Physicochemical properties of three biofuels suitable for combustion in automotive diesel engines were compared. Two samples represented commercial hydrotreated vegetable oil (HVO) with different low temperature properties, one sample represented traditional biodiesel, fatty acid methyl esters (FAME). One HVO sample and FAME were blended into mineral diesel fuel to form two series of mixed fuels. One series represented mixed fuels containing 7, 20, 30, and 50 vol.% of HVO, the other series represented mixed fuels containing the same amount of FAME. All pure fuels as well as all prepared blends were then analyzed and evaluated. The composition and properties of HVO samples are discussed in detail as well as the influence of biocomponents on properties of mixed fuels.