A photoacoustic (PA) graphic equalization (PAGE) algorithm was developed to characterize the relative size of optical absorbing aggregates. This technique divides the PA signal into frequency bands related to different-sized optical absorbers. Simulations of a material containing optical absorbing microparticles of varying size were used to assess PAGE performance. Experiments were performed on phantom materials containing microspheres of varying size and concentration. Additional experiments were performed using tubes with fresh clotting blood. PA data was obtained using a Vevo LAZR-X system (FUJIFILM VisualSonics Inc). PAGE imaging of phantoms with varying-sized optical absorbers found a 1.5-fold difference in mean image intensity (p < 0.001). Conversely, PA images from these same materials exhibited no intensity changes (p = 0.68). PAGE imaging results from clotting blood exhibited differences for clot sizes in the range 0.30–0.64 mm (p < 0.001). In summary, PAGE imaging can distinguish optical absorbing aggregates of varying size.
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