Alpha-lipoic Acid Supplementation Research Articles

DIETARY SUPPLEMENTATION WITH VITAMIN E AND ALPHA-LIPOIC ACID REDUCES MYOCARDIAL ISCHEMIA-REPERFUSION INJURY IN VIVO

75 These experiments examined the effects of dietary supplementation of vitamin E (VE) and alpha-lipoic acid (LA) on myocardial lipid peroxidation following in vivo ischemia and reperfusion (I-R). Female Sprague-Dawley rats (4 months old) were assigned to one of four dietary treatments: 1) control diet (75 IU VE/kg diet), CON; 2) vitamin E deficient diet, DEF; 3) vitamin E and alpha-lipoic acid supplementation (10,000 IU VE/kg diet and 1.6 g LA/kg diet, diet duration=7-9 weeks), SUPP7-9; or 4) vitamin E and alpha-lipoic acid supplementation (10,000 IU VE/kg diet and 1.6 g LA/kg diet, diet duration=10-12 weeks), SUPP10-12. Following the supplementation period, significant differences existed (p<0.05) in myocardial vitamin E levels between all experimental groups (mean values: DEF=12.6, CON=45.3, SUPP7-9=83.7, SUPP10-12=245.6 μg/g wet weight). A significant correlation(r=0.91) existed between diet duration and myocardial vitamin E concentration in the SUPP animals. Following the feeding period, animals in each experimental group were subjected to an in vivo I-R protocol (25 minutes ischemia, 10 minutes reperfusion). Following I-R, SUPP10-12 animals had significantly lower (p<0.05) levels of myocardial lipid peroxidation compared to other experimental groups. These data suggest that 10 weeks of dietary supplementation of vitamin E and alpha-lipoic acid reduce myocardial lipid peroxidation resulting from an I-R insult.