The objective of this study was to assess the effect of AgpP-P phytase supplementation on productivity, nutrient availability, zoo-sanitary assessment of meat and serum biochemical parameters of Hubbard broilers. A total of 180 one-day-old Hubbard broiler chickens were randomly divided into 2 groups (control and experimental) with 3 replicates and 30 broilers per replicate. In the experimental group, the diet was supplemented with phytase preparation at 1000 FTU/kg. Results showed that phytase supplementation increased body weight and absolute weight gain in Hubbard broilers, while reducing the total amount of feed consumed by birds (P < 0.05). Phytase improved the availability and absorption of organic nutrients, calcium and phosphorus. However, no differences were observed for N absorption (P<0.05). Phytase supplementation increased Ca and P contents, total protein level and alanine aminotransferase activity, and reduced urea level, aspartate aminotransferase and alkaline phosphatase activities in serum (P < 0.05). The meat of phytase-fed broilers meets Russian State Standards for fresh, good-quality meat based on organoleptic and physico-chemical characteristics. In conclusion, it was established that the addition of phytase at 1000 FTU/kg to the diet for broilers enhances the digestibility of the organic and mineral components of the feed ration, increases body weight in birds and reduces the amount of feed consumed.