Statement of problem: The vast majority of studies address the comparison of visual and instrumental tooth color determination. Although, the lighting conditions of the environment may affect the clinical shade matching performance. This study evaluated if and how different light sources can influence the spectrophotometric determination of natural tooth color. Purpose: The purpose of this study was to evaluate the precision of the spectrophotometer in tooth shade determination under the influence of different illuminants. Material and method: Three examiners, determined the shade of an upper central incisor with Vitapan 3D-Master (VITA Zahnfabrik, Bad Säckingen, Germany) and with the spectrophotometer (Vita Easyshade V, Vita Zahnfabrik, Bad Säckingen, Germany). A special fabricated device with eight light sources, designed to offer reproductible lighting conditions, was used to determine the efficiency of instrumental color determination under different illuminants. Results: Natural light (300 lux) and dental unit light at full intensity (32500 lux) produced the less ∆Eab modification. Color coordinates determined in the presence of halogen light (1800 lux), neon ambient light (3000 lux), LED with cold light (21200 lux), halogen light with neon ambient light (23400 lux), both warm and cold LED simultaneously (36000 lux) and all eight light sources of the device (44000 lux), suffered a slight modification of ∆E ab, which does not exceed the acceptability and the perceptibility threshold. The illuminant that caused the biggest modifications in color coordinates was LED warm light (1940 lux) with a ∆Eab 5.804 ± 0.271, that exceeds the acceptability and perceptability threshold. Conclusions: LED warm light influences the instrumental tooth color determination and when used, can lead to errors in communication of the color to the dental laboratory, which can cause esthetic failure of a prosthetic restoration. The best illuminant for color determination is natural light, or illuminants that simulate lighting in daily living conditions. Clinical implications: Based on the spectrophotometric readings, it was demonstrated that tooth color shifts with the change in ambient lighting conditions. Therefore, color determination should be performed with natural daylight illumination.
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