The shoot and root growth not only serve as important indicators for measuring the quality of tea cutting seedlings, but also can provide scientific basis for agricultural production and fine seedling management. Currently, manual weighing is the main method used to measure shoot and root growth, which is both destructive and inefficient. The study proposes a method for monitoring the shoot and root growth in tea cutting seedlings using hyperspectral imaging. First, mature leaves and shoots spectra were extracted from tea cutting seedlings using Mask R-CNN (Epoch=20 and Learning-rate=0.001). The spectra were then preprocessed by MSC, S-G, 1-D filtering techniques, with feature bands screened by UVE (Optimal factor number=5), CARS (Monte Carlo sampling times=300), and SPA (Epoch=25). Finally, a CNN-GRU (Epoch=100 and Learning-rate=0.01) network was employed to predict shoot and root biomass, and compared with machine learning methods of SVR (Kernel function=Polynomial), RFR (Ntrees=200), and PLSR (Latent variable=16) and deep learning methods (Epoch=100 and Learning-rate=0.01) of CNN and LSTM. The results show that (i) Mask R-CNN can accurately extract the spectral of mature leaves (precision=97.8 %) and shoots (precision=91.5 %), (ii) The spectral feature bands of shoot (number=212) and root (number=105) biomass screened by UVE were more abundant than CARS and SPA, (iii) The UVE+CNN-GRU model (Rp2=0.90, RMSEP = 0.12, RPD=2.43) based on shoots spectrum provided the optimal estimation of shoot biomass. The SPA+LSTM model (Rp2 = 0.65, RMSEP = 0.05, RPD=1.67) based on the mature leaves spectrum provided the optimal estimation of root biomass. These findings demonstrate that hyperspectral imaging technology combined with deep learning algorithms can accurately monitor the growth of cutting seedlings quickly without causing damage. This not only provides a new data sources along with technical means for efficient screening excellent tea varieties, but also improves agricultural production efficiency and resource utilization.
Read full abstract