Amount Of Free Fatty Acids Research Articles

Efficient In Vitro Digestion of Lipids and Proteins in Bovine Milk Fat Globule Membrane (MFGM) Ingredient and Infant Formula Containing MFGM

ObjectivesThe relative immaturity of the infant digestive system after birth has the potential to affect the bioaccessibility of dietary lipids, proteins and their digested product. The in vitro digestion of lipids and protein in bovine milk fat globule membrane (MFGM) ingredient and infant formula without and with MFGM ingredient (bovine source), as well as the partitioning of non-digested and products of digestion in bioaccessible fraction of chyme were measured. Conditions that simulate those in the stomach and small intestine of infants were used.MethodsMFGM ingredient and whey/casein-based infant formula with and without 6%MFGM ingredient were digested using static, two-phase in vitro digestion model with conditions simulating those in the infant gut. Digestion and bioaccessibility of lipids were monitored by measuring a wide lipid profile using direct infusion-mass spectrometry (DI-MS/MS). Digestion of proteins were determined by denaturing polyacrylamide gel electrophoresis, with quantitative imaging of all blue stained bands.ResultsCholesterol esters, diacylglycerides, triacylglycerides, phosphatidylcholines, and phosphatidylethanolamines in the MFGM ingredients were readily hydrolyzed resulting in marked increases in the amounts of free fatty acids and lyso-phospholipids in the bioaccessible fraction of chyme. In contrast, sphingomyelins, ceramides and GM3 gangliosides were relatively resistant to digestion. Protein in MFGM ingredient and infant formula without and with added MFGM ingredient also were efficiently digested.ConclusionsThe results suggest proteins, neutral lipids and phospholipids in MFGM are efficiently digested using conditions that simulate those in the prandial lumen of the stomach and small intestine of infants. Also, the profile of lipid and protein digestion is similar for whey/casein-based infant formula without and with MFGM ingredient using conditions that simulate those in the infant gut.Funding SourcesOhio State Agriculture Research and Development Center, the JT Parker Endowment and Reckitt Nutrition Institute.

Evaluation and kinetic study of alkaline ionic liquid for biodiesel production through transesterification of sunflower oil

Biodiesel production is performed in the industry by alkaline transesterification of oils with a low amount of free fatty acids. In order to reduce the disposal of conventional catalysts used industrially, ionic liquids (ILs) have been studied to be applied as catalysts in transesterification since they can be recovered and reused in subsequent reaction cycles. In this work, the ionic liquid choline hydroxide (ChOH) was successfully applied as a catalyst for the transesterification reaction of triacylglycerols present in sunflower oil with methanol. A kinetic modeling study under the specific conditions of 2 wt% catalyst dosage, 1:10 oil/methanol molar ratio, for 0–120 min at 35–65 ℃ was conducted, and liquid–liquid extraction with water/butanol was evaluated as a process to recover the IL. A 95.0% ester yield content was achieved in this work for a short reaction time (30 min). Furthermore, the results of the kinetic study demonstrated that a first-order model was the best fit for the reaction with a rate constant (k) estimated as 0.1182 min−1 and activation energy (Ea) of 13.64 kJ/mol. For the tested conditions, the complete recovery of the IL using liquid–liquid extraction did not occur since it is noted the presence of ChOH in both phases.

Cholecalciferol- and α-tocopherol-loaded walnut oil emulsions stabilized by whey protein isolate and soy lecithin for food applications.

To overcome the limitations in the use of protein as an emulsifier, soy lecithin, a natural surfactant, was used along with whey protein isolate (WPI) to produce o/w emulsions containing cholecalciferol and α-tocopherol. The physical stability of the emulsions prepared with WPI and varying concentrations of lecithin (0, 1, 2, and 3% w/w) was measured in different heat, pH, and ionic-strength food environmental conditions. All emulsions were shown to be less than 250 nm in size and less than 0.3 in polydispersity index (PDI). The morphology of the emulsions was spherical, and the droplets of the emulsion containing lecithin were thicker and larger than those of the emulsion without lecithin (WPI_L0). After autoclaving, WPI_L0 increased in size from 197.8 ± 1.7nm to 528.5 ± 28.4nm, and the retention of cholecalciferol and α-tocopherol decreased to 40.83 ± 0.63% and 49.68 ± 1.84%, respectively. At pH5.5, near the isoelectric point of WPI, WPI_L0 increased in size due to aggregation, but emulsions containing lecithin remained stable at a PDI under 0.3. Turbiscan stability index of the emulsion prepared with WPI and 3% lecithin was the lowest, indicating good storage stability. In addition, it was confirmed that the higher the lecithin content, the higher the viscosity, and the higher the amount of free fatty acids released in the in vitro digestion model. This study can provide theoretical evidence for enhancing the physical stability of protein emulsions by co-stabilization with lecithin, promoting their application in various foods. © 2022 Society of Chemical Industry.

Biodiesel production by single-step acid-catalysed transesterification of Jatropha oil under microwave heating with modelling and optimisation using response surface methodology

Biodiesel is known as one of the best alternative fuels for diesel engines. Low-cost Jatropha oil is considered a potential non-edible feedstock for biodiesel production in India and many other parts of the world. Jatropha oil contains a large amount of free fatty acids (FFA), and soap formation occurs during the alkali catalysed transesterification process, hence decreasing the biodiesel yield. The acid catalyst is less sensitive to FFA, but the reaction rate is extremely slow if the transesterification reaction occurs by conventional heating. In the present investigation, microwave heating was used for biodiesel production by the single-step transesterification reaction of Jatropha oil in the presence of an acidic catalyst (sulphuric acid). The central composite rotatable design (CCRD) matrix of response surface methodology (RSM) was employed to determine the optimum design conditions for the transesterification reaction under microwave irradiation. The effects of three selected variables, namely reaction time, catalyst concentration, and methanol, on the oil molar ratio, were assessed. The maximum yield of biodiesel produced in the selected design space by microwave heating was found to be 61.10% under the 11:7 M ratio of the methanol to oil, 2 wt% catalyst concentration, and 90 min reaction time, which was much higher than the biodiesel yield by conventional heating method (3.8%) for the same reaction time. The modified polynomial model for the microwave heating method was developed with the help of ANOVA, main effect plots, interaction plots, and surface plots. The experimental and predicted yield values for fatty acid methyl ester (FAME) showed a linear relationship. The validation of experiments confirmed the accuracy of the suggested model. The produced biodiesel was of good quality, as all the properties were within the prescribed limits of the ASTM D6751 standard. The results of this study showed that the microwave heating method can be used efficiently to obtain a high biodiesel yield from low-cost, high-FFA feedstock such as Jatropha oil in a sulphuric acid-catalysed single-step transesterification reaction.

Saurani Zeytinyağinin Bazi Kalite Parametreleri Üzerine Zeytin Olgunluğunun Etkisi

There are 97 local olive varieties registered in our country, and Saurani is an olive variety from originating Hatay/Altınözü. Hatay province, which ranks first in olive production in the Mediterranean Region, has very suitable climate and soil conditions for olive cultivation. The aim of this study is to investigate the effect of olive maturity on quality parameters such as free fatty acids, peroxide value and fatty acid composition of the oil obtained from the Saurani olive variety grown in Hatay. For this purpose, olive oil was obtained by mechanical method from olives collected from Saurani variety in 3 different maturity periods of the 2020 production season. In Saurani olive oil, free fatty acids and peroxide numbers were found in the range of 0.62-0.91 % (oleic acid) and 3.68-5.26 meq O2/kg respectively. The amount of free fatty acids increased with maturity. In Saurani olive oil were determined oleic acid in the range of 66.32%-68.79%, palmitic acid in the range of12.47-13.75%, linoleic acid in the range of 11.43-13.84%, stearic acid in the range of 3.16-3.42%, palmitoleic acid 1.12%-1.34%, linolenic acid 0.88-1.01% and arachidic acid 0.41-0.52. It was determined that decrease in oleic acid content and an increase in linoleic acid content with maturity. It has been determined that Saurani olive oil is within the limits specified in the Turkish Food Codex on Olive Oil and Pirina Olive Oil in terms of the examined properties.

Effects of the interaction between bacterial cellulose and soy protein isolate on the oil-water interface on the digestion of the Pickering emulsions

This work was aimed at engineering and modulating oil-in-water (O/W) composite interfaces by controlling the interaction between cellulose and protein to delay the lipid digestion. The insoluble bacterial cellulose nanofibrils/soy protein isolate (BCNFs/SPI) complexes were fabricated at pH 3.0. The interfacial adsorption characteristics of SPI particles were improved in the presence of BCNFs, resulting in the enhanced interfacial pressure and dilatational viscoelastic moduli due to the rigid and compact layers. O/W Pickering emulsions stabilized by BCNFs/SPI complexes were prepared, and the prepared emulsion presented improved stability by tuning BCNFs/SPI ratios. Moreover, the emulsions stabilized by BCNFs/SPI complexes displayed lower amount of free fatty acid (FFA) released than that of SPI stabilized emulsion after in vitro simulated digestion, with the minimum amount of FFA released of 58.0% at the BCNFs/SPI ratio of 1:5. Main mechanisms were proposed for the results: (1) the compact interfacial layers formed by complexes partially restricted interfacial displacement by bile salts or lipases; (2) bridging structure and interconnected networks formed by BCNFs reduced the surface area available for binding with lipases and bile salts. This work would be suggestive for the development of cellulose-protein interface to modulate the digestion of Pickering emulsified lipids.

Antimicrobial Activity by a Unique Composition of Cold Pressed Nigella Sativa Seed (Black Cumin) Oil

The oil produced from Nigella sativa seeds (black cumin) is a known folklore remedy for treatment of various health conditions, including the treatment of microbial infections. The microbes Malassezia furfur, Candida albicans and Staphylococcus aureus are commonly found in healthy skin and gut microbiota, but can also be infection-inducing microorganisms upon overgrowth, capable of disrupting the homeostasis of the microbiome. Treatment with antibiotics has led some of these microbes to evolve antibiotic-resistant strains. Several Nigella sativa oils produced by cold pressing of agricultural crops to contain high or low amounts of the active molecule, thymoquinone as well as high or low amounts of free fatty acids were tested for their antifungal and antibacterial properties. The growth of Malassezia furfur and an antibiotic-resistant strain of Candida albicans were most inhibited by a combination of high amounts of Thymoquinone (3%) and low amounts of free fatty acids (2%). While the growth of Staphylococcus aureus is strongly inhibited by Nigella sativa oil, it seems that another component, yet to be identified, is responsible for the antibacterial effect as all oil compositions tested presented similar and strong inhibition of the bacterial growth. According to the results, Nigella sativa oil may be used as an alternative safe antimicrobial agent, and perhaps even as a preventative care for maintenance of microbiome balance and diversity.

Esterification reaction of free fatty acid in used cooking oil using sulfonated hypercrosslinked exchange resin as catalyst

The production of valuable fatty acid alkyl ester (FAAE) commercially known as biodiesel from used cooking oil (UCO) as feedstock has recently gained global interest. To carry out this production process, an esterification reaction is necessary to minimize the amount of free fatty acid (FFA) in the UCO. The purpose of this research work is to evaluate the performance of the newly snythesized sulfonated hypercrosslinked exchange resin (SHER) for the esterification reaction. The catalyst was first synthesized and characterized using various physicochemical analyses (i.e. Fourier transform infra-red (FTIR), surface morphology, N2 physisorption analysis and thermogravimetric analysis (TGA)) and further subjected to the esterification reaction to determine the reaction kinetics. The esterification reaction was conducted at various catalyst loading (1−8 wt%), reaction temperatures (40−60 °C), and methanol to oil molar ratios (6:1−24:1). From the characterization study, SHER was found to have high decomposition temperature (up to 398 °C) and specific surface area (836 m2 g−1). Additionally, SHER had the capability to accommodate high number of active sites which could benefit the esterification reaction. The highest FFA conversion of 97% was achieved at 5 wt% catalyst loading, 60 °C of reaction temperature and 12:1 methanol to oil mole ratio in 2 h reaction time. Analyses of the spent catalyst revealed the presence of impurities residue on the SHER's surface following the esterification reaction, and the catalyst pore remained evident even after several reusability cycles. Finally, the Eley-Rideal, pseudo-homogeneous and Langmuir-Hinshelwood-Hougen-Watson kinetic models were proposed. Due to its high activation energy; i.e. 48.4 kJ mol−1, the reaction was found to be limited by surface reaction and governed by the chemical step.

A review of sustainable biodiesel production using biomass derived heterogeneous catalysts.

The production of biodiesel through chemical production processes of transesterification reaction depends on suitable catalysts to hasten the chemical reactions. Therefore, the initial selection of catalysts is critical although it is also dependent on the quantity of free fatty acids in a given sample of oil. Earlier forms of biodiesel production processes relied on homogeneous catalysts, which have undesirable effects such as toxicity, high flammability, corrosion, by‐products such as soap and glycerol, and high wastewater. Heterogeneous catalysts overcome most of these problems. Recent developments involve novel approaches using biomass and bio‐waste resource derived heterogeneous catalysts. These catalysts are renewable, non‐toxic, reusable, offer high catalytic activity and stability in both acidic and base conditions, and show high tolerance properties to water. This review work critically reviews biomass‐based heterogeneous catalysts, especially those utilized in sustainable production of biofuel and biodiesel. This review examines the sustainability of these catalysts in literature in terms of small‐scale laboratory and industrial applications in large‐scale biodiesel and biofuel production. Furthermore, this work will critically review natural heterogeneous biomass waste and bio‐waste catalysts in relation to upcoming nanotechnologies. Finally, this work will review the gaps identified in the literature for heterogeneous catalysts derived from biomass and other biocatalysts with a view to identifying future prospects for heterogeneous catalysts.

Co-encapsulation of (–)-epigallocatechin-3-gallate and quercetin in double emulsion hydrogel beads: Microstructures, functional properties, and digestion behaviors

Co-loaded (–)-epigallocatechin-3-gallate (EGCG) and quercetin double emulsions and hydrogel beads were prepared, and their structure, functions, and digestion characteristics were investigated. The double emulsion particles were adsorbed by the cross-linked chains of the hydrogel beads. The encapsulation efficiencies of EGCG and quercetin within the hydrogel beads were higher than those within the double emulsion, while the antioxidant activities of the double emulsions were higher than those of the hydrogel beads. A lower amount of free fatty acids (FFAs) was released from the hydrogel beads than that released from the double emulsions. The bioavailability of EGCG was higher in the hydrogel beads than those in the double emulsions, while the quercetin bioavailability was not significantly different expect for the ratio of 3:7. The hydrogel beads remained intact in the stomach; however, numerous oil spills occurred in the small intestine. These data may improve double-emulsion-based delivery systems for controlled lipolysis and the release of co-encapsulated hydrophilic and lipophilic bioactive compounds.

Statistical Optimisation using Taguchi Method for Transesterification of Reutealis Trisperma Oil to Biodiesel on CaO-ZnO Catalysts

Optimisation of biodiesel production from non-edible Reutealis Trisperma oil (RTO) was investigated using Taguchi method. Biodiesel was produced via consecutive esterification and transesterification reactions. Esterification of RTO was carried out using acid catalyst to decrease the amount of free fatty acid from 2.24% to 0.09%. Subsequent transesterification of the treated oil with methanol over a series of CaO-ZnO catalysts was optimized based on the L9 Taguchi orthogonal approach. The optimization parameters are Ca/Zn ratio (0.25, 0.5, and 1), methanol/oil ratio (10, 20, and 30) and reaction time (0.5, 1, and 2 h). CaO-ZnO catalysts at variation of Ca/Zn ratios were prepared using co-precipitation method and characterized using XRD, SEM, TEM, and FTIR analysis. The amount of methyl ester yield was used as the response parameter in the S/N ratio analysis and Analysis of Variance (ANOVA). The optimum parameter for RTO transesterification to biodiesel was determined at Ca/Zn ratio of 1, methanol oil ratio of 30 and reaction time for 2 h. Transesterification under these optimized parameter generated 98% of biodiesel yield, inferring the validity of the statistical approach. Furthermore, ANOVA analysis also confirmed that all the parameters were significantly contributed at approximately equal percentage towards the amount of biodiesel. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Characterization of Crude Palm Oil (CPO), Corn Oil and Waste Cooking Oil for Biodiesel Production

Biodiesel production is the reaction of raw oils with mixing and heating within catalyst and methanol. The raw oils usually come from vegetable oils and animal fats. Vegetable oils are a promising feedstock for biodiesel production since they are renewable in nature. Nevertheless, the physical properties of biodiesel pose some acute problems when used in an unmodified engine. It is important to diesel and biodiesels because it impacts components such as the fuel pump. Therefore, this paper intends to investigate the properties of biodiesel samples in terms of viscosity, density, flash point and acid values at different bio lipids and different mixing time. The evaluation is carried out on the three types of biodiesels: crude oil, crude palm oil, corn oil, and waste cooking oil. Methanol was chosen over the others for the transesterification process because it was cheaper. The esterification process, which reduces the amount of free fatty acids in the crude oil, will be performed with the help of an acid catalyst. Alkaline catalysts, in contrast, are used for the transesterification process. The comparison of all the samples shows that CPO is the better biodiesel than the other due to the physical properties of kinematic viscosity, density and flashpoint.

Metabolic regulation in mitochondria as a prospective way of body rejuvenation

Scientists around the world are looking for ways to achieve high-quality and active longevity. The complexity of these searches is associated with the lack of unified concept of aging mechanisms, the problem of extrapolating the results of studies of geroprotectors from model organisms to humans. Therefore, modern medicine can only slow down the aging process so far. Nevertheless, it has been proven that disturbances in the functioning of mitochondria are an important link in the pathogenesis of aging; therefore, regulation of their metabolism is a promising way of rejuvenating the body. A decrease in protein synthesis and a general decrease in the intensity of the oxidative enzymes action are interrelated. Energy production for the protein and DNA synthesis decreases with a decrease in the intensity of oxidative processes. Dysfunction of mitochondrial adaptive reactions also develops with age, which may be associated with the low efficiency of the NADH dehydrogenase complex – NADH ubiquinone oxidoreductase, which catalyzes the transfer of electrons from NADH to ubiquinone/coenzyme Q10, resulting in a decrease in electron transport and an increase of active oxygen forms. Coenzyme Q10 reducing plays a very important role in the aging process, as it is an antioxidant and, unlike other antioxidants, is produced in the body. An increase in mitochondrial biogenesis, as well as an increase in the rate and efficiency of mitochondrial metabolism (with a calorie restricted diet for example) helps to improve the energy supply of cells and prolong life.A drug based on xylitol Xylate can be a source of triglycerophosphate by metabolism through the pentose phosphate cycle and thereby reduce the amount of free fatty acids that can be oxidized into acetyl-CoA, increases the content of adenosine triphosphate acid and other adenine nucleotides in liver, and has a pronounced antiketogenic effect. It can reduce intoxication and improve microcirculation, which is especially important for high-quality and active longevity.Activation of mitochondrial metabolism is a promising direction in the aging prevention, since it allows balancing metabolic processes not only in cells, but also in body as a whole.

Impregnated Zeolite as Catalyst in Esterification Treatment from High Free Fatty Acids Palm Oil Mill Effluent

Although Palm Oil Mill Effluent (POME) can be used as a raw material for biodiesel production, the POME contains an excessive amount of Free Fatty Acids (FFA), necessitating a preliminary process and esterification. POME is degummed using phosphate acid and bleached with carbon active. Additionally, this study used KOH-impregnated zeolite to reduce FFA. The purpose of this study is to determine the effect of adding impregnated zeolite on esterification. POME was heated to 600C for 30 minutes, then degumming with 3 % of phosphate acid for 30 minutes, followed by bleaching with carbon active with a comparison ratio of 8:3 at 1000C for 1 hour, and finally, esterification with 3 percent impregnated zeolite from the POME weight at 600C for 4 hours. The analysis was conducted using titration methods to determine the FFA of each esterification. The data will be compared between zeolite and non-zeolite degumming, bleaching, and esterification. The results indicated that the most effective method for reducing FFA was degumming, bleaching, and esterification with zeolite.

Effect of Yarrowia lipolytica RO25 cricket-based hydrolysates on sourdough quality parameters

The principal aim of this research was to use Yarrowia lipolytica RO25 to obtain a cricket powder-based hydrolysate to produce sourdough for bread production. RO25 hydrolysed cricket sourdough (RO25H-CS) was compared with a control traditional sourdough and a control containing the no-hydrolysed cricket powder. Microbiological analyses evidenced a good growth of Yarrowia lipolytica in RO25 cricket hydrolysate and the RO25H-CS was characterized, in comparison with the controls, by a marked and peculiar total protein profile, attributed to the well-known proteolytic activities of Yarrowia lipolytica. RO25H-CS was also endowed with a specific profile in free fatty acids, including arachidonic and linolenic having a functional role. Moreover, RO25H-CS was characterized, compared with the control samples, by the highest releases of C18:2, C18:1 and C16:1, which are considered aroma precursors. In fact, the presence of highest proteolytic activity and the highest amount of free fatty acids detected in RO25H-CS sample underline a specific volatile molecules profile. The results obtained showed the great potential of Yarrowia lipolytica RO25 to produce sourdough characterized by specific sensory and functional fingerprints.

A review of free fatty acid determination methods for palm cooking oil

Free fatty acids (FFA) is one of the main content of palm cooking oil. High percentage of FFA in palm oil can affect human health and the quality of palm oil. Based on quality standards specification set by Malaysia Palm Oil Board (MPOB), the allowable content of free fatty acid (FFA) for crude palm oil (CPO) must not exceed 5%, meanwhile less than 0.1% of FFA in Refined Bleached Deodorized Oil (RBDO). There are many researches have been carried out to determine the level of FFA. However, they are using different type of cooking oil. A few researches are also reported regarding the palm cooking oil but they are using different method such as titration, spectroscopic, gas chromatography, reflection method and other type of detectors. All methods to determine the amount of free fatty acids (FFA) in palm cooking oil are reviewed in this paper.

Evaluation of physicochemical, rheological, microstructural, and microbial characteristics of synbiotic ultrafiltrated white cheese treated with transglutaminase

The quality parameters of the optimized symbiotic ultrafiltrated white cheese (OC; containing demineralized whey powder and inulin) treated with microbial transglutaminase enzyme (MTE) was compared with two commercial probiotic (PC) and non-probiotic (NC) cheeses. The counts of probiotic (Lactobacillus acidophilus LA5) and total lactic acid bacteria, and pH were significantly decreased during the 60-day cold storage period. Fat and protein contents, stress levels at the rupture point, and Young's modulus were meaningfully increased until the 30th day of storage, and thereafter were decreased. The amount of free amino acids in OC, PC, and NC cheeses were 1,440.25, 829.02, and 616.43 μmol/L, respectively. The total amount of free fatty acids in OC, PC, and NC cheeses were 100.06, 96.79, and 90.77 mg/100 g, respectively. Results showed that OC cheese had a more compact and less porous protein structure and maintained its superior texture score until the end of the storage period. Practical applications This research article deals with improving the texture of a synbiotic ultrafiltrated Iranian white cheese using whey powder and inulin (as prebiotics) and enzymatic treatment by microbial transglutaminase. In this investigation, an optimized synbiotic cheese was produced and compared with two commercial (non-probiotic and probiotic) cheeses based on physicochemical, rheological, microstructural, and microbial characteristics of the product. We found that the optimized cheese had superior texture as compare to control cheeses. The count of probiotic bacteria in both probiotic and synbiotic cheeses throughout the storage period was more than standard (>107). Therefore, our findings may likely to be of great interest to the vision of scientists, dairy technologists, and cheese-making industries.

Effect of NaCl Partial Replacement by Chloride Salts on Physicochemical Characteristics, Volatile Compounds and Sensorial Properties of Dry-Cured Deer Cecina.

The present study aimed to evaluate the effect of NaCl replacement in the physicochemical quality and volatile and sensorial profile of dry-cured deer cecina. Two salt mixtures were used as NaCl substitute: mixture I (30% NaCl-70% KCl) and mixture II (30% NaCl-50% KCl-15% CaCl2-5% MgCl2). Regarding the physicochemical parameters, only ash content, pH and L* values were affected by NaCl replacement. However, lipid oxidation was affected by NaCl replacement. The greatest thiobarbituric acid reactive substances (TBARS) values were observed in the control batch (3.28 mg MDA/kg). The partial replacement of NaCl by salt mixtures affected (p < 0.001) Ca, K, Mg, and Na content. The total amounts of free fatty acids and free amino acids were not affected (p > 0.05) by NaCl replacement. Concerning the volatile compounds, control samples presented the highest concentrations of furans (p < 0.01), while samples produced with mixture II had the lowest (p < 0.001) amounts of esters and acids. Our results indicated that all sensory attributes of the attribute map were affected (generalized procrustes analysis (GPA) explained 100% of the total variability among treatments). Considering the results obtained from the sensorial analysis, only mixture II reduced the overall acceptance and preference of consumers. Control attained significantly (p < 0.05) greater scores of acceptance and preference than mixture II despite the higher TBARS content.

Food-Grade Titanium Dioxide Particles Decreased the Bioaccessibility of Vitamin D3 in the Simulated Human Gastrointestinal Tract.

Food-grade titanium dioxide (E171) particles, as a "whiteness" additive, are often co-ingested with lipid-rich foods. Therefore, we explored the impact of E171 on lipid digestion and vitamin D3 (VD3) bioaccessibility encapsulated within oil-in-water emulsions in a simulated human gastrointestinal tract (GIT) model. VD3 bioaccessibility significantly decreased from 80 to 74% when raising E171 from 0 to 0.5 wt %. The extent of lipid digestion was reduced by E171 addition in a dose-dependent manner. VD3 bioaccessibility was positively correlated with the final amount of free fatty acids (FFAs) produced by lipid digestion (R2 = 0.95), suggesting that the reduction in VD3 bioaccessibility was due to the inhibition of lipid digestion by E171. Further experiments showed that E171 interacted with lipase and calcium ions, thereby interfering with lipid digestion. The findings of this study enhance our understanding toward the potential impact of E171 on the nutritional attributes of foods for human digestion health.

Improving heterogeneously catalyzed transesterification reaction for biodiesel production using ultrasound energy and petro-diesel as cosolvent

ABSTRACT Heterogeneously catalyzed transesterification process has by far been one of the most popular methods of biodiesel production particularly for the oil feedstock containing large amount of free fatty acid. However, the major constraint of the method is the poor yield of biodiesel and a long reaction time that increased its production cost. With an objective to increase the product yield in lesser time, the current work investigated the effect of addition of ultrasound and addition of cosolvent in the heterogeneously catalyzed transesterification reaction. Three commonly used solid catalyst – calcium oxide (CaO), calcined sodium silicate (CSS) and sulfated zirconia (SZ) were chosen for study. The results suggested that there was a remarkable increase in the yield of biodiesel in comparison to the conventional mechanically stirred process. CSS showed the best catalytic activity both in first use and in repeated uses. It gave a maximum yield of 97% biodiesel in 120 minutes with 9:1 molar ratio of alcohol to oil 3 wt.% of catalyst and 10 wt.% of catalyst. For CaO and CSS a noticeable difference in biodiesel yield was observed upon addition of cosolvent. CaO gave 76% yield with cosolvent and 65% without it. SZ gave 73% biodiesel with cosolvent and 61% without. Since petrodiesel was used as the cosolvent it was not required to remove the cosolvent from biodiesel during the purification process.