Ability Of Mineral Trioxide Aggregate Research Articles

Evaluation of sealing ability of Biodentine™ and mineral trioxide aggregate in primary molars using scanning electron microscope: A randomized controlled in vitro trial

Objective:The aim of this study was to compare the sealing ability of mineral trioxide aggregate (MTA) and Biodentine™ when used to repair the furcal perforations in primary molars using scanning electron microscope (SEM).Study Design:The study sample comprised forty recently extracted primary molars. These teeth were placed in a 5.25% sodium hypochlorite solution for 24 h and washed with tap water. Access cavities were made using a round bur in high-speed handpiece. Perforations were made in the center of the floor of the pulpal chamber using a 0.5 mm round bur. The teeth were randomly assigned into two experimental groups based on the material used to seal the perforation: Group A – MTA and Group B – Biodentine™. The packed materials were allowed to set for 24 h. The samples were sectioned longitudinally and the extent of marginal adaptation was measured by SEM. Wilcoxon-signed rank test was used for statistical analysis using SPSS software.Results:All teeth exhibited microleakage, but Biodentine™ showed significantly less leakage (0.149) compared to MTA (0.583).Conclusion:Based on the results of this study, Biodentine™ showed lesser microleakage compared to MTA and thus may be a good alternative to MTA.

Effect of methods of evaluation on sealing ability of mineral trioxide aggregate apical plug

Aim:The purpose of the study was to evaluate and compare the sealing ability of mineral trioxide aggregate (MTA) with three different methods.Materials and Methods:Forty single canal teeth were decoronated, and root canals were enlarged to simulate immature apex. The samples were randomly divided into Group MD = MTA-angelus mixed with distilled water and Group MC = MTA-angelus mixed with 2% chlorhexidine, and apical seal was recorded with glucose penetration method, fluid filtration method, and dye penetration methods and compared.Results:The three methods of evaluation resulted differently. The glucose penetration method showed that MD sealed better than MC, but difference was statistically insignificant (P > 0.05). The fluid filtration method resulted that Group MC was statistically insignificant superior to Group MD (P > 0.05). The dye penetration method showed that Group MC sealed statistically better than Group MD.Conclusion:No correlation was found among the results obtained with the three methods of evaluation. Addition of chlorhexidine enhanced the sealing ability of MTA according to the fluid filtration test and dye leakage while according to the glucose penetration test, chlorhexidine did not enhance the sealing ability of MTA. This study showed that relying on the results of apical sealing by only method can be misleading.

Sealing ability of mineral trioxide aggregate Plus™ and Biodentine™ for repair of furcal perforation in primary molars: An in vitro study.

Background:One of the unfavorable outcomes of endodontic treatment in primary molars is furcal perforation. During treatment, bacterial infection at the site of perforation should be prevented for better prognosis.Aim:This study aims to compare sealing ability of mineral trioxide aggregate (MTA) Plus™ and Biodentine™ for the repair of furcal perforation in primary molars using spectrophotometry.Materials and Methods:Access opening was done for all ninety extracted teeth. Perforation was made in furcation area in all the teeth. The sample size consisted of ninety extracted teeth. They were divided into four groups, Group 1 (n = 30) in which perforations were repaired with MTA Plus™, Group 2 (n = 30) in which perforations were repaired with Biodentine™. The other two groups were considered as control groups, Group 3 (n = 15) in which perforations were left unsealed (positive control) and Group 4 (n = 15) without perforations (negative control). Dye extraction method was used to compare the sealing ability of MTA Plus™ and Biodentine™. Statistical analysis was done using ANOVA test to compare the mean between the different groups. Intergroup comparison was performed using post hoc Scheffe test.Results:The highest dye absorbance was seen in the positive control group with a mean value of 0.080 ± 0.033. The mean value of MTA Plus™ was 0.031 ± 0.026 and Biodentine™ was 0.024 ± 0.031.Conclusion:The mean value of dye absorption of MTA Plus™ was greater than Biodentine™ but it was statistically insignificant.

An in vitro Comparative Evaluation of the Sealing Ability of Mineral Trioxide Aggregate with Different Mixing Reagents

ABSTRACT Background Mineral trioxide aggregate (MTA) exhibits several properties of an ideal root-end filling. It, however, has certain disadvantages such as long setting time, difficult handling characteristics, and limited antimicrobial activity. In order to improve its properties, several commonly used reagents have been used instead of sterile water. Hence the objective of this study was to evaluate the root-end sealing ability of white MTA combined with either distilled water, 0.12% chlorhexidine solution, 10% doxycycline solution, 3% sodium hypochlorite solution, or 10% calcium chloride solution using a bacterial leakage test. Materials and methods Seventy-two extracted, decoronated human maxillary incisor teeth were used. After cleaning and shaping of the canals, root-end resection was performed at 90°. Root-end cavities were filled with ProRoot MTA combined with the test reagents. Microleakage was assessed using a bacterial leakage test for a period of 60 days. Results were analyzed using the log rank test. The level of significance was 0.05. Results There was a significant increase in the sealing ability when MTA was mixed with calcium chloride, sodium hypochlorite, or doxycycline when compared with distilled water or chlorhexidine. Also, MTA combined with calcium chloride demonstrated a higher probability of surviving 60 days. Conclusion The sealing ability of MTA was improved when combined with calcium chloride, sodium hypochlorite, and doxycycline. How to cite this article Dsouza T, Bhandary S, Srinivasan R. An in vitro Comparative Evaluation of the Sealing Ability of Mineral Trioxide Aggregate with Different Mixing Reagents. World J Dent 2016;7(2):54-58.

Apical Sealing Ability of Mineral Trioxide Aggregate, Intermediate Restorative Material and Calcium Enriched Mixture Cement: A Bacterial Leakage Study.

Introduction:This in vitro study compared the apical sealing ability of three common root end filling materials namely mineral trioxide aggregate (MTA), intermediate restorative material (IRM) and calcium-enriched mixture (CEM) cement using a bacterial leakage model.Methods and Materials:The study was conducted on 83 single-rooted human teeth. Tooth crowns were cut and root canals were prepared using the step-back technique. Apical 3 mm of the roots were cut and a three-mm-deep cavity was prepared using an ultrasonic instrument. The samples were divided into three groups (n=25) according to the root-end filling material including MTA, IRM and CEM cement. The roots were inserted into cut-end microtubes. After sterilization with ethylene oxide, microtubes were placed in sterile vials containing 10 mL of Brain Heart Infusion (BHI) broth and incubated at 37°C and 0.1 mL of Enterococcus faecalis suspension compatible with 0.5 McFarland standard (1.5×108 cell/ ml), which was refreshed daily. This procedure was continued for 70 days. The data were analyzed using the chi-square, Kruskal-Wallis and log rank tests. The level of significance was set at 0.05. Results:No significant difference was found in bacterial microleakage among three groups; MTA showed slightly (but not significantly) less microleakage than IRM and CEM. However, the difference in the mean time of microleakage was significant among the groups (P<0.04) and in MTA samples leakage occurred in a longer time than CEM (P<0.012). Conclusion:The three tested root end filling materials had equal sealing efficacy for preventing bacterial leakage.

Comparison of the Root End Sealing Ability of Four Different Retrograde Filling Materials in Teeth with Root Apices Resected at Different Angles - An Invitro Study.

Insufficient apical seal is the significant reason for surgical endodontic disappointment. The root-end filling material utilized should avoid egress of potential contaminants into periapical tissue. The aim of this study was to compare the sealing ability of four root-end filling materials MTA, Portland cement, IRM, RMGIC in teeth with root apices resected at 0 and 45 angle using dye penetration method under fluorescent microscope. Hundred extracted human maxillary anterior teeth were sectioned horizontally at the cement-enamel junction. After cleaning, shaping and obturation with gutta-percha and AH Plus sealer, the tooth samples were randomly divided in two groups (the root apices resected at 0° and 45° to the long axis of the root). The root resections were carried out by removing 2 mm and 1 mm in both the groups. Following which 3 mm deep root-end cavities were prepared at the apices and the root were coated with nail varnish except the tip. The teeth in both the group were randomly divided into four subgroups each (Pro root MTA, Portland cement, IRM and Light cure nano GIC Ketac N-100). All the retrofilled samples were stored in acrydine orange for 24 hours after which they were cleaned and vertically sectioned buccolingually. The sectioned root samples were observed under fluorescent microscope. The root apex sealing ability of Mineral Trioxide Aggregate (MTA) was superior to Portland cement, Intermediate Restorative Material (IRM) and LC GIC. IRM demonstrated the maximum apical leakage value among all the materials. Portland cement and LC GIC showed comparable sealing ability. The angulation whether 0° or 45° angle did not affect the sealing ability of all the four materials used, MTA proved to be one of the superior materials for root-end filling.

Bacterial Leakage of Mineral Trioxide Aggregate, Calcium-Enriched Mixture and Biodentine as Furcation Perforation Repair Materials in Primary Molars.

Introduction:Adequate seal of iatrogenically perforated area within the root canal system can improve the long term treatment prognosis. This in vitro study evaluated the sealing ability of mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) cement and Biodentine in repair of furcation perforation in primary molars. Methods and Materials:A total of 61 freshly extracted primary mandibular second molars were randomly divided into three groups (n=17) and 10 teeth were put in negative (without perforation, n=5) and positive (perforated without repair, n=5) control groups. Turbidity was used as the criteria of bacterial leakage, when detected in the model of dual-chamber leakage. Data were analyzed using the Chi-Square and Kaplan-Meier survival analysis in SPSS software. The level of significance was set at 0.05. Results:All positive samples showed turbidity, whereas none of the negative samples allowed bacterial leakage. There was no significant difference between the number of turbidity samples in repaired teeth with all test materials (P=0.13). No significant difference was also detected in the mean survival time (P>0.05). Conclusion:CEM cement and Biodentine showed promising results as perforation repair materials and can be recommended as suitable alternatives of MTA for repair of furcation perforation of primary molars.

Scanning electron microscopy (SEM) evaluation of sealing ability of MTA and EndoSequence as root-end filling materials with chitosan and carboxymethyl chitosan (CMC) as retrograde smear layer removing agents.

Aim:The purpose of this study was to evaluate the sealing ability of mineral trioxide aggregate (MTA) and EndoSequence with chitosan and carboxymethyl chitosan (CMC) as retrograde smear layer removing agents using scanning electron microscopy (SEM).Materials and Methods:Forty human single rooted teeth were taken. Crowns were decoronated and canals were obturated. Apically roots were resected and retrograde cavities were done. Based on the type of retrograde material placed and the type of smear layer removal agent used for retrograde cavities, they were divided into four groups (N = 10): Group I chitosan with EndoSequence, group II chitosan with MTA, group III CMC with EndoSequence, and Group IV CMC with MTA. All the samples were longitudinally sectioned, and the SEM analysis was done for marginal adaptation.Statistical Analysis:Kruskal-Wallis and Mann-Witney analysis tests.Results:SEM images showed the presence of less gaps in group III, i.e., CMC with EndoSequence when compared to other groups with statistically significant difference.Conclusion:Within the limited scope of this study, it was concluded that EndoSequence as retrograde material showed better marginal sealing ability.

Análise da capacidade seladora dos cimentos de Portland e agregado de trióxido mineral em perfurações de furca de molares

Objective: To analyze the sealing ability of Mineral Trioxide Aggregate (MTA) and Portland Cement (PC) in furcation perforations of human molars through three techniques. Methods: Ninety upper and lower extracted human molars with formed roots and not fused were selected. Perforations were performed on the floors of pulpal chambers by means of spherical burs. Specimens were randomly divided into two experimental groups of forty-five teeth each, using PC for Group I and white MTA for Group II. Group I was divided into 3 subgroups, called IA, IB, IC with fifteen samples each. Group II was also divided into 3 subgroups, called IIA, IIB, IIC with fifteen samples each. The checks of infiltration were carried out through a stereoscope with 5X magnification. The Kruskall-Wallis and Mann-Whitney tests were used for statistical analysis. Results: The use of MTA and PC inserted by three different techniques did not prevent the occurrence of leakage into furcation of extracted human molars. It is also clear that the CP accommodated in furcation perforations through ultrasound had better performance. Conclusion: Further studies should be carried out to improve the performance of the sealing material for application in critical dental regions.

A Confocal Laser Scanning Microscopic Study Evaluating the Sealing Ability of Mineral Trioxide Aggregate, Biodentine and Anew Pulp Capping Agent-Theracal

Abstract Aims and Objectives: Viability and health of the pulpal tissue after an exposure can be stimulated with biocompatible pulp capping materials. Mineral trioxide aggregate(MTA), though biocompatible with excellent sealing ability, has clinical disadvantages like poor handling properties and long setting time. New pulp capping agents- Biodentine and Theracal have proven to overcome MTA’s shortcomings. This study aimed to comparatively evaluate the sealing ability of Mineral Trioxide Aggregate(Angelus), Biodentine (Septodont) and Theracal(Bisco) when used as pulp capping agents. Materials and Methods: Occlusal cavities were prepared in extracted human third molars. The cavities were divided into 3 groups containing 12 samples each. Pulp capping in samples of group I, II and III was done with MTA, Biodentine and Theracal respectively. All samples were kept in a damp environment for 24 hrs. In each tooth, the root was cut just below the cementum-enamel junction. An aqueous 0.25% solution of Rhodamine-B was put in the open pulp chambers of all the samples and placed upside-down in a damp environment. The dye was left for 3 hrs to permeate toward the interface. After being stained, all samples were rinsed with distilled water and sectioned vertically. A Confocal Laser Scanning Microscope was used to image the samples. Results: No significant difference was found in interfacial microleakage between MTA and Biodentine. Theracal exhibited less interfacial microleakage than the two. Conclusion: Biodentine and MTA exhibit an efficient and durable protection of pulp. Theracal exhibits better sealing ability as a pulp capping agent than MTA and Biodentine.

Sealing Ability of Mineral Trioxide Aggregate and Glass Ionomer Cement as Root-end Filling Materials

Sealing Ability of Mineral Trioxide Aggregate and Glass Ionomer Cement as Root-end Filling Materials

Evaluation of Sealing Ability of Three Root-end Filling Materials

Introduction: Reinfection of root canal treated tooth leads to endodontic failure. In such case surgical endodontic therapy is the treatment of choice which provides obturation of radicular space with 3-dimensional sealing.Objective: To evaluate and compare the sealing ability of Mineral trioxide aggregate (MTA) with Glass ionomer cement (GIC) and Super EBA (Epoxybenzoic acid) root-end filling materials.Materials &amp; Method: 94 extracted single rooted upper anterior teeth were selected for root canal preparation and obturation. Root were apically resected 3 mm; and 3 mm deep Class-I root-end cavities were prepared. Teeth were randomly distributed into 3 groups comprising 30 teeth in each group. Group-1 was retrofilled with MTA, Group-2 with GIC and Group-3 with Super EBA. 2 positive and 2 negative control teeth were instrumented and obturated but not filled on root-end cavity. In experimental groups and positive control group the whole surface of teeth except 3 mm adjacent to resected area were covered with twocoats of nail polish but in negative control group whole surface of teeth were covered, dried, immersed in 1% methylene blue for 72 hours. Each root was sectioned labio-lingually to the long axis of root. Depth of dye penetration was evaluated by 10x stereo-microscope.Result: MTA retrograde filling showed the best hermetic seal property whereas, few GIC and Super EBA samples showed microleakage in retrograde filling. GIC showed better sealing ability than Super EBA. Conclusion: MTA has potential to provide better hermetic-seal than GIC and Super EBA as root-end filling material.

Effect of post space preparation on the sealing ability of mineral trioxide aggregate and Gutta-percha: A bacterial leakage study.

Aim:The purpose of this study was to evaluate the effect of post space preparation on the sealing ability of teeth obturated with mineral trioxide aggregate (MTA) and Gutta-percha.Materials and Methods:Fifty intact human mandibular premolars, after decoronation and biomechanical preparation, were randomly divided into three experimental groups. In Group A (n = 10), the canals were obturated with Gutta-percha using cold lateral compaction technique, followed by immediate post space preparation. In Group B (n = 10), the canals were obturated with 8 mm of MTA and in Group C (n = 10), sectional obturation with 4 mm of MTA was done, followed by delayed post space preparation. All specimens were subjected to bacterial leakage analysis, and the occurrence of turbidity was checked.Results:All specimens in the lateral compaction group leaked. The mean number of days at which leakage was observed in Groups A, B and C were 18.5 days, 93.6 days and 95.5 days.Conclusions:MTA can be considered as an alternative to Gutta-percha as an apical third restorative material for teeth indicated for post and core, as it demonstrates better-sealing ability compared with Gutta-percha. Further, the removal of set MTA for post space preparation does not disrupt the integrity of the remaining apical MTA.

Sealing ability of three root-end filling materials prepared using an erbium: Yttrium aluminium garnet laser and endosonic tip evaluated by confocal laser scanning microscopy.

Aims:(1) To compare the sealing ability of mineral trioxide aggregate (MTA), Biodentine, and Chitra-calcium phosphate cement (CPC) when used as root-end filling, evaluated under confocal laser scanning microscope using Rhodamine B dye. (2) To evaluate effect of ultrasonic retroprep tip and an erbium:yttrium aluminium garnet (Er:YAG) laser on the integrity of three different root-end filling materials.Materials and Methods:The root canals of 80 extracted teeth were instrumented and obturated with gutta-percha. The apical 3 mm of each tooth was resected and 3 mm root-end preparation was made using ultrasonic tip (n = 30) and Er:YAG laser (n = 30). MTA, Biodentine, and Chitra-CPC were used to restore 10 teeth each. The samples were coated with varnish and after drying, they were immersed in Rhodamine B dye for 24 h. The teeth were then rinsed, sectioned longitudinally, and observed under confocal laser scanning microscope.Statistical Analysis Used:Data were analyzed using one-way analysis of variance (ANOVA) and a post-hoc Tukey's test at P < 0.05 (R software version 3.1.0).Results:Comparison of microleakage showed maximum peak value of 0.45 mm for Biodentine, 0.85 mm for MTA, and 1.05 mm for Chitra-CPC. The amount of dye penetration was found to be lesser in root ends prepared using Er:YAG laser when compared with ultrasonics, the difference was found to be statistically significant (P < 0.05).Conclusions:Root-end cavities prepared with Er:YAG laser and restored with Biodentine showed superior sealing ability compared to those prepared with ultrasonics.

Sealing ability of mineral trioxide aggregate and calcium-enriched mixture cement as apical barriers with different obturation techniques.

Endodontic treatment in pulpless immature teeth is challenging due to the lack of an apical stop. Insertion of an apical plug is an alternative to conventional long-term apexification with calcium hydroxide. The aim of this study was to compare the apical microleakage of mineral trioxide aggregate (MTA) and calcium-enriched mixture (CEM) cement as apical plugs with three different obturation techniques. This experimental study was conducted on 130 single rooted human teeth with one canal. Samples were randomly divided into 2 experimental groups (n=60) and two negative and positive control groups containing 5 samples each. After cleaning and shaping, an open apex configuration was prepared in all samples. MTA or CEM cement apical plugs with 5 mm thicknesses were placed. Then, each group was divided to 4 subgroups and the remaining space of root canals were filled with either lateral compaction or thermoplasticized injectable gutta-percha or was obturated by filling the entire canal with apical plug material. In one remaining subgroup the canal space was left unfilled. Microleakage was measured by the fluid filtration method and results were analyzed by means of the two-way ANOVA test. There were no significant differences between microleakage of MTA and CEM cement apical plugs (P=0.92). The difference between three obturation methods was not significant, either (P=0.39). MTA and CEM cement have similar sealing ability as apical plugs and no significant difference was found in microleakage of the three groups.

Effect of accelerants on the immediate and the delayed sealing ability of mineral trioxide aggregate when used as an apical plug: An in vitro study

Aim:To evaluate and compare the influence of various accelerators, 15% disodium hydrogen phosphate (Na2HPO4), 10% calcium chloride (CaCl2) and 23.1 wt% calcium lactate gluconate (CLG), on the immediate (after 72 h) and delayed (after 2 months) sealing ability of white ProRoot mineral trioxide aggregate (WMTA) when it is used as an apical plug.Materials and Methods:Eighty, single-rooted mandibular premolars were instrumented and standardized artificial open apices were created. The samples were then randomly assigned into four experimental groups and two control groups. WMTA was mixed with the respective accelerators and an apical plug of 4-mm thickness was fabricated. The remaining canal spaces were then backfilled. The samples were stored for the stipulated time periods and then immersed in 0.2% Rhodamine B solution for 72 h. Dye leakage was analyzed using a stereomicroscope.Results:Mean microleakage values of all experimental groups revealed that MTA + 23.1 wt% CLG showed the least leakage, followed by MTA + 15% Na2HPO4 and MTA + 10% CaCl2 with MTA + deionized water showing the maximum leakage at both the time intervals (P < 0.001). All the samples stored for a period of 2 months showed less leakage as compared with the samples stored for 72 h (P < 0.05).Conclusions:It was found that all three accelerators significantly accelerated the set of WMTA, of which 23.1 wt% CLG showed the best results, followed by 15% Na2HPO4 and 10% CaCl2. The sealing ability of all the experimental groups was significantly superior after 2 months as compared with that after 72 h.

Comparative evaluation of sealing ability of mineral tri-oxide aggregate with intact, removed and modified smear layer: An in vitro study

Objective: The aim was to evaluate the sealing ability of mineral tri-oxide aggregate (MTA) with intact, removed, and modified smear layer. 5.2% sodium hypochlorite was used for irrigation to keep smear layer intact, 17% ethylenediamine tetraacetic acid (EDTA) followed by 5.2% sodium hypochlorite was used to remove the smear layer and 30% potassium oxalate was used for its modification. Materials and Methods: A total of 112 intact single canaled mandibular premolar teeth were selected for the study. Roots were separated from the crown portion. All 112 roots were then randomly placed into one of five groups: Three experimental groups of 28 roots each, one group of 14 roots (negative control), and one group of 14 roots (positive control). All specimens were immersed in 1% methylene blue dye for 72 h and viewed microscopically. The results obtained were subjected to statistical analysis using one-way ANOVA test and unpaired Student's t-test. Results: When group 1a was compared with 2a and 3a, there was a statistically significant difference (P 0.05) when 1a and 3a were compared. When groups 1, 2, 3, 4 and 5 were compared irrespective of the subgroups, the results were statistically significant (P < 0.05) between 1 and 2, 1 and 4, 1 and 5, 2 and 3, 2 and 4, 2 and 5, 3 and 4, 3 and 5, and 4 and 5. Conclusion: About 5.2% sodium hypochlorite and MTA produced less leakage than 17% EDTA, followed by 5.2% sodium hypochlorite but more than 30% potassium oxalate.

In vitro comparison of induction capacity and biomineralization ability of mineral trioxide aggregate and a bioceramic root canal sealer

To compare the effect of mineral trioxide aggregate (MTA) and iRoot SP, a bioceramic root canal sealer, on the cell viability, hard tissue deposition capacity and odontogenic differentiation of human tooth germ stem cells (hTGSCs). The dental materials MTA, iRoot SP and Dycal were packed into Teflon rings and placed on transwell inserts for toxicity evaluations by the MTS assay on days 3 and 7. Dycal was used as a positive control for the cell viability assay. Teflon rings were cocultured with hTGSCs, followed by the induction of odontogenic differentiation. The odontogenic differentiation of hTGSCs and biomineralization ability of the materials were evaluated by analysing the mRNA expression levels of dentine sialophosphoprotein (DSPP) and collagen type 1A (COL1A) by real-time polymerase chain reaction expression analysis, measurement of alkaline phosphatase (ALP) activity and visualization of calcium deposits by von Kossa staining. MTA and iRoot SP exhibited no cytotoxicity, but Dycal caused cytotoxicity (P < 0.05) of almost all of the cells after 7 days. MTA significantly stimulated (P < 0.05) the odontogenic differentiation of hTGSCs compared with iRoot SP. MTA and iRoot SP increased (P < 0.05) the mRNA levels of COL1A and DSPP mRNA compared with noninduced hTGSCs, which served as a negative control (NC). iRoot SP, however, significantly decreased (P < 0.05) COL1A and DSPP mRNA expression levels compared with the PC. MTA and iRoot SP induced hTGSC differentiation into odontoblast-like cells, but MTA might provide more inductive potential and hard tissue deposition compared with iRoot SP.

Comparative evaluation of intracanal sealing ability of mineral trioxide aggregate and glass ionomer cement: An in vitro study

Aims:The purpose of this study was to compare the sealing ability of Mineral Trioxide Aggregate (MTA) and Glass Ionomer Cement (GIC) when used over gutta-percha as intracanal sealing materials. The study also evaluated the sealing ability of Zinc oxide eugenol (ZOE) cement and Acroseal sealer.Materials and Methods:Teeth were obturated with gutta-percha using sealer ZOE (group A, C, D) and Acroseal (group B). The groups were further divided into 2 subgroups (15 premolars each) on the basis of intracanal sealing material used: GIC subgroups (A1, B1) and MTA in subgroups (A2, B2). The clearing technique was used in this study for leakage evaluation. Seventy mandibular premolars were prepared using step-back technique and divided into experimental groups A and B (30 premolars each) and the positive and negative control groups C and D (5 premolars each).Statistical analysis used:Coronal microleakage was determined under stereomicroscope using 15X magnification. Data was statistically analyzed using one-way ANOVA followed by Post-Hoc Multiple comparison (Bonferroni).Results:MTA group leaked significantly less than GIC group (P < 0.05). Acroseal exhibited better sealing ability than ZOE sealer. Teeth with no intracanal barrier showed almost complete leakage.Conclusions:MTA may be preferred over GIC as an intracanal barrier.

Sealing ability of mineral trioxide aggregate, calcium phosphate and polymethylmethacrylate bone cements on root ends prepared using an Erbium: Yttriumaluminium garnet laser and ultrasonics evaluated by confocal laser scanning microscopy

Background:Surgical endodontic therapy comprises of exposure of the involved root apex, resection of the apical end of the root, preparation of a class I cavity, and insertion of a root end filling material. Mineral trioxide aggregate (MTA) is now the gold standard among all root end filling materials. MTA is however difficult to handle, expensive and has a very slow setting reaction.Aim:(1) To compare the sealing ability of MTA, polymethylmethacrylate (PMMA) bone cement and CHITRA Calcium phosphate cement (CPC) when used as root end filling material using Rhodamine B dye evaluated under a confocal laser scanning microscope. (2) To compare the seal of root ends prepared using an ultrasonic retroprep tip and an Er: YAG laser using three different root end filling materials.Statistical Analysis:Statistical analysis was performed using a one-way ANOVA and a two-way ANOVA, independent samples t-test and Scheffe's post hoc test using SPSS Version 16 for Windows.Results:All the three materials, namely MTA, PMMA BONE CEMENT and CHITRA CPC, showed microleakage. Comparison of microleakage showed maximum peak value of 0.86 mm for MTA, 0.24 mm for PMMA bone cement and 1.37 mm for CHITRA CPC. The amount of dye penetration was found to be lesser in root ends prepared using Er: YAG laser when compared with ultrasonics, but the difference was found to be not statistically significant.Conclusion:PMMA bone cement is a better material as root end filling material to prevent apical microleakage. MTA still continues to be a gold standard root end filling material showing minimum microleakage. Er: YAG laser is a better alternative to ultrasonics for root end preparations.