duplication events of BsHSF genes and their collinearity with genes from other Begonia species. Finally, through real-time quantitative PCR, we examined the expression patterns of the 37 BsHSFs in different plant tissues (roots, stems, leaves, and flowers) and their expression levels under heat stress treatment. The results show that, except for BsHSF29, all BsHSFs were expressed in various tissues, with varying expression levels across tissues. Except for BsHSF33 and BsHSF34, the expression levels of almost all BsHSF genes increased in response to heat treatment. In summary, these findings provide a better understanding of the role and regulatory mechanisms of HSFs in the heat stress response of B. semperflorens and lay the foundation for further exploration of the biological functions of BsHSFs in the stress responses of B. semperflorens.

Paleoenvironment of the Fifth Member of the Ordovician Majiagou Formation, Central Ordos Basin: Evidences From Trace Elements and C–O Isotopes

ABSTRACTCarbonate rocks hold significant importance in investigating depositional environments. This paper reconstructs the paleoenvironment of carbonate rocks of the Majiagou Formation in the Ordos Basin through the analysis of trace elements and carbon–oxygen isotopes. The results indicate that high Sr/Cu ratios, low Rb/Sr ratios and the high paleotemperature (28.3°C) all reveal that carbonate rocks of the Fifth member of Majiagou Formation (the Ma5 member) were formed under arid and hot climatic conditions. V/(V + Ni), V/Cr, Ni/Co and U/Th values indicate that the sedimentary environment of the Ma5 member was oxic–suboxic, likely due to the water‐retreat period during which the sedimentary water was relatively shallow and less reducing. High Sr/Ba ratios, high paleosalinity Z values and salinity S values all suggest that the carbonate rocks formed in a high‐salinity marine environment. Comprehensive studies show that during the deposition of the Ma5 stratum, the basement experienced uplift coupled with regressive sea‐level conditions. Meanwhile, the arid and hot climate, coupled with an oxic water environment, led to increased evaporation, elevated salinity and reduced sedimentation rates, making it unfavourable for the enrichment and preservation of organic matter. However, this evaporative environment promoted early penecontemporaneous dolomitization, enhancing intercrystalline porosity and permeability to develop high‐quality reservoirs in the Ma5 member. These findings provide a geochemical framework for identifying high‐quality carbonate reservoirs. These reservoirs offer storage space for natural gas, contributing to the formation of large‐scale gas deposits in the region.

The effect of adding Sn and Nb on the microstructure and mechanical properties of Ti80Fe20 alloy

Abstract To improve the toughness of Ti–Fe-based alloys, a small amount of Sn and Nb elements was added into the Ti80Fe20 alloy. The microstructure and mechanical properties of Ti–Fe–Sn–Nb samples were investigated. The results indicate that the microstructure of Ti76.8Fe19.2Sn2Nb2 and Ti75.2Fe18.8Sn3Nb3 alloys with excellent mechanical properties is composed of β-Ti matrix, less α-Ti, and more TiFe phases. Based on analysis of energy dispersive spectrometer (EDS), the Sn and Nb elements tend to be distributed in the Ti-rich phase particles, while the Sn and Nb contents are lower in the TiFe phase particles. The true strain of Ti76.8Fe19.2Sn2Nb2 alloy with work hardening is greater than 33%. The elastic modulus of Ti76.8Fe19.2Sn2Nb2 and Ti75.2Fe18.8Sn3Nb3 alloys are 92.9 GPa and 103 GPa, respectively; they are higher than that of human bones (10–30 GPa), but lower than that of commercial Ti-6Al-4V bio alloy (120 GPa). These two alloys are bio alloys with high practical value for hard tissue implants. The fracture mechanism of Ti78.4Fe19.6Sn1Nb1 alloy is cleavage fracture caused by a large amount of hexagonal close-packed α-Ti phases. However, a large amount of plastic β-Ti phases cause the fracture mechanisms of Ti76.8Fe19.2Sn2Nb2 and Ti75.2Fe18.8Sn3Nb3 alloys to be ductile fracture, while a large amount of brittle TiFe and plastic β-Ti phases are the reason for intergranular ductile fracture of Ti73.6Fe18.4Sn4Nb4 alloy.

Characterization and expression of the wall-associated kinase/wall-associated kinase-like (WAK/WAKL) family in response to Botrytis cinerea infection in strawberry (Fragaria×ananassa)

BackgroundGray mold caused by Botrytis cinerea is a major threat to the production of strawberry. An increasing number of studies have reported that wall-associated kinase/wall-associated kinase-like (WAK/WAKL) played an important role in the recognition of oligogalacturonic acids (OGs) and the induction of plant defense, but there have been no systematic studies of FaWAK/FaWAKL in strawberry.ResultsIn this study, we identified 167 FaWAK/FaWAKL gene family members within the strawberry (Fragaria×ananassa) genome. The phylogenetic analysis showed the FaWAK/FaWAKL gene family has been divided into five groups, and they were unevenly distributed on 46 chromosomes. An analysis of the cis-regulatory elements suggested the FaWAK/FaWAKL gene family was more sensitive to abscisic acid and methyl jasmonate. A total of 36 FaWAK/FaWAKL genes were activated by B. cinerea according to an RNA-seq analysis, and 8 of them strongly responded to B. cinerea and exogenous treatment with OGs, particularly FaWAK35. Transient overexpression of FaWAK35 increased the strawberry resistance to B. cinerea.ConclusionThis study conducted a comprehensive analysis of FaWAK/FaWAKL and provides foundational insights for further exploration of FaWAK/FaWAKL genes in strawberry resistance to B. cinerea.

Transcriptional Regulatory Systems in Pseudomonas: A Comparative Analysis of Helix-Turn-Helix Domains and Two-Component Signal Transduction Networks.

Bacterial communities in diverse environmental niches respond to various external stimuli for survival. A primary means of communication between bacterial cells involves one-component (OC) and two-component signal transduction systems (TCSs). These systems are key for sensing environmental changes and regulating bacterial physiology. TCSs, which are the more complex of the two, consist of a sensor histidine kinase for receiving an external input and a response regulator to convey changes in bacterial cell physiology. For numerous reasons, TCSs have emerged as significant targets for antibacterial drug design due to their role in regulating expression level, bacterial viability, growth, and virulence. Diverse studies have shown the molecular mechanisms by which TCSs regulate virulence and antibiotic resistance in pathogenic bacteria. In this study, we performed a thorough analysis of the data from multiple public databases to assemble a comprehensive catalog of the principal detection systems present in both the non-pathogenic Pseudomonas putida KT2440 and the pathogenic Pseudomonas aeruginosa PAO1 strains. Additionally, we conducted a sequence analysis of regulatory elements associated with transcriptional proteins. These were classified into regulatory families based on Helix-turn-Helix (HTH) protein domain information, a common structural motif for DNA-binding proteins. Moreover, we highlight the function of bacterial TCSs and their involvement in functions essential for bacterial survival and virulence. This comparison aims to identify novel targets that can be exploited for the development of advanced biotherapeutic strategies, potentially leading to new treatments for bacterial infections.

Knowledge cooperation networks structure and evolutionary characteristics of international high-speed railway projects

PurposeThis study aims to uncover the structure and evolutionary characteristics of the knowledge cooperation networks within international HSR projects from an industry chain perspective. It specifically examines the cooperative relationships among knowledge subjects, elements, and the interactions between them. The findings are expected to enhance the efficiency of knowledge cooperation in international HSR projects and provide valuable insights for future cooperate efforts.Design/methodology/approachThis study adopts complex network theory, using the cooperative patent application data of international HSR projects from 2003 to 2023. The research period was divided into three stages. Initially, the study constructs two sub-networks: one for knowledge subjects and the other for knowledge elements. The structure and evolutionary characteristics are analyzed from industry chain perspective. Subsequently, establish a dual-layer knowledge cooperation network encompassing both subjects and elements. This paper further examines the discrete characteristics of knowledge subjects.FindingsThe research findings indicate that the knowledge cooperation sub-networks in international HSR projects have exhibited a three-stage expansion trend. The networks demonstrate small-world characteristics, suggesting a “clustering phenomenon” that results in low connection efficiency. Additionally, they exhibit scale-free properties and manifest a “Matthew effect”. Notably, some pivotal nodes serve as bridges that influence the direction and content of knowledge cooperation. Cross-domain cooperation also shows a positive developmental trend. In the dual-layer subject-element coupling network, knowledge subject diversity has increased, while the uniqueness has slightly declined. This indicates that cooperation in international HSR projects fosters the sharing of knowledge elements among different subjects.Practical implicationsThe results contribute to clarifying the connections and evolutionary trend among nodes in the knowledge cooperation network, facilitating more effective improvements in the cooperation quality. They identify core subjects, and support these subjects in selecting partners and developing cooperative strategies. The analysis of key knowledge elements and the exploration of their cooperative directions help knowledge subjects to adjust resource allocation more strategically. These insights offer recommendations for achieving high-level international knowledge cooperation in HSR projects.Originality/valueFirst, this study refines the analysis by adopting an industry chain perspective. This approach offers recommendations strengthening the efficiency of knowledge cooperation within and between the upstream, midstream, and downstream sectors of the international HSR projects. Second, this research explores the dynamic evolution characteristics, providing a deeper understanding of the development trends of knowledge cooperation. It is helpful for knowledge subjects to understand the development direction and key areas of knowledge elements, timely adjust cooperation strategies and seize market opportunities. Third, this study considers the decoupling characteristics of knowledge subjects and elements. Subjects can take different knowledge cooperation measures according to their level of knowledge dispersion characteristics. This provides a new perspective for analyzing cooperation networks in other complex engineering projects.

Comparative Analysis of Engineering Elements in Five Chinese Junior High School Physics Textbooks

Comparative Analysis of Engineering Elements in Five Chinese Junior High School Physics Textbooks

Vestígios de Memória da Representação Descritiva Materializados em Catálogos

Highlight evidence of memory of the practice of descriptive representation materialized in catalogs belonging to informational environments in Salvador / BA. The research is characterized as descriptive, with the adoption of multiple case, documentary and evidentiary methods. Data analysis was based on a qualitative approach. The results showed documents originating from descriptive representation that supported the organization, identification and access to items in the documentary collections, such as bound printed catalogs and separate catalog cards that reveal historical aspects of cataloging, belonging to the Biblioteca Central do Estado da Bahia; Bibliotheca Gonçalo Moniz and Biblioteca do Museu de Arte da Bahia. Given the analysis of the descriptive elements adopted in each device analyzed in this study, we argue that these catalogs and catalog cards materialize traces of memory of the practice of descriptive representation and reveal the social and historical context of their productions and producers.

Micro-mechanical characterisation of 3D-printed composites via nano-indentation and finite-element homogenization techniques: overcoming challenges in orthotropic property measurement

Abstract Mechanical characterisation of 3D-printed composite materials using conventional methods, such as tension and compression tests, faces several challenges, including precise machining of complex geometries, difficulties in testing materials with time-dependent properties, and extensive sample preparation to account for varying build orientations and raster angles. Accurately characterising the mechanical properties of composite constituents is further complicated by their anisotropic nature, visco-plastic behaviour, and phase interactions at the micro-scale. Traditional nano-indentation techniques often suffer from inaccuracies due to pile-up effects and time-dependent deformations in polymer matrices. To overcome these challenges, this study introduces an innovative methodology that integrates nano-indentation and micro-mechanical analysis of a representative volume element (RVE) to determine orthotropic engineering constants. Experimental nano-indentation, coupled with atomic force microscopy, is used to obtain load–displacement curves and residual indentation marks, whilst an inverse finite-element method accounting for neighbouring phase effects and polymer matrix creep properties enhances prediction accuracy. The stiffness properties of composite constituents derived from this method are employed in an RVE-based micro-mechanical model, with effective orthotropic engineering constants validated through experimental tensile and shear tests using the Digital Image Correlation technique. This approach not only enhances micro-mechanical characterisation accuracy but also reduces the need for extensive experimental testing, offering a cost-effective and scalable solution for evaluating 3D-printed composite materials. Additionally, it bridges the gap between microstructural and bulk property measurements, reducing test sample manufacturing costs and minimising the need for repetitive experimental trials.

Resenha da obra teoria da norma jurídica, de Norberto Bobbio

The book Theory of Legal Norms, by Norberto Bobbio, a prominent 20th century philosopher of law, is essential for the theoretical understanding of legal phenomena. Originally published in Italian and later translated into Portuguese, it presents an in-depth and systematic analysis of the main elements that make up legal norms.

Genome-Wide Analysis of the 12-Oxo-Phytodienoic Acid Reductase Gene Family in Peanut and Functional Characterization of AhOPR6 in Salt Stress.

12-oxo-phytodienoic acid reductases (OPRs) have been substantiated as pivotal in plant growth and response to biotic and abiotic stresses. However, the functional characterization of OPR genes in the peanut genome remains limited. In this study, we identified a total of 20 OPR genes in a tetraploid cultivar and two diploid peanut species, categorizing them into two subfamilies, OPRI and OPRII. The gene structure and conserved protein motifs within each subfamily were elucidated. Additionally, our findings indicate an uneven chromosomal distribution of peanut OPR genes. Gene duplication events were identified as pivotal in the expansion of the OPR gene family. An analysis of cis-acting elements within OPR gene promoters revealed the presence of numerous phytohormone- and stress-related cis-elements. Furthermore, peanut OPR genes exhibited tissue-specific and stress-inducible expression patterns, underscoring their crucial role in peanut growth and stress response. Additionally, plants overexpressing AhOPR6 exhibited significantly enhanced resistance to salt stress, and the AhOPR6-OE lines demonstrated a higher ability to scavenge reactive oxygen species (ROS). Collectively, these findings offer deeper insights into the roles of peanut OPR genes in stress responses, suggesting that AhOPR6 could serve as a potential candidate gene for improving peanut salt tolerance through genetic transformation.

Functional analysis of promoter element 2 within the viral polymerase gene of an emerging paramyxovirus, Sosuga virus.

Paramyxovirus genomes carry bipartite promoters at the 3' ends of both their genome and antigenome, thereby initiating RNA synthesis, which requires the viral polymerase to recognize two elements: the primary promoter element 1 (PE1) and the secondary promoter element 2 (PE2). We have previously shown that the antigenomic PE2 (agPE2) in many viruses in the Rubulavirinae subfamily is located within the coding region of the viral RNA polymerase L gene. Sosuga virus (SOSV), belonging to the Rubulavirinae subfamily, is highly pathogenic to humans, thus necessitating high-level containment facilities for infectious virus research. The use of a minigenome system permits studies of viral RNA synthesis at lower biosafety levels. Because minigenomes of negative-strand RNA viruses generally comprise only the untranslated regions, agPE2 within the L coding region-such as those found in Rubulavirinae like SOSV-is typically omitted. However, generating an SOSV minigenome that retains agPE2 led to a pronounced increase in activity, enabling a detailed examination of the role of agPE2 in SOSV replication. In many Rubulavirinae, the agPE2 not only acts as a promoter but also encodes part of the L protein, resulting in a distinct motif at the C-terminus of the L protein. We have further shown that this motif is preserved even in Rubulavirinae that no longer contain the agPE2 within the L gene.IMPORTANCEParamyxoviruses are classified into three major subfamilies: Orthoparamyxovirinae, Avulavirinae, and Rubulavirinae. All paramyxovirus genomes and antigenomes possess bipartite promoters, comprising two elements: promoter element 1 (PE1) at the 3' end and promoter element 2 (PE2) located internally. We previously revealed that, in many Rubulavirinae, the antigenomic PE2 lies within the coding region of the viral RNA polymerase L gene. In this study, we used Sosuga virus, a member of the Rubulavirinae subfamily, to elucidate the role of antigenomic PE2 in viral replication. Because the PE2 region encodes part of the L protein, its presence leads to a distinctive motif at the C-terminus of L protein. Notably, this motif is conserved in all Rubulavirinae, including those that do not harbor the antigenomic PE2 within their L gene, indicating its importance in viral propagation.

Mineralogical and Geochemical Compositions of Sedimentary Rocks in the Gosau Group (Late Cretaceous), Grünbach–Neue Welt Area, Austria

Sedimentary rocks of the Gosau Group in the Grünbach–Neue Welt area (Eastern Alps, Austria) were analyzed to determine their mineralogical and geochemical compositions. This study includes the following: (1) the identification of major minerals using X-ray diffraction (XRD), (2) the analysis of major, minor, and trace elements via X-ray fluorescence spectroscopy (XRF) and inductively coupled plasma mass spectrometry (ICP-MS), and (3) the quantification of total organic carbon (TOC), total nitrogen (TN), and total sulfur (TS) using an Elementar Unicube analyzer. Samples were collected from four artificial trenches and one outcrop in Maiersdorf, spanning the Grünbach and Piesting formations deposited during a terrestrial-to-marine transition in the upper Santonian to Campanian (Late Cretaceous). The dominant minerals—quartz, muscovite, illite, and calcite—exhibit relative abundances corresponding with variations in major oxide concentrations. Minor elements show variability but generally follow consistent trends. Trace and rare earth elements display greater variability but similar patterns, with a broader distribution in the Grünbach Formation. Elevated TOC, TN, and TS values are observed near the formation boundary and in the Piesting formation. These results offer the mineralogical and geochemical characterization of the strata, and lay a foundation for further investigations into the paleoenvironmental and basin evolution of the Gosau Group in the region, providing a comparative framework for Gosau basins across the Eastern Alps.

Source Lithology Characteristics of Different Tectonic Stages of Mariana Subduction Zone: Insights From Major and Trace Element Compositions of Olivine From Basalts

ABSTRACTThe mantle beneath the Mariana subduction zone is commonly described as depleted, primarily composed of peridotite. This study aims to investigate whether subduction processes influence the lithologic characteristics of the mantle source beneath the Mariana subduction zone. We conducted a comprehensive analysis of olivine elemental compositions from basalts in different subduction settings in the Mariana region, including the island arc, back‐arc and initial arc rifting stages. Analysis of major and trace elements in olivines, as well as key elemental ratios (e.g., 10,000 × Zn/Fe, Mn/Zn and 100 × Mn/Fe), reveals that olivines from different subduction settings exhibit characteristics consistent with a peridotite source. While Ca content varies significantly among samples, olivines with high Fo values (Fo > 87) and high Ca contents are consistent with peridotite characteristics. In contrast, olivines with low Fo values (Fo < 87) exhibit lower Ca contents, reflecting the characteristics of a pyroxenite mantle source. This discrepancy is attributed to slab subduction, which increases water content in the mantle wedge and facilitates greater incorporation of Ca into the melt. The compositional distributions of Mn in olivines indicate distinct partitioning behaviours under varying pressure and temperature conditions, with Mn concentrations being particularly sensitive to pressure variations. Therefore, the variation in Mn composition reflects the different pressure conditions experienced by peridotite during its evolution. In different tectonic settings, the variation in Ni contents is mainly controlled by magmatic evolution, with minimal influence from temperature and pressure. Despite the differences in elemental content, our results demonstrate that the mantle source region in the Mariana subduction zone, whether in the island arc, back‐arc basin or initial arc rifting, consistently consists of peridotite, with no different results arising from variations in the subduction processes.

Genome-Wide Characterization and Analysis of the FH Gene Family in Medicago truncatula Under Abiotic Stresses.

The formin family proteins play an important role in guiding the assembly and nucleation of linear actin and can promote the formation of actin filaments independently of the Arp2/3 complex. As a key protein that regulates the cytoskeleton and cell morphological structure, the formin gene family has been widely studied in plants such as Arabidopsis thaliana and rice. In this study, we conducted comprehensive analyses, including phylogenetic tree construction, conserved motif identification, co-expression network analysis, and transcriptome data mining. A total of 18 MtFH gene family members were identified, and the distribution of these genes on chromosomes was not uniform. The phylogenetic tree divided the FH proteins of the four species into two major subgroups (Clade I and Clade II). Notably, Medicago truncatula and soybean exhibited closer phylogenetic relationships. The analysis of cis-acting elements revealed the potential regulatory role of the MtFH gene in light response, hormone response, and stress response. GO enrichment analysis again demonstrated the importance of FH for reactions such as actin nucleation. Expression profiling revealed that MtFH genes displayed significant transcriptional responsiveness to cold, drought, and salt stress conditions. And there was a temporal complementary relationship between the expression of some genes under stress. The protein interaction network indicated an interaction relationship between MtFH protein and profilin, etc. In addition, 22 miRNAs were screened as potential regulators of the MtFH gene at the post-transcriptional level. In general, this study provides a basis for deepening the understanding of the physiological function of the MtFH gene and provides a reference gene for stress resistance breeding in agricultural production.