Absence Of UV Radiation Research Articles

Warming and UV Radiation Alleviate the Effect of Virus Infection on the Microalga Emiliania huxleyi.

The marine microalga Emiliania huxleyi is widely distributed in the surface oceans and is prone to infection by coccolithoviruses that can terminate its blooms. However, little is known about how global change factors like solar UV radiation (UVR) and ocean warming affect the host-virus interaction. We grew the microalga at 2 temperature levels with or without the virus in the presence or absence of UVR and investigated the physiological and transcriptional responses. We showed that viral infection noticeably reduced photosynthesis and growth of the alga but was less harmful to its physiology under conditions where UVR influenced viral DNA expression. In the virus-infected cells, the combination of UVR and warming (+4°C) led to a 13-fold increase in photosynthetic carbon fixation rate, with warming alone contributing a change of about 5-7-fold. This was attributed to upregulated expression of genes related to carboxylation and light-harvesting proteins under the influence of UVR, and to warming-reduced infectivity. In the absence of UVR, viral infection downregulated the metabolic pathways of photosynthesis and fatty acid degradation. Our results suggest that solar UV exposure in a warming ocean can reduce the severity of viral attack on this ecologically important microalga, potentially prolonging its blooms.

Trimethoxy Silyl End-Capped Hyperbranched Polyglycidol/Polycaprolactone Particle Gels for Cell Delivery and Tissue Repair: Mechanical Properties, Biocompatibility, and Biodegradability Studies

This study focuses on the development of new biocompatible and biodegradable particle gel scaffolds based on PCL-HBPG/1SiHBPG triblock copolymers composed of a polycaprolactone (PCL) core and two outer blocks of trimethoxysilyl end-capped hyperbranched polyglycidol (HBPG/1SiHBPG) that have the potential to be used in soft tissue regeneration. The relationship between the gel’s composition, structure, mechanical properties, and performance has been investigated for the first time and the copolymer design parameters have been optimized. The particle gel scaffolds were formed from the concentrated dispersions of the most hydrophobic PCL-45HBPG/1SiHBPG at low temperatures, and were the result of the numerous hydrogen bonds formed from the HBPG/1SiHBPG moieties as well as the formation of siloxane crosslinks (i.e., Si–O–Si bonds). These gels were formed in the physiological temperature range. Gels with a mechanical strength that gradually increases were formed from the physically crosslinked PCL-45HBPG/1SiHBPG particles effectively and safely, in the absence of UV radiation. They feature high elasticity and undergo enzyme-triggered disassembly. The gels are biocompatible and have the potential to invoke cell attachment and differentiation in the absence of exogenous biological stimuli. A successful outcome of this study will be the prospect of a new approach for tissue regeneration that is currently not available.

525 Sunscreens on the skin microbiome: Impacts on bacterial viability in the presence and absence of ultraviolet radiation

Sunscreens are a vital tool for protecting the skin from the damaging effects of ultraviolet radiation (UVR). The microbiome of the skin may play an important but poorly understood role in the susceptibility of skin to sun damage, suggesting it to be an avenue for the enhancement of future sunscreens. Despite this, the impact of sunscreens on the skin microbiome has received little research attention. We have investigated the effect of sunscreens on the viability of skin bacteria with and without exposure to UVR.

Co-exposure to UV radiation and crude oil increases acute embryotoxicity and sublethal malformations in the early life stages of Atlantic haddock (Melanogrammus aeglefinus)

Crude oil causes severe abnormalities in developing fish. Photomodification of constituents in crude oil increases its toxicity several fold. We report on the effect of crude oil, in combination with ultraviolet (UV) radiation, on Atlantic haddock (Melanogrammus aeglefinus) embryos. Accumulation of crude oil on the eggshell makes haddock embryos particularly susceptible to exposure. At high latitudes, they can be exposed to UV radiation many hours a day. Haddock embryos were exposed to crude oil (5–300 μg oil/L nominal loading concentrations) for three days in the presence and absence of UV radiation (290–400 nm). UV radiation partly degraded the eggs' outer membrane resulting in less accumulation of oil droplets in the treatment with highest oil concentration (300 μg oil/L). The co-exposure treatments resulted in acute toxicity, manifested by massive tissue necrosis and subsequent mortality, reducing LC50 at hatching stage by 60 % to 0.24 μg totPAH/L compared to 0.62 μg totPAH/L in crude oil only. In the treatment with nominal low oil concentrations (5–30 μg oil/L), only co-exposure to UV led to sublethal morphological heart defects. Including phototoxicity as a parameter in risk assessments of accidental oil spills is recommended.

Coral recruits are highly sensitive to heavy fuel oil exposure both in the presence and absence of UV light

Oil pollution remains a prominent local hazard to coral reefs, but the sensitivity of some coral life stages to oil exposure remains unstudied. Exposure to ultraviolet radiation (UVR), ubiquitous on coral reefs, may significantly increase oil toxicity towards these critical habitat-forming taxa. Here we present the first data on the sensitivity of two distinct post-settlement life stages of the model coral species Acropora millepora to a heavy fuel oil (HFO) water accommodated fraction (WAF) in the absence and presence of UVR. Assessment of lethal and sublethal endpoints indicates that both 1-week-old and 2-month-old recruits (1-wo and 2-mo) were negatively affected by chronic exposures to HFO (7 and 14 days, respectively). Relative growth (1-wo and 2-mo recruits) and survival (1-wo recruits) at end of exposure were the most sensitive endpoints in the absence of UVR, with no effect concentrations (NEC) of 34.3, 5.7 and 29.3 μg L-1 total aromatic hydrocarbons (TAH; ∑39 monocyclic- and polycyclic aromatic hydrocarbons), respectively. On average, UVR increased the negative effects by 10% for affected endpoints, and latent effects of exposure were evident for relative growth and symbiont uptake of recruits. Other sublethal endpoints, including maximum quantum yield and tissue colour score, were unaffected by chronic HFO exposure. A comparison of putative species-specific sensitivity constants for these ecologically relevant endpoints, indicates A. millepora recruits may be as sensitive as the most sensitive species currently included in oil toxicity databases. While the low intensity UVR only significantly increased the negative effects of the oil for one endpoint, the majority of endpoints showed trends towards increased toxicity in the presence of UVR. Therefore, the data presented here further support the standard incorporation of UVR in oil toxicity testing for tropical corals.

Heterometathesis of diphosphanes (R2P-PR2) with dichalcogenides (R'E-ER', E = O, S, Se, Te).

The reactions of R2P-PR2 with R'E-ER', (where E = Se, S, O, Te) to give R2P-ER' have been explored experimentally and computationally. The reaction of Ph2P-PPh2 with PhSe-SePh gives Ph2P-SePh (1) rapidly and quantitatively. The P-P/Se-Se reaction is inhibited by the addition of the radical scavenger TEMPO which is consistent with a radical mechanism for the heterometathesis reaction. Compound 1 has been fully characterised, including by X-ray crystallography. A range of other Ar2P-SeR (R = Ph, nBu or CH2CH2CO2H) have also been prepared and characterised. The reaction of 1 with [Mo(CO)4(nbd)] (nbd = norbornadiene) gives two products which, from their characteristic 31P NMR data, have been identified as cis-[Mo(CO)4(Ph2PSePh-P)2] (8) and the mixed-donor complex cis-[Mo(CO)4(Ph2P-SePh-P)(Ph2P-SePh-Se)] (9). It is deduced that the P and Se atoms in ligand 1 have comparable capacity to coordinate to Mo(0). The reaction of Ph2P-PPh2 with PhS-SPh gives Ph2P-SPh (2) quantitatively but no reaction was observed between Ph2P-PPh2 and PhTe-TePh. Heterometathesis between Ph2P-PPh2 and tBuO-OtBu does not occur thermally but has been observed under UV irradiation to give Ph2P-OtBu along with P(V) oxidation by-products. DFT calculations have been carried out to illuminate why heterometatheses with dichalcogenides R'E-ER' occur readily when E = S and Se but not when E = O and Te. The calculations show that heterometathesis is predicted to be thermodynamically favourable for E = O, S and Se and unfavourable for E = Te. The fact that a metathesis reaction between Ph2P-PPh2 with tBuO-OtBu is not observed in the absence of UV radiation, is therefore due to kinetics.

Ag-TiO2 Nanocomposites: Visible Or Solar Light Driven Plasmonic Photocatalysis?

Background: The demand for photocatalytic processes assisted by solar radiation has stimulated the upgrading of established systems, as the semiconductor modification with noble metals. Objective: the synthesis, characterization, and photocatalytic activity evaluation of the Ag-TiO2, against sulfamethoxazole molecule, and investigate the significance of the plasmonic phenomenon in Visible (450 - 1000nm) and UV-Vis (315-800 nm) radiation. Methods: Different nanocomposites Ag/TiO2 ratios were synthesized by the deposition of Ag nanoparticles on the TiO2 surface by in-situ photoreduction, and then calcinated at 400°C for 2 hr. The chemical-physical properties of the materials were examined by UV-Vis Diffuse Reflectance (UV-Vis DR) Scanning Electronic Microscopy (SEM), Transmission Electronic Microscopy (TEM), X-Ray Energy Dispersive Spectroscopy (EDS). The experiments were conducted in a cooled photochemical reactor irradiated by halogen lamp (250W). The degradation of Sulfamethoxazole was monitored by HPLC-DAD. Results: Although the prepared photocatalysts show an intense plasmonic band centered at 500 nm, no photocatalytic activity was observed in the process assisted by artificial visible radiation (λ ≥ 450 nm). In processes assisted by artificial UV-Vis radiation, the photolysis rate of the model compound (sulfamethoxazole) was higher than the photocatalytic rate, and in the absence of UV radiation, all the reactions were inhibited. The positive effect of the presence of silver nanoparticles onto the TiO2 surface was only evidenced in studies involving solar radiation. Conclusion: The results suggest the need for a balance between UV and Vis radiation to activate the nanocomposite and perform the sulfamethoxazole degradation.

Chemical synthesis versus green synthesis to obtain ZnO powders: Evaluation of the antibacterial capacity of the nanoparticles obtained by the chemical method

In this work, zinc oxide nanoparticles (ZnO-NPs) were synthesized using both a chemical route (polymeric complex method) and a green route (controlled precipitation, using suspensions of garlic, Allium sativum, and chili, Capsicum annuum, as solvents). The obtained solids were characterized using infrared and Raman spectroscopies, X-ray diffraction (XRD) and scanning electron microscopy (SEM). While the solid synthesized by the chemical pathway presented a wurtzite-like crystalline structure of ZnO, those obtained by green synthesis were essentially amorphous. In the IR spectra of the materials synthesized by the two methods, the presence of the characteristic bands of ZnO was evidenced, as well as the active groups of garlic and chili pepper in the IR spectrum of the sample obtained by green synthesis. Something similar happened with the Raman spectra of the synthesized samples. To account for the alterations found in both structure and composition of the obtained solids, a series of reactions were proposed between the zinc precursor with both the active garlic and chili molecules present in the solvent during green synthesis. Considering the potential use of synthesized ZnO as antibacterial material, the action of ZnO-NPs (< 100 nm with a spheroidal morphology) obtained by the chemical route was evaluated on a strain of Escherichia coli in the absence of UV radiation. The results indicated that the synthesized ZnO-NPs, in high and medium concentrations (between 500 and 32 µg/mL), inhibited growth of the bacteria, from ~ 70% to ~ 50% respectively, with a minimum inhibitory concentration, MIC50, of 30.40 µg/mL

Aerosol formation in the filtered atmospheric air and regularities of their evolution

Presented is the study of atmospheric aerosol particle formation and evolution processes arising in filtered atmospheric air in complete darkness. Studies were carried out in the Large Aerosol Chamber (LAC) of RPA «Typhoon» with 3200 m3 volume under conditions close to natural atmospheric ones. The large volume of the chamber and its design features make it possible to exclude the influence of boundary conditions as well as any additional pollution sources from the chamber walls and from the equipment inside the LAC on the processes under study. We observe the formation of nanoparticles apparently due to gas-to-particle conversion in the absence of UV radiation. We investigate these newly formed particle growth regularities from 15 nm size to cloud condensation nuclei characteristic sizes under controlled conditions. The observed evolution regularities of the newly formed due to gas-to-particle conversion aerosol spectra can significantly contribute to the understanding of atmospheric aerosol formation processes responsible for cloudiness and precipitation.

Comparative sensitivity of the early life stages of a coral to heavy fuel oil and UV radiation

During an oil spill, shallow, tropical coral reefs are likely to be simultaneously exposed to high intensities of ultraviolet radiation (UVR), which can exacerbate the toxicity of petroleum oils. While successful recruitment of corals is critical for reef recovery following disturbances, the sensitivity of several early life stages of coral to petroleum hydrocarbons has not been investigated, particularly for UVR co-exposure. Here we present the first dataset on the relative sensitivity of three early life stages (gametes, embryos and planula larvae) in a model broadcast spawning coral species, Acropora millepora, to the dissolved fraction of a heavy fuel oil (HFO), both in the absence and presence of UVR. All early life stages were negatively impacted by HFO exposure but exhibited distinct sensitivities. Larval metamorphosis was the most sensitive endpoint assessed with a 10% effect concentration of 34 μg L−1 total aromatic hydrocarbons (TAH) in the absence of UVR. The impact on fertilisation success was highly dependent on sperm density, while the fragmentation of embryos masked embryo mortality. Larval metamorphosis was conclusively the most reliable endpoint for use in risk assessments of the endpoints investigated. Putative critical target lipid body burdens (CTLBBs) were calculated for each life stages, enabling a comparison of their sensitivities against species in the Target Lipid Model (TLM) database. A. millepora had a putative CTLBB of 4.4 μmol g−1 octanol for larval metamorphosis, indicating it is more sensitive than any species currently included in the TLM database. Coexposure to UVR reduced toxicity thresholds by 1.3-fold on average across the investigated life stages and endpoints. This increase in sensitivity in the presence of UVR highlights the need to incorporate UVR co-exposure (where ecologically relevant) when assessing oil toxicity thresholds, otherwise the risks posed by oil spills to shallow coral reefs are likely to be underestimated.

UV-induced citrus resistance to spider mites (Tetranychus urticae)

Under reduced solar UV radiation, such as in polycarbonate-covered nurseries, the two-spotted spider mite Tetranychus urticae is a major pest of all citrus cultivars, while under natural solar radiation, only sensitive cultivars are infested. We hypothesized that citrus resistance to T. urticae is induced by UV. We infested seedlings of Citrus volkameriana rootstock with T. urticae under natural and under UV-screened solar radiation, along with non-infested control seedlings. We then monitored the establishment of the spider mites and analyzed the volatile leaf profile using GC-MS. The density of spider mites was reduced dramatically on seedlings exposed to solar UV. Overall, ninety volatile compounds (47 monoterpenes, 35 sesquiterpenes, and 8 aldehydes) were detected in the leaves, many of them known to be herbivore-induced and/or UV enhanced. Their levels were affected not only by solar UV radiation or by mite infestation independently. Synergistic interaction of these two factors resulted in 90% of the volatiles suppressed by T. urticae infestation in the absence of UV radiation. This suggests that plant induced resistance is dependent on exposure to UV radiation.

Natural Variation among Arabidopsis Accessions in the Regulation of Flavonoid Metabolism and Stress Gene Expression by Combined UV Radiation and Cold.

Plants are constantly exposed to stressful environmental conditions. Plant stress reactions were mainly investigated for single stress factors. However, under natural conditions plants may be simultaneously exposed to different stresses. Responses to combined stresses cannot be predicted from the reactions to the single stresses. Flavonoids accumulate in Arabidopsis thaliana during exposure to UV-A, UV-B or cold, but the interactions of these factors on flavonoid biosynthesis were unknown. We therefore investigated the interaction of UV radiation and cold in regulating the expression of well-characterized stress-regulated genes, and on transcripts and metabolites of the flavonoid biosynthetic pathway in 52 natural Arabidopsis accessions that differ widely in their freezing tolerance. The data revealed interactions of cold and UV on the regulation of stress-related and flavonoid biosynthesis genes, and on flavonoid composition. In many cases, plant reactions to a combination of cold and UV were unique under combined stress and not predictable from the responses to the single stresses. Strikingly, all correlations between expression levels of flavonoid biosynthesis genes and flavonol levels were abolished by UV-B exposure. Similarly, correlations between transcript levels of flavonoid biosynthesis genes or flavonoid contents, and freezing tolerance were lost in the presence of UV radiation, while correlations with the expression levels of cold-regulated genes largely persisted. This may indicate different molecular cold acclimation responses in the presence or absence of UV radiation.

CeO2 foam-like nanostructure: biosynthesis and their efficient removal of hazardous dye.

In this study, CeO2 (cerium oxide) nanoparticles were synthesized using Pinus halepensis pollen and were characterized by field emission scanning electron microscopy (FESEM), powder X-ray diffraction (PXRD) and Raman spectroscopy. The results showed that the ensuing CeO2 nanostructures, ranging in size from 5 to 25nm, had high porosity. Synthesized CeO2 showed the effective catalytic activity towards the photocatalytic removal of dyes. In this work, the photocatalytic activity to removal dye (methyl violet 2B), in the absence of UV radiation, using cerium dioxide nanoparticles (CeO2-NP) was determined. In this research, four main factors such as effect on color, concentration and pH were examined and maximum %R was obtained about was 97% in 75min in presence of 50mg of hydrogen peroxide.

Controlled morphological and physical properties of ZnO nanostructures synthesized by domestic microwave route

The limited applications of ZnO in the visible light are still a challenge. In a short-term reaction (15 min), controlling the growth and morphology of zinc oxide in nano-structures had been achieved using a domestic microwave as a simple, quick and inexpensive tool. Besides, no seeds substrates were needed to be immersed during preparation process. Adding capping or complexing agents helped in controlling the surface morphology of ZnO, like hexamethylenetetramine, poly(vinyl pyrrolidone) (Polyvinyl alcohol), Hydrazine hydrate, trisodium citrate and ethylenediamine. The XRD results indicated the crystalline hexagonal wurtzite phase for all ZnO samples showing crystallite size ranged from 16.8 to 31.2 nm. SEM photos for the prepared ZnO NS samples demonstrated morphologies of twin-short rod, hexagonal rod, nanowires, nanosheet, and nanoflower structures. UV–visible and PL spectroscopy for measuring the optical properties showed that the band gap of ZnO samples ranged between 3.25 and 3.21 eV and still smaller than the standard value. Sample (M2) showed the highest intensity peak at 438 nm and high broad green emission peak at 511 nm. In brief, both solvent nature and crystal growth in the specific solvents are the keys to enforcing the crystal morphology. Eventually, the antibacterial activity of zinc oxide was evaluated in the presence and absence of UV radiation. Findings showed that all aqueous solution of ZnO samples irradiated by UV exhibit more antibacterial activity due to the increased amount of H2O2 generated which is fatal to bacteria.

Application of FeCl3 and TiO2-coated algae as innovative biophotocatalysts for Cr(VI) removal from aqueous solution: A process intensification strategy

Chromium is a metal that could be both toxic and essential dependent on its oxidation state. While Cr(VI) is toxic to plants, animals and microorganisms, Cr(III) at low levels is an essential dietary nutrient for human beings. In this study, novel biophotocatalysts were developed and applied for Cr(VI) reduction and removal from aqueous solution. The biophotocatalysts were successfully prepared by the direct coating of the raw and protonated (immersion into 0.2 M HNO3) brown algae Laminaria hyperborea with FeCl3, AlCl3, and TiO2 solutions. The products of each treatment were characterized and utilized in an equilibrium study for Cr(VI) removal, which revealed a low efficiency for algae coated with aluminum. The Cr(VI) removal via the synergic effect of photocatalysis and adsorption was investigated through kinetic studies in the presence and in the absence of UV radiation using protonated algae, protonated algae coated with iron and protonated algae coated with titanium as biophotocatalysts. The results showed that the material previously protonated and coated with iron presented the best Cr(VI) removal results, reaching an efficiency removal of 100% after 90 min. The UV radiation improves the removal efficiency of the chromium species for the experiments carried out with protonated algae coated with titanium. The scale-up of the combined process was successfully performed on a radial photoreactor with a concentric UV radiation using the protonated algae coated with iron and titanium as biophotocatalysts. Protonated algae coated with iron, the Cr(VI) species disappeared completely after 120 min, while for protonated algae coated with titanium, the Cr(VI) was still in the solution even after 210 min. The reuse of the biophotocatalysts in consecutive Cr(VI) treatment cycles without regeneration was also performed, indicating that the protonated algae coated with iron have the potential to be used in as biophotocatalysts in the treatment of wastewaters contaminated with Cr(VI), reaching the restricted discharge limit of this metal.

Magnetite Synthesis in the Presence of Cyanide or Thiocyanate under Prebiotic Chemistry Conditions.

Magnetite is an iron oxide mineral component of primitive Earth. It is naturally synthesized in different ways, such as magma cooling as well as olivine decomposition under hydrothermal conditions. It is probable magnetite played a significant role in biogenesis. The seawater used in the current work contained high Mg2+, Ca2+ and SO42− concentrations, unlike the seawater of today that has high Na+ and Cl− concentrations. It is likely that this seawater better resembled the ion composition of the seas of the Earth from 4 billion years ago. Cyanide and thiocyanate were common molecules in prebiotic Earth, and especially in primitive oceans, where they could act on the magnetite mechanism synthesis via Fe2+ interaction. In this research, magnetite samples that were synthesized under prebiotic conditions in the presence of cyanide or thiocyanate, (both with and without artificial seawater), showed that, besides magnetite, goethite and ferrihydrite can be produced through different Fe2+-ion interactions. Cyanide apparently acts as a protective agent for magnetite production; however, thiocyanate and seawater 4.0 Gy ions produced goethite and ferrihydrite at different ratios. These results validate that Fe3+ oxides/hydroxides were possibly present in primitive Earth, even under anoxic conditions or in the absence of UV radiation. In addition, the results show that the composition of water in early oceans should not be neglected in prebiotic chemistry experiments, since this composition directly influences mineral formation.

Epidermal UVA screening capacity measured in situ as an indicator of light acclimation state of leaves of a very plastic alpine plant Soldanella alpina L.

Soldanella alpina differing in leaf epidermal UV-A absorbance (DEA375), as measured with the Dualex, was investigated as a model alpine plant for the flavonoid (Flav) composition and concentration and for anatomical and pigment characteristics. In sun leaves, twenty-three flavones were characterised by their mass formula, their maximum absorption, their glycosylation, their methylation and dehydroxylation pattern. The flavones belonged to four subfamilies (tetra-hydroxy-flavones, penta-hydroxy-flavones, penta-hydroxy-methyl-flavones and tri-hydroxy-di-methoxy-flavones), abundant in sun and shade leaves. Their concentration was estimated by their absorption at 350 nm after HPLC separation. Sun leaves contained relatively higher contents of penta-hydroxy-methyl-flavones and shade leaves higher contents of tetra-hydroxy-flavones. The flavones were present mainly in vacuoles, all over the leaf. After shade-sun transfer, the content of most flavones increased, irrespective of the presence or absence of UV radiation. Highly significant correlations with the log-transformed DEA375 suggest that DEA375 can be readily applied to predict the flavone content of S. alpina leaves. Shade-sun transfer of leaves decreased the hydroxycinnamic acid (HCA) content, the mass-based chlorophyll (Chl) a+b content and the Chl/Carotenoid (Car) ratio but increased DEA375, and the Car content. Together with previously reported anatomical characteristics all these parameters correlated significantly with the DEA375. The Flav content is therefore correlated to most of the structural characteristics of leaf acclimation to light and this can be probed in situ by DEA375.

Regulation of Immune Activation by Optical Control of TLR1/2 Heterodimerization.

The activation of toll-like receptors (TLRs) plays important roles in the immune response. The ability to control the activities of TLRs could be usable as a switch for immune response. Here we have rationally designed and synthesized a photoswitchable Pam3 CSK4 derivative-P10-to control the activation of TLR1/2. The ground-state trans-P10 was able to stimulate and activate antigen-presenting cells (APCs) by promoting TLR1/2 heterodimerization. However, cis-P10, derived from UV irradiation of trans-P10, reduced the activities of APCs by impeding the TLR1/2 heterodimerization. In the absence of UV radiation, the cis-P10 slowly returned to its ground trans state, restoring the activities of the APCs stimulation. Our results indicated that optical control of TLR1/2 heterodimerization mediated by the photoswitchable P10 offers the potential to regulate immune activation and inflammation.

Removal of Rhodamine 6G in the absence of UV radiation using ceria nanoparticles (CeO2-NPs)

In this work, the capacity to removal rhodamine 6 G (R6 G), in the absence of UV radiation, using cerium dioxide nanoparticles (CeO2-NP) was determined. The synthesized NPs were characterized by X-ray powder diffraction (XRD) and X-ray photoelectron (XPS), Raman and photoluminiscence spectroscopies. The results indicated that CeO2 powders had a crystallite size between ∼ 12 and ∼ 14 nm. The presence of the peroxide species (O22-) η2, superoxide (O2-) η1, adsorbed species O2δ (0 < δ < 1) over the surface of the ceria nanoparticles was demonstrated in the Raman spectra. Additionally, the ability of the CeO2-NPs synthesized to interact with organic molecules was determined by evaluating the removal of the R6 G dye. The results of these tests showed substantial dye removal by the as-synthesized CeO2-NPs at pH∼9.1 (∼87 % within 120 min). The kinetic adsorption of the R6 G by these NPs was suitably described by a pseudo-second-order equation.

The antagonistic effect of UV radiation on warming or nitrate enrichment depends on ecotypes of freshwater macroalgae (Charophytes).

Increases in ultraviolet radiation (UVR), a negative global change factor, affect aquatic primary producers. This effect is expected to be modulated by other global change factors, and to be different for populations adapted to different environments. A common garden experimental approach using freshwater green macroalgae, the cosmopolitan charophyte species Chara hispida and C.vulgaris, allowed us to test whether the beneficial increases in water temperature (T) and nitrate concentration (N) mitigate negative UVR effects. Also, whether these interactions would be not only species-specific but also according to the origin of the population; therefore, two populations of each species were used: one from a coastal wetland and the other from a mountain lake. Two factorial-design experiments were performed: (i) the presence and absence of UVR×lower and higher T×four populations, and (ii) the presence and absence of UVR×lower and higher N×four populations. Response variables were: growth, morphometry, UVR-protective compounds, photosynthetic pigments, and stoichiometric composition. There were consistent response patterns in the key variables that represent different organization levels. Our main results showed that both warming and, to a lesser extent, the increase in nutrients ameliorated the negative effects of UVR on the molecular processes involved in acclimation to UVR, and that such a mitigating effect depended on the different phenotypic plasticity of each species and each ecotype. The coastal populations, being from a more variable environment, were more resilient than the mountain populations, mainly because of changes in growth and morphology.