Ability Of Mineral Trioxide Aggregate Research Articles

The Effect of Various Water-soluble Chitosan Concentrations on Physical Properties and Antibiofilm Ability of Mineral Trioxide Aggregate

The Effect of Various Water-soluble Chitosan Concentrations on Physical Properties and Antibiofilm Ability of Mineral Trioxide Aggregate

Comparison of root end sealing ability of three retrograde filling materials in teeth with root apices resected at 900 using dye penetration method under fluorescent microscope

Background: The main factor contributing to surgical endodontic dissatisfaction is an inadequate seal at the apex. The material used for retrograde filling should prevent contaminants from penetrating periradicular tissue. So the objective of this study was to evaluate and compare the ability of Mineral Trioxide Aggregate (MTA), Zirconomer and bioactive bone cement to seal the root end as retrograde filling material with apices resected at 900 angles using dye penetration method under fluorescent microscope. Methods: 36 extracted teeth samples were cut at Cementoenamel junction horizontally. Following the customary protocols of, cleaning, shaping and obturation with gutta percha and AH Plus sealer, resection of samples was done 3mm at the apical end at 90 degree angle along the long axis of the tooth with the help of diamond disc. A depth of 3mm of root end cavity was made with diamond coated ultrasonic surgical tip. The samples were randomly allocated in 3 groups: Group I: MTA ; Group II : Zirconomer; Group III: Bioactive Bone Cement . Coating of nail varnish was done with the roots except at the tip. Each material was compacted in the retrocavity using small pluggers. All the retrofilled samples were kept in acridine orange for 24 hours, following which cleaning & bucco-lingual sectioning (vertical) was carried out. Confocal laser scanning fluorescent microscope was used for checking the dye penetration in these samples thereby evaluating sealing ability. Results: Group III (Bioactive Bone Cement) had the highest root end sealing ability with mean (1.4 ± 0.51) followed by Group I (MTA) (2.3 ± 0.65) and lastly Group II (Zirconomer) (4.1 ± 0.57) as root end filling material with apices resected at 900 angle using dye penetration method under fluorescent microscopy which were statistically significant. Conclusions: Bioactive Bone cement can be recommended as an acceptable retrograde filling material.

Fracture Resistance of Molars With Simulated Strip Perforation Repaired With Different Calcium Silicate-Based Cements.

Background and aims A root perforation is a connection between the root canal system and the external supportive tissues. Strip perforation (SP), occurring within root canals in a strip, can worsen the prognosis of a treated tooth, reduce its mechanical resistance, and impair the tooth structure. One of the suggested methods to treat SP is to seal it with a bio-material such as calcium silicate cement. Therefore, this in vitro study aimed to assess the molar structure impairment due to SP, which requires studying the fracture resistance, and the ability of mineral trioxide aggregate (MTA), bioceramic, and calcium-enriched mixture (CEM) to repair this perforation. Materials and methods Seventy-five molars were instrumented to size #25 and taper 4%, irrigated with sodium hypochlorite and ethylenediaminetetraacetic acid (EDTA), dried, and then divided randomly into five groups (G1-G5): in G1, root canals were filled with gutta-percha and sealer (negative control sample), whereas the rest of the groups (G2-G5) had a manual simulated SP made with Gates Glidden drill at the mesial root of the extracted molar, and filled with gutta-percha and sealer up to their perforation area; in G2, SP was filled with gutta-percha and sealer (positive control sample); G3 used MTA to repair the SP; G4 used bioceramic putty; and G5 used CEM. Fracture resistance tests of the molars were conducted in the crown-apical direction using a universal testing machine. One-way ANOVA test and Bonferroni test were used to study the significance of the differences in the mean values of the tooth fracture resistance, where statistical significance was set at 0.05. Results The ANOVA test showed that there were statistically significant differences between the fracture resistance (in newtons) values among groups (p = 0.000). The Bonferroni test showed that G2 had a smaller fracture resistance mean than the other four study groups (656.53 N; p = 0.000), and that of G5 was smaller than G1, G3, and G4 (794.40 N, 1083.73 N, 1025.20 N, and 1034.20 N, respectively; p = 0.000 in each pairwise comparison). Conclusion SP reduced the fracture resistance of endodontically treated molars. SP restored using MTA and bioceramic putty was better than that treated with CEM and similar to molars without SP. Moreover, MTA and bioceramic putty enhanced the fracture resistance of endodontically treated teeth to levels similar to molars without SP.

A scanning electron microscopic study evaluating the sealing ability of MTA, BiodentineTM, and new light-cure MTA used for furcal perforation repair.

To evaluate and compare the sealing ability of Mineral trioxide aggregate (MTA), BiodentineTM and light cure MTA used for the repair of furcal perforations using scanning electron microscope (SEM). The study sample comprised 45 extracted mandibular molars. The teeth were embedded in modeling wax. Standard access cavities were prepared in each tooth using a round bur and non-end cutting bur with a high-speed handpiece with water spray. Furcal perforations were made on the centre of the pulpal floor of each tooth using a 0.5mm round bur. The teeth were then randomly divided into 3 experimental groups of 15 specimens each based on the materials used to seal the perforation; Group A: MTA; Group B: BiodentineTM; Group C: Light cure MTA. All the sealed perforations were compacted with a moist cotton pellet, and the samples were stored in a closed container for 24 hours to allow the repair materials to set completely. After 24 hours the samples were sectioned longitudinally and the extent of marginal adaptation was measured using scanning electron microscopy. The sealing ability was evaluated by measuring the gap (in microns) between the pulpal floor and the material used for the furcal repair. The overall results showed that the marginal adaptation of light-cure MTA was better than both MTA as well as Biodentine. The mean space between the pulpal floor and the repair material was least for group C (2.29). Tuckey's post hoc test showed that a significant difference (p<0.05) existed between group C and group A & B. Light-cure MTA exbibits good sealing ability to dentin when compared to conventionally used Biodentine and Mineral Trioxide Aggregate. Key words:Calcium silicates, MTA, Biodentinte, New light-cure MTA, Perforation repair.

Sealing ability of mineral trioxide aggregate, Biodentine, and EndoSequence RRM putty used as retrograde restorative material: An in vitro bacterial leakage model study

Sealing ability of mineral trioxide aggregate, Biodentine, and EndoSequence RRM putty used as retrograde restorative material: An in vitro bacterial leakage model study

Comparison of sealing ability of mineral trioxide aggregate, biodentine with and without bioactive glass as furcation repair materials

Aim: The aim of this study was to evaluate the sealing ability of mineral trioxide aggregate (MTA), Biodentine with and without Bioactive glass (BG) as furcation repair materials by ultraviolet (UV) spectrophotometric analysis. Materials and Methods: Forty extracted human maxillary molars (n = 40) were selected and decoronated 3 mm above the cementoenamel junction and 3 mm below it and a defect in furcation was created. The samples were then divided into 4 groups of 10 (n = 10) each, Group I: MTA, Group II: Biodentine, Group III: BG + Biodentine and Group IV: BG + MTA and the defect was treated with respective furcation repair material. All the samples were then immersed in 2% methylene blue solution for 24 h and later stored in 65% nitric acid solution. The solution obtained was subjected to centrifuge at 3500 rpm for 5 min. From this solution, 100 μl of the supernatant was collected, analyzed in UV spectrophotometer at 550 nm with nitric acid as the blank and readings were recorded as absorbance units. Results: All four groups exhibited a significant difference in dye absorbance values (P &lt; 0.01). Group I, i.e., MTA showed the least dye absorbance values when compared with the other three groups. Data were analyzed using one-way analysis of variance and post hoc Tukey tests. The level of statistical significance was set at P &lt; 0.05. Conclusion: Within the limitations of the study, it can be concluded that MTA had superior sealing ability than Biodentine, whereas BG + Biodentine showed better sealing ability when compared with BG + MTA.

Comparative evaluation of mineral trioxide aggregate, endoseal, and biodentine in furcation perforation repair: A bacterial leakage study

Objectives: The aim of this study is a comparative evaluation of the sealing ability of mineral trioxide aggregate (MTA) Angelus, pozzolan-based Endoseal MTA, and Biodentine when used as sealing materials for furcation perforation. This was done with a bacterial leakage model. Materials and Method: Sixty-four permanent mandibular molars were selected and horizontally sectioned at middle third of the root. Cavities of 2 mm depth were prepared at the root ends. Access cavities were prepared, and the canal orifices and the root end cavities were restored with light cured resin. Perforations of diameter 1.6 mm were created in the center of the pulpal floor using a round bur in a low-speed handpiece. The teeth were randomly assigned to three experimental groups (n = 20). The perforation sites in Groups 1, 2, and 3 were repaired with MTA Angelus, EndoSeal MTA, and Biodentine, respectively. The teeth were inserted individually in an Eppendorf vial which was then placed in a McCartney's bottle containing nutrient broth. The reservoirs were filled with 0.5 ml of Enterococcus faecalis. The system was incubated at 37°C and checked for appearance of turbidity in the nutrient broth for 30 days, and these findings were noted. Level of significance was fixed at P = 0.05, and statistical analysis was done with Chi-square analysis using IBM SPSS statistics 20.0 (IBM Corporation, Armonk, NY, USA). Results: In the time interval of 30 days, all the materials showed contamination to varying degrees (MTA Angelus 8/20 samples, Endoseal MTA 10/20 samples, and Biodentine 7/20 samples). There was no significant difference between the three groups (P > 0.05). Conclusions: MTA Angelus, Endoseal MTA, and Biodentine showed contamination in a 30-day incubation period when used as furcation perforation seal materials.

Comparison of sealing ability of MTA and retroplast as root end filling materials evaluated under a stereomicroscope using rhodamine B dye: An In vitro study.

The aim of the study was to compare the sealing ability of Mineral Trioxide Aggregate (MTA) and Retroplast as root end filling materials using the Rhodamine B dye penetration method in vitro. Forty freshly extracted human single-rooted teeth were randomly divided into two groups of 20 teeth each, and standard root-end cavities were prepared in all teeth. The cavities were filled with MTA and Retroplast in groups 1 and 2, respectively. The specimens were then stored in 1% Rhodamine B dye for a week, following which the dye penetration with all samples was analysed under a stereomicroscope and scored according to Tronstad's criteria. The observations were compared with Chi square test at a 0.220 level of significance. 5% of the samples in both the groups showed no significant leakage. The leakage was deeper with the Retroplast group than with the MTA group; however, there was no statistically significant difference between the two groups. Both MTA and Retroplast are similar in their sealing potential as root-end cavity fillings with Retroplast reporting slightly deeper microleakage than MTA within the limits of the present study.

An in vitro evaluation of effect of internal matrices of hydroxyapatite and calcium sulfate on sealing ability of mineral trioxide aggregate and light cured glass Ionomer cement when used as furcation repair materials

An in vitro evaluation of effect of internal matrices of hydroxyapatite and calcium sulfate on sealing ability of mineral trioxide aggregate and light cured glass Ionomer cement when used as furcation repair materials

Sealing Ability of Mineral Trioxide Aggregate, Biodentine and Glass Ionomer as Root-End Materials: A Question of Choice.

The goal of this study was to determine the sealing ability of MTA, Biodentine, and Fuji IX as root-end materials after ultrasonic retrograde preparation using a dye penetration method. Seventy permanent anterior teeth were used in this study. Root canals were prepared using rotary endodontic files, and obturated using the lateral condensation technique. The resection of the root apices was performed perpendicularly to the long axis, and 3 mm deep retrocavities were made by ultrasonic tips. The samples were randomly divided into three experimental groups (N=20) and two control groups (N=5). The root-end cavities were filled with mineral-trioxide aggregate (Rootdent MTA, TehnoDent, Russia) in Group 1, Biodentine (Septodont, Saint Maur des Fossés, France) in Group 2 and Fuji IX GP (GC Corporation, Tokyo, Japan) in Group 3. The retrocavities of the positive control group were left unfilled, while retrocavities in the negative control group were sealed using a flowable composite material. The samples were made transparent, and dye penetration was assessed under a stereomicroscope (x10). The data were analyzed with the Kruskal-Wallis test and the Mann-Whitney U-test. The Biodentine group had a lower mean leakage value than the MTA and Fuji IX groups, and the difference was statistically significant (P<0.01). No statistical difference in apical microleakage was found between the MTA and Fuji IX groups (P>0.05). Within the limitations of this study, it was concluded that Biodentine provides better sealing ability as a root-end material than MTA and Fuji IX. There was no statistically significant difference between the sealing ability of MTA and Fuji IX as root-end materials.

Evaluation of Sealing Ability of Biodentine and Mineral Trioxide Aggregate in Permanent Molars using Dye Extraction Method-an Invitro Study

Objective: The aim of this study was to compare the sealing ability of MineralTrioxide Aggregate (MTA) and Biodentine when used to repair the furcal perforations in permanent molars using dye extraction method. Study design: Recently extracted permanent molars were collected and randomly sealed with MTA and biodentine, after preparing perforations in the furcation area by using a high-speed long shank round bur No:4. After setting of the material, specimens were kept in methylene blue dye for 24 hours to check the microleakage by the dye extraction method. Samples were then placed in vials containing 1ml of 65% nitric acid for three days for the extraction of the dye. The vials were centrifuged at 14000 rpm for 5 minutes to separate debris from the extracted dye. About 200 microlitres of the supernatant from each sample was then analyzed in a UV-visible spectrophotometer at 550nm wavelength using concentrated nitric acid as the blank, and readings were recorded as absorbance units.

Effect of diode laser irradiation and 10% citric acid conditioning on the sealing ability of mineral trioxide aggregate as a retrograde filling material

Aim: The aim was to evaluate the effect of diode laser irradiation and 5% citric acid conditioning on microleakage of mineral trioxide aggregate (MTA) when used as root-end filling material. Materials and Methods: Forty-five human anterior teeth were decoronated and subjected to root canal instrumentation. Three millimeters of apical root portion was resected, and root-end cavities were prepared using ultrasonic tips. The teeth were then randomly divided into three groups (n = 15), In Group 1 – root-end cavity was prepared and treated with citric acid followed by MTA application; in Group 2 – root-end cavity was prepared and treated with diode laser followed by MTA application; and in Group 3 – root-end cavity was prepared and treated with citric acid and laser application followed by MTA application. The specimens were then subjected to glucose leakage analysis at 48 h, 7 days, and 14 days. One-way ANOVA test was used for raw data and Chi-square test for consolidated data to test the significance of difference between the variables. SPSS software version 20 was used for analyzing the data. Results: Group 1 showed significantly more leakage than Group 2 and Group 3 at all time intervals, whereas Group 3 showed the least microleakage at 48 h, 7 days, and 14 days. Conclusion: MTA had a better sealing ability when root-end cavities were conditioned with 5% citric acid followed by diode laser irradiation.

Sealing ability of three different root repair materials for furcation perforation repair: An in vitro study.

Aim:The present study aimed to evaluate and compare the sealing ability of mineral trioxide aggregate (MTA)-Angelus, Biodentine™, and EndoSequence cement in furcation perforations using protein leakage assessment.Materials and Methods:The present study was conducted using seventy extracted human maxillary and mandibular molars with intact furcation. The samples were randomly allocated into three groups (n = 20) based on repair material used: Group 1 – MTA-Angelus, Group 2 – Biodentine™, and Group 3 – EndoSequence. Two additional groups served as positive and negative controls (n = 5). Using the leakage assessment apparatus, the presence of protein was detected with a reagent (Coomassive Brilliant Blue) every day for 60 days. One-way ANOVA and post hoc Tukey's test were used for statistical analysis using SPSS software.Results:All the groups exhibited protein leakage from day 1. Biodentine showed minimum (0.1201 mg/ml), MTA showed maximum (0.3738 mg/ml), and EndoSequence had intermediate (0.2465 mg/ml) leakage. None in the negative control and all of the positive control specimens leaked during the experimental period of 60 days.Conclusion:The newer biomaterials, Biodentine and Endosequence with better handling properties, could be used as alternatives to MTA-Angelus while repairing furcation perforations.

Evaluation of sealing abilitiy of mineral trioxide aggregate mixed with propylene glycol as a root canal sealer: A in vitro study

Sealing ability is one of the most important features of endodontic sealers. The main goal of the present study was to compare sealing ability of mineral trioxide aggregate (MTA)-propylene glycol (PG) with two commonly used resin-based and MTA-based sealers. In This in vitro study Seventy extracted single-root teeth were used. Canal preparation was carried out using hand and RaCe rotary files. Ten teeth were used as control. The root canals in positive and negative control groups were left empty. Remaining 60 teeth were randomly divided into following four groups (n = 15): In Group 1, the canals were dried using paper cones and obturated using MTA-PG sealer. In Group 2, saline was removed from canal using a syring, but paper cones were not used. Obturation was done using MTA-PG sealer. In Groups 3 and 4, the canals were dried using paper cones and obturated with AH26 and MTA Fillapex, respectively. Two-chamber method was used to evaluate bacterial leakage using Enterococcus faecalis (ATCC 29212). Turbidity of the lower chambers was checked every day during 90 days. Chi-square, Kaplan-Meier analysis, and logrank tests were used to compare groups regarding leaked samples at the end of the study. The level of significance was set at 0.05. There was no significant difference among groups regarding rate of leakage throughout the study. However, at the end of the study, the groups were statistically different regarding leaked samples (P = 0.034). MTA Fillapex and MTA-PG in dry canal showed the most and least leaked samples at the end of the study, respectively (P < 0.05). MTA mixed with PG has superior sealing ability than MTA Fillapex.

Healing of periapical lesion with mineral trioxide aggregate apexification

In the present study, the ability of mineral trioxide aggregate in the formation of apical plug for healing of large periapical lesion with open apex was assessed and evaluated the clinical outcome. Fifteen participants with periapical lesion at the upper anterior teeth with open apex were treated with mineral trioxide aggregate. The effect on healing of apical size was evaluated at 3, 6, and 12 months by radiological examinations in the form of periapical index criteria, diameter of the lesion size and the presence or absence of apical tissue barrier. The results found that neither pain nor any sinus was detected at 12 months. The mean size of the apical lesion was gradually reduced from 5.1 × 3.8 to 1.5 × 0.9 mm and mean PAI was reduced from 3.3 to 1.7 mm. The differences between mean size of periapical lesion at preoperative and 12 months observation period was also statistically significant (p&lt;0.05). The clinical success shown significant success rate of 93.3% analyzed with Z-test. In conclusion, tooth with open apex can be successfully treated with mineral trioxide aggregate apexification technique followed by root canal obturation.

Sealing ability of a retrograde filling materials using Amalgam, MTA, or Zinc Phosphate cement

This study evaluated the ability of mineral trioxide aggregate (MTA) to seal the root end effectively by comparing its apical sealing ability with that of amalgam and zinc phosphate cement. Forty five, single rooted extracted human teeth were used in this study, teeth divided randomly into three groups. After root–end resection of teeth, 3 mm depth of retrograde class I cavities were performed in 3 groups. Retrofillings of each group were performed with Amalgam, MTA and Zinc Phosphate cement. Following immersion in 1% Methylene blue dye for 42 hours, the roots were sectioned and the depth of dye penetration was evaluated by a stereomicroscope at X-10 magnification. The sealing ability of the retrograde filling materials was determined by their ability to inhibit dye penetration. Data were analyzed by one way ANOVA test and t-test. MTA provides a better seal than Amalgam and Zinc Phosphate cement when used as retrograde filling.

Biocompatibility and sealing ability of mineral trioxide aggregate and biodentine as root-end filling material: A systematic review.

Introduction:This systematic review intended to compare the biocompatibility and sealing ability of mineral trioxide aggregate (MTA) and biodentine as root-end filling material.Materials and Methods:A computerized literature search was performed on March 1, 2016, in MEDLINE, PubMed, and COCHRANE LIBRARY for data published from January 2011 to March 2016. Quality assessment of the selected studies was performed according to the PRISMA guidelines, 2009.Results:A total of 12 in vitro studies were included in this review. Of these, four studies compared the biocompatibility and eight compared the sealing ability. With regard to biocompatibility, two articles showed biodentine to be better and two showed comparable results, while in the case of sealing ability, one article showed MTA to be better, six articles showed biodentine to be better, and the rest one article showed the comparable result.Conclusion:It may be concluded that good sealing ability of biodentine along with its favorable biological properties show that materials can be used competently in clinical practice as a retrograde filling material. However, long-term assessment in clinical situations is necessary for further inferences.

The Effect of Intracanal Medicaments on Microleakage of Mineral Trioxide Aggregate Apical Plugs.

Introduction:The purpose of this in vitro study was to evaluate the effect of calcium hydroxide, double and triple antibiotic paste on the sealing ability of mineral trioxide aggregate (MTA) apical plugs. Methods and Materials:A total of 90 extracted teeth with single canals were prepared and randomly divided into four experimental groups (n=20). Intra-canal medicaments were applied for 3 weeks. MTA was placed through the access opening and condensed to the apical area and then fluid filtration technique was utilized to evaluate sealing ability after 1, 7, 14 and 30 days. Results:Triple antibiotic paste significantly reduced the sealing ability of MTA plug compared with double antibiotic paste (P=0.024) and normal saline (P=0.04) groups on day 1. The sealing ability was not different on days 14 and 30 between experimental groups (P>0.05). Conclusion:All medicaments can be used without any long term effect on microleakage.

Effect of Propylene Glycol on the Sealing Ability of Mineral Trioxide Aggregate and Calcium-Enriched Mixture Cement Apical Barriers.

Introduction:Propylene glycol (PG) improves the handling, physical, and chemical properties of mineral trioxide aggregate (MTA). This study aimed to evaluate the effect of PG on the sealing ability of MTA and calcium-enriched mixture (CEM) apical barriers. Methods and Materials: A total of 70 extracted human maxillary single-rooted teeth were prepared using ProTaper rotary system. The apical 3 mm of the root tips were resected and the root canals were enlarged with Peeso reamers up to #4, to create open apex teeth. The teeth were then randomly divided into four experimental (n=15) and two control (n=5) groups. Group1: MTA+ MTA liquid, group2; MTA+MTA liquid (80%) + PG (20%), group3; CEM+CEM liquid, group4; CEM+ liquid (80%) + PG (20%). Cements were mixed with their respective mixing agents and a 4-mm thick apical plug was fabricated. The microleakage was measured on day 1, 3, 7 and 21 using a fluid filtration technique. The repeated measures ANOVA and Sidak test were used to analyze the data. Results:All experimental groups demonstrated various amounts of microleakage. No significant difference was found between MTA and CEM cement (P=0.193), regardless of time and liquid components. There was no significant difference was observed between liquids (P=0.312) in all time intervals. The rate of microleakage decreased over time and a significant differences was observed between all intervals (P<0.05), except 3-7 and 7-21 (P=0.190) days. Conclusion:PG demonstrated neither a positive nor a negative effect on the sealing ability of Angelus MTA and CEM cement.

Solubility and bacterial sealing ability of MTA and root-end filling materials

ABSTRACTObjective To evaluate solubility and sealing ability of Mineral Trioxide Aggregate (MTA) and root-end filling materials.Material and Methods The materials evaluated were: MTA, Calcium Silicate Cement with zirconium oxide (CSC/ZrO2), and zinc oxide/eugenol (ZOE). Solubility test was performed according to ANSI/ADA. The difference between initial and final mass of the materials was analyzed after immersion in distilled water for 7 and 30 days. Retrograde cavities in human teeth with single straight root canal were performed by using ultrasonic tip CVD 9.5107-8. The cavities were filled with the evaluated materials to evaluate sealing ability using the bacterial leakage test with Enterococcus faecalis. Bacterial leakage was evaluated every 24 hours for six weeks observing the turbidity of Brain Heart infusion (BHI) medium in contact with root apex. Data were submitted to ANOVA followed by Tukey tests (solubility), and Kruskal-Wallis and Dunn tests (sealing ability) at a 5% significance level.Results For the 7-day period, ZOE presented highest solubility when compared with the other groups (p<0.05). For the 30-day period, no difference was observed among the materials. Lower bacterial leakage was observed for MTA and CSC/ZrO2, and both presented better results than ZOE (p<0.05).Conclusion MTA and CSC/ZrO2 presented better bacterial sealing capacity, which may be related to lower initial solubility observed for these materials in relation to ZOE.