AbstractLipid biomarkers archived in marine sediments include widely applied proxies to reconstruct sea surface temperature (SST). Two prominent groups of SST sensitive biomarkers are long‐chain alkenones from haptophyte algae and glycerol dibiphytanyl glycerol tetraethers (GDGTs) from planktonic Thaumarchaeota. The corresponding proxies, and TEX86, respectively, are strongly correlated with mean annual SST. However, culture experiments suggest that other factors such as nutrients could also influence the proxy signals. We created monthly resolved records of molecular SST proxies via mass spectrometry imaging at 200‐μm resolution in varved sediments from Santa Barbara Basin deposited between 1984 and 2009. Direct comparison to coeval water column data determined at seasonal resolution allows new mechanistic insights into environmental parameters influencing proxy signal formation. The responds sensitively to SST variations, while also being influenced by seasonal variations in nutrient concentrations. Surface water nitrate concentrations above or below ~2 μmol/L are associated with values that overestimate or underestimate actual SST, respectively. Distributions of the two major GDGTs, expressed as the Crenarchaeol‐Caldarchaeol‐Tetraether index (CCaT), are highly similar to those of the minor cycloalkylated compounds GDGT‐1 to GDGT‐3, presented as the corresponding ring index. The CCaT is correlated with subsurface chemical properties, which are controlled by interannual variations in upwelling intensity linked to dynamics of the California Current System. This relationship is attributed to the impact of nutrients on thaumarchaeal growth rates and GDGT cyclization. The coupling of CCaT to SST was weak but enhanced for temperatures averaged across the upper 100 m of the water column.
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