Introduction: Prior injury is a risk factor for lower limb injury in female footballers. An improved understanding of the impact of the most common and severe injuries on lower limb strength and biomechanics may help to optimise rehabilitation strategies and decrease subsequent injury rates. The aim of this cross-sectional study was to compare lower limb strength and countermovement jump (CMJ) kinetics between elite female footballers with and without a history of anterior cruciate ligament reconstruction (ACLR), hamstring strain, or hip/groin injury. Methods: 369 elite female Australian football, soccer and rugby league players aged 15–35 years underwent assessments of isometric hip adductor and abductor strength, eccentric knee flexor strength, and CMJ vertical ground reaction forces. Players reported their lifetime history of ACLR, and whether they had sustained a hamstring strain, or hip/groin injury in the previous 12-months. Strength and jump/landing kinetics, including between-leg asymmetry, were compared between players with and without prior injury. Logistic regression was used to determine the variables that best distinguished between players with and without prior injury. Results: Players with a unilateral history of ACLR (n=24) displayed asymmetry (injured<uninjured leg) in eccentric knee flexor strength (mean difference=-6.9%, 95%CI=-9.1 to -4.8%), isometric hip abductor strength (mean difference=-2.4%, 95%CI=-4.4 to -0.4%), and peak landing force (mean difference=-5.9%, 95%CI=-11.0 to -0.9%). Together, between-leg asymmetry in eccentric knee flexor strength, isometric hip abductor strength, and CMJ peak landing force distinguished between players with and without prior ACLR with 93% accuracy. No differences in strength or CMJ kinetics were observed between players with and without a history of hamstring or hip/groin injury. Discussion: Elite female footballers with a history of ACLR, but not hamstring or hip/groin injury, exhibit persistent between-leg asymmetries in lower limb strength and jump kinetics following a return to sport. To address the effects of prior injury, ACLR rehabilitation programs for elite female footballers should consider targeting between-leg asymmetries in eccentric knee flexor strength, isometric hip abductor strength, and CMJ kinetics. Conflict of interest statement: Funding for travel costs associated with data collection was provided by Vald Performance (Brisbane, Australia) and SMA Research Grant. My co-authors and I acknowledge that we have no conflict of interest of relevance to the submission of this abstract.