Introduction. Biosafety standards are principles that, correctly applied, prevent occupational accidents in the clinical laboratory. Objectives. Determine the level of knowledge and application of biosafety standards and their relationship with occupational accidents in clinical laboratory workers in the Jaén health subregion, Peru, 2019. Material and methods. Analytical observational study carried out in a population of 50 workers. The level of knowledge and application of biosafety standards, as well as the history of occupational accidents, were assessed using structured and previously validated questionnaires. Results. 30.0% (15/50) and 14% (7/50) of the workers observed a high and low level of knowledge of biosafety regulations, respectively; Likewise, 68.0% (34/50) said they applied them. Regarding the history of work accidents, 36.0% (18/50) of the workers stated that they had had a work accident, of which 55.6% (10/18) were due to needle or capillary puncture. The bivariate analysis showed that personnel who do not apply biosafety standards are twice as likely to have a work accident (PR: 2.13; 95% CI: 1.03-4.41). Conclusions. A significant percentage of clinical laboratory workers in Jaén, Peru, 2019, are unaware of and do not apply biosafety standards, which is related to workplace accidents, mainly due to sharp objects.