Alcohol Solution Research Articles

The investigation on targeted micro-surface treatment methods for waste tires recycling in cementitious construction materials

Defects in the interfacial transition zone (ITZ) between recycled tire rubber and cement matrix obstacle to the large-scale application of sustainable rubberized cementitious materials. This paper proposed and evaluated different targeted treatment methods for micro-surface properties enhancement of rubber aggregate by the Polyvinyl Alcohol (PVA), Polyethylene Glycol-200 (PEG), and Hydroxypropyl methylcellulose (HY) polymer solutions through copolymerization and drafting processes. The optimum modification condition was determined through the FT-IR analysis, contact angle, and surface free energy of treated rubber aggregates and the micro-surface morphology and element distribution were analyzed by using ESEM and EDX, respectively. The flexural strength and pore size distribution of steel fiber-reinforced rubberized mortar with relatively high replacing volumes (20%, 40%, and 60%) were evaluated to validate the targeted micro-surface modification efficiency. The surface microstructure and hydrophilic properties of rubber aggregate were significantly improved and the mechanism for enhancing the interface adhesion between rubber and cement matrix was revealed.

Gas–Liquid Mass Transfer Intensification for Selective Alkyne Semi-Hydrogenation with an Advanced Elastic Catalytic Foam-Bed Reactor

The Elastic Catalytic Foam-bed Reactor (EcFR) technology was used to enhance a model catalytic hydrogenation reaction by improving gas–liquid mass transfer. This advanced technology is based on a column packed with a commercial elastomeric polyurethane open-cell foam, which also acts as a catalyst support. A simple and efficient crankshaft-inspired system applied in situ compression/relaxation movements to the foam bed. For the first time, the catalytic support parameters (i.e., porosity, tortuosity, characteristic length, etc.) underwent cyclic and controlled changes over time. These dynamic cycles have made it possible to intensify the transfer of gas to liquid at a constant energy level. The application chosen was the selective hydrogenation of phenylacetylene to styrene in an alcoholic solution using a palladium-based catalyst under hydrogen bubble conditions. The conversion observed with this EcFR at 1 Hz as cycle frequency was compared with that observed with a conventional Fixed Catalytic Foam-bed Reactor (FcFR).

The use of plant explants to obtain embryogenic callus clams as an early stage in the selection of Napier Grass cv Taiwan

Abstract This study aimed to obtain 50-100 mussels from a population of embryogenic callus derived from cultivars of Taiwan elephant grass. The research was conducted in three phases: 1) sterilization, which was accomplished by immersing the material in 70% alcohol solution, 20% chlorox, and rinsing it with sterile distilled water so that it could be cultured in the treatment media; 2) callus induction with explants of young leaves in culture medium supplemented with growth regulator (PGR) 2,4-D at a concentration of 2 mg/l and enriched with amino acids and casein hydrolysate; 3) callus proliferation and regeneration utilizing culture-based. The results indicated that the success rate of explant sterilization ranged from 10 to 78%, and the ability of the tissue to form callus ranged from 60 to 80%, with the highest callus-forming ability obtained from culture explants with chemical and physical combination sterilization by burning, while at the proliferation stage, it was obtained on average 5 times per series. After two weeks, the average callus weight was 1.72 to 1.76 g per bottle, increasing to 1.92 to 2.07 g per bottle for a total of 150 bottles, with 78.1 to 85.3% of callus cells capable of regenerating into shoots via organogenesis or embryogenesis. It was determined that the initial selection procedure for fodder plants from explants to obtain callus has the potential to be further developed.

Polymerase Chain Reaction Cleaner with Antibacterial Effect and Faster Elimination of Nucleic Acid Aerosol Pollution

With advancement of nucleic acid detection technology, most universities, biological testing companies, and hospitals have Polymerase Chain Reaction (PCR) laboratories. PCR detection technology is the core technology for nucleic acid detection. When nucleic acid detection is performed in a PCR laboratory, nucleic acid aerosol samples are often dispersed to the environment in the form of aerosols. At this time, there will be some nucleic acid contamination in the PCR laboratory, resulting in false positive samples. The purpose of this paper is to propose a new type of nucleic acid pollution scavenger called PCR Cleaner. Firstly, the best ratio of PCR Cleaner was obtained by a control experiment, and then the antibacterial test for the PCR Cleaner was carried by comparing the nucleic acid pollution removal efficiency of different ratios of PCR Cleaner and common nucleic acid pollution scavenger on the surface and in the air. Experiment results showed that, the removal efficiency of PCR Cleaner on the surface of nucleic acid was much higher than that of alcohol and aqueous solution. Its effect was good enough when compared to the two commonly used nucleic acid pollution scavengers (DNA/RNA-ExitusPlus and PCR clean). The antibacterial and bacteriostatic PCR Cleaner can significantly inhibit the growth of high concentration of E. coli, and can also completely inhibit the low concentration of E. coli.

Enhanced gelling property and freeze-thaw stability of potato, tapioca and corn starches modified by mild heating in aqueous ethanol solution.

Physically modified starches can be classified as natural ingredients on food labels and clean label products. Thus, the market demand for physically modified starch is increasing. Potato, tapioca and corn starches were physically modified by mild heat treatment in an alcoholic solution to enhance their gelling property and freeze-thaw stability. During mild heating of starch suspension (40% w/w) in 10% ethanol solution at the onset gelatinization temperature, granular swelling of starch occurred, followed by amylose leaching with medication of the surface structure of the starch granules. All treated starches exhibited increased gelatinization and pasting temperatures and decreased breakdown for pasting as a result of improved stability against shear and heat. The treated starches had higher hardness, cohesiveness and springiness of gel than the respective native starches, and these gel properties were more pronounced in potato starch than in tapioca and corn starches. The treated starches showed substantially reduced gel syneresis during freeze-thawing. Physical modification of starch by mild heat treatment in an alcoholic solution substantially improved its gelation ability and freeze-thaw stability. © 2024 Society of Chemical Industry.

Formulation, in vitro and in vivo evaluation of olanzapine nanoparticles dissolving microneedles for transdermal delivery

Olanzapine (OLZ) is classified as a typical antipsychotic drug utilized for the treatment of schizophrenia. Its oral bioavailability is 60% due to its low solubility and pre-systemic metabolism. Hence, the present work aims to formulate and evaluate OLZ nanoparticles dissolving microneedles (MNs) for transdermal delivery to overcome the problems associated with drug administration orally. OLZ nanoparticles were prepared by the nanoprecipitation method. The optimized OLZ nanoparticle formula was utilized for the fabrication of dissolving MNs by loading OLZ nanodispersion into polydimethylsiloxane (PDMS) micromould cavities, followed by casting the polymeric solution of polyvinylpyrrolidone(PVP-K30) and polyvinyl alcohol (PVA) to form MN matrix. The results revealed that the optimized OLZ nanoparticle formula (NP-5) exhibited particle size 115.76±5.45 nm, entrapment efficiency 78.4±5.46, and zeta potential -19.01±1.6 mV. The results of MNs revealed that MN-4 exhibits a high drug content of 98.52%, and ex vivo permeation through rabbit skin exhibited that MN-4 permeates more effectively than a simple patch by approximately 5.16 fold. In vivo pharmacokinetics study revealed that the area under curve AUC 0-∞ of MN-4 was 6054.56±376 ng. h/ml as compared with AUC0-∞ of marketed OLZ tablet was 3975.77±373 ng. h/ml. It can be concluded that the dissolving MN-4 patch is considered a promising formula to overcome the problems associated with drug administration orally and could improve drug bioavailability, in addition to the ease of administering the medication to schizophrenic patients.

Effect of Microsphere Concentration on Catechin Release from Microneedle Arrays.

In this work, microspheres were developed by cross-linking glutaraldehyde in an aqueous gelatin solution with a surfactant and solvent. A poly(vinyl alcohol) (PVA) solution was produced and combined with catechin-loaded microspheres. Different microsphere concentrations (0%, 5%, 10%, and 15%) were applied to the PVA microneedles. The moisture content, particle size, swelling, and drug release percentage of microneedles were studied using various microsphere concentrations. Fourier transform infrared and scanning electron microscopy (SEM) investigations validated the structure of gelatin microspheres as well as their decoration in microneedles. The SEM scans revealed that spherical microspheres with a wrinkled and folded morphology were created, with no physical holes visible on the surface. The gelatin microspheres generated had a mean particle size of 20-30 μm. Ex vivo release analysis indicated that microneedles containing 10% microspheres released the most catechin, with 42.9% at 12 h and 84.4% at 24 h.

Peculiarities of clabsi in oncologic hospitals

Currently, healthcare-associated infections (HAIs) are one of the most dangerous complications for hospitalized patients. The economic damage caused by HCAI in the Russian Federation annually is about 15 billion rubles; in addition, HCAI occupy the tenth place among the causes of mortality of the population. Catheter-associated bloodstream infection holds the leading place in the structure of ISMP morbidity. The concept of CVC-AIC (catheter-associated bloodstream infections or CLABSI), in turn, is included in CAIC. Purpose of the study. To analyze the current literature data of domestic and foreign authors for the years 2012-2022 concerning CAIC in patients of oncologic hospitals with CVCs, including subcutaneous central venous port catheters. Material and Methods. A review of 38 literature sources for the last 10 years was performed, including current information on catheter-associated bloodstream infections, measures to prevent them, and modern treatment approaches. Results. The studies have shown that the combination of drug resistance in microorganisms and immunity reduction in cancer patients, which occurs against the background of chemotherapy, makes them a risk group for the development of CAICs and episodes of their recurrence. Ensuring epidemiological safety of bloodstream catheterization in such patients is an important step in the prevention of CAIC. This is one of the priority tasks of oncoepidemiology today. Conclusion. The leading role in the occurrence of catheter-associated bleeding plays the term of catheterization and the condition of the patient, his age, stage of cancer development and concomitant chronic diseases. One of the most vulnerable risk groups are cancer patients from hemoblastosis chemotherapy and bone marrow and hematopoietic stem cell transplantation units. This may be due to the use of immunosuppressant drugs to suppress graft rejection, which significantly reduce patients' immunity. According to the results of studies, infection with drug-resistant Gram-negative microorganisms, including multidrug-resistant ones, is prevalent in cancer patients with CAIC. When using povidone iodine, there is a tendency to decrease the incidence of CAIC, but the use of chlorhexidine alcohol solution showed better results. A direct correlation was observed between the use of surgically implanted intravascular devices for long-term function and a lower incidence of CAIC, particularly in pediatric oncology.

High potency of linalool-zinc oxide nanocomposite as a new agent for cystic echinococcosis treatment.

This current in vitro, ex vivo, and in vivo research aims to evaluate and analyze the linalool-zinc oxide nanocomposite (Lin-ZNP) for treating cystic echinococcosis. Lin-ZNP was synthesized using an ethanolic solution of polyvinyl alcohol. The protoscolicidal effects of Lin-ZNP were tested on hydatid cyst protoscoleces (PTS) in both in vitro and ex vivo by eosin exclusion test. The study also examined the impact on caspase-3 gene expression and the external structure of PTS. The in vivo effect was measured by examining hydatid cysts' quantity, dimensions, and weight in mice intraperitoneally infected with 0.5 mL of PTS solution containing 1,000 PTS. The antioxidant and inflammatory cytokine gene expression levels were examined using real-time PCR. Lin-ZNP significantly (P < 0.001) killed the PTS in both in vitro and ex vivo in a dose- and time-dependent manner. The treated PTS exhibited creases and protrusions as a result of bleb formation and upregulation in the gene expression of caspase-3. Upon treatment with Lin-ZNP, there was a significant (P < 0.001) reduction in the number, diameter, and weight of the hydatid cysts. Treatment with Lin-ZNP nanocomposite led to a significant increase in the expression of antioxidant genes and a notable decrease in oxidative stress markers, and the expression levels of IL-4 and IL-10. Lin-ZNP has the potential to act as a scolicidal agent and demonstrates promise in controlling hydatid cysts in a mouse model, attributed to its antioxidant and anti-inflammatory properties. However, additional studies in clinical trials are needed to confirm the use of Lin-ZNP for treating hydatidosis.

Swelling of oak wood in alcoholic solutions: synergy and memory effects between water and ethanol

Swelling of oak wood in alcoholic solutions: synergy and memory effects between water and ethanol

Comparative morpho-anatomy of two sedges (&lt;i&gt;Cyperus cyperoides&lt;/i&gt;(L.) Kuntze and &lt;i&gt;Cyperus rotundus&lt;/i. L.)

The research investigated the comparative morpho-anatomy of two sedges (Cyperus cyperoides (L.) Kuntze and Cyperus rotundus L.), members of the family Cyperaceae. They are perennial herbs and the former commonly known as commonflat sedge and the latter nut sedge or purple nutsedge. The epidermal peels were obtained by standard methods. The samples were fixed in formaldehyde, glacial acetic acid, 70% alcohol in the ratio of 1:1:18, dehydrated in alcohol solutions of 50%, 70%, 90%, absolute and sectioned, stained in 2% aqueous solution of Safranin O, counter stained in Alcian blue, mounted in glycerine.The result on epidermal studies showcased both kidney and dumbbell-shaped guard cells, graminaceous stomata which is amphistomatic for both species. The anatomical studies revealed scattered vascular bundies in ground tissues of stems, bulliform cells at adaxial foliar organs. The pith sections have large pith and a single row of barrel shaped endodermis prominently pronounced. The research findings here would assist in improving upon already existing knowledge about C. cyperoides and C rotundus.

Sex differences in neuronal activation in the cortex and midbrain during quinine-adulterated alcohol intake.

Continued alcohol consumption despite negative consequences is a core symptom of alcohol use disorder. This is modeled in mice by pairing negative stimuli with alcohol, such as adulterating alcohol solution with quinine. Mice consuming alcohol under these conditions are considered to be engaging in aversion-resistant intake. Previously, we have observed sex differences in this behavior, with females more readily expressing aversion-resistant consumption. We also identified three brain regions that exhibited sex differences in neuronal activation during quinine-alcohol drinking: ventromedial prefrontal cortex (vmPFC), posterior insular cortex (PIC), and ventral tegmental area (VTA). Specifically, male mice showed increased activation in vmPFC and PIC, while females exhibited increased activation in VTA. In this study, we aimed to identify what specific type of neurons are activated in these regions during quinine-alcohol drinking. We assessed quinine-adulterated alcohol intake using the two-bottle choice procedure. We also utilized RNAscope in situ hybridization in the three brain regions that previously exhibited a sex difference to examine colocalization of Fos, glutamate, GABA, and dopamine. Females showed increased aversion-resistant alcohol consumption compared to males. We also found that males had higher colocalization of glutamate and Fos in vmPFC and PIC, while females had greater dopamine and Fos colocalization in the VTA. Collectively, these experiments suggest that glutamatergic output from the vmPFC and PIC may have a role in suppressing, and dopaminergic activity in the VTA may promote, aversion-resistant alcohol consumption. Future experiments will examine neuronal circuits that contribute to sex differences in aversion resistant consumption.

Highly Mechanical Strength, Flexible and Stretchable Wood-Based Elastomers without Chemical Cross-Linking

Wood exhibits a limited elastic deformation capacity under external forces due to its small range of elastic limit, which restricts its widespread use as an elastic material. This study presents the development of a stretchable wood-based elastomer (SWE) that is highly mechanical and flexible, achieved without the use of chemical cross-linking. Balsa wood was utilized as a raw material, which was chemically pretreated to remove the majority of the lignin and create a more abundant pore structure, while exposing the active hydroxyl groups on the cellulose surface. The polyvinyl alcohol (PVA) solution was impregnated into delignified wood, resulting in the formation of a cross-linked structure through multiple freeze–thaw cycles. After eight cycles, the tensile strength in the longitudinal direction reached up to 25.68 MPa with a strain of ~463%. This excellent mechanical strength is superior to that of most wood-based elastomers reported to date. The SWE can also perform complex deformations such as winding and knotting, and SWE soaked in salt solution exhibits excellent sensing characteristics and can be used to detect human finger bending. Stretchable wood-based elastomers with high mechanical strength and toughness have potential future applications in biomedicine, flexible electronics, and other fields.

Dual-responsive solvatochromic sensor for simultaneous detection and mechanism of Cr(III) and Co(II) cations by a new N2O4-donor tetrabenzodiaza-crown ether Macrocyclic ligand anchored to dipyridine moieties

The present study describes the chemosensing capability of an N2O4-donor tetrabenzodiaza-crown ether macrocyclic ligand derivatized by two pyridine side arms to afford the flexible macrocyclic ligand, 5,20-bis(pyridin-4-ylmethyl)-5,6,12,13,19,20,26,27octahydrotetrabenzo [e,i,o,s][1,4,11,14]tetraoxa[7,18]diazacycloicosine (PMHBOA). A selective colorimetric behavior of PMHBOA for the prompt detection of Cr(ΙΙΙ) and Co(ΙΙ) can be observed with naked eyes. The 1H, 13C{H} NMR, and infrared spectroscopy disclosed the ligand binding capability for selective metal ions, ascribed to a 1:1 complex formation with Cr(III) and Co(II) cations in isopropanol. The limit of detection (LOD) values were calculated as 580 and 850 nM for Cr(ΙΙΙ) and Co(ΙΙ), respectively, accompanied with suitable LOQs as 1.95 µM for Cr(III) and 2.85 µM for Co(II), respectively. The reusability was confirmed in the presence of ethylenediaminetetraacetic acid (EDTA). Moreover, a TLC-based fabricated paper strip successfully applied PMHBOA as a chemosensor for synchronous visible detection of Cr(III) and Co(II) in their alcoholic solution. Meanwhile, the observed color change along with the turn-off fluorescent of PMHBOA guaranteed a felicitous choice of candidate for Cr(III) in the presence of other library metal ions in isopropanol:H2O (9/1 v/v) solution. A fast separation test on the filter paper, impregnated with PMHBOA, was also employed to separate Cr(III) and Co(II) cations from aqueous solutions by recording UV–vis spectra. The 1H NMR spectroscopy supported the macrocycle ring and side arms as the parallel coordination site of PMHBOA with Cr(III) and Co(II).

A transparent superhydrophobic coating on wood-based panel surfaces

ABSTRACT A transparent, environmentally friendly and durable superhydrophobic coating preparation process was developed to effectively isolate water molecules in the air, aiming to reduce the use limitations of the wood-based panel substrate and to realize its improved performance in terms of water resistance, stain resistance and durability. This study employs a coating method to apply a silica sol-modified nanocellulose (CNF) and polyvinyl alcohol (PVA) solution onto a wood-based panel substrate, creating a micro-nano rough structure. Subsequently, the vapor deposition method was employed to functionalize the silanol groups on the coating surface, resulting in the formation of fluorinated alkyl groups and achieving transparent superhydrophobic wood-based panels. The water contact angle measures 152.6°, the rolling angle was 4.5°, and the coating exhibits a light transmittance of 92%. Notably, the superhydrophobicity of the coating persists even after repeated polishing paper abrasion, demonstrating excellent anti-pollution self-cleaning properties. This study is of great significance for the long-term use of wood-based panels indoors and outdoors, as well as their application in complex and high humidity environments.

Structural Analyses of Polysaccharides Extracted from Cyanobacterial Extracellular Gels and Oriented Liquid Crystalline Microfiber Processing by Poly(vinyl alcohol)-Assisted Electrospinning.

Sacran is a supergiant cyanobacterial polysaccharide that forms mesogenic supercoil rods that exhibit liquid crystalline (LC) gels at deficient concentrations of around 0.5 wt%, and has several bioactive stimuli-responsive functions. Here, we attempted to form oriented microfibers of sacran by electrospinning, following structural analyses of the sacran rods. A heterogeneous acid-hydrolysis method using a protonated cation-exchange resin was adopted to examine the short-time exposition of concentrated acid to sacran rods. From the supernatant, the oligomeric fraction that was soluble in water and methanol was isolated. The oligomeric fraction had a main sugar ratio of α-Glc:β-Glc:α-Xyl:β-Xyl:α-Rha of 2:5:1.5:1.5:4 (Glc:Xyl:Rha = 7 (=4 + 3):3:4), and it was speculated that the sacran structure includes rhamnoglucan and xyloglucan (4:3), which are generally rigid enough to exhibit LC. To make oriented microfibers of LC sacran, solubility testing was performed on sacran to find good new solvents of polyhydroxy alcohols such as ethylene glycol, 1,2-propanediol, and glycerol. The oriented film was prepared from a sacran aqueous solution where calcium compound particles deposited on the film are different from polyhydroxy alcohol solutions. Although sacran could not form microfibers by itself, polymer composite microfibers of sacran with poly(vinyl alcohol) were prepared by electrospinning. Cross-polarizing microscopy revealed the molecular orientation of the microfibers.

Adolescent alcohol binge drinking and withdrawal: behavioural, brain GFAP-positive astrocytes and acute methamphetamine effects in adult female rats

RationaleAlcopop beverages are generally the first alcoholic beverage that young females drink which contain high levels of sugar and alcohol. The over-consumption of these drinks may encourage alcohol co-administration with methamphetamine (METH) impacting on drinking behaviour and glial function.AimsThe aims of this study were to evaluate the effect of adolescent binge alcohol exposure on consumption level, anxiety-like behaviour, cross-sensitization with METH and on astrocyte expression in reward related brain regions.MethodsAdolescent female Sprague-Dawley rats had daily 1-hour oral alcohol consumption of alcopop (ALCP; with sucrose) or ethanol-only (ETOH; without sucrose), transitioned from 5 to 15% (v/v) ethanol content for 34 days. Water and sucrose groups act as controls. During alcohol withdrawal, rats were tested for anxiety on the elevated plus maze (EPM) and locomotor activity following saline or METH (1 mg/kg i.p) treatment. Brains were then collected to assess astrocyte immunofluorescence for glial fibrillary acidic protein (GFAP) in reward-related brain regions.ResultsRats pretreated with 5% ALCP consumed significantly more volume and ethanol intake when compared to 5% EtOH rats. Both ALCP and EtOH groups had a higher preference ratio for 5% than 15% alcohol solutions and ALCP rats had greater ethanol intake at 15% than EtOH rats. Alcohol withdrawal showed no significant differences between groups on anxiety, METH cross-sensitization effects or GFAP intensity in the regions studied.ConclusionsOverall, the addition of sucrose to alcoholic solutions encouraged female rats to consume larger volumes and greater ethanol intake compared to ethanol-only solutions, yet did not have long lasting effects on behaviour and astrocytes.

The study of marketing opportunities for the use of the raw material of feverfew (Tanacetum parthenium L.) in the pharmacotherapy of musculoskeletal system diseases

Aim. To form a modern idea of the market structure and trends in the consumption of drugs for the pharmacotherapy of rheumatoid arthritis, to determine the possible potential for use of plant raw material of feverfew (Tanacetum parthenium). Materials and methods. The study was conducted using the structural analysis, logical and graphical methods, and marketing analysis methods. Results. It was found that in the pre-war period the market for drugs affecting the musculoskeletal system developed quite vigorously – its growth rate in the period of 2020-2021 ranged from 16 to 29 % in natural units and from 20 to 30 % in monetary terms. According to the structure of producing countries, the market was significantly import-dependent. The market for synthetic drugs was quite saturated, represented by various dosage forms – tablets, powder for suspension, injection solutions (ampoules), granules, capsules, sachets, alcohol solutions, oil solutions, suppositories, oral suspensions, transdermal patches, gels, ointments. The research was conducted taking into account the study of marketing opportunities for the use of the raw material of Tanacetum parthenium in the pharmacotherapy of diseases of the musculoskeletal system since this raw material had certain advantages compared to synthetic analogs. It should be noted that the market of herbal medicines for the treatment of the musculoskeletal system diseases or the prevention of their manifestations is very limited and is represented by a minimum number of product items and one representative of the medicinal flora – Symphytum officinale. In addition, 90 % of the market for this group of drugs belongs to foreign manufacturers. Although research of the market for biological supplements indicates a certain demand for products based on the medicinal plant raw material. Conclusions. Based on the trends identified, we can state the positive development of the market for drugs that affect the musculoskeletal system, the prospects for the development of drugs based on the medicinal plant raw material, including Tanacetum parthenium.

Peculiarities of Modification of Amorphous Alloys Surface with Oligomer Coatings from Alcohol Solutions

Peculiarities of Modification of Amorphous Alloys Surface with Oligomer Coatings from Alcohol Solutions

Linalool-zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity.

The present in vitro and in vivo study aimed to fabricate and characterize linalool-zinc oxide nanoparticles (Lin-ZNP) and evaluate their effectiveness against Toxoplasma gondii infection in terms of inflammation, oxidative stress, and pathogenicity. Lin-ZNP was synthesized using an ethanolic solution of polyvinyl alcohol. The anti-Toxoplasma and cytotoxicity activities of Lin-ZNP were investigated, along with its effects on nitric oxide (NO) production, caspase-3 activity, and pro-inflammatory genes. After treating T. gondii-infected mice with Lin-ZNP for 14 days, the number and size of tissue cysts, antioxidant potential, pro-inflammatory cytokines, and T. gondii pathogenicity-related genes were evaluated by real-time polymerase chain reaction and Western blot analysis. The Lin-ZNP composite showed a reduced tendency with an average size of 105 nm. Lin-ZNP significantly reduced the viability of tachyzoites. The obtained selectivity index higher than 10, indicating high specificity for parasites with low cytotoxicity to normal cells. The Lin-ZNP significantly (p < 0.05) increased the production of NO, caspase-3 activity, and the expression levels of pro-inflammatory genes. Lin-ZNP significantly (p < 0.001) decreased the size and number of tissue cysts and caused a significant reduction in the level of malondialdehyde and a considerable increase (p < 0.001) in antioxidant enzymes and their expression genes. Lin-ZNP significantly downregulated both mRNA and protein expression of the inflammation-related markers associated with the TLRs/NF-κB pathway. The expression levels of the T. gondii pathogenicity-related genes were significantly downregulated (p < 0.05). The recent survey indicated that Lin-ZNP manages T. gondii infection by its antioxidant activity and inhibiting the TLRs/NF-κB pathway without toxicity in mice.