Acoustic Response Research Articles

Replayed reef sounds induce settlement of Favia fragum coral larvae in aquaria and field environmentsa).

Acoustic cues of healthy reefs are known to support critical settlement behaviors for one reef-building coral, but acoustic responses have not been demonstrated in additional species. Settlement of Favia fragum larvae in response to replayed coral reef soundscapes were observed by exposing larvae in aquaria and reef settings to playback sound treatments for 24-72 h. Settlement increased under 24 h sound treatments in both experiments. The results add to growing knowledge that acoustically mediated settlement may be widespread among stony corals with species-specific attributes, suggesting sound could be one tool employed to rehabilitate and build resilience within imperiled reef communities.

Global characterization of modelled micronekton in biophysically defined provinces

Micronekton are the mid-trophic level of the ecosystem and contribute to active carbon export to the deep ocean through diel vertical migrations. Better characterization of micronekton functional groups depending on relationships to environmental variables is useful for the management of marine resources, the conservation of biodiversity and a better understanding of climate change impacts. For this purpose, regionalization of global ocean into homogeneous provinces is an approach that is generating increasing interest. However, published regionalizations efforts (i) derived from environmental forcings, that do not specifically focus on micronekton and (ii) derived from acoustic backscatter, which do not allow direct estimates of micronekton biomass. Here, we propose to fill the gap between biophysical regionalizations and micronekton biomass. We notably defined biophysical biomes using global environmental variables known to affect micronekton: temperature of the epipelagic layer, temperature stratification, and net primary production (NPP). Six biophysical biomes were defined with a clustering method. A characterization of these biophysical biomes with simulated micronekton from the SEAPODYM-LMTL model displayed biome-specific relationships between biomass and the environmental variables used in the clustering (i.e. biomasses mostly structured by NPP and temperature). Biophysical biomes also displayed specific vertical structures suggested by modelled micronekton functional groups ratios. Then, a validation of biophysical biomes’ boundaries was performed to identify potential vertical structure reorganization in acoustic backscattering response from adjacent biomes. The regionalization identified homogeneous areas in terms of acoustic vertical structure, which were also different between adjacent biomes. Finally, a comparison with another biomes’ definition computed from micronekton biomasses suggested that environmental variables can account for only some of the variability of the micronekton structures.

Unveil the toxicity induced on early life stages of zebrafish (Danio rerio) exposed to 3,4-methylenedioxymethamphetamine (MDMA) and its enantiomers

The increased detection of the recreational drug 3,4-methylenedioxymethamphetamine (MDMA) in aquatic ecosystems, has raised concern worldwide about its possible negative impacts on wildlife. MDMA is produced as racemate but its enantioselective effects on non-target organisms are poorly understood. Therefore, this study aimed to provide a comprehensive study of the toxicity of MDMA and its enantiomers in the early life stages of zebrafish (Danio rerio). Zebrafish embryos (≈3 h post fertilization) were exposed to different concentrations (0.02, 0.2, 2, 20, and 200 μg/L) of (R,S)-MDMA and both pure enantiomers.Both enantiomers induced effects on embryonic development, DNA integrity, and behaviour and enantioselective effects were noted. (S)-MDMA exhibits higher toxic effects on embryonic development level with increased mortality and severity of teratogenic effects, and behavioural abnormalities in acoustic startle-habituation response. (R)-MDMA affected general activity and avoidance behaviour, showing greater inhibitory effects on behavioural activity. Additionally, (R,S)-MDMA induced higher genotoxic effects than the two isolated enantiomers. These results are of concern at populational levels since effects on mortality, development, and behaviour were observed even at environmentally relevant concentrations, which can cause a reduction of larval viability and in the number of individuals in each generation, and an increase in the risk of predation of the organisms. Yet, the bioaccumulation studies showed that MDMA is not accumulated in zebrafish.Therefore, this work demonstrated for the first time the occurrence of MDMA enantiotoxicity in the early life stages of zebrafish, which should be considered in further environmental risk assessments involving fish species or other non-target aquatic organisms.

Measurements of temporal variability of acoustic scattering from the seafloor in shallow-water sandy sites.

In the ocean, the performance of active sonar systems depends on the acoustic properties of the seafloor. Daily to monthly variations in near-bottom hydrodynamics and benthic biological activity may affect seafloor properties which then influence the acoustic response of the seafloor. The dependence of seafloor scatter on evolving environmental parameters was investigated using high-frequency active acoustic systems. Seafloor scattering measurements were analyzed in a series of experiments (two weeks to five months in duration) from downward-looking sonars oriented at 20° grazing angle with respect to the seafloor. Data were obtained in two shallow water locations near Portsmouth, New Hampshire, USA: a wave-dominated site and a site dominated by tidal currents. The bottom type for both sites was gravelly sand. The experimental set-up consisted of a tripod placed on the seafloor equipped with three transducers operating at 38, 70, and 200 kHz, a wave-sensing CTD, and underwater cameras. Scattering strength time series were obtained taking into account the local seafloor slope. Results show that there is variability in scattering strength (both in mean levels and distributions). Large variations often coincided with storm events, suggesting that this variability may be driven by changes in bottom roughness caused by storm-related hydrodynamics.

Investigation on Vibro-acoustic Characteristics of High-speed Railway Steel-concrete Composite Bridge Based on the Combined FE-BE-SEA Method

This paper presents a combined finite element-boundary element-statistical energy analysis (FE-BE-SEA) method to predict the vibro-acoustic characteristics of high-speed railway steel-concrete composite (SCC) bridge. The train-track coupling model is firstly introduced to accurately determine the forces transmitted to bridge, which are then imported into the established FE-BE model and SEA model. Among them, the former is adopted to calculate the vibration response in full frequency band and acoustic response at 20∼200 Hz, while for the noise part at 200∼2000 Hz, the latter is utilized for calculation. The on-site testing is carried out simultaneously to collect the measured results, which are compared with the predicted ones, validating the reliability and accuracy of the proposed method. Finally, a thorough discussion is conducted on the acoustic contribution and parametric analysis. The results indicate that the dominant frequency bands of deck and web radiated noise are below and above 200 Hz, respectively, ranking first and second in terms of overall acoustic contribution, followed by the diaphragm and flange. Reducing train speed, installing elastic fasteners, as well as increasing web thickness can significantly mitigate the vibro-acoustic responses from SCC bridge, but the effective frequency ranges for vibration and noise reduction are slightly different.

An Electronic “Tongue” Based on Multimode Multidirectional Acoustic Plate Wave Propagation

This paper theoretically and experimentally demonstrates the possibility of detecting the five basic tastes (salt, sweet, sour, umami, and bitter) using a variety of higher-order acoustic waves propagating in piezoelectric plates. Aqueous solutions of sodium chloride (NaCl), glucose (C6H12O6), citric acid (C6H8O7), monosodium glutamate (C5H8NO4Na), and sagebrush were used as chemicals for the simulation of each taste. These liquids differed from each other in terms of their physical properties such as density, viscosity, electrical conductivity, and permittivity. As a total acoustic response to the simultaneous action of all liquid parameters on all acoustic modes in a given frequency range, a change in the propagation losses (ΔS12) of the specified wave compared with distilled water was used. Based on experimental measurements, the corresponding orientation histograms of the ΔS12 were plotted for different types of acoustic waves. It was found that these histograms for different substances are individual and differ in shape, area, and position of their extremes. Theoretically, it has been shown that the influence of different liquids on different acoustic modes is due to both the electrical and mechanical properties of the liquids themselves and the mechanical polarization of the corresponding modes. Despite the fact that the mechanical properties of the used liquids are close to each other, the attenuation of different modes in their presence is not only due to the difference in their electrical parameters. The proposed approach to creating a multi-parametric multimode acoustic electronic tongue and obtaining a set of histograms for typical liquids will allow for the development of devices for the operational analysis of food, medicines, gasoline, aircraft fuel, and other liquid substances without the need for detailed chemical analysis.

Cadherin 16 promotes sensory gating via the endocrine corpuscles of Stannius.

Sensory thresholds enable animals to regulate their behavioral responses to environmental threats. Despite the importance of sensory thresholds for animal behavior and human health, we do not yet have a full appreciation of the underlying molecular-genetic and circuit mechanisms. The larval zebrafish acoustic startle response provides a powerful system to identify molecular mechanisms underlying establishment of sensory thresholds and plasticity of thresholds through mechanisms like habituation. Using this system, we identify Cadherin 16 as a previously undescribed regulator of sensory gating. We demonstrate that Cadherin 16 regulates sensory thresholds via an endocrine organ, the corpuscle of Stannius (CS), which is essential in zebrafish for regulating Ca 2+ homeostasis. We further show that Cadherin 16 regulates whole-body calcium and ultimately behavior through the hormone Stanniocalcin 1L, and the IGF-regulatory metalloprotease, Papp-aa. Finally, we demonstrate the importance of the CS through ablation experiments that reveal its role in promoting normal acoustic sensory gating. Together, our results uncover a previously undescribed brain non-autonomous pathway for the regulation of behavior and establish Ca 2+ homeostasis as a critical process underlying sensory gating in vivo .

A semi-analytical methodology for predicting the vibroacoustic response of functionally graded nanoplates under thermal loads

In this investigation, the analysis of the nonlinear vibroacoustic and sound transmission loss behaviors of nanoplates made of functionally graded materials considering the nonlocal strain gradient theory is presented. It is presumed that the properties of the functionally graded nanoplate are in the form of the simple power law scheme and continuous along the thickness, under nonlinear temperature rise and incident oblique acoustic wave as well as the first-order shear deformation theory. For this purpose, applying Hamilton’s principle, the nonlinear partial differential equations of motion are derived by the displacement field function approach and by considering the von Kármán nonlinear strain-displacement relations. To solve the equations, using the Galerkin method, the nonlinear partial differential equations of motion lead to the Duffing equation. Then, applying the homotopy analysis method, the equation of the transverse movement of the nanoplate is solved semi-analytically to obtain the nonlinear frequencies. Lastly, the nonlinear vibration and acoustic response of functionally graded nanoplate are investigated by considering the variation of the significant factors such as material gradient indices, nonlocal parameters, length scale parameters, aspect ratio, dimensionless amplitude, nonlinear temperature changes, and sound characteristics of the functionally graded nanoplate.

A Multimodal, In Vivo Approach for Assessing Structurally and Phenotypically Related Neuroactive Molecules.

A recently reported behavioral screen in larval zebrafish for phenocopiers of known anesthetics and associated drugs yielded an isoflavone. Related isoflavones have also been reported as GABAA potentiators. From this, we synthesized a small library of isoflavones and incorporated an in vivo phenotypic approach to perform structure-behavior relationship studies of the screening hit and related analogs via behavioral profiling, patch-clamp experiments, and whole brain imaging. This revealed that analogs effect a range of behavioral responses, including sedation with and without enhancing the acoustic startle response. Interestingly, a subset of compounds effect sedation and enhancement of motor responses to both acoustic and light stimuli. Patch clamp recordings of cells with a human GABAA receptor confirmed that behavior-modulating isoflavones modify the GABA signaling. To better understand these molecules' nuanced effects on behavior, we performed whole brain imaging to reveal that analogs differentially effect neuronal activity. These studies demonstrate a multimodal approach to assessing activities of neuroactives.

Acoustic discoveries of the Carinthian Gate theatre (Kärntnertortheater) of Vienna: on the trace of a lost artistical building heritage

Throughout history, many cultural heritage buildings of important value have been lost due to natural disasters as well as human causes (e.g. fire, war destruction, change of use etc.). The needs to build a larger theatre, as occurred in Vienna, addressed the Carinthian Gate theatre to have different room functions, while currently is considered the house of Sacher cake at international level. This paper deals with the discovery of the acoustic response of one of the most important theatres of Vienna. The realization of a 3D model that reproduces the architectural features allows the authors to perform acoustic simulations that reveal the sound environment that would be perceived by the audience during artistic performances at the end of eighteenth century in Vienna. In addition, the simulated results of the main acoustic parameters have been compared with the values of other opera theatres provided with similar dimensions and architectural features, as already studied by the authors. The results indicate an overall acoustic response, as typical of horseshoe shape plan layout theatres with the values of clarity found very close or within the optimal range, the sound strength ranging between 4 and 11 dB, and the reverberation time close to 1.5 s, in line with the criteria of opera theatre based on the room volume of the main hall and finished materials applied to the surfaces of the 3D model.

Insertable, dual-density dielectric barrier for acoustic pressure level reduction in a high-performance human head-only MRI system

We report use of a dual-density dielectric barrier surrounding a detachable high-pass radiofrequency (RF) birdcage coil to achieve an order-of-magnitude reduction of acoustic noise in a high-performance head gradient system. The barrier consisted of a 4.5 mm-thick mass-loaded vinyl and a 6 mm-thick polyurethane foam. It was inserted into the radial gap between the birdcage coil and the RF shield in a prototype head-only gradient system at 3 T. More than 9 dBA reduction of sound pressure level was achieved on the average with representative, high acoustic-noise imaging sequences. Increased acoustic damping was apparent from acoustic impulse response functions. High dielectric constant of the mass-loaded vinyl effectively added distributed capacitance to the birdcage coil, lowering the resonance frequency, but not seriously degrading the RF transmission performance. The barrier occupied the radial space normally used for air cooling of the RF coil and the RF shield. The resulting omission of air cooling was found to be acceptable with efficient gradient thermal management and use of a high-resistivity RF shield for eddy current reduction. The proposed method can improve patient experience while preserving image quality in a high-power head-only gradient system.

Nonlinear dynamics of a bubble cluster in liquid cavity wrapped by elastic medium

Abstract According to the modified nucleation theory, gas nuclei can generate and grow into cavitation bubbles when’s the liquid pressure exceeds a threshold in a cavity. Based on the modified nucleation theory, the growth process of the gas nuclei in a liquid cavity can be simplified to two “events”, and the full confinement effects of the surrounding elastic medium of the cavity was considered by including the bulk modulus in the equation of state. The dynamical equations were derived to model the nonlinear oscillation of the multi-bubble system in the cavity. The effects of acoustic parameters, the initial radius and the total number of bubbles on bubble dynamics were investigated numerically. It is found that bubble behaviors are sensitive to the acoustic field. In the low frequency region (f <1MHz), bubble oscillation exhibits a modulated waveform, and fundamental, subharmonic and harmonic modes can be found in the acoustic response curve. The oscillations of confined bubbles diminish with increasing frequency. In the high frequency region (f >1MHz), the confinement state affects the acoustic response of the gas nuclei, and the growth rate differ by a factor of 5 difference in the presence of shelled or unbounded elastic confinement of the cavity. Therefore, the suppression or enhancement of cavitation growth and collapse in confined cavity can be modulated by a variety of factors, such as the total number of nuclei, cavity size, acoustic frequency and amplitude, negative pressure, and elastic medium, which have a synergistic influence on the confined cavitation process.

A Unique Time-Reversal Algorithm-Enabled Flexible Ultrasound Transducer with a Controllable Acoustic Field.

Flexible ultrasonic devices represent a feasible technology for providing timely signal detection and even a non-invasive disease treatment for the human brain. However, the deformation of the devices is always accompanied by a change in the acoustic field, making it hard for accurate focusing. Herein, we report a stable and flexible transducer. This device can generate a high-intensity acoustic signal with a controllable acoustic field even when the device is bent. The key is to use a low-impedance piezoelectric material and an island-bridge device structure, as well as to design a unique time-reversal algorithm to correct the deviation of signals after transcranial propagation. To provide an in-depth study of the acoustic field of flexible devices, we also analyze the effects of mechanical deformation and structural parameters on the corresponding acoustic response.

Trapped acoustic waves and raindrops: High-order accurate integral equation method for localized excitation of a periodic staircase

We present a high-order boundary integral equation (BIE) method for the frequency-domain acoustic scattering of a point source by a singly-periodic, infinite, corrugated boundary. We apply it to the accurate numerical study of acoustic radiation in the neighborhood of a sound-hard two-dimensional staircase modeled after the El Castillo pyramid. Such staircases support trapped waves which travel along the surface and decay exponentially away from it. We use the array scanning method (Floquet–Bloch transform) to recover the scattered field as an integral over the family of quasiperiodic solutions parameterized by on-surface wavenumber. Each such BIE solution requires the quasiperiodic Green's function, which we evaluate using an efficient integral representation of lattice sum coefficients. We avoid the singularities and branch cuts present in the array scanning integral by complex contour deformation. For each frequency, this enables a solution accurate to around 10 digits in a few seconds. We propose a residue method to extract the limiting powers carried by trapped modes far from the source. Finally, by computing the trapped mode dispersion relation, we use a simple ray model to explain an acoustic chirp-like time-domain response that is referred to in the literature as the “raindrop effect.”

Free-Form Deformation as a non-invasive, discrete unfitted domain method: Application to the time-harmonic acoustic response of a saxophone

The Finite Element method, widely used for solving Partial Differential Equations, may result in suboptimal computational costs when computing smooth fields within complex geometries. In such situations, IsoGeometric Analysis often offers improved per degree-of-freedom accuracy but building analysis-suitable representation of complex shapes is generally not obvious. This paper introduces a non-invasive, spline-based fictitious domain method using Free-Form Deformation to efficiently solve the Helmholtz equation in complex domains, such as in musical instruments. By immersing a fine FE mesh into a simple B-spline box, the approximation subspace size is significantly reduced without compromising accuracy. Accompanied by specific conditioning treatment, the method not only proves to be efficient, but also robust and easy to implement in existing FE software. Applied to an alto saxophone, the method reduces the number of degrees of freedom by over two orders of magnitude and the computation time by more than one compared to standard FE methods with comparable accuracy when compared to experimental tests.

Functionalized monodisperse microbubble production: microfluidic method for fast, controlled, and automated removal of excess coating material

Functionalized monodisperse microbubbles have the potential to boost the sensitivity and efficacy of molecular ultrasound imaging and targeted drug delivery using bubbles and ultrasound. Monodisperse bubbles can be produced in a microfluidic flow focusing device. However, their functionalization and sequential use require removal of the excess lipids from the bubble suspension to minimize the use of expensive ligands and to avoid competitive binding and blocking of the receptor molecules. To date, excess lipid removal is performed by centrifugation, which is labor intensive and challenging to automate. More importantly, as we show, the increased hydrostatic pressure during centrifugation can reduce bubble monodispersity. Here, we introduce a novel automated microfluidic ’washing’ method. First, bubbles are injected in a microfluidic chamber 1 mm in height where they are left to float against the top wall. Second, lipid-free medium is pumped through the chamber to remove excess lipids while the bubbles remain located at the top wall. Third, the washed bubbles are resuspended and removed from the device into a collection vial. We demonstrate that the present method can (i) reduce the excess lipid concentration by 4 orders of magnitude, (ii) be fully automated, and (iii) be performed in minutes while the size distribution, functionality, and acoustic response of the bubbles remain unaffected. Thus, the presented method is a gateway to the fully automated production of functionalized monodisperse microbubbles.

Polymeric Microbubble Shell Engineering: Microporosity as a Key Factor to Enhance Ultrasound Imaging and Drug Delivery Performance.

Microbubbles (MB) are widely used as contrast agents for ultrasound (US) imaging and US-enhanced drug delivery. Polymeric MB are highly suitable for these applications because of their acoustic responsiveness, high drug loading capability, and ease of surface functionalization. While many studies have focused on using polymeric MB for diagnostic and therapeutic purposes, relatively little attention has thus far been paid to improving their inherent imaging and drug delivery features. This study here shows that manipulating the polymer chemistry of poly(butyl cyanoacrylate) (PBCA) MB via temporarily mixing the monomer with the monomer-mimetic butyl cyanoacetate (BCC) during the polymerization process improves the drug loading capacity of PBCA MB by more than twofold, and the in vitro and in vivo acoustic responses of PBCA MB by more than tenfold. Computer simulations and physisorption experiments show that BCC manipulates the growth of PBCA polymer chains and creates nanocavities in the MB shell, endowing PBCA MB with greater drug entrapment capability and stronger acoustic properties. Notably, because BCC can be readily and completely removed during MB purification, the resulting formulation does not include any residual reagent beyond the ones already present in current PBCA-based MB products, facilitating the potential translation of next-generation PBCA MB.

Development of a High-Sensitivity Acoustic Sensor Based on Grating Interferometer Combined with Glass Diaphragm.

In this study, based on the principle of grating interferometer-based acoustic sensors, design guidelines for the grating interferometric module were obtained and analyzed considering various factors in order to obtain high sensitivity, and a glass-based grating interference component and its acoustic sensor device were developed. The key parameters of the grating interference structure were extracted, and their effects on the acoustic response sensitivity were quantified for multiple mechanisms. For the development of the acoustic sensor, the grating-on-convex-platform structure and the micromachining processes of the glass-based components were designed and developed. The developed acoustic sensor samples achieved high sensitivity. In particular, the sample suitable for low-frequency application obtained a sensitivity of 0.776 V/Pa @ 1 kHz, and the spectrum of its sensitivity was flat from 50 Hz to 8 Hz with a deviation less than 1.5 dB and a sensitivity of 0.408 V/Pa @ 20 Hz.

Characterization and differences of acoustic signals response of semi-circular red sandstone under combined monotonous and cyclic loadings

After underground coal mining, rocks are often subjected to tensile damage by the interaction of dynamic and static loadings. The process of rock fracture development under dynamic and static loadings will be released in the form of acoustic energy to form an acoustic signal. In addition, the acoustic signals in dynamic loading differ from that in static loading. Therefore, this study conducted three-point bending experiments with continuous dynamic loading and dynamic–static coupling loading on semi-circular red sandstone specimens. The acoustic signals during red sandstone specimens’ tensile damage were monitored in real-time. The results show that red sandstone’s tensile strength and deformation are enhanced under dynamic–static coupling loading. The red sandstone has a more effective acoustic emission hit rate, energy rate, and r during tensile damage under continuous dynamic loading. In dynamic loading, macroscopic fractures are developed in red sandstone, which has few acoustic emission events but releases strong acoustic signals. In static loading, the pores inside the red sandstone are compacted, the rock particles are rearranged, and the tiny fractures are closed, and its acoustic emission events are many but low in energy. In addition, the rock particles in the front area of the static loading fracture are tightly cemented, which increases the difficulty of separating the rock particles in the front area of the fracture under dynamic loading. Then weakening the red sandstone fracture development process and suppressing its acoustic signals. The research results provide more insight into the differences in tensile damage processes in red sandstone under the interaction of dynamic and static loadings.

Ultra-thin DLC diaphragm-based optical fiber sensor achieving a highly sensitive and broadband acoustic response.

In the design of an extrinsic Fabry-Perot interferometer (EFPI) acoustic sensor, broadband response and high-sensitivity sensing are usually conflicting and need to be carefully balanced. Here, we present a novel, to the best of our knowledge, optical fiber acoustic sensor based on an ultra-thin diamond-like carbon (DLC) film, fabricated using the plasma-enhanced chemical vapor deposition method, and transferred by a surface-energy-assisted method. The sensor exhibits a broadband response ranging from 200 Hz to 100 kHz, maintains an average sensitivity of 457.3 mV/Pa within the range of 6 to 30 kHz, and can detect weak acoustic signals down to 3.23 µPa/Hz1/2@16.19 kHz. The combination of an ultra-thin DLC film with a relatively high Young's modulus and internal stresses results in a trade-off between high sensitivity and a broadband response. This performance demonstrates that our sensor is among the most advanced in the EFPI acoustic sensor family, with significant potential for applications such as photoacoustic spectroscopy, defect diagnosis, and bio-imaging.