Screening was done from soil samples collected from black cotton soil, groundnut field, milk processing unit, Kotappakonda hill area in Guntur Dist,and Tirumala in Chittur District, A.P. Indian soils are best for alkaline protease producing bacteria that resulted in isolation of 21 alkaline protease producing alkaliphilic bacterial strains. MS6 showed the highest production (110U/ml) after 48h. This isolate was identified as Bacillus licheniformis by Microbial Type Culture Collection center (MTCC) and gene bank, Institute of Microbial Technology (IMTECH), Chandigarh, India. This strain was used to examine the changes in alkaline protease production following UV irradiation. Induction of mutation in Bacillus licheniformis strain was carried out by 0, 3, 6, 9, 12, 15, 18 and 20 min with 30-W germicidal lamp that has radiation at 2540 - 2550A 0 at a distance of 15cm in dark and irradiated. A total of 17 mutants were selected. They were designated as Bl1 to Bl9 and Bl10 to Bl17. Among these only three strains viz., Bl2, Bl11, and Bl16 did exhibit high efficiency in production on the basis of relative growth production (C/G). Of the seventeen mutants of Bacillus licheniformis, ten were chosen to assay their productivity. Mutants no Bl8, Bl3, Bl16 were the most effective in enzyme production under submerged conditions being 180, 140,128U/ml respectively. Results revealed that alkaline protease activity assay under submerged culture conditions was more accurate than the relative growth production (C/G) method because there is no correlation between zone diameter and the ability to produce the enzyme in submerged cultures. High level of productivity increased with Bl8 mutant of B.licheniformis, indicating that the enzyme is to be thermo-alkaliphilic protease.