Amoxicillin Trihydrate Research Articles

Promising sub-100 nm tailor made hollow chitosan/poly(acrylic acid) nanocapsules for antibiotic therapy

Herein, we report on the preparation of ultra-low sized (<100 nm in diameter) biodegradable polymeric capsules for potential applications as nanocontainers in antibiotic therapy. Hollow nanospheres based on the chitosan/poly(acrylic acid) pair are elaborated via (i) the layer-by-layer technique using gold nanoparticles (20 and 60 nm in size) as sacrificial templates, (ii) loading with amoxicillin, a betalactam antibiotic, and (iii) removal of the gold core via cyanide-assisted hydrolysis. Size, dispersity and concentration of the resulting nanocapsules are easily tuned by the nanoparticle templates, while wall thickness is controlled by the number of polyelectrolyte bilayers. Electrostatic interactions between the protonated amine groups of chitosan and the carboxyl groups of poly(acrylic acid) act as the driving attraction force allowing easy and fast design of robust and well-ordered multilayer films. Successful hydrolysis of the gold core is evidenced by time-dependent monitoring of the gold spectroscopic signature (absorbance at 519 nm and 539 nm for the gold nanoparticles with 20 and 60 nm, respectively). Crosslinked capsules are also prepared through crosslinking of the chitosan chains with glutaraldehyde. Chitosan-based nanocapsules are finally evidenced to be promising drug delivery vehicles of amoxicillin trihydrate with tuneable properties such as entrapment efficiency in the range of 62–75% and 3.5–5.5% concerning the drug loading.

In vitro evaluation of the safety and probiotic and technological potential of Pediococcus pentosaceus isolated from sheep milk

Six isolates (Ac1Pd, Ac3Pd, Ac4Pd, Ac5Pd, Ac7Pd, and Ac22Pd) of Pediococcus pentosaceus from sheep milk were tested for safety and for probiotic and technological potential. The results showed that none of the isolates were able to produce biogenic amines or virulence factors. The isolates tested showed low hydrophobicities, high auto-aggregation capacities and co-aggregation with L. monocytogenes ATCC 7644, L. sakei ATCC 15521 and E. faecalis ATCC 19444, but none produced ?-galactosidase and bacteriocins. The isolates did not show growth at pH values 3 and 12, while in a pH range from 4 to 10 the growth was variable. In the absence of bile, all the isolates showed growth, with suppression at bile concentrations of 0.1%, 0.3%, 0.6% and 1.0%. In the disc-diffusion test, the isolates tested were resistant to oxacillin, sulfatrimethoprim and vancomycin but were sensitive to chloramphenicol and tetracycline. The isolates showed variable responses to penicillin G and were resistant to most of the drugs tested, except for amoxicillin trihydrate and ibuprofen. All cultures showed a high milk-acidification capacity after 24 hours and none produced exopolysaccharides. The isolates of P. pentosaceus were able to produce diacetyl; however, no culture showed extracellular proteolytic activity and the autolysis varied from 21.3% to 30.5% after 24 h. The isolates grew at NaCl concentrations of 4.0 and 6.0%, but the growth was lower at 10.0%. Finally, all the isolates were found to be safe but had limited application as probiotics and in some technological uses.

ESI-LC/MS Method Development and Validation for the Determination of Some Selected Antibiotics in Hospital Wastewater

For the last decade, significant attention has been paid to the occurrence, bioaccumulation and fate of drugs in effluent hospital water. Therefore, the aim of this study was to develop and validate analytical method to identify and quantify the antibiotics tetracycline HCl, (Tetra) doxycycline, (Doxy), ampicillin trihydrate (Ampi), amoxicillin trihydrate (Amoxi) and cephalexin monohydrate (Cefalex). The LCMS instrument used was equipped with with C18 column, (150 mm length x 4.6 mm inner diameter x5 um particle size). The mobile phase was acetonitrile/formic acid (1%) under gradient elution mode. The MS employs ESI unit and quadrupole mass analyzer. The analysis time was less than 15 min. The method was validated in terms of linearity, precision, accuracy, robustness, limit of detection and limit of quantitation, specificity, stability and excellent results were obtained.

Validation of HPLC-UV method for determination of amoxicillin Trihydrate in capsule

Validation of HPLC-UV method for determination of amoxicillin Trihydrate in capsule

Electrospun polycaprolactone nanofibres decorated by drug loaded chitosan nano-reservoirs for antibacterial treatments

The main limitation of conventional antibiotic therapies concerns the low efficacy to fight bacteria attacks during long treatment times. In this context, the integrated use of electrofluidodynamics (EFDs)—basically electrospinning and electrospraying—may represent an interesting route for designing nanostructured platforms with controlled release to prevent the formation of bacterial biofilms in oral implant sites. They allow for the deposition of nanofibres and nanoparticles by different modes—i.e. sequential, simultaneous—for the fabrication of more efficacious systems in terms of degradation protection, pharmacokinetic control and drug distribution to the surrounding tissues. Herein, we will investigate EFDs processing modes and conditions to decorate polycaprolactone nanofibres surfaces by chitosan nano-reservoirs for the administration of Amoxicillin Trihydrate as an innovative antibacterial treatment of the periodontal pocket.

Synthesis and characterisation of CU (II) schiff base complex derived from Amoxicillin Trihydrate and Vanillin for antimicrobial application

Synthesis and characterisation of CU (II) schiff base complex derived from Amoxicillin Trihydrate and Vanillin for antimicrobial application

Chitosan Ascorbate Nanoparticles for the Vaginal Delivery of Antibiotic Drugs in Atrophic Vaginitis.

The aim of the present work was the development of chitosan ascorbate nanoparticles (CSA NPs) loaded into a fast-dissolving matrix for the delivery of antibiotic drugs in the treatment of atrophic vaginitis. CSA NPs loaded with amoxicillin trihydrate (AX) were obtained by ionotropic gelation in the presence of pentasodium tripolyphosphate (TPP). Different CSA:TPP and CSA:AX weight ratios were considered and their influence on the particle size, polydispersion index and production yield were investigated. CSA NPs were characterized for mucoadhesive, wound healing and antimicrobial properties. Subsequently, CSA NPs were loaded in polymeric matrices, whose composition was optimized using a DoE (Design of Experiments) approach (simplex centroid design). Matrices were obtained by freeze-drying aqueous solutions of three hydrophilic excipients, polyvinylpirrolidone, mannitol and glycin. They should possess a mechanical resistance suitable for the administration into the vaginal cavity and should readily dissolve in the vaginal fluid. In addition to antioxidant properties, due to the presence of ascorbic acid, CSA NPs showed in vitro mucoadhesive, wound healing and antimicrobial properties. In particular, nanoparticles were characterized by an improved antimicrobial activity with respect to a chitosan solution, prepared at the same concentration. The optimized matrix was characterized by mechanical resistance and by the fast release in simulated vaginal fluid of nanoparticles characterized by unchanged size.

Effects of pore forming agents on chitosan-graft-poly(N-vinylpyrrolidone) hydrogel properties for use as a matrix for floating drug delivery

Eradicating H. pylori-based infection by using conventional oral dosage form of amoxicillin trihydrate finds difficulties to overcome rapid gastric retention time. Encapsulating amoxicillin trihydrate in floating drug delivery system may solve the problem. In this research, the floating drug delivery system of amoxicillin trihydrate encapsulated in floating chitosan-graft-poly(N-vinyl pyrrolidone) hydrogels containing CaCO3 and NaHCO3 as pore forming agents has been successfully prepared. Pore forming agents used was varied with the ratio of 10 to 25% pore forming agents to total mass of the used materials. The hydrogel were characterizedusing FTIR spectrophotometer and stereo microscope. As pore forming agents compositions increased, the porosity (%) and floating properties increased but followed by decrease in drug entrapment efficiency. Most of the floating hydrogels possessed floating ability longer than 180 min and the highest porosity was found in hydrogel containing 25% NaHCO3. Hydrogel containing CaCO3 showed sustained drug release profile than hydrogel containing NaHCO3. However, the optimum formulation was achieved at composition of 10% NaHCO3 with 57% of drug entrapped within the hydrogel and 43% drug released. The results of these studies show that NaHCO3 is an effective pore forming agents for chitosan-graft-poly(N-vinyl pyrrolidone) hydrogel preparation as compare to CaCO3.

Quality Attributes and In Vitro Bioequivalence of Different Brands of Amoxicillin Trihydrate Tablets.

Bacterial resistance and antibiotic drug effectiveness can be related to administering generic products with a subtherapeutic dose or poor in vivo drug release. The aim of this study was to investigate whether locally marketed amoxicillin tablets have the required chemical and physical attributes, including in vitro bioequivalence performance. Five generic products (T1, T2, T3, T4, and T5) containing combination of amoxicillin trihydrate and potassium clavulanate as 1 g strength present in immediate release tablets were compared to the reference listed drug product Augmentin® (R) for weight variation, friability, resistance to crushing, and chemical content of amoxicillin. Difference (f1) and similarity (f2) factors were calculated to assess in vitro bioequivalence requirements. The tablets from different products have shown compliance with the pharmacopeial requirements of the performed tests. The measured resistance to crushing of tablets did not influence the dissolution time. Three generic products released more than 85% of amoxicillin by the first 15 min as did the reference product and were considered as bioequivalent products. T1 and T4 had f1 values of 16.5% and 25.4% respectively and their f2 values were 44.5 and 34.6 respectively, indicating failure to meet in vitro bioequivalence requirements. Tablet formulations can play an important role in achieving bioequivalence. Independent investigations such as this study serve as an important tool to reveal possible inferior or noncompliant products that may find their way to the market.

Biowaiver Monograph for Immediate-Release Solid Oral Dosage Forms: Amoxicillin Trihydrate

Literature and experimental data relevant to waiver of in vivo bioequivalence (BE) testing for the approval of immediate-release solid oral dosage forms containing amoxicillin trihydrate are reviewed. Solubility and permeability characteristics according to the Biopharmaceutics Classification System (BCS), therapeutic uses, therapeutic index, excipient interactions, as well as dissolution and BE and bioavailability studies were taken into consideration. Solubility and permeability studies indicate that amoxicillin doses up to 875 mg belong to BCS class I, whereas 1000 mg belongs to BCS class II and doses of more than 1000 mg belong to BCS class IV. Considering all aspects, the biowaiver procedure can be recommended for solid oral products of amoxicillin trihydrate immediate-release preparations containing amoxicillin as the single active pharmaceutical ingredient at dose strengths of 875 mg or less, provided (a) only the excipients listed in this monograph are used, and only in their usual amounts, (b) the biowaiver study is performed according to the World Health Organization–, U.S. Food and Drug Administration–, or European Medicines Agency–recommended method using the innovator as the comparator, and (c) results comply with criteria for “very rapidly dissolving” or “similarly rapidly dissolving.” Products containing other excipients and those containing more than 875 mg amoxicillin per unit should be subjected to an in vivo BE study.

The effects of amoxicillin with or without clavulanic acid on the postoperative complaints after third molar surgery: a retrospective chart analysis.

The aim of this chart-based retrospective study was to evaluate the effects of orally administered amoxicillin alone or amoxicillin combined with clavulanic acid on the frequency of post-operative complications and patients' comfort after mandibular third molar surgery. The records of patients who had undergone lower third molar surgery between October 2014 and December 2015 were examined. A total of 62 patients who had fully impacted teeth in mesioangular position and who had been prescribed same type and dose of anti-inflammatory drug were included in this study. Among them, 32 subjects were found to have been prescribed 500 mg amoxicillin trihydrate orally every 8 h for 5 days (Group A) and 30 patients 500 mg amoxicillin trihydrate plus 125 mg potassium clavulanate orally every 8 h for 5 days postoperatively (Group AC). Post-operative pain levels, swelling, presence of trismus, frequency of alveolar osteitis and quality of life (QoL) scores were gathered from patients' charts and were statistically compared. Analysis of the variables showed that there were no significant differences between the Groups A and AC regarding pain levels, swelling, trismus and QoL scores. The frequency of alveolar osteitis was found to be 1.6% in the Group A, however, no significant difference was observed among study groups. Within the limitations of this retrospective chart review, it can be stated that amoxicillin and amoxicillin with clavulanic acid might provide similar outcome in terms of patient comfort following third molar surgery.

Spectroscopic Study for Determination of Amoxicillin Using Cobalt(II) as Complexing Metal

This study includes analytical methods for the determination of the drug amoxicillin trihydrate (Amox.) in some pharmaceutical preparations using Cobalt ion (Co(II)) as complexing metal. The best conditions for complexation were: the reaction time was 20 minutes, pH=1.5 and the best temperature of reaction was 70 ËšC. Benzyl alcohol was the best solvent for extraction the complex.&#x0D; Keywords: Amoxicillin, Cobalt(II), Complex, Molar ratio.

Validated electrochemical and chromatographic quantifications of some antibiotic residues in pharmaceutical industrial waste water.

Realistic implementation of ion selective electrodes (ISEs) into environmental monitoring programs has always been a challenging task. This could be largely attributed to difficulties in validation of ISE assay results. In this study, the electrochemical response of amoxicillin trihydrate (AMX), ciprofloxacin hydrochloride (CPLX), trimethoprim (TMP), and norfloxacin (NFLX) was studied by the fabrication of sensitive membrane electrodes belonging to two types of ISEs, which are polyvinyl chloride (PVC) membrane electrodes and glassy carbon (GC) electrodes. Linear response for the membrane electrodes was in the concentration range of 10-5-10-2mol/L. For the PVC membrane electrodes, Nernstian slopes of 55.1, 56.5, 56.5, and 54.0mV/decade were achieved over a pH 4-8 for AMX, CPLX, and NFLX, respectively, and pH 3-6 for TMP. On the other hand, for GC electrodes, Nernstian slopes of 59.1, 58.2, 57.0, and 58.2mV/decade were achieved over pH 4-8 for AMX, CPLX, and NFLX, respectively, and pH 3-6 for TMP. In addition to assay validation to international industry standards, the fabricated electrodes were also cross-validated relative to conventional separation techniques; high performance liquid chromatography (HPLC), and thin layer chromatography (TLC)-densitometry. The HPLC assay was applied in concentration range of 0.5-10.0μg/mL, for all target analytes. The TLC-densitometry was adopted over a concentration range of 0.3-1.0μg/band, for AMX, and 0.1-0.9μg/band, for CPLX, NFLX, and TMP. The proposed techniques were successfully applied for quantification of the selected drugs either in pure form or waste water samples obtained from pharmaceutical plants. The actual waste water samples were subjected to solid phase extraction (SPE) for pretreatment prior to the application of chromatographic techniques (HPLC and TLC-densitometry). On the other hand, the fabricated electrodes were successfully applied for quantification of the antibiotic residues in actual waste water samples without any pretreatment. This finding assures the suitability of the fabricated ISEs for environmental analysis.

Metal Complexes of a Novel Schiff Base Based on Penicillin: Characterization, Molecular Modeling, and Antibacterial Activity Study.

A novel Schiff base ligand of type HL was prepared by the condensation of amoxicillin trihydrate and nicotinaldehyde. The metal complexes of Co+2, Ni+2, Cu+2, and Zn+2 were characterized and investigated by physical and spectral techniques, namely, elemental analysis, melting point, conductivity, 1H NMR, IR, UV-Vis spectra, ESR, SEM, and mass spectrometry measurements. They were further analyzed by thermal technique (TGA/DTA) to gain better insight about the thermal stability and kinetic properties of the complexes. Thermal data revealed high thermal stability and nonspontaneous nature of the decomposition steps. The Coats-Redfern method was applied to extract thermodynamic parameters to explain the kinetic behavior. The molar conductance values were relatively low, showing their nonelectrolytic nature. The powder XRD pattern revealed amorphous nature except copper complex (1c) that crystallized in the triclinic crystal system. The EPR study strongly recommends the tetrahedral geometry of 1c. The structure optimization by MM force field calculation through ArgusLab 4.0.1 software program supports the concerned geometry of the complexes. The in vitro antibacterial activity of all the compounds, at their two different concentrations, was screened against four bacterial pathogens, namely, E. coli, P. vulgaris, K. pneumoniae, and S. aureus, and showed better activity compared to parent drug and control drug.

Derivative Spectrophotometric Estimation of Amoxicillin Trihydrate and Carbocisteine by Third Order Derivative Spectroscopy method in Combined Dosage Form

In the proposed method UV spectrophotometric, third order derivative method was developed for validation of amoxicillin tri-hydrate and carbocisteine from pharmaceutical formulation. The ICH guidelines were used for validation of amoxicillin and carbocisteine. The derivative method involves the measurement of absorbance, hence wavelengths 236.2 nm and 210 nm were found to be suitable for the estimation of amoxicillin and carbocisteine respectively. The linearity was found in the concentration range of 10 to 100 µg /ml for amoxicillin and 1 to 10 µg /ml for carbocisteine respectively. For amoxicillin the percentage mean recovery was found to be 100.075% and for carbocisteine was 100.031 % respectively. The linearity, accuracy and precision of method was validated by statistically. The less percentage (%) RSD values indicates both intra and inter day have high grade of precision.

The Quality of Selected Essential Medicines Sold in Accredited Drug Dispensing Outlets and Pharmacies in Tanzania.

IntroductionThe purpose of this study was to investigate the quality of a select group of medicines sold in accredited drug dispensing outlets (ADDOs) and pharmacies in different regions of Tanzania as part of an in-depth cross-sectional assessment of community access to medicines and community use of medicines.MethodsWe collected 242 samples of amoxicillin trihydrate, artemether-lumefantrine (ALu), co-trimoxazole, ergometrine maleate, paracetamol, and quinine from selected ADDOs and pharmacies in Mbeya, Morogoro, Singida, and Tanga regions. The analysis included physical examination and testing with validated analytical techniques. Assays for eight of nine products were conducted using high-performance thin-layer chromatography (HPTLC). For ALu tablets, we used a two-tiered approach, where tier 1 was a semi-quantitative Global Pharma Health Fund-Minilab® method and tier 2 was high-performance liquid chromatography (HPLC) as described in The International Pharmacopoeia’s monograph for artemether-lumefantrine.Results and DiscussionThe physical examination of samples revealed no defects in the solid and oral liquid dosage forms, but unusual discoloration in an injectable solution, ergometrine maleate. For ALu, the results showed that of 38 samples, 31 (81.6%) passed tier 1 testing and 7 (18.4%) gave inconclusive drug content results. The inconclusive ALu samples were submitted for tier 2 testing and all met the quality standards. The pass rate using the HPTLC and TLC/HPLC assays was 93.8%; the failures were the ergometrine maleate samples purchased from both ADDOs and pharmacies. The disintegration testing of the solid dosage forms was conducted in accordance with US Pharmacopeia monographs. Only two samples of paracetamol, 1.2% of the solid dosage forms, failed to comply to standards. The study revealed a high overall rate of 92.6% of samples that met the quality standards. Although the overall failure rate was 7.4%, it is important to note that this was largely limited to one product and likely due to poor distribution and storage rather than poor manufacturing practices.ConclusionsOver 90% of the medicines sold in ADDOs and pharmacies met quality standards. Policy makers need to reconsider ergometrine maleate’s place on the list of medicines that ADDOs are allowed to dispense, by either substituting a more temperature-stable therapeutically equivalent product or requiring those sites to have refrigerators, which is not a feasible option for rural Tanzania.

Hydrogels based on starch‐g‐poly(sodium‐2‐acrylamido‐2‐methyl‐1‐propane sulfonate‐co‐methacrylic acid) as controlled drug delivery systems

Novel hydrogel systems based on starch‐g‐poly(sodium‐2‐acrylamido‐2‐methyl‐1‐propane sulfonate‐co‐methacrylic acid) [starch‐g‐poly(AMPSNa‐co‐MAA)] has been prepared through graft copolymerization of AMPSNa and MAA onto starch chains. Characterization of these hydrogels is carried out using Fourier transform infrared (FT‐IR) spectroscopy and scanning electron microscopy (SEM). Hydrogels with maximum swelling capacity have exhibited better drug absorption suggesting that they are suitable as carriers for controlled drug delivery. The capability of these hydrogels as drug delivery vehicles has been evaluated at pH values 4.0, 7.0, and 9.2 using amoxicillin trihydrate as the model drug and these hydrogels have shown more drug release in basic medium when compared to acidic or neutral media.

Fabrication & Characterization of 3D Electrospun Biodegradable Nanofibers for Wound Dressing, Drug Delivery and Other Tissue Engineering Applications.

The use of electrospinning technology (ET) in fabrication of threedimensional biodegradable electrospun nanofibers scaffolds (BENS) has recently gained considerable attention in tissue engineering. BENS are superior to other existing scaffolds in tissue regeneration as they provide high surface area-to-volume ratio, possess high porosity, and offer a biomimetic environment in a nanometer scale. To fabricate & characterize BENS using Poly (ethylene glycol) (PEG35000) as a biodegradable polymer loaded with Amoxicillin Trihydrate (AMX) for use as a wound dressing. Solutions of PEG35000 in chloroform of varying concentrations were used to fabricate BENS using ET. Blank & 1% w/v AMX-loaded BENS were fabricated & characterized. Morphology of BENS were assessed using Scanning Electron Microscopy (SEM). Fourier Transform Infrared (FT-IR) Spectroscopy was used to identify the interaction between PEG35000 and AMX. Differential Scanning Calorimetry (DSC) was used to assess the crystallinity and thermal behavior of the prepared BENS. The X-Ray Diffraction (XRD) analysis for the blank and drug loaded electrospun fibers was carried out to identify the changes in their crystalline pattern. The in vitro antibacterial activity against common skin Gram-negative and Gram-positive pathogens was also tested. Blank & AMX loaded 35% w/v PEG35000 solutions produced the most homogenous and intact nanofibers. Major bands of AMX in FTIR were clearly observed in the spectrum of AMX with PEG35000 post electrospinning. Moreover, DSC thermograms indicated that AMX existed in its amorphous dispersed state within PEG fibers supported by the disappearance of its melting peak at 190°C and confirmed by the complete absence of AMX crystals under SEM. Finally, the results of DSC were confirmed by XRD patterns. Characterizing XRD peaks of AMX loaded with PEG3500 post electrospinning disappeared as an indication of the complete dispersion of AMX in the loaded fibers and its complete conversion to the amorphous form. The in vitro antibacterial assay confirmed the efficiency of the drug loaded fibers against the common skin pathogens. BENS using PEG35000 loaded with AMX were successfully fabricated and characterized. Our findings show that PEG BENS has features that make it a promising candidate for wound healing applications.

EFFICACY OF OXYTETRACYCLINE, AMOXICILLIN, SULFAMETHOXAZOLE AND TRIMETHOPRIM, AND TYLOSIN FOR THE TREATMENT OF BACTERIAL DISEASES IN CATTLE AND GOATS

A single blind clinical trial was carried out in Satkhira district where dairy operations by Community-based Dairy Veterinary Foundation,BangladeshAgriculturalUniversity, Mymensingh were running. A total of 80 cattle and goats affected with different bacterial diseases were used for treatment with oxytetracycline, amoxicillin, combined preparation of sulfamethoxazole and trimethoprim, and tylosin. Diagnosis of diseases was based on clinical history and signs. Haemorrhagic septicemia (HS) affected cattle aged between 3 and 6 were treated with oxytetracycline (Tetravet-100,Acme,Bangladesh; DUFA-OTC 20% LA, DutchFarm,Holland). Amoxicillin (Amoxicillin trihydrate, Jickstar Pharma Inc.,Philippines; Moxilin Vet LA, The ACME Laboratories Ltd.,Bangladesh) was used in the treatment of calf pneumonia. Foot rot in cows, arthritis in calf and goat were treated with sulphamethoxazole and trimethoprim (Politrim-Vet, The ACME Laboratories Ltd.,Bangladesh). Bronchopneumonia in non-lactating cows and goats were treated with tylosin (Tylovet (Acme). The efficacy of oxytetracycline, amoxicillin, sulfamethoxazole and trimethoprim, and tylosin for the treatment of haemorrhagic septicemia, calf pneumonia, foot rot and bronchopneumonia were evaluated on the basis of recovery rate. Treatment of haemorrhagic septicemia with both antibiotics showed 100% recovery rate. The duration of complete recovery in Tetravet-100 treated group was 4-6 days with median of 5 days. In the DUFA-OTC 20% LA treated group it was 3-5 days with median of 4 days. Moxilin Vet® (Acme) and Amoxicillin trihydrate® (Jickstar Pharma) were evaluated against calf pneumonia in cattle. Cent percent cattle were cured with both the antibiotics, Moxilin Vet® (Acme) and Amoxicillin trihydrate® (Jickstar Pharma). For both antibiotics, the median days of complete recovery was 5. Of 5 foot rot affected cows treated with a combined preparation of sulfamethoxazole and trimethoprim (Politrim-Vet®, Acme), 4 (80%) recovered within 4-5 days of treatment. However, treatment of arthritis affected calf and goat with same preparation showed 90% and 100% recovery rate, respectively. Treatment of bronchopneumonia cases with tylosin (Tylovet®, Acme) resulted in 80% recovery in non-lactating cows and 90% in goats and it took 4-5 days for recovery with a median of 4 days.

Partial Least-Squares and Linear Support Vector Regression Chemometric Methods for Simultaneous Determination of Amoxicillin Trihydrate and Dicloxacillin Sodium in the Presence of Their Common Impurity.

Two multivariate chemometric models, namely, partial least-squares regression (PLSR) and linear support vector regression (SVR), are presented for the analysis of amoxicillin trihydrate and dicloxacillin sodium in the presence of their common impurity (6-aminopenicillanic acid) in raw materials and in pharmaceutical dosage form via handling UV spectral data and making a modest comparison between the two models, highlighting the advantages and limitations of each. For optimum analysis, a three-factor, four-level experimental design was established, resulting in a training set of 16 mixtures containing different ratios of interfering species. To validate the prediction ability of the suggested models, an independent test set consisting of eight mixtures was used. The presented results show the ability of the two proposed models to determine the two drugs simultaneously in the presence of small levels of the common impurity with high accuracy and selectivity. The analysis results of the dosage form were statistically compared to a reported HPLC method, with no significant difference regarding accuracy and precision, indicating the ability of the suggested multivariate calibration models to be reliable and suitable for routine analysis of the drug product. Compared to the PLSR model, the SVR model gives more accurate results with a lower prediction error, as well as high generalization ability; however, the PLSR model is easy to handle and fast to optimize.