Organic anion transport polypeptide 2 (OATP2), also known as OATP-C, OATP1B1, and LST-1, is responsible for the transportation of organic anions into hepatocytes. It has been reported that OATP2 transports a broad range of endogenous and xenobiotic compounds such as bile acids, bilirubin, sulfate and glucuronide conjugates, thyroid hormones, peptides, and drugs like 3-hydroxyl-3 methylglutaryl-coenzymeA–reductase inhibitors (pravastatin, rosuvastatin, pitavastatinand, and methorexate).1–3 The gene for OATP2 is located at chromosome 12p12.14.1, and a number of single-nucleotide polymorphisms (SNPs) have been identified in both the encoding and regulation regions of the OATP2 gene in different populations of the world.3 Among the Asian populations, two OATP2 variants have been shown to be highly prevalent in the Chinese in mainland China, that is, c.388A>G (73.4%) and c.521T>C (14.0%).4 Additionally, two other silent mutations, c.571T>C and c.597C>T of the OATP2 gene, were also reported to be common in Asian populations, occurring at 26% and 50%, respectively, in the Chinese population,5 and 64.2% and 42.9% in a Japanese population.4–7 The importance of OATP2 genotyping may lie in the fact that variations in the OATP2 gene may have an impact on drug metabolism and perhaps on the pathogenesis of neonatal jaundice. One study has shown that the OATP2 c.388A>G variant may be a possible risk factor for severe neonatal hyperbilirubinemia.8 Malaysia is a multiethnic country comprising of major ethnic Malays, Chinese, and Indians. This study forms our initial effort to determine the existence of the OATP2 gene variation in the Malaysian population as a prequel to study its role in cases of unconjugated hyperbilirubinemia, a common condition in Malaysian newborns. We report here our study on the determination of allelic variations in Malaysian neonates using an HRM analysis and TaqMan Minor Groove Binder (MGB) assays. Until now, these OATP2 alleles have been genotyped with either a polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) assay (c.388A>G) or with an allele-specific PCR assay (c.521T>C).3,9 Recent study reported the use of real-time PCR with the fluorescence resonance energy transfer (FRET) assay for genotyping the c.388A>G and c.521T>C variant.10 To perform large epidemiological studies or for routine clinical use, a rapid genotype method is preferred. Therefore, the present study describes the application of HRM assays and TaqMan MGB assays. By using these methods, we were able to genotype these OATP2 polymorphisms in considerably less hands-on time and with a reduced contamination risk.