Mixture Of Alkaloids Research Articles

Ultraselective, Ultrahigh Resolution 1D TOCSY

AbstractSolution state 1H NMR spectroscopy provides valuable insights into molecular structure and conformation. However, when the spectrum exhibits severe signal overlap, it hampers the extraction of key structural information. Here, an ultraselective, ultrahigh resolution TOCSY method is introduced that greatly reduces spectral complexity, allowing the extraction of previously inaccessible spectral information. It combines the recently developed GEMSTONE excitation with homonuclear decoupling to provide highly simplified through‐bond correlation 1D 1H NMR spectra, showing all signals within the selected spin system as singlets. The new method can greatly facilitate the analysis of mixtures, as shown here for a mixture of Cinchona alkaloids (popular catalysts in asymmetric synthesis) and a mixture of glucocorticoids (used for treating conditions such as asthma).

High deoxynivalenol and ergot alkaloid levels in wheat grain: effects on growth performance, carcass traits, rumen fermentation, and blood parameters of feedlot cattle

This study was designed to assess the impacts of a mixture of deoxynivalenol (DON) and ergot alkaloids (EAs) on growth performance, rumen function, blood parameters, and carcass traits of feedlot cattle. Forty steers (450 ± 6.0 kg) were stratified by weight and randomly allocated to 1 of 4 treatments; control-low (CON-L), control-high (CON-H) which contained low or high wheat screenings that lacked mycotoxins at the same level as the mycotoxin-low (MYC-L; 5.0 mg/kg DON, 2.1 mg/kg EA), and mycotoxin-high (MYC-H: 10 mg/kg DON, 4.2 mg/kg EA) diets that included wheat screening with mycotoxins. Steers were housed in individual pens for a 112-day finishing trial. Intake was 24.8% lower (P < 0.001) for MYC steers compared to CON steers. As a result, average daily gains of MYC steers were 42.1% lower (P < 0.001) than CON steers. Gain to feed ratio was also lower (P < 0.001) for MYC steers compared to CON steers. Platelets, alanine aminotransferase, globulins, and blood urea nitrogen were lower (P ≤ 0.008), and lymphocytes, glutathione peroxidase activity (GPx), and interleukin-10 (IL-10) were elevated (P ≤ 0.002) in MYC steers compared to CON steers. Hot carcass weights and backfat thickness were reduced (P < 0.001) in MYC steers, resulting in leaner (P < 0.001) carcasses and higher (P < 0.007) meat yield compared to CON steers. Results suggest that a mixture of DON and EAs negatively impacted health, performance, and carcass traits of feedlot steers, with the majority of this response likely attributable to EAs. However, more research is needed to distinguish the relative contribution of each mycotoxin to the specific responses observed.

Pharmaco-therapeutic potentials of Mucuna pruriens (L.) in male infertility and associated sexual dysfunctions

Abstract Mucuna pruriens (L.) (MP), also known as Kapikacchu in Sanskrit, is a well-known medicinal plant, the seed of which is commonly used in traditional systems of medicine worldwide, mainly for treating male infertility and associated conditions. Infertility is a multifactorial disease process with several potential contributing causes, among which male factors contribute to almost 50% of cases of infertility, the causative factors of which can be counted from faulty lifestyle to stress factors. In Ayurvedic system of medicine, various formulations of MP are explained in Vajikarana (Aphrodisiac) context. In this review, Pubmed, Google Scholar, Scopemed, and other online databases, including fields of pharmacology, pharmacognosy, and health, and published chapters of both classical and contemporary books, were rationally scrutinized to find out the scope, research possibilities, and potential role in improving male infertility and associated sexual dysfunctions. After thorough scrutiny, it has been observed that MP has an action on multiple aspects of infertility like aging, depression, cardiovascular diseases, diabetes mellitus, and Parkinson’s disease due to the presence of a complex mixture of alkaloids (like L-DOPA and its metabolites) and its nutritional component, along with its antioxidant and adaptogenic properties, which raises hope for the researchers to explore the effect of the drug in more accurate and aggressive way to fulfill the demand of the global issue.

Integrating transcriptomics and network pharmacology to reveal the mechanisms of total Rhizoma Coptidis alkaloids against nonalcoholic steatohepatitis

Ethnopharmacological relevanceNon-alcoholic steatohepatitis (NASH) has emerged as a major cause of cirrhosis and hepatocellular carcinoma, posing a significant threat to public health. Rhizoma Coptidis, a traditional Chinese medicinal herb has been shown to have significant curative effects on liver diseases. Total Rhizoma Coptidis Alkaloids (TRCA) is a primarily alkaloid mixture extracted from Rhizoma Coptidis, and its constituents are widely accepted to have hepatoprotective effects. Aim of the studyThis work aimed to investigate the efficacy and potential mechanisms of TRCA in ameliorating NASH through both in vitro experiments and in vivo mouse models. Materials and methodsThe study employed a mice model induced by a high-fat diet (HFD) to evaluate the effectiveness and pharmacological mechanisms of TRCA in alleviating NASH. Transcriptomic sequencing and network pharmacology were used to explore the possible targets and mechanisms of TRCA to ameliorate NASH. Further validation was performed in free fatty acid (FFA)-induced human hepatocytes (LO2) and human hepatocellular carcinoma cells (HepG2). ResultsTRCA effectively ameliorated the main features of NASH such as lipid accumulation, hepatitis and hepatic fibrosis in the liver tissue of mice induced by HFD, as well as improved glucose tolerance and insulin resistance in mice. Combined with transcriptomic and network pharmacological analyses, 68 core targets associated with the improvement of NASH by TRCA were obtained. According to the KEGG results, the core targets were significantly enriched in the PI3K-AKT signaling pathway whereas TRCA ameliorated the aberrant down-regulation of the PI3K-AKT signaling pathway induced by HFD. Furthermore, the five highest-ranked genes were obtained by PPI network analysis. Moreover, our findings suggest that TRCA may impede the progression of HFD-induced NASH by regulating the expression of PPARG, MMP9, ALB, CCL2, and EGFR. ConclusionsTRCA can ameliorate HFD-induced liver injury by modulating aberrant downregulation of the PI3K-AKT signaling pathway. Key proteins such as PPARG, MMP9, ALB, CCL2, and EGFR may be critical targets for TRCA to ameliorate NASH. This finding supports using Rhizoma Coptidis, a well-known herbal medicine, as a potential therapeutic agent for NASH.

Natural Compounds Purified from the Leaves of Aristotelia chilensis: Makomakinol, a New Alkaloid and the Effect of Aristoteline and Hobartine on NaV Channels.

Aristotelia chilensis or "maqui" is a tree native to Chile used in the folk medicine of the Mapuche people as an anti-inflammatory agent for the treatment of digestive ailments, fever, and skin lesions. Maqui fruits are black berries which are considered a "superfruit" with notable potential health benefits, promoted to be an antioxidant, cardioprotective, and anti-inflammatory. Maqui leaves contain non-iridoid monoterpene indole alkaloids which have previously been shown to act on nicotinic acetylcholine receptors, potassium channels, and calcium channels. Here, we isolated a new alkaloid from maqui leaves, now called makomakinol, together with the known alkaloids aristoteline, hobartine, and 3-formylindole. Moreover, the polyphenols quercetine, ethyl caffeate, and the terpenes, dihydro-β-ionone and terpin hydrate, were also obtained. In light of the reported analgesic and anti-nociceptive properties of A. chilensis, in particular a crude mixture of alkaloids containing aristoteline and hobartinol (PMID 21585384), we therefore evaluated the activity of aristoteline and hobartine on NaV1.8, a key NaV isoform involved in nociception, using automated whole-cell patch-clamp electrophysiology. Aristoteline and hobartine both inhibited Nav1.8 with an IC50 of 68 ± 3 µM and 54 ± 1 µM, respectively. Hobartine caused a hyperpolarizing shift of the voltage-dependence of the activation, whereas aristoteline did not change the voltage-dependence of the activation or inactivation. The inhibitory activity of these alkaloids on NaV channels may contribute to the reported analgesic properties of Aristotelia chilensis used by the Mapuche people.

Corydalis saxicola Bunting total alkaloids improve NAFLD by suppressing de novo lipogenesis through the AMPK-SREBP1 axis

Ethnopharmacological relevanceAlong with the gradually increasing incidence, nonalcoholic fatty liver disease (NAFLD) has already been influencing the health of more and more people in the world. Corydalis saxicola Bunting (CSB), a valuable folk medicine, is the dried whole grass of a perennial herb, Yanhuanglian (Papaveraceae), which has significant effects on various hepatitis, liver fibrosis, cirrhosis and other liver diseases. Corydalis saxicola Bunting total alkaloids (CSBTA), a mixture of alkaloids extracted from CSB, exhibit widely-accepted hepatoprotective effects. Aim of the studyThis study aimed to explore the therapeutic potential of CSBTA on NAFLD and the underlying mechanism. Materials and methodsA mice model was established by high fat and high cholesterol diet (HFHCD) to study the benefits of CSBTA on the progression of NAFLD. The efficacy of CSBTA on NAFLD was revealed systematically via RNA-sequencing analysis. Further efficacy and molecular mechanism study were explored in mouse primary hepatocytes and HepG2 cells stimulated with high energy with or without pharmacological inhibition or gene silencing. ResultsCSBTA effectively improved the major hallmarks of NAFLD including liver lipid accumulation, liver injury, inflammation and fibrosis in HFHCD-fed mice. RNA sequencing and targeted qPCR analysis jointly evidenced CSBTA significantly suppressed the expression of Srebf1, Acc1 and Fasn which are the genes responsible for fatty acid biosynthesis. Moreover, stable isotope tracer test denoted CSBTA reduced lipid accumulation via interrupting fatty acid biosynthesis in hepatocytes or the liver. Mechanistically, CSBTA could impede SREBP1 maturation via AMPK activation, thereby reducing DNL-derived lipid accumulation in hepatocytes. ConclusionsCSBTA protected against hepatic steatosis and other hallmarks of NAFLD induced by HFHCD via suppressing DNL, through modulating the AMPK-SREBP1 axis. CSBTA may therefore have a therapeutic potential for NAFLD treatment.

New Imidazolium Alkaloids with Broad Spectrum of Action from the Marine Bacterium Shewanella aquimarina.

The continuous outbreak of drug-resistant bacterial and viral infections imposes the need to search for new drug candidates. Natural products from marine bacteria still inspire the design of pharmaceuticals. Indeed, marine bacteria have unique metabolic flexibility to inhabit each ecological niche, thus expanding their biosynthetic ability to assemble unprecedented molecules. The One-Strain-Many-Compounds approach and tandem mass spectrometry allowed the discovery of a Shewanella aquimarina strain as a source of novel imidazolium alkaloids via molecular networking. The alkaloid mixture was shown to exert bioactivities such as: (a) antibacterial activity against antibiotic-resistant Staphylococcus aureus clinical isolates at 100 µg/mL, (b) synergistic effects with tigecycline and linezolid, (c) restoration of MRSA sensitivity to fosfomycin, and (d) interference with the biofilm formation of S. aureus 6538 and MRSA. Moreover, the mixture showed antiviral activity against viruses with and without envelopes. Indeed, it inhibited the entry of coronavirus HcoV-229E and herpes simplex viruses into human cells and inactivated poliovirus PV-1 in post-infection assay at 200 µg/mL. Finally, at the same concentration, the fraction showed anthelminthic activity against Caenorhabditis elegans, causing 99% mortality after 48 h. The broad-spectrum activities of these compounds are partially due to their biosurfactant behavior and make them promising candidates for breaking down drug-resistant infectious diseases.

Central nervous system activity of a Tabernaemontana arborea alkaloid extract involves serotonergic and opioidergic neurotransmission in murine models

Tabernaemontana arborea (Apocynaceae) is a Mexican tree species known to contain ibogan type alkaloids. This study aimed at determining central nervous system-related activities of an alkaloid extract obtained from the root bark of T. arborea. A gas chromatography–mass spectrometry (GC–MS) analysis was performed to describe the alkaloid profile of the extract. A wide dosing range (0.1 to 56.2 mg/kg) of this extract was evaluated in different murine models. Electrical brain activity was examined by electroencephalography (EEG). The extract's effects on motor coordination, ambulatory activity, and memory were analyzed based on the rotarod, open field (OFT), and object recognition tests (ORT), respectively. Antidepressant and antinociceptive activities were determined using the forced swimming test (FST) and the formalin assay, respectively. In order to elucidate the underlying mechanisms of action, the 5-HT1A receptor antagonist WAY100635 (1 mg/kg) or the opioid receptor antagonist naloxone (1 mg/kg) was included in the latter experiments.GC–MS analysis (μg/mg extract) confirmed the presence of the monoterpenoid indole alkaloids (MIAs) voacangine (207.00), ibogaine (106.33), vobasine (72.81), coronaridine (30.72), and ibogamine (24.2) as principal constituents of the extract, which exhibited dose- and receptor-dependent antidepressant (0.1 to 1 mg/kg; 5-HT1A) and antinociceptive (30 and 56.2 mg/kg; opioid) effects, without altering motor coordination, ambulatory activity, and memory. EEG indicated CNS depressant activity at high doses (30 and 56.2 mg/kg). The root bark of T. arborea contains a mixture of alkaloids that may hold therapeutic value in pain relief and the treatment of psychiatric diseases without causing neurotoxic activity at effective doses.

In Vitro and In Vivo Therapeutic Potential of 6,6'-Dihydroxythiobinupharidine (DTBN) from Nuphar lutea on Cells and K18-hACE2 Mice Infected with SARS-CoV-2.

We have previously published research on the anti-viral properties of an alkaloid mixture extracted from Nuphar lutea, the major components of the partially purified mixture found by NMR analysis. These are mostly dimeric sesquiterpene thioalkaloids called thiobinupharidines and thiobinuphlutidines against the negative strand RNA measles virus (MV). We have previously reported that this extract inhibits the MV as well as its ability to downregulate several MV proteins in persistently MV-infected cells, especially the P (phospho)-protein. Based on our observation that the Nuphar extract is effective in vitro against the MV, and the immediate need that the coronavirus disease 2019 (COVID-19) pandemic created, we tested here the ability of 6,6'-dihydroxythiobinupharidine DTBN, an active small molecule, isolated from the Nuphar lutea extract, on COVID-19. As shown here, DTBN effectively inhibits SARS-CoV-2 production in Vero E6 cells at non-cytotoxic concentrations. The short-term daily administration of DTBN to infected mice delayed the occurrence of severe clinical outcomes, lowered virus levels in the lungs and improved survival with minimal changes in lung histology. The viral load on lungs was significantly reduced in the treated mice. DTBN is a pleiotropic small molecule with multiple targets. Its anti-inflammatory properties affect a variety of pathogens including SARS-CoV-2 as shown here. Its activity appears to target both pathogen specific (as suggested by docking analysis) as well as cellular proteins, such as NF-κB, PKCs, cathepsins and topoisomerase 2, that we have previously identified in our work. Thus, this combined double action of virus inhibition and anti-inflammatory activity may enhance the overall effectivity of DTBN. The promising results from this proof-of-concept in vitro and in vivo preclinical study should encourage future studies to optimize the use of DTBN and/or its molecular derivatives against this and other related viruses.

Influence of Elicitation on Glaucium flavum Cultures in in Vitro Conditions

Glaucium flavum Crantz is a medicinal plant species which contains high variety of isoquinoline alkaloids, the main of which is the alkaloid glaucine, possessing antitussive activity. In vitro cultivation was investigated as an alternative and sustainable approach for plant cultivation of this species which is characterized by decreasing distribution. The biotechnological production of secondary metabolites could be improved by the addition of elicitors such as jasmonic acid, methyl jasmonate and yeast extract to the artificial medium. The seed germination of G. flavum was over 60% in all examined media supplemented with the abovementioned elicitors. They did not stimulate higher alkaloid production but the plants of some of the investigated in vitro cultures had similar percentage of the crude alkaloid mixture, about 3%, to the native plant population from which they had derived. The quantity of the alkaloids varied but their content in the studied samples remained similar.

Directed Biosynthesis of Mitragynine Stereoisomers.

Mitragyna speciosa ("kratom") is used as a natural remedy for pain and management of opioid dependence. The pharmacological properties of kratom have been linked to a complex mixture of monoterpene indole alkaloids, most notably mitragynine. Here, we report the central biosynthetic steps responsible for the scaffold formation of mitragynine and related corynanthe-type alkaloids. We illuminate the mechanistic basis by which the key stereogenic center of this scaffold is formed. These discoveries were leveraged for the enzymatic production of mitragynine, the C-20 epimer speciogynine, and fluorinated analogues.

The in vitro genotoxicity potency of mixtures of pyrrolizidine alkaloids can be explained by dose addition of the individual mixture components.

Plant-based 1,2-unsaturated Pyrrolizidine Alkaloids (PAs) are responsible for liver genotoxicity/carcinogenicity following metabolic activation, making them a relevant concern for safety assessment. Due to 21st century toxicology approaches, risk of PAs can be better discerned though an understanding of differing toxic potencies, but it is often mixtures of PAs that are found as contaminants in foods, for example, herbal teas and honey, food supplements and herbal medicines. Our study investigated whether genotoxicity potency of PAs dosed individually or in mixtures differed when measured using micronuclei formation in vitro in HepaRG human liver cells, which we and others have shown to be suitable for observing genotoxic potency differences across different PA structural classes. When equipotent concentrations of up to six different PAs representing a wide range of potencies in vitro were tested as mixtures, the observed genotoxic potency aligned favorably with results for single PAs. Similarly, when the BMD confidence intervals of these equipotent mixtures were compared with the confidence intervals of the individual PAs, only minimal variation was observed. These data support a conclusion that for this class of plant impurities, all acting via the same DNA-reactive mode of action, genotoxic potency can be regarded as additive when assessing the risk of mixtures of PAs.

Effective Solid Phase Extraction of Toxic Pyrrolizidine Alkaloids from Honey with Reusable Organosilyl-Sulfonated Halloysite Nanotubes

Pyrrolizidine alkaloids are plant secondary metabolites that have recently attracted attention as toxic contaminants in various foods and feeds as they are often harvested by accident. Furthermore, they prove themselves as hard to analyze due to their wide structural range and low concentration levels. However, even low concentrations show toxic behavior in the form of chronic liver diseases and possible carcinogenicity. Since sample preparation for this compound group is in need of more green and sustainable alternatives, modified halloysite nanotubes present an interesting approach. Based on the successful use of sulfonated halloysite nanotubes as inexpensive, easy-to-produce cation exchangers for solid phase extraction in our last work, this study deals with the further modification of the raw nanotubes and their performance in the solid phase extraction of pyrrolizidine alkaloids. Conducting already published syntheses of two organosilyl-sulfonated halloysite nanotubes, namely HNT-PhSO3H and HNT-MPTMS-SO3H, both materials were used as novel materials in solid phase extraction. After the optimization of the extraction protocol, extractions of aqueous pyrrolizidine alkaloid mixtures showed promising results with recoveries ranging from 78.3% to 101.3%. Therefore, spiked honey samples were extracted with an adjusted protocol. The mercaptopropyl-sulfonated halloysite nanotubes revealed satisfying loading efficiencies and recoveries. Validation was then performed, which displayed acceptable performance for the presented method. In addition, reusability studies using HNT-MPTMS-SO3H for solid phase extraction of an aqueous pyrrolizidine alkaloid mixture demonstrated excellent results over six cycles with no trend of recovery reduction or material depletion. Therefore, organosilyl-sulfonated halloysite nanotubes display a green, efficient and low-cost alternative to polymeric support in solid phase extraction of toxic pyrrolizidine alkaloids from complex honey matrix.

Anastomosing laticifer in the primary and secondary structures of Calotropis procera (Aiton) W.T.Aiton (Apocynaceae) stems.

An in-depth understanding of the development and distribution of laticifer (latex secretory structure) will be important for the production of both rubber and medicines and will support studies on plant adaptations to their environments. We characterize here and describe the ontogenesis of the laticifer sytem in Calotropis procera (Apocynaceae), an invasive subshrub species in arid landscapes. Anatomical and histochemical evaluations of the primary and secondary structures of the stem were carried out on a monthly basis during a full year, with ultrastructural evaluations of laticifer on the stem apex during the rainy season. In the primary structure, laticifer differentiate early from procambium and ground meristem cells of the cortex and medulla and become concentrated adjacent to the external and internal phloem of the bicollateral bundles. In the secondary structure, laticifer differentiates from fusiform derivative cells of the phloem close to the sieve-tube elements. The laticifer is of the articulated, anastomosing, branched type, and it originates from precursor cells that loose the transversal and longitudinal walls by dissolution. Latex is a mixture of terpenes, alkaloids, flavonoids, mucilage, and proteins. The apical meristem and vascular cambium where the laticifer system begins its development are active throughout the year, including during the dry season. The vascular cambium produces phloem with laticifer precursor cells during the rainy season, with high temperatures and long days. The ability of C. procera to grow under water deficit conditions and produce laticifer throughout the year contribute to its wide distribution in arid environments.

Alkaloids from Pandanus amaryllifolius and Pandanus tectorius from Vietnam and Their Anti-inflammatory Properties

Phytochemical investigation of the alkaloid composition in Pandanus amaryllifolius Roxb. and Pandanus tectorius Parkinson was carried out. Nine pandane alkaloids were isolated and determined. The alkaloids pandamarilactonine B, N-acetylnorpandamarilactonine A and pandamarilactonine G were found for the first time in P. tectorius. The total alkaloid mixtures and alkaloid fractions of the species were evaluated for their anti-inflammatory activity using in vitro experimental models.

FORMULASI DAN UJI EKTIVITAS ANTIBAKTERI SEDIAAN LOTION EKSTRAK ETANOL DAUN SAWAO TERHADAP BAKTERI Stapylococcuc aureus dan Escherchia coli

Manila sapodilla leaves contain phytochemical mixtures of alkaloids, flavonoids, saponins, tannins, terpenoids and glycosides which are known to have antibacterial movement of Staphylococcus aureus and Escherichia coli. The reason for this study was whether the planning of sapodilla leaf extricate salve can be utilized as an enemy of bacterial technique. This exploration was led utilizing a trial which plans to decide all side effects or impacts that emerge, because of specific medicines. Sawo Leaf Ethanol Extract was completed by submerging maceration utilizing 96% ethanol. Antibacterial action testing was done on Sawo leaf extricate with different centralizations of 5%, 10% and 20% and Chaladine cream as a positive control was completed utilizing the discdiffusion strategy. The consequences of the restraint zone movement of Staphylococcus aureus antibacterial lotio arrangements Chaladine sapodilla leaf remove 18 mm, Extract focus 5% 14.5 mm, Extract fixation 10% 20.3 mm, Extract focus 20% 15.46 mm, The aftereffects of the inhibitory zone action in the cream readiness antibacterial concentrate of sapodilla lotio leaf Escherichia coli lotio Chaladine 14 mm, Extract focus 5% 14.16 mm, Extract fixation 10% 20.3 mm, Extract fixation 20% 16.06 mm &gt; 0.05, this demonstrates that the information are typically dispersed, homogeneity trial of 2 gatherings of microscopic organisms and shows a meaning of p &gt; 0.05, showing that the information utilized came from homogeneous information. This demonstrates that Lotio ethanol concentrate of sapodilla leaves has antibacterial movement.

Standardization of protocol for shoot regeneration in Bacopa monnieri (L.) Pennell using leaf explants

Bacopa monnieri (L.) Pennell commonly called ‘Brahmi’ is used as a medicinal herb since ages. It is known for its revitalizing and nootropic actions in Ayurvedic medicine as it strengthens memory as well as intellect. This activity of the plant has been accredited to a mixture of triterpenoid saponins, sterols and alkaloids. Of these saponins, Bacoside-A and Bacoside-B have been found to be the most important. Present work aimed to establish direct and indirect shoot regeneration protocol in Bacopa monnieri (L.) Pennell using leaf explants. It was found that MS medium supplemented with 1.0 mg/l 2, 4-D and 0.5 mg/l BAP was best for callus formation (53.33%). Leaf explants showed both direct and indirect regeneration and MS medium supplemented with 6.0 μ mol/ was found to be best for shoot regeneration.

Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures.

Diffusion-ordered spectroscopy (DOSY) is a powerful tool for investigating mixtures and identifying peaks of chemical components. However, similar diffusion coefficients of the components, particularly for complex mixtures that contain crowded resonances, limit resolution and restrict application of the DOSY technique. In this paper, matrix-assisted DOSY were used to explore whether the diffusion resolution of a complex model involving indole alkaloid mixtures can be realized. Furthermore, we investigated the influence of different factors on the separation effect. The results showed that the changes in diffusion coefficient differences were achieved more obviously when using sodium dodecyl sulfate (SDS) micelles as the matrix. In addition, we also found that increasing the concentration of SDS can improve the resolution of the DOSY spectrum. Finally, after investigating the influence factors and NMR conditions, we demonstrated the applications of the SDS-assisted DOSY on analyzing the total alkaloid extract of Alstonia Mairei, and the virtual separation of mixtures was achieved.

Antifungal Activity of New Diterpenoid Alkaloids Isolated by Different Chromatographic Methods from Delphinium peregrinum L. var. eriocarpum Boiss.

This paper aimed to investigate the potential antifungal influences of new alkaloids from Delphinium peregrinum L. var. eriocarpum Boiss. New Diterpenoid alkaloids Delcarpum (1), Hydrodavisine (4) and known alkaloids Peregrine (2), Delphitisine (3) were isolated by different chromatographic methods from the aerial parts of D. Peregrinum eriocarpum Boiss, which grows in Syria. The structures of alkaloids were proposed based on 1D NMR spectroscopy 1H-NMR, 13C-NMR, DEPT-135, DEPT-90, 2D NMR spectroscopy DQF-COSY, HMQC, EI-Ms mass spectrum, and IR spectroscopic measurements. The antifungal activity of the isolated alkaloids was evaluated against different dermatophyte fungal isolates compared with fluconazole. In the case of Peregrine (2) the minimum inhibitory concentrations(MICs) recorded 128–256, 32–64, and 32 for Epidermophyton floccosum, Microsporum canis, and Trichophyton rubrum, respectively, compared to 32–64, 16, and 32 μg/mL in the case of fluconazole, respectively. The MICs recorded on application of the four alkaloids mixture were 64, 32, and 16 in the case of E. floccosum, M. canis, and T. rubrum, respectively, which were significantly lower than that measured for each of the individual alkaloid and were compatible for fluconazole. In conclusion, MICs of the tested alkaloids showed a variable potential effect on the investigated fungal isolates. Peregrine (2) was the most effective alkaloid, however, the application of the mixture of alkaloids induced significant synergistic activity that was more pronounced than the application of individual ones.

In vitro propagation and biosynthesis of Sceletium-type alkaloids in Narcissus pallidulus andNarcissus cv. Hawera

In vitro cultures from Narcissus pallidulus and Narcissus cv. Hawera were initiated on MS medium supplemented with different concentrations of BAP and NAA. High multiplication rates were obtained on MS medium supplemented with 6 mg/L BAP and 0.1 mg/L NAA. Callus was induced on MS medium supplemented with 6 mg/L BAP and 2 mg/L NAA. Cultivation of the in vitro cultures on MS medium supplemented with 1 mg/L BAP and 0.1 mg/L NAA or with 1 g/L charcoal resulted in bulb growth. Well-developed bulbs with roots were successfully acclimatized. GC–MS analyses of the alkaloid patterns revealed that Narcissus cv. Hawera biosynthesizes Sceletium-type and Amaryllidaceae alkaloids, while Narcissus pallidulus accumulates only Sceletium-type compounds. The dominant compound in their alkaloid mixtures is mesembrenone which possesses potent antidepressant and antianxiety activities. The easy multiplication of these plants provides excellent possibilities for further studies on mesembrenone biosynthesis and development of technologies for its production, both in vitro and in vivo.