Amino Derivatives Research Articles

Isohexide-Based Tunable Chiral Platforms as Amide- and Thiourea-Chiral Solvating Agents for the NMR Enantiodiscrimination of Derivatized Amino Acids.

New arylamide- and arylthiourea-based chiral solvating agents (CSAs) were synthesized starting from commercially available isomannide and isosorbide. The two natural isohexides were transformed into the three amino derivatives, having isomannide, isosorbide, and isoidide stereochemistry, then the amino groups were derivatized with 3,5-dimethoxybenzoyl chloride or 3,5-bis(trifluoromethyl)phenyl isothiocyanate to obtain the CSAs. Bis-thiourea derivative containing the 3,5-bis(trifluoromethyl)phenyl moiety with exo-exo stereochemistry was remarkably efficient in the differentiation of NMR signals (NH and acetyl) of enantiomers of N-acetyl (N-Ac) amino acids in the presence of 1,4-diazabicyclo[2,2,2]octane (DABCO). Nonequivalences in the ranges of 0.104-0.343 ppm and 0.042-0.107 ppm for NH and acetyl groups, respectively, allowed for very accurate enantiomeric excess determination, and a reliable correlation was found between the relative positions of signals of enantiomers and their absolute configuration. Therefore, a complete stereochemical characterization could be performed. Dipolar interactions detected in the ternary mixture CSA/N-Ac-valine/DABCO led to the identification of a different interaction model for the two enantiomers, involving the formation of a one-to-one substrate/CSA complex for (S)-N-Ac-valine and a one-to-two complex for (R)-N-Ac-valine, as suggested by the complexation stoichiometry.

Integration of widely targeted metabolomics and the e-tongue reveals the chemical variation and taste quality of Yunnan Arabica coffee prepared using different primary processing methods

UPLC-Q-TOF-MS and electronic tongue analysis were applied to analyse the metabolic profile and taste quality of Yunnan Arabica coffee under seven primary processing methods. The total phenolic content ranged from 34.44 to 44.42 mg/g DW, the e-tongue results revealed the strongest umami sensor response value in the sample prepared with traditional dry processing, while the samples prepared via honey processing II had the strongest astringency sensor response value. Metabolomics analysis identified 221 differential metabolites, with higher contents of amino acids and derivatives within dry processing II sample, and increased contents of lipids and phenolic acids in the honey processing III sample. The astringency and aftertaste-astringency of the coffee samples positively correlated with the trigonelline, 3,5-di-caffeoylquinic acid and 4-caffeoylquinic acid content. The results contributed to a better understanding of how the primary processing process affects coffee quality, and supply useful information for the enrichment of coffee biochemistry theory.

Antifungal properties of new 1,2,4-triazole derivatives (literature review)

In Ukraine, there is a targeted scientific and technical program for the development of technologies for the creation of modern domestic medicines. One of the most promising ways in this program is the development and implementation of drugs to combat various multi-resistant diseases. The study of the effect of biologically active substances on the structural components of mushrooms determines the possibility of finding new highly effective antifungal compounds. The aim of the work was the analysis and systematization of existing new data on the antifungal activity of a number of 1,2,4-triazole derivatives and the selection, based on the results of a comprehensive analysis of literary sources, of promising directions for further scientific research. Methods used: information search, synthetic, analytical, bibliographic, descriptive, generalization. The research materials were literary sources that contain information on the antifungal activity of a number of 1,2,4-triazole derivatives. The authors repeatedly claim that 1,2,4-triazole derivatives with a 1,2,3-benzotriazin-4-one fragment show significant antifungal potential against Candida albicans and Cryptococcus neoformans. Foreign researchers proved that a series of triazole-containing isoxazole compounds has antifungal activity against eight fungi pathogenic to humans. Another team of scientists proved the high antifungal activity of pyrimidine derivatives of 1,2,4-triazole. Domestic authors found that the modeling of 1,2,4-triazole due to the introduction of a Sulfur atom and subsequent transformations by this atom lead in many cases to the emergence of antifungal activity in molecules. Scientists of the Zaporizhzhya State Medical and Pharmaceutical University have proven the high antifungal activity of some amino derivatives of 1,2,4-triazole. The analysis of scientific publications dedicated to the study of antifungal properties of 1,2,4-triazole derivatives in recent years proves the indisputable prospect of finding new biologically active agents among substituted 1,2,4-thiazoles. Numerous publications indicate that in recent years, new molecules have appeared in a number of 1,2,4-triazole derivatives that have antifungal properties. The authors clearly and argumentatively proved the perspective of the direction of creating new original drugs for the treatment of various fungal pathologies.

Semisynthesis and characterization of versatile azide intermediates using sodium alginate and its homopolymeric derivatives as starting material

Alginate, a polyuronic biopolymer composed of mannuronic and guluronic acid units, contain hydroxyl and carboxyl groups as targeting modification sites to obtain structures with new and/or improved biological properties. The copper-catalyzed azide-alkyne cycloaddition (CuAAC) is a versatile click reaction for polymer functionalization, but it typically requires a “pre-click” modification to introduce azide or alkyne groups. Here, we described a straightforward chemical path to selectively modify alginate carboxyl groups producing versatile azido derivatives through N-acylation using 3-azydopropylamine. The resulting azide-functionalized polysaccharides underwent click chemistry to yield amino derivatives, confirmed by NMR and FTIR analyses. The 1H NMR spectrum reveals a characteristic triazole group signal at 8.15 ppm. The absence of the azide FTIR band for all amino derivatives, previously observed for the N-acylation products, indicated reaction success. Antibacterial and antioxidant assessments revealed that the initial polysaccharide lacks E. coli inhibition, while the click chemistry-derived amine products exhibit growth inhibition at 5.0 mg/mL. Lower molecular weight derivatives demonstrate superior DPPH scavenging ability, particularly amino-derivatives (24–33 % at 1.2 mg/mL). This innovative chemical pathway offers a promising strategy for developing polysaccharide structures with enhanced properties, demonstrating potential applications in various fields.

Integrated metabolome and transcriptome analyses reveal the molecular mechanism underlying dynamic metabolic processes during taproot development of Panax notoginseng

BackgroundPanax notoginseng (Burk) F. H. Chen is one of the most famous Chinese traditional medicinal plants. The taproot is the main organ producing triterpenoid saponins, and its development is directly linked to the quality and yield of the harvested P. notoginseng. However, the mechanisms underlying the dynamic metabolic changes occurring during taproot development of P. notoginseng are unknown.ResultsWe carried out metabolomic and transcriptomic analyses to investigate metabolites and gene expression during the development of P. notoginseng taproots. The differentially accumulated metabolites included amino acids and derivatives, nucleotides and derivatives, and lipids in 1-year-old taproots, flavonoids and terpenoids in 2- and 3-year-old taproots, and phenolic acids in 3-year-old taproots. The differentially expressed genes (DEGs) are related to phenylpropanoid biosynthesis, metabolic pathway and biosynthesis of secondary metabolites at all three developmental stages. Integrative analysis revealed that the phenylpropanoid biosynthesis pathway was involved in not only the development of but also metabolic changes in P. notoginseng taproots. Moreover, significant accumulation of triterpenoid saponins in 2- and 3-year-old taproots was highly correlated with the up-regulated expression of cytochrome P450s and uridine diphosphate-dependent glycosyltransferases genes. Additionally, a gene encoding RNase-like major storage protein was identified to play a dual role in the development of P. notoginseng taproots and their triterpenoid saponins synthesis.ConclusionsThese results elucidate the molecular mechanism underlying the accumulation of and change relationship between primary and secondary metabolites in P. notoginseng taproots, and provide a basis for the quality control and genetic improvement of P. notoginseng.

A Mini Review on the Chemical and Bio-Medicinal Aspects along with Energy Storage Applications of Anthraquinone and its Analogues

Abstract: Quinones have established enthusiasm of the researchers because of their enormous applicable properties. Researchers are now concerned about the synthesized and natural anthraquinone based derivatives due to their distinctive properties. Intensive research has been made with special focus on laxative, antiulcerogenic, inhibition of enzymes, cytotoxicity, antimicrobial, antiinflammatory, antiarthritic, antirheumatic, antidepressant, fungicidal, insecticidal, antioxidant and antitumor activity of anthraquinone based derivatives. The awareness of environmental protection, safety and health, free of pollution, disease and particularly to maintain green, are the derivatives being considered and acknowledged in medicinal research as well as in pharmaceutical world. Anthraquinone linked with amino derivatives has wide-spectrum therapeutic applications and a center of focus as an important biological scaffold in different fields like medicines, electronic industry, and cosmetics. Owing to the remarkable activities of anthraquinone, we outline the structural data, their sources, synthetic routes and therapeutic potentials.

Novel 2-amino-substituted (thio)morpholine-3,5-diones: synthesis and cytotoxicity studies

The present study explores the synthesis of new potential Cereblon ligands, 2-amino derivatives of (thio)morpholine-3,5-diones. The resulting compounds demonstrate notable cytotoxic effects in tests conducted in vitro on myeloma cell lines, highlighting the potential of these compounds as molecular glues.

Comparative transcriptome and metabolome profiles of the leaf and fruits of a Xianjinfeng litchi budding mutant and its mother plant.

Background: Litchi (Litchi chinensis) is an important sub-tropical fruit in the horticulture market in China. Breeding for improved fruit characteristics is needed for satisfying consumer demands. Budding is a sustainable method for its propagation. During our ongoing breeding program, we observed a litchi mutant with flat leaves and sharp fruit peel cracking in comparison to the curled leaves and blunt fruit peel cracking fruits of the mother plant. Methods: To understand the possible molecular pathways involved, we performed a combined metabolome and transcriptome analysis. Results: We identified 1,060 metabolites in litchi leaves and fruits, of which 106 and 101 were differentially accumulated between the leaves and fruits, respectively. The mutant leaves were richer in carbohydrates, nucleotides, and phenolic acids, while the mother plant was rich in most of the amino acids and derivatives, flavonoids, lipids and organic acids and derivatives, and vitamins. Contrastingly, mutant fruits had higher levels of amino acids and derivatives, carbohydrates and derivatives, and organic acids and derivatives. However, the mother plant's fruits contained higher levels of flavonoids, scopoletin, amines, some amino acids and derivatives, benzamidine, carbohydrates and derivatives, and some organic acids and derivatives. The number of differentially expressed genes was consistent with the metabolome profiles. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway-enriched gene expressions showed consistent profiles as of metabolome analysis. Conclusion: These results provide the groundwork for breeding litchi for fruit and leaf traits that are useful for its taste and yield.

Allosteric Activation of α7 Nicotinic Acetylcholine Receptors by Novel 2-Arylamino-thiazole-5-carboxylic Acid Amide Derivatives for the Improvement of Cognitive Deficits in Mice.

Enhancing α7 nAChR function serves as a therapeutic strategy for cognitive disorders. Here, we report the synthesis and evaluation of 2-arylamino-thiazole-5-carboxylic acid amide derivatives 6-9 that as positive allosteric modulators (PAMs) activate human α7 nAChR current expressed in Xenopus ooctyes. Among the 4-amino derivatives, a representative atypical type I PAM 6p exhibits potent activation of α7 current with an EC50 of 1.3 μM and the maximum activation effect on the current over 48-fold in the presence of acetylcholine (100 μM). The structure-activity relationship (SAR) analysis reveals that the 4-amino group is crucial for the allosteric activation of α7 currents by compound 6p as the substitution of 4-methyl group results in its conversion to compound 7b (EC50 = 2.1 μM; max effect: 58-fold) characterized as a typical type I PAM. Furthermore, both 6p and 7b are able to rescue auditory gating deficits in mouse schizophrenia-like model of acoustic startle prepulse inhibition.

In silico Design, Synthesis and Biological Evaluation of Novel Thieno[3,2-d]pyrimidine Derivatives for Cancer Therapy - A Preliminary Study on the Inhibitory Potential towards ATR Kinase Domain and PIKK Family.

Continuing our studies in the field of new heterocyclic compounds with biological interest, herein we report the synthesis and anticancer activity of new N- and S-substituted derivatives of tetracyclic pyrido[3',2' : 4,5]thieno[3,2-d]pyrimidines. In this regard, starting from the thieno[2,3-b]pyridine-2-carboxylates, the corresponding 8(9)-aminopyrido[3',2' : 4,5]thieno[3,2-d]pyrimidin-7(8)-ones, as well as chloro derivatives were obtained. Based on the latter, amino, hydrazino and S-alkyl derivatives of pyrido[3',2' : 4,5]thieno[3,2-d]pyrimidines were synthesized subsequently. The current study focuses on identifying the potential of thieno[3,2-d]pyrimidine derivatives primarily towards ATR kinase inhibition, through computational predictions, followed by synthesis and cancer cell viability studies, along with an aim to develop the core as PIKK inhibitors for cancer therapy.

Effects of bifenthrin pesticide on fermented cultures and metabolite profiles of kombucha tea

Pesticide residue contamination in raw materials influences the quality of fermented products. This study investigated the effects of bifenthrin (1, 3, 5, and 30 mg/L) in tea broth on the composition and metabolite profiles of kombucha tea. Bifenthrin at 30 mg/L (B30) showed an adverse effect on kombucha composition, with reduced 0.63–1 log CFU/mL of each microbial group (acetic acid bacteria, lactic acid bacteria, and yeast), while lower concentrations of bifenthrin gave decreased 0.30–0.84 log CFU/mL of each fermented culture. The presence of bifenthrin altered the kombucha metabolites and instigated intervening microbial metabolisms, such as arginine biosynthesis and pyrimidine metabolism, which resulted in reduced production of amino acids, amino derivatives, organic acids, and other beneficial compounds. B30 addition also reduced flavonoid content and antioxidant activity of kombucha tea by 16% (0.143–0.223 mg quercetin/mL) and 5% (0.239–0.278 mg Trolox/mL), respectively. Findings demonstrated that the presence of bifenthrin decreased kombucha tea quality in terms of chemical composition and reduced beneficial microbial populations by disrupting their biological pathways.

Synthesis and Biological Evaluation of Novel Amino and Amido Substituted Pentacyclic Benzimidazole Derivatives as Antiproliferative Agents.

Newly designed pentacyclic benzimidazole derivatives featuring amino or amido side chains were synthesized to assess their in vitro antiproliferative activity. Additionally, we investigated their direct interaction with nucleic acids, aiming to uncover potential mechanisms of biological action. These compounds were prepared using conventional organic synthesis methodologies alongside photochemical and microwave-assisted reactions. Upon synthesis, the newly derived compounds underwent in vitro testing for their antiproliferative effects on various human cancer cell lines. Notably, derivatives 6 and 9 exhibited significant antiproliferative activity within the submicromolar concentration range. The biological activity was strongly influenced by the N atom's position on the quinoline moiety and the position and nature of the side chain on the pentacyclic skeleton. Findings from fluorescence, circular dichroism spectroscopy, and thermal melting assays pointed toward a mixed binding mode-comprising intercalation and the binding of aggregated compounds along the polynucleotide backbone-of these pentacyclic benzimidazoles with DNA and RNA.

Fabrication of dual-functional Zr-based MOF incorporating amino and sulfoxide derivatives for simultaneous removal and detection of tetracycline antibiotics

Antibiotics contamination in aquatic environment has attracted considerable interest owing to its detrimental effects on human health and ecological safety. Therefore, developing more efficient materials for synchronous removal and detection of antibiotics is highly desired yet challenging. Herein, one novel zirconium-based metal–organic framework (Zr-MOF) was successfully constructed via a tetra-carboxylate ligand (H4SBTD-NH2) and demonstrated as a dual-functional platform for simultaneous removal and detection of tetracycline antibiotics. The ligand featuring amino and sulfoxide functionalization, facilitates the formation of variable pore sizes and a surface adorned with potential acting sites along the channels. Zr-MOF demonstrated high TCs adsorption capabilities (262.46 and 267.92 mg g−1 for TC and OTC, respectively). In addition to its high uptake, Zr-MOF exhibited exceptional sensitivity in the detection of TCs, particularly through the luminescence quenching effect, with remarkably nanomolar detection limits for TC (LOD 6.14 nM), and OTC (LOD 14.59 nM). These values are comparable to, or even exceed, the other excellent-performing MOF-based materials designed for removing and detecting of TCs. The outstanding performance of Zr-MOF should stem from a synergistic interplay of electron transfer, the inner filter effect (IFE), and specific host–guest interactions. The possible theoretical mechanism was investigated and discussed in detail by DFT calculations. This work provides a design basis for the development of materials that can both detect and remove specific pollutants from wastewater.

A Mini-Review on the Synthesis of Substituted Isatins: From Early Approaches to Recent Advances with a Specific Focus on Sulfamoyl and Amino Derivatives

Abstract: As a member of heterocyclic compounds, indole possesses a diverse set of biological activities. Studies on the structure-activity relationship of 2-indolinones have shown that they are important bioactive compounds in the field of pharmaceutical chemistry. Isatin and structures bearing isatin scaffold are derivatives of indole, which have shown to be of biological and pharmacological significance. This review focuses on different ways of obtaining compounds containing isatin structure, considering both earlier and recent methods of synthesis. The syntheses given herein were grouped into three categories, syntheses using anilines as their starting substance, synthetic methods based on the oxidation of indoles, and finally, due to the extensive research going on 5-substituted isatins and their possible pharmacological efficacy, pathways for synthesizing 5-amino and 5-sulfamoylisatins were gathered to underline the importance of 2-indolinone and isatin derivatives as prospective therapeutic compounds in current and future drug discovery studies.

A novel composition of endogenous metabolic modulators improves red blood cell properties in sickle cell disease.

The most common forms of sickle cell disease (SCD) are sickle cell anemia (SCA; HbSS) and HbSC disease. In both, especially the more dense, dehydrated and adherent red blood cells (RBCs) with reduced deformability are prone to hemolysis and sickling, and thereby vaso-occlusion. Based on plasma amino acid profiling in SCD, a composition of 10 amino acids and derivatives (RCitNacQCarLKHVS; Axcella Therapeutics, USA), referred to as endogenous metabolic modulators (EMMs), was designed to target RBC metabolism. The effects of ex vivo treatment with the EMM composition on different RBC properties were studied in SCD (n=9 SCA, n=5 HbSC disease). Dose-dependent improvements were observed in RBC hydration assessed by hemocytometry (MCV, MCHC, dense RBCs) and osmotic gradient ektacytometry (Ohyper). Median (interquartile range [IQR]) increase in Ohyper compared to vehicle was 4.9% (4.0%-5.5%), 7.5% (6.9%-9.4%), and 12.8% (11.5%-14.0%) with increasing 20×, 40×, and 80X concentrations, respectively (all p<0.0001). RBC deformability (EImax using oxygen gradient ektacytometry) increased by 8.1% (2.2%-12.1%; p=0.0012), 9.6% (2.9%-15.1%; p=0.0013), and 13.3% (5.7%-25.5%; p=0.0007), respectively. Besides, RBC adhesion to subendothelial laminin decreased by 43% (6%-68%; p=0.4324), 58% (48%-72%; p=0.0185), and 71% (49%-82%; p=0.0016), respectively. Together, these results provide a rationale for further studies with the EMM composition targeting multiple RBC properties in SCD.

Novel pyridine bearing pentose moiety-based anticancer agents: design, synthesis, radioiodination and bioassessments

Pyridine compounds are one of the most important heterocyclic derivatives showing wide ranges in biological and pharmacological activities. Green chemistry eliminates or reduces the generation of hazardous compounds. It prevents pollution at a molecular level. The microwave technique used in heterocyclic compound synthesis is also an important branch of green chemistry techniques. In this study, we report designing and synthesizing a new pyridine-bearing pentose moiety via a one-pot multicomponent reaction using D-glucose and also investigate its behavior and reactivity toward some simple and heterocyclic amino derivatives. The chemical structures of the synthesized compounds were characterized and tested for their cytotoxic activities. Some of the test compounds exhibited slight to high cytotoxic activities against Caco2 (colon cancer) cells, HepG2 (hepatocellular carcinoma) cells and MCF-7 (human breast cancer) cells by MTT assay. The results showed clearly that compound 4 and compound 8 displayed strongest to moderate cytotoxic activity against the HepG2, Caco2 and MCF-7 respectively and compound 1 showed good activity against MCF-7 in comparison to the standard anticancer drug doxorubicin. These data were by cytopathological examination. An in-vivo radioactive tracing study of compound 4 proved its targeting ability to sarcoma cells in a tumor-bearing mice model. Our findings suggest that the synthesized compounds may be promising candidates as novel anticancer agents.

Synthesis of N,N-spiro-bridged bis(cyclotriphosphazene) derivatives bearing aryl thio groups and their comparison with alkoxy and amino derivatives

In this study, bis (1a, b) and tetrakis (2a, b) cyclotriphosphazenes containing aryl thio groups were reacted with n-butyl amine. Deprotonation reactions of the obtained mono amino bis phenylthio (3a, b) and bis naphthylthio (4a, b) cyclotriphosphazenes were carried out in the presence of NaH base. New N,N-spiro bridged biscyclotriphosphazenes containing bis geminal phenylthio (5a-I and 5a-II) and naphthythio (5b-I and 5b-II) groups were created. Characterization of the newly synthesized compounds was performed by MALDI-TOF, 31P and 1H NMR spectroscopy, and single-crystal X-Ray crystallography (5a-I and 5b-I). Photophysical properties of compounds 1a, b, 2a, b, 5a-I, and 5b-I were determined using UV/vis and fluorescence spectroscopies. At the same time, the structures of N,N-spiro bridged biscyclophosphazenes were comprehensively evaluated with the structures of previously synthesized biscyclophosphazenes containing different functional groups.

Molecular Modeling Study of Bis-sulfonamide Derivatives Synthesis Targeting Aromatase Enzyme as Anticancer

Most studies indicate the important role of estrogen in the mechanism of occurrence and development of breast cancer. The importance of our research is the synthesis of bis-sulfonamide compounds that inhibit the aromatase enzyme, which is the main enzyme in the biosynthesis of estrogen. Molecular modeling of studied compounds was carried out by Molegro Virtual Docker (MVD) targeting aromatase enzyme and binding energy calculated to select the most encouraging compound. The highest binding energy among the studied compounds was -118.52 kcal/mol (compound A5) comparing with the aromatase substrate androstenedione -132.51 kcal/mol and the aromatase inhibitor letrozole -136.52 kcal/mol. Several of these compounds were synthesized in a simple way with good yields by reacting sulfonyl chloride derivatives with amino derivatives in an alkaline aqueous solution, or in a pyridine solution. The physicochemical characteristics and identification of synthesized compounds were determined by various analytical methods such as Mass spectrometry, Infrared spectroscopy and Nuclear Magnetic Resonance.

Removal of organic amino derivatives from wastewater by using fluorescent nitrogen-doped GQDs under visible light and fluorescence application under ultra violet light

Photocatalysis is one of the most popular organic waste degradation treatments because it can result in the total decomposition of organic pollutants under visible light illumination. Here, we present a metal-free visible-light active catalyst for the effective decomposition of organic aromatic amino derivatives Methylene blue (MB) and Rhodamine B (RB) as model-contaminated dye molecules. Through a one-pot hydrothermal process, blue colour emissive nitrogen-doped graphene quantum dots (N-GQDs) were synthesized from urea and malic acid. The physicochemical characteristic techniques were used to analyze the surface morphology, elemental analysis, surface functional groups, absorption, emission, and band gap of prepared N-GQDs. The effective light absorption properties, transfer of electrons, separation of photoinduced charge carriers, and excellent stability with a band gap of 2.69 eV of synthesized N-GQDs have enabled the degradation of Rh B and MB up to 90 % and 86 % respectively, within 120 min of visible light irradiation. Based on extensive active species trapping experiments, a probable photocatalytic dye degradation mechanism is proposed. These N-GQDs can also be used to make fluorescent ink for imaging and security purposes.

Effects of biochar pyrolysis temperature on uranium immobilization in soil remediation: Revealed by 16S rDNA and metabolomic analyses

Uranium-stressed soil caused by nuclear industry development and energy acquisition have attracted extensive attentions for a long time. This study investigated the effects of biochar application with different pyrolysis temperatures (300 ℃, 500 ℃ and 700 ℃) on remediation of uranium-stressed soil. The results showed that higher pyrolysis temperature (700 ℃) was benefit for ryegrass growing and caused a lower uranium accumulation in plants. At the same time, uranium immobilization was more effective at higher biochar pyrolysis temperature. Careful investigations indicated that activities of soil urease and sucrase were promoted, and bacterial diversity was strengthened in C700 group, which may contribute to uranium immobilization. The biochar application could activate metabolic of lipids and amino acids, organic acids and derivatives, and organic oxygen compounds. Nicotinate and nicotinamide metabolism, and Benzoxazinoid biosynthesis were unique metabolic pathways in the C700 group, which could enhance the uranium tolerance from different perspectives. Based on these results, we recommend to use biochar with 700 °C pyrolysis temperature when processing remediation of uranium-stressed soil. This study will facilitate the implementation of biochar screening and provide theoretical helps for remediation of uranium-stressed soil.