Acetylcholine Provocation Test Research Articles

Omitting 50 μg of intracoronary acetylcholine injection in the left coronary artery during spasm provocation test

BackgroundAlthough guidelines recommend intracoronary administration of acetylcholine (ACh) with incremental doses of 20, 50, and 100 μg into the left coronary artery (LCA) during spasm provocation test for diagnosing vasospastic angina, 50 μg of ACh rarely induced significant coronary vasospasm when no vasoconstriction was observed with 20 μg of ACh in a previous report. The aim of this study was to evaluate the safety and feasibility of omitting 50 μg according to the vasoreactivity by 20 μg of ACh in the LCA. MethodsA total of 556 patients undergoing ACh provocation test with 20 μg followed by 50 and/or 100 μg were retrospectively included. Injection of 50 μg of ACh was primarily omitted when vasoconstriction <25 % was observed with 20 μg, which was left to operator's discretion. Adverse events were defined as a composite of ventricular fibrillation, sustained ventricular tachycardia, and cardiogenic shock during ACh test in the LCA. ResultsPositive ACh test in the LCA was observed in 245 (44.1 %) patients. Overall, patients with LCA constriction <25 % by 20 μg of ACh had a lower rate of positive ACh test than their counterpart (24.0 % vs. 88.4 %, p < 0.001). In patients with LCA constriction ≥25 % by 20 μg, the incidence of adverse events was significantly higher than in those with LCA constriction <25 % during the provocation test at doses of 50 and 100 μg (2.3 % vs. 0 %, p = 0.009). ConclusionsOmitting 50 μg of ACh in the LCA may be safe and feasible when little vasoconstriction was observed with preceding injection of 20 μg of ACh during spasm provocation test for diagnosing vasospastic angina. However, we believe that 50 μg of ACh should not be omitted when 20 μg of ACh induced LCA constriction ≥25 %.

Differences in Clinical and Angiographic Characteristics Following Intracoronary Ergnovine and Acetylcholine Provocation Test in Hypertension Patients

Differences in Clinical and Angiographic Characteristics Following Intracoronary Ergnovine and Acetylcholine Provocation Test in Hypertension Patients

Differences in Clinical and Angiographic Parameters Following Intracoronary Ergonovine and Acetylcholine Provocation Test in Elderly Patients

Differences in Clinical and Angiographic Parameters Following Intracoronary Ergonovine and Acetylcholine Provocation Test in Elderly Patients

Utility of Temporary Pacemaker During Acetylcholine Provocation Testing

Utility of Temporary Pacemaker During Acetylcholine Provocation Testing

Impact of preceding acetylcholine provocation testing on following coronary physiological assessment during an interventional diagnostic procedure

BackgroundIntracoronary acetylcholine (ACh) provocation test and coronary physiological assessment are useful interventional diagnostic procedures for evaluating ischemia with no obstructive coronary arteries (INOCA). However, the appropriate sequential order of the diagnostic procedures has been a matter of debate. We investigated the impact of preceding ACh provocation on following coronary physiological assessment. MethodsPatients suspected of INOCA underwent invasive coronary physiological assessment using thermodilution method and were divided into two groups according to the implementation of ACh provocation test. The ACh group was further divided into the positive and negative ACh groups. In the ACh group, intracoronary ACh provocation was performed before the invasive coronary physiological assessment. The main interest of this study was to compare coronary physiological indices among the no ACh, negative ACh, and positive ACh groups. ResultsOf 120 patients, the no ACh, and negative and positive ACh groups included 46 (38.3 %), 36 (30.0 %), and 38 (31.7 %), respectively. Fractional flow reserve was lower in the no ACh group than in the ACh group. Resting mean transit time was significantly longer in the positive ACh group, followed by the no ACh and negative ACh groups (1.22 ± 0.55 vs. 1.00 ± 0.46 vs. 0.74 ± 0.36 s, p < 0.001). Index of microcirculatory resistance and coronary flow reserve did not differ significantly among the three groups. ConclusionsPreceding ACh provocation influenced following physiological assessment, particularly when ACh test was positive. Further studies are warranted to determine which interventional diagnostic procedure, ACh provocation or physiological assessment, should be preceded in the invasive evaluation of INOCA.

Post-spastic flow recovery time to document vasospasm induced ischemia during acetylcholine provocation testing

BackgroundIntracoronary acetylcholine (ACh) provocation is an established method for diagnosing epicardial and microvascular vasospasm in contemporary clinical practice. We hypothesize that ACh-induced vasospasm is followed by post-spastic reactive hyperemia (PSRH), which is measured as an increased flow-recovery time. ObjectivesTo assess flow-recovery time, indicative of ischemia, among the diagnostic endotypes that follow ACh provocation testing. MethodsPatients with angina and non-obstructive coronary artery disease on angiography who underwent ACh provocation testing were included in this analysis. Doppler flow was continuously measured during the procedure and used to determine the flow-recovery time, which was calculated as time between cessation of ACh infusion and the point of flow recovery. ResultsConventional provocation testing according to the COVADIS criteria diagnosed vasospasm in 63%(77/123), an equivocal result in 22%(27/123) and a negative result in 15%(19/123) of patients. In reaction to the highest-dose of ACh, flow-recovery time was significantly extended and similar in the epicardial, microvascular and equivocal test results compared to the negative result (all p < 0.001) indicative of PSRH. ConclusionFlow-recovery time in patients with an equivocal result is similar to patients with vasospasm, which indicates the occurrence of myocardial ischemia and therefore, these patients may benefit from medical treatment.

The impact of cardiac SPECT in risk stratification for women with suspected coronary artery disease in a very high cardiovascular risk country

Abstract Funding Acknowledgements Type of funding sources: None. Background Risk stratification has become increasingly important in the management of women with coronary artery disease (CAD). Population based cross-sectional study of cardiovascular risk (CVR) factors in Latvia revealed that women in age 25 -74 are in a very high CVR group: more than 60% of women have dyslipidemia, 30% have arterial hypertension (AH) and are overweight. Purpose To evaluate CVR factor profile in women with suspected CAD and performed cardiac single photon emission computed tomography (SPECT) and to prove the need of perfusion imaging for women in a very high cardiovascular risk population. Methods Cardiac SPECT for women with suspected CAD and positive or non-informative exercise test was performed in a single center from 2018 till 2022. The patients were analyzed by risk factors and perfusion test result, divided into 2 groups: normal myocardial perfusion or true myocardial perfusion defect. Results 466 patients without known CAD were analyzed. The mean age of cohort patients was 59.3±10.9 years. 28 (6.0%) patients had no CVR factors, 90 (19.3%) women had only 1, but the rest of them (348, 74.7%) had 2 or more CVR factors. Dyslipidemia was present in 358 (76.8%), AH in 282 (60.5%) and type 2 Diabetes mellitus (T2DM) in 27 (5.8%) patients. More than half of patients (n=303, 65.0%) were overweight (BMI&amp;gt;25.0 kg/m2). 65 (13.9%) women were current or ex-smokers. True myocardial perfusion defect was detected in 96 patients (20.6%). There was no statistically significant difference between CVR risk factor count and cardiac SPECT result (p=0.229), although there were patients with significant perfusion defect without any risk factor present. However, significant correlation between risk factors and detection of obstructive coronary artery disease (CAD) after positive cardiac SPECT was found (p&amp;lt;0.001). Totally in study was detected 71 patients with suspected ischemia with non-obstructive CAD (INOCA), but further investigation with intracoronary physiology measurements – coronary flow reserve (CFR), index of microvascular resistance (IMR) and acetylcholine provocation test is required to define INOCA endotype. Conclusions The cardiac SPECT is an appropriate diagnostic method for women patients with suspected CAD. Even in patients with no CVR factors, but with positive or non-informative exercise test, the CAD is present, therefore they should be referred to cardiac SPECT in a very high CVR country. In patients with significant perfusion defect and absence of obstructive CAD, the performance of intracoronary physiology measurements is required.

Resuscitated sudden cardiac arrest related to intraplaque haemorrhage and coronary artery spasm.

A 72-year-old man with a history of chronic kidney disease, dyslipidaemia, and hypertension felt chest tightness after walking uphill and sat down to rest. Shortly thereafter, he suddenly collapsed, and cardiopulmonary resuscitation was started by a nearby family member. The initial electrocardiogram waveform was ventricular fibrillation, which was restored to sinus rhythm with an automated external defibrillator. He was transported to our hospital. No significant electrocardiographic changes, but positive serum troponin was observed on arrival. Emergency coronary angiography was performed, revealing a moderate stenosis in the proximal left anterior descending coronary artery (Panel A) which was functionally significant (Panel B). Optical coherence tomography showed a large eccentric plaque with a confined low-intensity plaque with strong signal attenuation (Panel C) and some areas of low intensity and low attenuation (Panel D), highly suggestive of intraplaque haemorrhage (IPH) (see Supplementary data online, Video S1).1 A stent implantation was subsequently performed (Panel E). On the ninth day of hospitalization, an acetylcholine provocation test was performed for the right coronary artery and severe diffuse coronary spasm was induced (Panels F and G) accompanied by chest tightness and ST elevation on electrocardiogram, leading to a diagnosis of coronary spastic angina.

CRT-100.63 Differences in Clinical and Angiographic Characteristics Following Intracoronary Ergnovine and Acetylcholine Provocation Test in Hypertension Patients

CRT-100.63 Differences in Clinical and Angiographic Characteristics Following Intracoronary Ergnovine and Acetylcholine Provocation Test in Hypertension Patients

CRT-100.45 Differences in Clinical and Angiographic Parameters Following Intracoronary Ergonovine and Acetylcholine Provocation Test in Elderly Patients

CRT-100.45 Differences in Clinical and Angiographic Parameters Following Intracoronary Ergonovine and Acetylcholine Provocation Test in Elderly Patients

Angina pectoris und die Bedeutung der koronaren Mikrozirkulation in der Praxis

Angina Pectoris and the Importance of Coronary Microcirculation in Practice Abstract. Microvascular angina is a common manifestation of coronary microvascular dysfunction, particulary prevalent in post-menopausal women above the age of 50 and associated with impaired quality of life and poor clinical outcomes. However, microvascular angina remains largely undetected given the underuse of diagnostic tools for the assessment of coronary microvascular function. As a consequence, many of these patients suffering from coronary microvascular dysfunction fail to receive the appropriate medical treatment and remain in the long term symptomatic. Invasive coronary catheterization with measurement of coronary flow reserve and intracoronary acetylcholine provocation testing allows for the assessment of coronary microvascular dysfunction, and a therapy targeting specific physiological pathways can be implemented. A targeted therapy includes lifestyle modifications, secondary prevention measures, and anti-anginal medication. Ongoing clinical research in the field is expected to deliver novel diagnostic and therapeutic concepts for an improved management of patients with coronary microvascular disease.

Myocardial bridge evaluation towards personalized medicine: study design and preliminary results of the RIALTO registry.

Myocardial bridge (MB) is the most frequent inborn coronary artery variant in which a portion of the myocardium overlies an epicardial coronary artery segment. Although MB has long been considered a benign entity, a growing body of evidence has suggested its association with angina and adverse cardiac events. However, to date, no data on long-term prognosis are available, nor on therapies improving cardiovascular outcomes. We are currently conducting an ambispective, observational, multicentre, study in which we enrol patients with a clinical indication to undergo coronary angiography (CA) and evidence of MB, aiming to describe the incidence of symptoms and cardiovascular events at baseline and at long-term follow-up (FUP). The role of invasive full-physiology assessment in modifying the discharge therapy and eventually the perceived quality of life and the incidence of major cardiovascular events will be analysed. Basal clinical-instrumental data of eligible and consenting patients have been acquired after CA; FUP was performed 6, 12, and 24 months after the angiographic diagnosis of MB. The primary endpoint of the study is the incidence of major adverse cardiovascular events (MACE), defined as the composite of cardiac death, myocardial infarction, cardiac hospitalization, and target vessel revascularization; the secondary endpoints are the rate of patients with Seattle Angina Questionnaire (SAQ) summary score <70 and the incidence of MACE in patients undergoing invasive intracoronary assessment. Among patients undergone FUP visits, we recorded 31 MACE at 6 months (11.6%), 16 MACE at 12 months (6.5%), and 26 MACE at 24 months (13.5%). The rate of patients with SAQ <70 is 18.8% at 6 months, 20.6% at 12 months, and 21.8% at 24 months. To evaluate the prognostic role of invasive intracoronary assessment, we compared MB patients who underwent only angiographic evaluation (Angio group) to those who underwent acetylcholine (ACH) provocative test with indication to calcium-channel blockers (CCBs) at discharge (Angio + ACH + CCBs group) and those who underwent functional assessment with fractional flow reserve (FFR) with indication to beta-blockers (BBs) at discharge (Angio + FFR + BBs group). After 2 years of FUP, the rate of MACE was significantly reduced in both Angio + ACH + CCBs group (6 vs. 25%, P = 0.029) and Angio + FFR + BBs group (3 vs. 25%, P = 0.005) compared with Angio group. The preliminary results of our study showed that MB may be a cause of angina and adverse cardiac events in patients referred to CA for suspected coronary artery disease (CAD). Full-physiology assessment unmasking MB-related ischaemia mechanisms, allowed to guide the treatment, personalizing the clinical management, improving the quality of life, and cardiovascular outcomes in patients with MB.

Abstract 14339: Differences in Clinical and Angiographic Characteristics Following Intracoronary Ergnovine and Acetylcholine Provocation Test for Coronary Artery Spasm

Background: Ergnovine (Erg) and Acetylcholine (Ach) are useful agents for assessing significant coronary artery spasm (CAS). However, comparison studies of the two agents are limited. Methods: A total of 8,093 consecutive patients (pts) who underwent Erg or Ach provocation test between November 2004 and June 2021 were enrolled. Significant CAS was defined as focal or diffuse severe transient luminal narrowing (&gt;70%) with/without chest pain or ST-T change on ECG. We divided into two groups according to provocation agent; 1) the Erg group (n=639), 2) the Ach group (n = 7,454). We investigated whether there are differences in clinical and angiographic characteristics according to intracoronary Erg or Ach provocation test. Results: Baseline clinical characteristics were similar between the two groups except the Erg group were more elderly (58.5 years vs 56.6 years, p &lt; 0.001) and dyslipidemia (36.0% vs 18.6%, p &lt; 0.001) but lower rates of current smokers (16.0% vs 21.1%, p = 0.002) and alcoholics (27.1% vs 32.9%, p = 0.002). During the provocation test, the higher rates of myocardial bridge, ECG change, chest pain, atrioventricular node block, and positive CAS results but lower rates of baseline spastic change were shown in the Ach group. More pts received to lower Ach dose and higher Erg dose during the provocation test. In CAS positive patients, the Ach group had higher rates of spasm in the left anterior descending artery and mid site, but lower proximal site (table). Conclusion: Clinical and angiographic parameters were different between Erg and Ach provocation tests. Further study would be needed to validate the different parameters and different positive rates between the two agents. Table. Angiographic and Procedural Parameters during Ergonovine versus Acetylcholine Provocation Test

Epicardial atherosclerosis and coronary tortuosity in patients with acetylcholine-induced coronary spasm.

Angina pectoris in the absence of relevant epicardial stenoses is frequently caused by coronary spasm. This mechanism of angina is common yet underdiagnosed in daily clinical practice. The pathophysiology of coronary spasm is complex, multifactorial, and not completely understood. The purpose of this study was to analyze the relationship between macroscopic coronary morphologies and coronary spasm. Epicardial atherosclerosis, coronary vessel tortuosity, coronary aneurysms, and myocardial bridges were analyzed angiographically in 610 patients and a potential association with the result of an intracoronary acetylcholine (ACh) provocation test was investigated. The comparison showed that angiographic morphologic variations in the coronary arteries are related to the occurrence of coronary spasm. We observed a strong association between the presence of epicardial atherosclerosis and epicardial spasm [87 patients of 179 with epicardial spasm had epicardial atherosclerosis (49%) vs. 45 patients of 172 with microvascular spasm (26%) vs. 89 patients of 259 with negative/inconclusive ACh test (36%); P < 0.005]. Moreover, we found a higher frequency of coronary tortuosity in patients with microvascular spasm [99 patients of 172 with microvascular spasm had at least moderate coronary tortuosity (58%) vs. 76 patients of 179 with epicardial spasm (43%) vs. 126 patients of 259 with negative/inconclusive ACh test (49%); P = 0.017]. Multivariable analysis revealed epicardial atherosclerosis (<50% stenosis) on coronary angiography as a predictor for epicardial spasm (OR, 2.096; 95% CI, 1.467-2.995; P < 0.0005). Female sex (OR, 5.469; 95% CI, 3.433-8.713; P < 0.0005), and exertional angina (OR, 2.411; 95% CI, 1.597-3.639; P < 0.0005) were predictors of microvascular spasm in multivariable analysis. In angina patients with no obstructive coronary artery disease, epicardial atherosclerosis is associated with ACh-induced epicardial coronary spasm. Moreover, coronary microvascular spasm is more prevalent in female patients and those with exertional angina. Our results provide insights into the relationship between coronary morphology and coronary vasomotor function.

Post-occlusive reactive hyperaemia can objectify the occurrence of vasospasm induced ischemia during acetylcholine provocation testing

Abstract Introduction Acetylcholine (ACh) spasm provocation testing together with the diagnostic criteria published by the Coronary Vasomotor Disorders International Study Group (COVADIS) are considered the gold standard for the diagnosis of coronary vasospasm in contemporary clinical practice. In 20–29% of patients undergoing vasospasm provocation an equivocal test result occurs when these criteria are only partially met and poses a diagnostic dilemma. Post-occlusive reactive hyperaemia is a marker of acute myocardial ischemia and could serve as an added objective diagnostic criterium for the diagnosis of acetylcholine induced vasospasm. Purpose The aim of this study is therefore to evaluate the occurrence of post-occlusive reactive hyperaemia in the equivocal, positive and negative test result that follows acetylcholine vasospasm provocation. Methods 66 patients with angina and non-obstructive coronary artery disease on angiography (ANOCA) who underwent the entire ACh spasm provocation testing protocol were included in this analysis. After each ACh dose angiography was performed and when vasospasm occurred 200 μg nitro-glycerine was administered intracoronary. Definitions and diagnostic criteria for CAS were in line with those used by the COVADIS working group to diagnose epicardial and microvascular vasospasm. An equivocal test result was defined as the induction of recognisable angina without ischemic ECG changes, in reaction to ACh. Doppler flow was continuously measured during the procedure. Changes in flow due to ACh mediated vasodilation quickly return to baseline values due to its short-half life. Therefore, the occurrence of post-ischemic reactive hyperaemia was assessed by the extended time to return to baseline flow, the so called “flow recovery time”. Results We analysed the flow recovery time in 21 patients with epicardial vasospasm, in 16 with microvascular spasm, in 14 with an equivocal test result and in 15 with a negative test result. Mean age of the entire study population was 56±11 years and 86% were female. In reaction to dose 1 to 3 flow recovery time was similar between all diagnostic endotypes. In reaction to the fourth dose flow recovery time was extended in the epicardial, microvascular and equivocal test result compared to the negative test result indicative of post-ischemic reactive hyperaemia. Conclusion Post-occlusive reactive hyperaemia occurs in patients with an equivocal test result similar to patients with microvascular vasospasm and epicardial vasospasm. These findings indicate the occurrence of acetylcholine induced vasospasm in the equivocal test result and these patients may benefit from medical treatment. Funding Acknowledgement Type of funding sources: None.

Thermodilution-derived coronary absolute flow and resistance in patients with different INOCA endotypes

Abstract Background Thermodilution-derived measurement of absolute coronary flow and resistance has been recently validated by using a dedicated microcatheter and a pressure-temperature guidewire. Coronary absolute flow (AbsF) and derived absolute resistance (AbsR) can give precious information about the microcirculation in patients with ischemia and non-obstructive coronary artery disease (INOCA). The correlation with different INOCA endotypes has been so far poorly investigated. Methods 108 INOCA patients underwent a complete evaluation of underwent coronary function testing, including acetylcholine (ACH) provocation testing, adenosine testing (CFR/IMR) and functional assessment of the left anterior descending artery using thermodilution-derived measurement of AbsF and AbsR. Results Among 108 INOCA patients, 64% showed coronary microvascular dysfunction (CMD) with positive ACH reaction (ACH+) and/or abnormal CFR/IMR (CFR/IMR+), of whom 38% presenting with an isolated structural component (CFR/IMR+) and 62% with an isolated vasospastic component (ACH+). Among ACH+ patients, epicardial vasospastic angina was detected in 28.2% of the patients. AbsF was found significantly lower in ACH+ patients than in ACH− patients (0.174±0.09 vs 0.2019±0.09, p=0.03). Similarly, a trend of higher AbsR values was found in ACH+ patients as compared to ACH− (612.5±226.5 vs 524.5±171.2, p=0.07, Fig. 1A). AbsF was found significantly lower in patients with coronary microvascular dysfunction (CMD) than in patients without either vasospastic or structural component of CMD (0.172±0.06 vs 0.202±0.09, p=0.05) and AbsR showed a trend towards higher values in patients with CMD than patients without CMD. (609.3±208.8 vs 557.7±193.1, p=0.08, Fig. 1B) AbsF and AbsR did not differ in patients with and without abnormal adenosine testing only: AbsF (0.176±0.06 vs 0.197±0.09, p=0.31) and AbsR 589.8±183.0 vs 565.2±190.2, p=0.27). A modest correlation was found between AbsQ and IMR (r: −0.2: p: 0.05, Fig. 2). Conclusion Coronary vasomotor dysfunction is highly prevalent in INOCA patients and a complete coronary function evaluation, including provocative test should be performed. Continuous thermodilution is safe and easily implemented in INOCA patients and provide new insights in the assessment of INOCA endotypes with high reproducibility. The exact diagnostic and prognostic value of these measurements, and their optimal cutoff, should be assessed in future studies. Funding Acknowledgement Type of funding sources: None.

Safety and prognostic relevance of acetylcholine testing in patients with stable myocardial ischaemia or myocardial infarction and non-obstructive coronary arteries.

Intracoronary provocation testing with acetylcholine (ACh) is crucial for the diagnosis of functional coronary alterations in patients with suspected myocardial ischaemia and non-obstructive coronary arteries. Our intention was to assess the safety and predictive value for major adverse cardiovascular and cerebrovascular events (MACCE) in patients presenting with ischaemia with non-obstructive coronary arteries (INOCA) or with myocardial infarction with non-obstructive coronary arteries (MINOCA). We prospectively enrolled consecutive INOCA or MINOCA patients undergoing intracoronary ACh provocation testing. A total of 317 patients were enrolled: 174 (54.9%) with INOCA and 143 (45.1%) with MINOCA. Of these, 185 patients (58.4%) had a positive response to the ACh test. Complications during ACh provocative testing were all mild and transient and occurred in 29 (9.1%) patients, with no difference between patients with positive or negative responses to ACh testing, nor between INOCA and MINOCA patients. A history of paroxysmal atrial fibrillation, moderate/severe diastolic dysfunction and a higher QTdispersion at baseline electrocardiogram were independent predictors of complications. MACCE occurred in 30 patients (9.5%) during a median follow-up of 22 months. The incidence of MACCE was higher among patients with a positive ACh test (24 [13.0%] vs 6 [4.5%], p=0.017), and a positive ACh test was an independent predictor of MACCE. ACh provocation testing is associated with a low risk of mild and transient complications, with a similar prevalence in both INOCA and MINOCA patients. Importantly, ACh provocation testing can help to identify patients at higher risk of future clinical events, suggesting a net clinical benefit derived from its use in this clinical setting.

Prognostic impact of nitrate therapy in patients with myocardial bridge and coexisting coronary artery spasm.

The aim of this study was to investigate the prognostic impact of nitrate therapy in patients with myocardial bridge (MB) and coexisting coronary artery spasm (CAS). MB often accompanies CAS. Nitrates have been widely used as anti-ischemic drugs in CAS patients, while it is not recommended in MB patients. Thus, we investigated the long-term impact of nitrate on clinical outcomes in patients with both CAS and MB. A retrospective observational study was performed using propensity score matching (PSM) in a total of 757 consecutive MB patients with positive acetylcholine (Ach) provocation test. Patients were divided into two groups according to the regular administration of nitrates (nitrate group: n = 504, No nitrate group; n = 253). The PSM was used to adjust for selection bias and potential confounding factors, and major clinical outcomes were compared between the two groups up to 5years. Baseline characteristics were well-matched between the two groups following PSM (n = 211 for both groups). There was no significant difference in the incidence of death, myocardial infarction, and major adverse cardiovascular events (MACEs) between the two groups. However, the nitrate group showed a significantly higher rate of recurrent angina which subsequently needed re-evaluation of coronary arteries by follow-up angiography (15.7 vs. 5.7%, Log-rank p = 0.012) compared to the non-nitrate group. Long-term nitrate administration in patients with MB and coexisting CAS did not show benefit in reducing MACE, rather it was associated with a higher incidence of recurrent angina requiring follow-up angiography.

Sex Difference in Coronary Artery Spasm Tested by Intracoronary Acetylcholine Provocation Test in Patients with Nonobstructive Coronary Artery Disease.

Introduction Cardiovascular diseases manifest differently in men and women. The purpose of this study is to compare the sex difference in the characteristics of coronary artery spasm (CAS) in patients with nonobstructive cardiovascular disease (NOCVD) and the clinical outcomes in accordance with sex in CAS patients. Methods The study analysed 5,491 patients with NOCVD who underwent an acetylcholine provocation test from November 2004 to May 2014 for evaluation of chest pain. CAS was defined as greater than 70% of luminal narrowing of the artery during the acetylcholine provocation test. Results The patients were divided into men (n = 2,506) and women (n = 2,985). Mean follow-up days were 1,218 ± 577 days. To adjust for confounding factors, the propensity score matching (PSM) analysis was performed in all patients and among the CAS patients. After PSM analysis, a total of 1,201 pairs in all patients and a total of 713 pairs in CAS patients were generated. In all patients, women showed significantly less incidence of CAS compared with men (62.3% vs 50.9%, P < 0.01). Myocardial bridge (MB) and moderate stenosis were less prevalent in women, while transient ST elevation and ischemic chest pain during provocation were more frequent in women. In CAS patients, men had a higher incidence of multivessel spasm than women (35.7% vs. 29.7%, P < 0.01). Old age, dyslipidemia, and MB were independent risk factors of CAS in both men and women. In CAS patients, there was no statistical differences for various individual and composite major outcomes up to five years in either men or women. In men with CAS, old age was a risk factor of a 5-year major adverse cardiac event (MACE), and moderate stenosis was a risk factor of both 5-year MACE and 5-year recurrent angina. In women with CAS, mild stenosis was a risk factor of 5-year MACE, while myocardial bridge was a risk factor of 5-year recurrent angina. Conclusions In this study, there were sex differences in the angiographic and clinical parameters during the acetylcholine provocation test, incidence of CAS, risk factors of CAS, 5-year MACE, and recurrent angina. Old age, dyslipidemia, and MB were independent risk factors of CAS in both sexes. However, major clinical outcomes up to five years in CAS patients were not different according to sex.

TCT-296 Post-Spastic Flow Recovery Time to Document Vasospasm Induced Ischemia During Acetylcholine Provocation Testing

TCT-296 Post-Spastic Flow Recovery Time to Document Vasospasm Induced Ischemia During Acetylcholine Provocation Testing