Airborne Microbiota Research Articles

Metagenomic Analysis of Microbial Diversity in the Urban Air of Sofia (Bulgaria) during Spring and Winter

The main scientific interest for decades remained focused primarily on physical and chemical air pollutants, which severely limited our knowledge of microbial diversity in the atmosphere. The ecology of airborne microorganisms is poorly understood, even though they are a constant element of the air we breathe. Their exploration is very important for understanding health and ecological impacts, mainly in the highly urbanized cities. The main purpose of this study is to explore the biodiversity of microorganisms in the atmosphere over the densely populated centre of Sofia (Bulgaria) during spring and winter by Next-generation sequencing. The results showed that the most prevailing bacteria in the urban bioaerosols were representatives of phyla Firmicutes, Proteobacteria, Bacteroides, Actinobacteria. Many of the identified species were soil and leaf surface dwellers. Most of the detected fungal sequences belong to the phylum Ascomycota. Some pathogenic bacterial and fungal taxa were also found. This study provides the first information about microbial diversity in the outdoor air above Sofia and contributes to better understanding of urban airborne microbiota.

Neighborhood plant community, airborne microbiota transferred indoors and prevalence of respiratory diseases are interrelated: A cross-sectional study

Airborne microbiota transferred indoors (AMTI) is linked to human respiratory health. Yet, the factors influencing these microorganisms and their connections to the prevalence of respiratory diseases (RDs) remain unclear. In this study, we examined plant communities and AMTI using VenTube, next-generation sequencing and quantitative polymerase chain reaction (qPCR) in 72 Shanghai neighborhoods in warm and cold seasons, respectively. To determine the prevalence of RDs, we collected 1026 questionnaires, enlisting 30 ± 5 volunteers aged 40–80, residing in the area for more than a decade, with an equal gender balance. Our results demonstrated that the AMTI communities were less diverse in the cold season than in the warm season, which is in agreement with the changes of garden plant diversity between seasons. Along the reduction of AMTI diversity, greater relative abundances of RDs-associated microbes (e.g., Pseudomonas and Streptococcus) was transferred indoors during the cold season. The questionnaire survey showed that the most prevalent symptom was shortness of breath (25.6 %), followed by rhinitis (20.8 %) and wheeze (14.4 %), with generally no prevalence difference between urban and peri-urban neighborhoods. Notably, despite the sparse garden plant community in the cold season, the abundance of Oleaceae trees showed an inverse relationship with the RDs-associated microbes as well as the prevalences of RDs based on the structural equation model results. This finding was largely supported by the negative effect of Oleaceae trees on the population of Streptococcus anginosus (qPCR) which was a dominant species transferred indoors in the cold season, given that S. anginosus is highly associated with rhinitis and rhinoconjunctivitis. Taken together, our findings suggest a strong association between the Oleaceae trees, the AMTI and the prevalence of RDs, which can shed some lights in the ecological development towards respiratory safe environment in cities.

Indoor-outdoor microbial exchange: Quantifying the contribution of outdoor sources to indoor microbiota across seasons

BackgroundBacteria and fungi are ubiquitous, suspended in the air, and have the potential to influence human health. However, the relationship between microbes in outdoor air, soil, and indoor air remains unknown. MethodsThis study comprehensively analyzed the microbes of indoor air, outdoor air and soil during summer and winter in Nanjing, a hot summer and cold winter city in China. A total of 102 rooms, 544 culturable samples, and 406 sequencing samples were investigated. The Sloan neutral model (SNM) and the fast expectation-maximization microbial source tracking (FEAST) were used to analyze the relationship between indoor and outdoor microbial communities. ResultsThe impact of season on bacterial diversity differed from that on fungal diversity. Microbial diversities and community structures in indoor air, outdoor air, and soil were similar within the same season but differed between seasons. The fitting results of the SNM indicated that the stochastic assembly processes of both indoor and outdoor airborne bacteria were greater in summer than in winter, whereas for airborne fungi, the situation was reversed. The FEAST calculating results suggested that outdoor air serves as a more significant source than soil for indoor air microbiota both in summer and winter. ConclusionThis study provided a new insight into understanding the quantitative contributions of outdoor sources to indoor airborne microbiota. Future studies should include a wider range of building types and extend across multiple seasons to provide deeper insights into the quantitative relationship between indoor and outdoor microbiota.

Effects of Speleotherapy on Aerobiota: A Case Study from the Sežana Hospital Cave, Slovenia

Speleotherapy is one of the non-pharmacological methods for the treatment and rehabilitation of patients with chronic respiratory diseases, especially those with chronic obstructive pulmonary disease (COPD) and asthma. On the one hand, one of the alleged main advantages of speleotherapeutic caves is the low microbial load in the air and the absence of other aeroallergens, but on the other hand, due to the lack of comprehensive air monitoring, there is little information on the pristine and human-influenced aerobiota in such environments. The aim of this study was to assess the anthropogenic effects of speleotherapy on the air microbiota and to investigate its potential impact on human health in Sežana Hospital Cave (Slovenia). From May 2020 to January 2023, air samples were collected in the cave before and after speleotherapeutic activities using two different volumetric air sampling methods—impaction and impingement—to isolate airborne microbiota. Along with sampling, environmental data were measured (CO2, humidity, wind, and temperature) to explore the anthropogenic effects on the aerobiota. While the presence of patients increased microbial concentrations by at least 83.3%, other parameters exhibited a lower impact or were attributed to seasonal changes. The structure and dynamics of the airborne microbiota are similar to those in show caves, indicating anthropization of the cave. Locally, concentrations of culturable microorganisms above 1000 CFU/m3 were detected, which could have negative or unpredictable effects on the autochthonous microbiota and possibly on human health. A mixture of bacteria and fungi typically associated with human microbiota was found in the air and identified by MALDI-TOF MS with a 90.9% identification success rate. Micrococcus luteus, Kocuria rosea, Staphylococcus hominis, and Staphylococcus capitis were identified as reliable indicators of cave anthropization.

Studies on airborne microbiota in Mexico, a review

Airborne microbiota has garnered increasing interest in recent decades, not only because of its role as carrier of pathogenic microorganisms and its involvement in the deterioration of man-made objects, buildings, and food but also because of its participation in atmospheric processes and its content of microorganisms, important for biogeochemical cycles in different ecosystems. In order to understand and comprehensively analyze existing studies on airborne microbiota in Mexico, a systematic review was conducted utilizing Web of Science and Google Scholar as search tools. The information found in the 35 identified publications was systematically organized, including title, study location, sampling method, laboratory approach, identification method, and organisms found. This information was then ordered into three sections: bibliometric analysis, employed methodologies, and main findings. Through this work, it was revealed that the studies were carried out in 14 out of the 32 states of Mexico, with Mexico City standing out with 17 articles. Three methodological approaches were identified: microbial cultures (77% of the studies), microscopy (11.5%), and metagenomics (11.5%). The studies predominantly focused on state capitals or densely populated cities, making the identification of potentially pathogenic organisms from humans, animals, and plants, of particular interest. This comprehensive review lays the foundation for proposing compelling avenues of future research, aimed at advancing our comprehension of airborne microbiota in Mexico.

Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

Over the last few years, the cumulative use of antibiotics in healthcare institutions, as well as the rearing of livestock and poultry, has resulted in the accumulation of antibiotic resistance genes (ARGs). This presents a substantial danger to human health worldwide. The characteristics of airborne ARGs, especially those transferred from outdoors to indoors, remains largely unexplored in neighborhoods, even though a majority of human population spends most of their time there. We investigated airborne ARGs and mobile genetic element (MGE, IntI1), plant communities, and airborne microbiota transferred indoors, as well as respiratory disease (RD) prevalence using a combination of metabarcode sequencing, real-time quantitative PCR and questionnaires in 72 neighborhoods in Shanghai. We hypothesized that (i) urbanization regulates ARGs abundance, (ii) the urbanization effect on ARGs varies seasonally, and (iii) land use types are associated with ARGs abundance. Supporting these hypotheses, during the warm season, the abundance of ARGs in peri-urban areas was higher than in urban areas. The abundance of ARGs was also affected by the surrounding land use and plant communities: an increase in the proportion of gray infrastructure (e.g., residential area) around neighborhoods can lead to an increase in some ARGs (mecA, qnrA, ermB and mexD). Additionally, there were variations observed in the relationship between ARGs and bacterial genera in different seasons. Specifically, Stenotrophomonas and Campylobacter were positively correlated with vanA during warm seasons, whereas Pseudomonas, Bacteroides, Treponema and Stenotrophomonas positively correlated with tetX in the cold season. Interstingly, a noteworthy positive correlation was observed between the abundance of vanA and the occurrence of both rhinitis and rhinoconjunctivitis. Taken together, our study underlines the importance of urbanization and season in controlling the indoor transfer of airborne ARGs. Furthermore, we also highlight the augmentation of green–blue infrastructure in urban environments has the potential to mitigate an excess of ARGs.

From air to airway: Dynamics and risk of inhalable bacteria in municipal solid waste treatment systems

Municipal solid waste treatment (MSWT) system emits a cocktail of microorganisms that jeopardize environmental and public health. However, the dynamics and risks of airborne microbiota associated with MSWT are poorly understood. Here, we analyzed the bacterial community of inhalable air particulates (PM10, n = 71) and the potentially exposed on-site workers’ throat swabs (n = 30) along with waste treatment chain in Shanghai, the largest city of China. Overall, the airborne bacteria varied largely in composition and abundance during the treatment (P < 0.05), especially in winter. Compared to the air conditions, MSWT-sources that contributed to 15 ∼ 70% of airborne bacteria more heavily influenced the PM10-laden bacterial communities (PLS-SEM, β = 0.40, P < 0.05). Moreover, our year-span analysis found PM10 as an important media spreading pathogens (104 ∼ 108 copies/day) into on-site workers. The machine-learning identified Lactobacillus and Streptococcus as pharynx-niched featured biomarker in summer and Rhodococcus and Capnocytophaga in winter (RandomForest, ntree = 500, mtry = 10, cross = 10, OOB = 0%), which closely related to their airborne counterparts (Procrustes test, P < 0.05), suggesting that MSWT a dynamic hotspot of airborne bacteria with the pronounced inhalable risks to the neighboring communities.

Composition Analysis of Airborne Microbiota in Outdoor and Indoor Based on Dust Separated by Micro-sized and Nano-sized

Composition Analysis of Airborne Microbiota in Outdoor and Indoor Based on Dust Separated by Micro-sized and Nano-sized

Abundant bacteria and fungi attached to airborne particulates in vegetable plastic greenhouses

The proliferation of modern vegetable plastic greenhouses (VPGS) supplies more and more vegetables for food all over the world. The airborne bacteria and fungi induce more exposure opportunities for workers toiling in confined plastic greenhouses. Culture-independent approaches by qPCR and high-throughput sequencing technology were used to study the airborne particulates microbiota in typic VPGS in Shandong, a large base of vegetables in China. The result revealed the mean airborne bacteria concentrations reached 1.67 × 103 cells/m3 (PM2.5) and 2.38 × 103 cells/m3 (PM10), and the mean airborne fungal concentrations achieved 1.49 × 102 cells/m3 (PM2.5) and 3.19 × 102 cells/m3 (PM10) in VPGS. The predominant bacteria in VPGS included Ralstonia, Alcanivorax, Pseudomonas, Bacillus, and Acinetobacter. Botrytis, Alternaria, Fusarium, Sporobolomyces, and Cladosporium were frequently detected fungal genera in VPGS. A higher Chao1 of bacteria in PM10 was significantly different from PM2.5 in VPGS. The potential pathogens in VPGS include Raltonia picketti, Acinetobacter lwoffii, Bacillus anthracis, Botrytis cinerea, and Cladosporium sphaerospermum. The network analysis indicated that airborne microbiota was associated with soil microbiota which was affected by anthropologic activities. The predicted gene functions revealed that bacterial function mainly involved metabolism, neurodegenerative diseases, and fungal trophic mode dominated by Pathotroph-Saprotroph in VPGS. These findings unveiled airborne microbiomes in VPGS so that a strategy for improving air quality can be applied to safeguard health and vegetation.

City-Scale Meta-Analysis of Indoor Airborne Microbiota Reveals that Taxonomic and Functional Compositions Vary with Building Types.

Currently, there is a lack of understanding on the variations of the indoor airborne microbiotas of different building types within a city, and how operational taxonomic unit (OTU)- and amplicon sequence variant (ASV)-based analyses of the 16S rRNA gene sequences affect interpretation of the indoor airborne microbiota results. Therefore, in this study, the indoor airborne bacterial microbiotas between commercial buildings, residences, and subways within the same city were compared using both OTU- and ASV-based analytic methods. Our findings suggested that indoor airborne bacterial microbiota compositions were significantly different between building types regardless of the bioinformatics method used. The processes of ecological drift and random dispersal consistently played significant roles in the assembly of the indoor microbiota across building types. Abundant taxa tended to be more centralized in the correlation network of each building type, highlighting their importance. Taxonomic changes between the microbiotas of different building types were also linked to changes in their inferred metabolic function capabilities. Overall, the results imply that customized strategies are necessary to manage indoor airborne bacterial microbiotas for each building type or even within each specific building.

General decline in the diversity of the airborne microbiota under future climatic scenarios

Microorganisms attached to aerosols can travel intercontinental distances, survive, and further colonize remote environments. Airborne microbes are influenced by environmental and climatic patterns that are predicted to change in the near future, with unknown consequences. We developed a new predictive method that dynamically addressed the temporal evolution of biodiversity in response to environmental covariates, linked to future climatic scenarios of the IPCC (AR5). We fitted these models against a 7-year monitoring of airborne microbes, collected in wet depositions. We found that Bacteria were more influenced by climatic variables than by aerosols sources, while the opposite was detected for Eukarya. Also, model simulations showed a general decline in bacterial richness, idiosyncratic responses of Eukarya, and changes in seasonality, with higher intensity within the worst-case climatic scenario (RCP 8.5). Additionally, the model predicted lower richness for airborne potential eukaryotic (fungi) pathogens of plants and humans. Our work pioneers on the potential effects of environmental variability on the airborne microbiome under the uncertain context of climate change.

A long-term atmospheric baseline for intercontinental exchange of airborne pathogens

The atmosphere is a potential pathway for global-scale and long-range dispersal of viable microorganisms, promoting biological interconnections among the total environment. We aimed to provide relevant baseline information for long-range long-term intercontinental exchange of potentially infectious airborne microorganisms of major interest in environmental and health-related disciplines. We used an interannual survey (7-y) with wet depositions fortnightly collected above the boundary layer (free troposphere) at a remote high-elevation LTER (Long-Term-Ecological-Research) site, analyzed by 16S and 18S rRNA genes, and compared to a database of 475 well-known pathogens. We applied a conservative approach on close relatives of pathogenic species (>98% identity) standing their theoretical upper limit for atmospheric baseline relative abundances. We identified c. 2–3% of the total airborne microbiota as potential pathogens. Their most frequent environmental origins were soil, aquatic, and anthropogenic sources. Phytopathogens (mostly fungi) were the potential infectious agents most widely present. We uncovered consistent interannual dynamics with taxa foreseeable over time (i.e., predictable seasonal behavior) and under recurrent environmental scenarios (e.g., Saharan dust intrusions), respectively, being highly valuable microbial forensic environmental indicators. Up to 8 bacterial and 21 fungal genera consistently showed temporal abundances and recurrences unevenly distributed. Incidence of allergenic fungi was lower in summer, and significantly higher in spring. Close relatives to Coccidioides posadasii consistently showed higher signals (i.e., high specificity and high fidelity) in winter, whereas Cryptococcus neoformans had a significant signal in spring. Along Saharan dust intrusions, the bacterial phytopathogens Acidovorax avenae and Agrobacterium tumefaciens and the fungal phytopathogens Pseudozyma hubeiensis and Peniophora sp. consistently showed higher signals. Potential human pathogens showed low proportion, being mostly fungal allergens. Microorganisms related to obligated human, amphibian and fish pathogens were commonly found in winter. More studies in remote field sites above the boundary layer will unveil whether or not a similar trend is found globally.

Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings

This study aims to assess the airborne bioburden of rural and urban Portuguese Primary Health Care Centers (PHCC) using active and passive sampling methods and identify the potential differences in airborne microbiota between both environments. The highest total aerobic mesophilic bacterial load in indoor air were found in the Vaccination Room (448 CFU.m−3) in the Rural PHCC and in the Waiting Room (420 CFU.m−3) for Urban PHCC. The total coliforms contamination level in indoor air was detected only in the Cleaning Supplies Room (4 CFU.m−3) in the Urban PHCC. The most frequent bacteria genera identified was Micrococcus (21% Rural PHCC; 31% Urban PHCC). The surface samples showed a highest total aerobic mesophilic bacterial contamination in the Treatment Room (86 × 103 CFU.m−2) from the Rural PHCC and in the Front Office (200 × 103 CFU.m−2) from the Urban PHCC. The electrostatic dust cloth (EDC) samples showed a highest bacterial load in the Urban PHCC. Total aerobic mesophilic bacterial load in settled dust and in the Heating, Ventilating and Air Conditioning (HVAC) filter samples in the Urban PHCC (8 CFU.g−1 and 6 × 103 CFU.m−2) presented higher values compared with the Rural PHCC (1 CFU.g−1 and 2.5 × 103 CFU.m−2). Urban PHCC presented higher bacterial airborne contamination compared with the Rural PHCC for the majority of sampling sites, and when compared with the indoor air quality (IAQ) Portuguese legislation it was the Rural PHCC in two sampling places who did not comply with the established criteria.

Influence of pig farming on human Gut Microbiota: role of airborne microbial communities

ABSTRACT It has been hypothesized that both genetics and diet influence the composition of the human cecal microbiota. However, it remains unclear whether and how occupational exposure to microbes impacts the microbial communities in human guts. Using a One Health approach, we visited pig farms (n = 26) and collected stool specimens from pig workers (n = 59), pig barn air samples (n = 19), and rectal swabs from pigs at three different growth stages (n = 144). Stool samples from cattle workers were included as a control group (n = 22). Each sample’s microbiota was characterized using 16S rRNA gene sequencing and the DADA2 pipeline. We obtained a significantly different clustering of the microbial compositions of pig and cattle workers by permutational multivariate analysis of variance (PERMANOVA; P < .001). Workers primarily exposed to pigs had higher relative abundances of Prevotellaceae and less Bacteroidaceae than workers exposed to cattle. We also found that the microbial compositions of pig workers’ stool samples shared extensive fractions with the samples from their pigs. We also identified amplicon sequencing variants (ASVs) in the airborne microbiota which were likely involved in zoonotic transmission events. We hypothesize that ASVs originating from pig feces are aerosolized and, through breathing, get trapped in the pig farm workers’ upper respiratory tract from where they can get swallowed. Consequently, some of the animal associated ASVs are transferred into the gastrointestinal tracts (GITs) which leads to changes in the composition of the human gut microbiota. The importance of this finding for human health must be investigated further.

Microbiological indoor air quality in faculty's rooms: Risks on students' health

This study deals with a quantitative and qualitative analysis of indoor airborne microbiota and estimation of microbiological quality of indoor air in faculty's rooms during the summer semester of 2017/18 school year. The concentration of bacteria was significantly higher than the concentration of fungi. The species that belong to human skin microbiota or of environmental origin were identified. According to indoor air quality breakpoints, low to medium/high level of bacterial and fungal air contaminations was noticed.

Profiling Airborne Microbiota in Mechanically Ventilated Buildings Across Seasons in Hong Kong Reveals Higher Metabolic Activity in Low-Abundance Bacteria.

Metabolically active bacteria within built environments are poorly understood. This study aims to investigate the active airborne bacterial microbiota and compare the total and active microbiota in eight mechanically ventilated buildings over four consecutive seasons using the 16S rRNA gene (rDNA) and the 16S rRNA (rRNA), respectively. The relative abundances of the taxa of presumptive occupants and environmental origins were significantly different between the active and total microbiota. The Sloan neutral model suggested that ecological drift and random dispersal played a smaller role in the assembly of the active microbiota than the total microbiota. The seasonal nature of the active microbiota was consistent with that of the total microbiota in both indoor and outdoor environments, while only the indoor environment was significantly affected by geography. The relative abundances of the active and total taxa were positively correlated, suggesting that the high-abundance members were also the greatest contributors to the community-level metabolic activity. Based on the rRNA/rDNA ratio, the low-abundance members consistently had a higher taxon-level metabolic activity than the high-abundance members over seasons, suggesting that the low-abundance members may have the ability to survive and thrive in the indoor environment and their impact on the health of occupants cannot be overlooked.

Factors affecting the exposure to physicochemical and microbiological pollutants in vehicle cabins while commuting in Lisbon.

Commuters are exposed to a variety of physicochemical and microbiological pollutants that can lead to adverse health effects. This study aims to evaluate the indoor air quality (IAQ) in cars, buses and trains in Lisbon, to estimate inhaled doses while commuting and to evaluate the impacts of cleaning and ventilation on the IAQ. Particulate matter with diameter lower than 1, 2.5 and 10μm (PM1, PM2.5 and PM10), black carbon (BC), carbon monoxide (CO), carbon dioxide (CO2) volatile organic compounds (VOCs), formaldehyde (CH2O) and total airborne bacteria and fungi were measured and bacterial isolates were identified. Results showed that the type of ventilation is the main factor affecting the IAQ in vehicle cabins. Under the fan off condition, the concentration of BC was lower, but the concentration of gases such as CO2, CO and VOC tended to accumulate rapidly. When the ventilation was used, the coarse particles were filtered originating the decrease of indoor concentrations. Commuters travelling in trains received the lowest dose for all chemical pollutants, except VOC, mainly because railways are further away from the direct vehicular emissions. Commuters travelling in cars without ventilation received the highest inhaled dose for almost all pollutants despite having the lowest travel duration. Airborne microbiota was highly affected by the occupancy of the vehicles and therefore, the fungi and bacterial loads were higher in trains and buses. Most of the isolated species were human associated bacteria and some of the most abundant species have been linked to respiratory tract infections.

Vertical Stratification in Urban Green Space Aerobiomes.

Background:Exposure to a diverse environmental microbiome is thought to play an important role in “educating” the immune system and facilitating competitive exclusion of pathogens to maintain human health. Vegetation and soil are key sources of airborne microbiota––the aerobiome. A limited number of studies have attempted to characterize the dynamics of near surface green space aerobiomes, and no studies to date have investigated these dynamics from a vertical perspective. Vertical stratification in the aerobiome could have important implications for public health and for the design, engineering, and management of urban green spaces.Objectives:The primary objectives of this study were to: a) assess whether significant vertical stratification in bacterial species richness and evenness (alpha diversity) of the aerobiome occurred in a parkland habitat in Adelaide, South Australia; b) assess whether significant compositional differences (beta diversity) between sampling heights occurred; and c) to preliminarily assess whether there were significant altitudinal differences in potentially pathogenic and beneficial bacterial taxa.Methods:We combined an innovative columnar sampling method at soil level, 0.0, 0.5, 1.0, and , using passive petri dish sampling to collect airborne bacteria. We used a geographic information system (GIS) to select study sites, and we used high-throughput sequencing of the bacterial 16S rRNA gene to assess whether significant vertical stratification of the aerobiome occurred.Results:Our results provide evidence of vertical stratification in both alpha and beta (compositional) diversity of airborne bacterial communities, with diversity decreasing roughly with height. We also found significant vertical stratification in potentially pathogenic and beneficial bacterial taxa.Discussion:Although additional research is needed, our preliminary findings point to potentially different exposure attributes that may be contingent on human height and activity type. Our results lay the foundations for further research into the vertical characteristics of urban green space aerobiomes and their implications for public health and urban planning. https://doi.org/10.1289/EHP7807

Microbial emission levels and diversities from different land use types

Bioaerosol particles, originated from many different earth ground sources, have unique health impacts, including respiratory infections, allergic reactions, and toxic effects. Here, we applied a portable high-flow sampler HighBioTrap to collect and investigate bioaerosol emissions from 13 different land types (forest, wetland, lake, bare soil, cropland, wastewater treatment facility, street, livestock farm, smeltery and garden) that are heavily or less affected by humans. Plate cultivation, real-time quantitative PCR analysis (q-PCR) and high-throughput gene sequencing analysis were used to characterize bacterial and fungal levels as well as their community structures emitted from different land use types. Results showed that there were statistically significant differences in biological emission levels (up to 100-fold difference) and diversity among different land use types. Cropland, sewage plant street and smeltery heavily affected by human activities were found to exhibit higher bioaerosol emission levels, with Massilia genus detected as the dominant species. In contrast, some land types (lakes, forests, gardens, and wetland) less affected by humans were found to emit lower bioaerosol levels but with higher culturability, e.g., up to 16% for wetland. In addition, the microbiological structures of these land-use types usually had higher species richness and diversity, yet different dominant species. For some land types such as streets in Beijing, the microbial community appeared to be skewed with an over 80% relative abundance of a specific dominant species such as Massilia. Other detected dominant species also included Acinetobacter and Brevundimonas for street, and Sphingomonas for wetland. For fungal community, Naganishia, Alternaria, Penicillium, and Aureobasidium were detected to be most abundant. RDA analysis showed metals and ions could to some extent affect the microbial community structures. This work highlights that the human activities could substantially affect the airborne microbiota, which in turn could affect local human health and ecosystems. On the other hand, the results here provide important references for quantitatively estimating the microbial emissions from the earth into the atmosphere.

Naturally-diverse airborne environmental microbial exposures modulate the gut microbiome and may provide anxiolytic benefits in mice

Growing epidemiological evidence links natural green space exposure with a range of health benefits, including for mental health. Conversely, greater urbanisation associates with increased risk of mental health disorders. Microbiomes are proposed as an important but understudied link that may help explain many green space-human health associations. However, there remains a lack of controlled experimental evidence testing possible beneficial effects from passive exposure to natural biodiversity via airborne microbiota. Previous mouse model studies have used unrealistic environmental microbial exposures—including excessive soil and organic matter contact, feed supplements and injections—to demonstrate host microbiota, immune biomarker, and behavioural changes. Here, in a randomised controlled experiment, we demonstrate that realistic exposures to trace-level dust from a high biodiversity soil can change mouse gut microbiota, in comparison to dust from low biodiversity soil or no soil (control) (n = 54 total mice, comprising 3 treatments × 18 mice, with 9 females + 9 males per group). Furthermore, we found a nominal soil-derived anaerobic spore-forming butyrate-producer, Kineothrix alysoides, was supplemented to a greater extent in the gut microbiomes of high biodiversity treatment mice. Also, increasing relative abundance of this rare organism correlated with reduced anxiety-like behaviour in the most anxious mice. Our results point to an intriguing new hypothesis: that biodiverse soils may represent an important supplementary source of butyrate-producing bacteria capable of resupplying the mammalian gut microbiome, with potential for gut health and mental health benefits. Our findings have potential to inform cost-effective population health interventions through microbiome-conscious green space design and, ultimately, the mainstreaming of biodiversity into health care.