Abundance Of Arbuscular Mycorrhizal Fungi Research Articles

Species Richness of Arbuscular Mycorrhizal Fungi in Heterogenous Saline Environments

Sabkha (inland and coastal—saline beds or saline lands) are widespread in Saudi Arabia and are distinguished by their hypersaline nature. These hypersaline habitats are commonly covered by halophytic vegetation. Moreover, Arbuscular mycorrhizal fungi (AMF) are an essential component of these habitats and exhibit a unique adaptation and contribute significantly to ecosystem variability, diversity, and function. Additionally, AMF from saline habitats are an essential component for the successful rehabilitation of salinity-affected areas. Despite their importance, little is known about the distribution and abundance of AMF along inland and coastal sabkhat of Saudi Arabia. Therefore, the main objective of this study was to investigate the abundance and diversity of AMF in the coastal and inland sabkhat of Saudi Arabia. Five soil samples, each from five randomly selected spots (considering the presence of dominant and co-dominant halophytic species), were collected from every location and were used to assess the AMF abundance and diversity. The study indicated that the highest number of AMF spores was recorded from Jouf, averaging ≈ 346 spores 100 g−1 dry soil, and the lowest from Uqair, averaging ≈ 96 spores 100 g−1 dry soil. A total of 25 AMF species were identified, belonging to eight identified genera viz., Acaulospora, Diversispora, Gigaspora, Scutellospora, Claroideoglomus, Funneliformis, Glomus, and Rhizophagus and five families. Of the total identified species, 52% belonged to the family Glomeraceae. Moreover, the highest number of species was isolated from the sabkha in Qasab. Additionally, Glomeraceae was abundant in all the studied locations with the highest relative abundance in Uqair (48.34%). AMF species Claroideoglomus etunicatum, Funneliformis mosseae, Glomus ambisporum, and Rhizophagus intraradices were the most frequently isolated species from all the Sabkha locations with isolation frequency (IF) ≥ 60%, and Claroideoglomus etunicatum (IF ≥ 50%) was the dominant species in all the studied locations. Furthermore, data on the Shannon–Wiener diversity index showed that the highest AMF species diversity was in Qaseem and Qasab habitats. The highest Pielou’s evenness index was recorded in Jouf. Moreover, the soil parameters that positively affected the diversity of identified species included Clay%, Silt%, HCO31−, OM, MC, N, and P, while some soil parameters such as EC, Na+, SO42-, and Sand% had a significant negative correlation with the isolated AMF species. This study revealed that AMF can adapt and survive the harshest environments, such as hypersaline sabkhas, and thus can prove to be a vital component in the potential restoration of salinity-inflicted/degraded ecosystems.

Simultaneously Improving Soil AMF Community and Phosphorus Uptake by Cotton Plants by Continuous Straw Incorporation and Optimizing Phosphorus Management

ABSTRACTPhosphorus (P) fertilization and straw incorporation are essential agricultural practices, both of which have an influence on the abundance and diversity of the indigenous arbuscular mycorrhizal fungal (AMF) community. However, there are still research gaps on the interactive effects of P fertilizer application and straw incorporation on indigenous AMF in coastal saline–alkali land. Thus, this study aimed to assess several mycorrhizal features, the abundance, and the community composition of soil AMF in a field after a 4‐year barley–cotton rotation. We also explored the roles of AMF play in enhancing cotton P uptake. The field was treated with five triple superphosphate levels (0, 22, 44, 66, and 88 kg ha−1) and subjected to either straw incorporation or removal. The total P content of cotton increased with straw incorporation and increasing P application rates; however, the percentage of P partitioned to bolls, the boll capacity of the root system (BCR), and the boll loading of the root system (BLR) exhibited a trend of initially increasing followed by a decrease. The highest value was achieved when straw incorporation was combined with 44 kg P ha−1. Besides, the root colonization by AMF, soil AMF abundance, and spore density were highest at 22 kg P ha−1 without straw and 44 kg P ha−1 in the straw incorporation treatment. Redundancy analysis (RDA) revealed that the observed community shift was primarily driven by the enhancement of rhizosphere soil pH and organic matter following straw incorporation. Overall, these findings showed that straw incorporation coupled with moderate P fertilizer created a favorable rhizosphere soil environment, which, in turn, enhanced rhizosphere soil AMF diversity and abundance, thereby improving cotton root production capacity and facilitating P distribution to reproductive organs. Our results suggested that the threshold of P fertilizer input can provide a basis for saving P fertilizer and effectively maintain agricultural ecosystems' AMF diversity and abundance in coastal saline–alkali land.

Terroir and farming practices drive arbuscular mycorrhizal fungal communities in French vineyards.

Nature-based management of vineyards is at the heart of a sustainable development for the next decades. Although much is known about grapevine benefits from Arbuscular Mycorrhizal Fungi (AMF), little is known about the influence of vineyard terroir and farming practices on AMF communities. We examined the relative effect of wine terroir and agricultural practices (organic, conversion, and conventional) on AMF abundance and diversity across 75 vineyards distributed over 14 wine terroirs in 6 winegrowing regions in France. We estimate AMF abundance by measuring spore density and root mycorrhization rates, and characterize AMF communities composition using metabarcoding by sampling both root and spore compartments for each vineyard. Organic farming slightly increases AMF abundance (spore density and mycorrhization rate). Vineyards under conversion and using organic practices display a higher AMF diversity than conventional ones. Terroirs vary widely in terms of AMF abundance and diversity, with the median of OTUs count per sample ranging from 9 (Côte des Blancs) to 35 (Gigondas). The composition of AMF communities is structured mainly by terroir and in a lesser extent by practice. The effect of terroir on AMF communities is partially explained by distance decay and soil properties, but the majority of variation is still explained only by the terroir identity. Organic practices improve both abundance and diversity of AMF in vineyards, possibly leading to more productivity and resilience of grapevines. This large-scale study highlights the importance of terroir in our understanding of vineyard microbiome and paves the way to incorporation of AMF in microbial terroir studies and applications.

Higher temperatures decreased the abundance of arbuscular mycorrhizal fungi and the complexity of their networks by reducing tree diversity

Higher temperatures decreased the abundance of arbuscular mycorrhizal fungi and the complexity of their networks by reducing tree diversity

Interaction between arbuscular mycorrhizal fungi and dark septate endophytes in the root systems of Populus euphratica and Haloxylon ammodendron under different drought conditions in Xinjiang, China.

Arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) are known to enhance the tolerance of host plants to biotic and abiotic stresses, but the mechanism of their interaction under natural conditions has not been extensively studied. We analyzed the endophytic fungal diversity and colonization characteristics in the typical desert plants Populus euphratica and Haloxylon ammodendron and the relationship between them and environmental factors. Except for DSE in the roots of H. ammodendron, the colonization rates of AMF and DSE were significantly positively correlated with drought severity. The abundance of AMF and DSE under medium and mild drought conditions was greater than that under severe drought conditions. The root colonization rate and abundance of AMF were lower than those of DSE under the same drought conditions. The species diversity and abundance of AMF and DSE in P. euphratica were greater than those in H. ammodendron. AMF were more susceptible to soil factors such as soil water content, soil nitrogen and phosphorus content, and urease, whereas DSE were more affected by pH. Drought stress has different effects on AMF and DSE in the roots of P. euphratica and H. ammodendron. DSE have a greater advantage in extremely arid environments. This study demonstrates the interaction between AMF and DSE with the host plants P. euphratica and H. ammodendron as well as their effects on the adaptation of host plants to the desert environment, which can provide a basis for strengthening desert vegetation management.

Temporal effects of road disturbance on the spread of non‐native plants and arbuscular mycorrhizal fungi in subarctic mountain ecosystems

Roads in cold climate mountains are known to be important vectors in the introduction and spread of non‐native plant species. In the same context, mycorrhizal fungi communities are altered by roads, with a known positive effect on arbuscular mycorrhizal (AM) fungi diversity and abundance in disturbed roadsides. However, to what degree these two effects of roads are intertwined and how they are evolving over time is not well understood. In this study we conducted repeated surveys of non‐native plants and AM fungi between 2012 and 2022, in the northern Scandes mountains to investigate temporal changes and interactions between roads, mycorrhizal fungi and non‐native plants. We found that the upward spread of non‐native plants and lateral spread away from the roadside into the natural vegetation were so far extremely limited, with only two out of 23 non‐native species showing an increase in their upper elevational limit. However, non‐native plant species cover did increase over the ten year period, especially at lower elevations, and non‐native richness increased from 17 to 23 species. Likewise, we saw an increase in AM fungal abundance over the last four years along the roadsides at lower elevations. Furthermore, our results suggest that increases in non‐native species are unlikely to be the driving cause of the observed increase in AM fungal abundance, as AM fungi colonization varied independently of non‐native species cover dynamics.

Digging deeper into the impacts of different soil water systems on the date palm root architecture and associated fungal communities

In arid regions, excessive water use threatens agricultural sustainability and overall livelihoods. It is essential to minimize water consumption to address these issues. Date palm (Phoenix dactylifera L.) is an emblematic crop of arid regions and a major water consumer. Adapting current irrigation systems to be more water-efficient systems could help cope with the water consumption of this crop. Microbial communities associated with plants are essential for agricultural sustainability and could improve the water use efficiency in regions threatened by water scarcity. These communities should thus be seriously taken into account when adapting agrosystems to the current global change setting. However, no information is presently available on the effects of the different soil water systems on date palm microbial communities. This study highlights the impact of different soil water systems (flooding and drip irrigation, natural conditions and abandoned farms) on date palm root fungal communities at different soil depths (40, 80 and 140 cm deep). The findings revealed that the soil water systems had a marked impact on fungal communities and that drip irrigation reduced the fungal diversity but increased the abundance of arbuscular mycorrhizal fungi. We showed that these effects were similar at all sampling depths. Finally, as the root architecture is a major determinant of water uptake, we reveal different behaviors of the root architecture under these different soil water systems to 160 cm depth. The findings of this study give new insights into the date palm root architecture and associated fungal communities, particularly in the context of the water availability crisis, which drives the adaptation of agricultural systems.

Grazing increases the complexity of networks and ecological stochastic processes of mycorrhizal fungi.

Grazing increases the complexity of networks and ecological stochastic processes of mycorrhizal fungi.

Root separation modulates AMF diversity and composition in tomato-potato onion intercropping systems.

Plant-plant interactions shape arbuscular mycorrhizal fungi (AMF) communities in rhizosphere soil, with tomato/potato-onion intercropping emerging as a promising agro-ecological strategy to optimize resource utilization. However, the role of root separation methods in modulating AMF diversity within intercropping systems remains unclear. Specifically, whether the AMF community in the rhizosphere of tomato and potato-onion intercropping differs from monoculture and how root separation methods modulate these effects. This study evaluates the effects of various root separation methods (no separation, 0.45 μm nylon membrane, 38 μm nylon membrane, and solid separation) on AMF diversity and composition in tomato/potato-onion intercropping and monoculture systems. High-throughput Illumina MiSeq sequencing was used to assess AMF diversity indices (Ace, Chao1, Shannon, and Simpson), and Principal Coordinate Analysis evaluated community structure. Results showed that the non-separation mode achieved the highest Ace and Chao1 indices, indicating greater richness, while intercropping lowered Shannon and Simpson indices. Intercropping significantly reduced Glomerales but increased Paraglomerales, under the non-separation mode. Similarly, it decreased Glomus while increasing Paraglomus in the rhizosphere of both crops. Principal Coordinate Analysis revealed that root separation distinctly altered AMF community structure, reflecting specific barrier effects on AMF interactions. Intercropping increased AMF abundance in the tomato rhizosphere but reduced it in potato-onion as shown by 18S rRNA gene abundance. These findings emphasize that minimizing root separation in intercropping enhances AMF diversity and functionality, providing valuable insights for sustainable agricultural management. Understanding the role of root interactions in shaping AMF communities can help optimizing intercropping strategies to improve soil health and nutrient dynamics.

Diversity of Arbuscular Mycorrhizal Fungi Increases with Tree Age in Karstic Rocky Desertification Areas of Southwestern China

The diversity of arbuscular mycorrhizal fungi (AMF) is a crucial indicator for determining the productivity of forest ecosystems and for assessing degraded areas. At present, the effect of tree age and vegetation restoration strategies on AMF diversity in karstic rocky desertification areas remains unclear. This study investigated AMF diversity and abundance in soils planted with Delavaya toxocarpa Franch. for 18, 11, and 4 years in a karstic desertification area of southwestern China. Additionally, it explored AMF community composition in soils of an 18-year-old D. toxocarpa plantation, a secondary forest naturally restored since 2005, and an abandoned land with no human intervention. High-throughput sequencing revealed that the mean Chao1 and richness indices of AMF increased with tree age, as indicated by the highest AMF α-diversity in 18-year-old plantations. The various vegetation restoration strategies resulted in significant differences in AMF abundance and evenness indices. Although no significant differences (p = 0.33) were found between the different restoration strategies, the AMF α-diversity index showed a decreasing trend from plantation forest to secondary forest and then to abandoned land. Overall, soil organic carbon (SOC), total nitrogen (TN), and available phosphorus (AP) significantly influence AMF diversity. Additionally, soil TN, AP, hydrolysable nitrogen (HN), and urease activity (URE) shape AMF community composition. These properties varied with tree age and vegetation restoration strategies. Our findings point to good recovery results of artificial afforestation in karstic rocky desertification areas. The process accelerates vegetation restoration and enhances the mutually beneficial relationship between vegetation and AMF compared with natural restoration. However, the tree age selected in this study only represents the forest stands before mature forests, and the microbial diversity and structure in karst rocky desertification soils after mature and over-mature forest stands remain to be studied.

Influence of Mikania micrantha Kunth Flavonoids on Composition of Soil Microbial Community

Mikania micrantha, one of the world’s most destructive invasive species, is known for causing significant ecological and economic harm. While extensive research has focused on its growth characteristics, secondary metabolites, and control measures, its chemical interactions with the environment—particularly the role of flavonoids in shaping soil microbial communities—remain underexplored. In this study, we identified and quantified ten flavonoids from M. micrantha root exudates using UPLC-MS, including Hispidulin, Isorhamnetin, and Mikanin. To examine their impact, crude flavonoid extracts were applied to soil in potted experiments, which demonstrated that these compounds significantly increased soil fungal diversity and boosted the relative abundance of arbuscular mycorrhizal fungi (AMF). Furthermore, KEGG pathway analysis revealed that flavonoid addition elevated the copy numbers of genes involved in nitrogen cycling and metabolic functions, enhancing nutrient availability and microbial activity. Additionally, crude flavonoid extracts promoted the relative abundance of beneficial soil bacteria, such as Achromobacter, as well as AMF, both of which contribute to nutrient acquisition, plant growth, and soil health. These findings indicate that M. micrantha’s flavonoids can alter soil microbial community composition, thereby creating a favorable environment that reinforces its competitive edge over native plants.

Mediterranean co-living: succession of soil mycorrhizal communities associated with Halimium lasianthum shrubs

Halimium lasianthum, a widespread shrub in the western Mediterranean, uniquely co-hosts ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) fungi. Despite its ecological significance, H. lasianthum is understudied, and its mycorrhizal communities remain largely unknown. To understand the mycological ecology associated with H. lasianthum, we analyzed soil samples from intermediate and senescent understories in Ourense, northwest Iberian Peninsula. We assessed the richness, diversity, relative abundance and community composition of ECM and AM fungi. Environmental and soil variables were also examined to determine their influence on fungal distribution. Total fungal richness and abundance were higher in intermediate plots compared to senescent plots, with ECM fungi following the same trend. In comparison, AM fungal richness was higher in senescent plots (p < 0.05). ECM fungal community composition shifted with H. lasianthum age, whereas the Shannon diversity index and abundance of AM fungi remained stable. Soil pH was significantly correlated with the ECM community in intermediate plots, while the stability of the AM community was due to lower nutrient requirements and the production of resistant propagules. These findings could help to improve the management of H. lasianthum ecosystems to preserve the diversity of the mycorrhizal communities associated with this host species.

Arbuscular mycorrhizal fungi mediate soil N dynamics, mitigating N2O emissions and N-leaching while promoting crop N uptake in green manure systems

Arbuscular mycorrhizal fungi mediate soil N dynamics, mitigating N2O emissions and N-leaching while promoting crop N uptake in green manure systems

Soil amendments altered arbuscular mycorrhizal fungal communities in cadmium-contaminated vegetable fields.

Soil amendments, including various types of fertilizers, are often used to control the uptake of heavy metals such as cadmium in cropping fields. The influence of these amendments on other members of the agroecosystem, such as arbuscular mycorrhizal fungi (AMF), remains less well investigated. Here, we established an experiment with the application of woody peat organic fertilizer and phosphate rock powder to examine its effects on AMF communities in two cadmium-contaminated vegetable crop fields (cucumber and pepper). We found that the application of phosphate rock powder enhanced soil phosphorus content, while the application of woody peat organic fertilizer enhanced soil nitrogen content, but neither influenced AMF abundance. We also found little influence of either amendment on measures of AMF diversity, except in one case where the Shannon index of diversity was lower in pepper fields amended with phosphate rock powder. We did, however, find significant shifts in the community composition and relative abundances of AMF taxa in the two vegetable fields, primarily as a result of shifts in the soil pH and nitrogen content.

Vineyard maturity increases arbuscular mycorrhizal and decreases plant pathogen fungal relative abundance in bulk soil across a 1,000 km Chilean gradient

Societal Impact StatementWine production has a significant impact on the global economy. Despite this, the microbial composition of the terroir has not been studied sufficiently, particularly in the southern hemisphere. Here, we investigated how bulk soil fungal communities are affected by several abiotic and biotic factors across 1,000 km of Chilean vineyards. We found that geographical distance was the main driver of vineyard soil fungal communities. Irrespective of variety, older vineyards host a higher relative abundance of arbuscular mycorrhizal fungi (AMF) and a lower relative abundance of plant pathogenic fungi. This result could lead to significant recommendations on when to apply AMF‐based inoculants.Summary The microbial dimension of the terroir has been increasingly recognized as an essential factor determining vineyard productivity and quality. Despite this, few studies have analyzed the factors affecting soil fungal communities of vineyards in the southern hemisphere. Across a 1,000 km gradient encompassing 34 Chilean vineyards, we investigated the effects of grapevine variety, geographical distance, maturity, and soil chemical properties on the diversity and composition of soil fungi. We implemented standard soil chemical analyses and ITS‐based DNA metabarcoding of bulk soil. We found that the total soil fungal composition was significantly affected by geographical distance but not by grapevine variety and maturity. Soil chemical dissimilarity also significantly affected soil fungal composition. When analyzing specific fungal guilds, we found a contrasting successional pattern: arbuscular mycorrhizal fungal relative abundance was significantly higher at medium and old‐maturity vineyards compared with young vineyards, on which plant pathogenic fungi had a markedly lower abundance. Our results present several caveats: the molecular marker was not the most commonly used for arbuscular mycorrhizal fungi, bulk soil was sampled (instead of roots), and all abundances were relative. Still, this study constitutes one of the most extensive studies on soil fungi – both for vineyards and Chile – and might have practical implications, as knowing the drivers of fungal and mycorrhizal biodiversity can inform agricultural management decisions.

Diversity and distribution of arbuscular mycorrhizal fungi associated with vegetable crops in Haryana, India

The optimal growth and development of many vegetable crops hinge significantly upon their reliance on Arbuscular Mycorrhizal Fungi (AMF). Understanding the AMF status of vegetable crops can assist researchers in selecting suitable strains for future experiments. Therefore, a field work was carried out to determine the species diversity and composition of AMF with fifty vegetable crops from seventeen different districts of Haryana. AMF spores were isolated and identified to evaluate AMF density, diversity, and host preference in terms of AMF species richness, abundance and frequency of occurrence. Soil conditions, land use type and its physico–chemical properties played a crucial role in regulating the uneven distribution and composition of AMF. Mycotrophic structures such as linear infection (Arum–type) to coils (Paris–type) arbuscules and vesicles were seen. Interestingly, no correlation was found between spore number and root colonization. Maximum AMF spore density, spore richness and abundance were witnessed in Zea mays and Trigonella foenum–graecum. Five plants exhibited 100% AMF colonized roots, 15 plants showed above 75% and 12 plants above 50% colonization. Soil pH 6.10 to 7.40 supported the maximal abundance and frequency of occurrence of Glomus and Acaulospora with 53 species and 18 species followed by Acaulospora (18), Sclerocystis (10), Gigaspora (5), Entrophospora (4) and Sclerocystis (4). G. mosseae was the most preferred species among vegetable crops. Members of non–mycorrhizal families lack root colonization except for Brassica campestris, B. oleracea var. botrytis and B. Rapa where 2–11% root colonization was detected. Noticing the abundant AMF diversity of vegetable crops , this investigation expands the scope of detection, selection and inoculation of vegetable crops with suitable AMF species for improving their quality and quantity.

A Treasure Trove of Urban Microbial Diversity: Community and Diversity Characteristics of Urban Ancient Ginkgo biloba Rhizosphere Microorganisms in Shanghai.

Rapid urbanization has exerted immense pressure on urban environments, severely constraining the growth of ancient trees. The growth of ancient trees is closely linked to the microbial communities in their rhizospheres, and studying their community characteristics may provide new insights into promoting the growth and rejuvenation of ancient trees. In this study, the rhizosphere soil and root systems of ancient Ginkgo biloba trees (approximately 200 years old) and adult G. biloba trees (approximately 50 years old) in Shanghai were selected as research subjects. Phospholipid fatty acid (PLFA) analysis and high-throughput sequencing were employed to investigate the diversity of microbial communities in the G. biloba rhizosphere. The results indicated that the 19 PLFA species selected to characterize the soil microbial community structure and biomass were present in the rhizosphere soil of both ancient and adult G. biloba trees. However, the total microbial biomass and the microbial biomass in the rhizosphere soil of ancient G. biloba were lower than the microbial biomass in the rhizosphere soil of adult G. biloba. The biomasses of Gram-negative bacteria (G-), arbuscular mycorrhizal fungi (AMF), and protozoans (P) were significantly different. Total phosphorus, organic matter, and pH may be the key factors influencing the soil microbial community in the rhizosphere zone of ancient G. biloba. An in-depth study of AMF showed that the roots and rhizosphere soil of G. biloba contained abundant AMF resources, which were assigned to 224 virtual taxa using the MaarjAM reference database, belonging to four orders, ten families, and nineteen genera. The first and second most dominant genera were Glomus and Paraglomus, respectively. Archaeospora and Ambispora were more dominant in the rhizosphere than the roots. Furthermore, the abundance of live AMF was significantly higher in ancient G. biloba than in adult G. biloba. Therefore, future research should focus on the improvement of soil environmental characteristics and the identification and cultivation of indigenous dominant AMF in the rhizosphere of ancient G. biloba, aiming for their effective application in the rejuvenation of ancient trees.

Microbial necromass and glycoproteins for determining soil carbon formation under arbuscular mycorrhiza symbiosis

Microbial necromass and glycoproteins for determining soil carbon formation under arbuscular mycorrhiza symbiosis

Anthoxanthum odoratum (Poaceae) as a bioindicator of arbuscular mycorrhizal fungi abundance prior to native revegetation on agricultural soils

ABSTRACT Mycorrhizal fungi, which form symbiotic associations with plant roots, can be critical to the successful establishment of plants. Up to 90% of land plant species form associations with arbuscular mycorrhizal fungi (AMF), which develop fungal structures inside host roots. Common site preparation practices prior to ecological restoration, such as soil disturbance and herbicide application, may lessen the availability of AMF, potentially jeopardising plant establishment. However, the state of the soil microbiome can be difficult to assess directly. This study examines the potential of the widespread pasture grass Anthoxanthum odoratum (Sweet Vernal, Poaceae) as a bioassay of AMF abundance in soils. We present a rapid clearing and staining protocol for visualising AMF structures in the roots of A. odoratum. We then compare % colonisation on roots of naturally established A. odoratum plants collected from undisturbed pastures vs. sprayed and tilled pastures. Roots of A. odoratum collected from sprayed and tilled pastures showed a significantly lower average AMF colonisation at 43.8% compared with 57.6% on roots from undisturbed pastures. However, all plants sourced from the sprayed and tilled pastures still showed some AMF structures. Our results strongly suggest that A. odoratum can be a useful bioindicator of AMF presence and abundance in soils, offering a promising tool for seed-based restoration on retired agricultural land.

Mixed Grazing Increases Abundance of Arbuscular Mycorrhizal Fungi in Upland Welsh Grasslands

Grasslands play a crucial role in exchanges between global ecosystems and the atmosphere and form an integral part of the agricultural industry. Arbuscular mycorrhizal fungi (AMF) are mutualistic symbionts of most grassland plant species and thereby influence the functional capacity of grassland systems. Agricultural grasslands are primarily used for livestock farming and are subjected to various management practices designed to increase production, but which also alter both plant and soil communities in the process. This research investigated the effects of a selection of management practices and environmental factors on the presence and abundance of AMF in upland Welsh grasslands. The aim was to identify how these management practices affected the abundance of AMF, assessed through microscopic observations of four AMF structures: spores, hyphae, vesicles and arbuscules. The results suggest grazing sheep and cattle together had the highest overall influence on AMF abundance compared to grazing sheep or cattle separately. High plant diversity correlated with high arbuscule and vesicle abundance, but conversely, the application of lime reduced vesicle abundance. These findings offer new insights into the effects of management practices on AMF. Mixing livestock, increasing plant diversity and reducing lime applications are shown here to improve the abundance of AMF and could, therefore, help to inform sustainable farm management decisions in the future.