15N Recovery Research Articles

Plant and microbial uptake of nitrogen and phosphorus affected by drought using 15N and 32P tracers

Competition for nutrients between plants and microbes is an important determinant for plant growth, biodiversity and carbon cycling. Perturbations such as drought affect the availability of nitrogen (N) and phosphorus (P), and may cause shifts in uptake of N and P between plants and microbes. Competitiveness for these nutrients may depend on how flexible plants and microbes are in taking up N and P. We used a novel dual isotope labelling technique (15N and 32P) to assess short-term uptake of N and P by plants and microbes affected by drought in two different plant–soil systems. Mesocosms were extracted from two grassland sites differing in soil nutrient availability and plant species. Half of the mesocosms were subjected to drought one week prior to injection of 15N (as KNO3) and 32P (as H3PO4) tracers. Uptake rates of NO3− and P in plants and microbes were estimated based on average source pool enrichment during the labelling period and on plant and microbial recovery of 15N and 32P measured after 4 days of labelling. Overall competition for N and P was reduced with drought as less NO3− and P was taken up in plants and microbes. However, plant uptake of NO3− was more sensitive to drought than microbial NO3− uptake, while microbial P uptake was more sensitive than plant P uptake. These different sensitivities to drought by plants and microbes may decouple the N and P cycle with increased drought conditions.

Phosphorus addition enhances loss of nitrogen in a phosphorus-poor soil

Plants and microbes have limited stoichiometric flexibility to take up and store nitrogen (N) and phosphorus (P). Variation in the relative availability of N and P to plants and microbes may therefore affect how strongly N and P are held in terrestrial ecosystems with important implications for net primary productivity and carbon sequestration. We hypothesized that an increase in P availability in a P-poor soil would increase N uptake by plants and microbes thereby reducing N loss. We grew mixtures of the C3 grass Phalaris aquatica L. and the legume Medicago sativa L. in mesocosms with soils low in P availability and then used a novel technique by adding a 15N tracer with and without 1 g P m−2 to soil with different moisture and available N conditions, and measured the 15N recovery after 48 h in microbes, plants and soil. In contrast to our hypothesis, we found that P addition reduced 15N in microbes without water stress by 80% and also reduced total15N recovery, particularly without water stress. Water stress in combination with N addition further showed low total 15N recovery, possibly because of reduced plant uptake thereby leaving more 15N in the soil available for nitrification and denitrification. Our results suggest that P addition can result in large gaseous N loss in P-poor soils, most likely by directly stimulating nitrification and denitrification.

Effect of Addition of New Crop Residues on Recovery of 15n Previously Added Residues by Maize

Effect of Addition of New Crop Residues on Recovery of 15n Previously Added Residues by Maize

Characteristics of Nitrogen Uptake, Use and Transfer in a Wheat-Maize-Soybean Relay Intercropping System

Intercropping and relay intercropping systems, which significantly improve land use efficiency, are used worldwide to increase crops yield. The wheat-maize-soybean relay intercropping system has been widely employed by famers in Southwestern China for years, but the detailed mechanisms through which the nitrogen fertilizer use efficiency reach the high level in this system remain unclear. In the present study, two separate pot experiments were performed by 15 N isotope dilution (ID) labeling and direct 15N foliar feeding (FF) assays, and a solid barrier was employed to prevent the roots intergrowth and N movement among crops in the first experiment, using no barrier as the control. The results showed that, under the no-barrier condition, the grain yields, 15N uptake and 15N recovery efficiency of wheat and maize were significantly increased, but those measures in soybean were decreased compared to the solid barrier condition. Furthermore, bi-directional N transfer was detected during the co-growing stage of crops, the amount (Ntransfer) and percentage (%NT) of 15N transferred varied significantly with the fertilizer-N rate, and the maximum reached at 150 – 300 kg N ha–1 level. The Ntransfer from maize to wheat was 16.1% – 163.0% higher than that from wheat to maize; the Ntransfer from soybean to maize was 1.7 – 6.0 times higher than those from maize to soybean, while the %NT from soybean to maize were 6.7 – 22.2 times higher than those from maize to soybean. Conclusively, this study revealed that the interaction of the roots among crops significantly increased the uptake efficiency and recovery efficiency, and further, the positive N competition and bi-directional N transfer of each crops were the main contributors to improve the N use efficiency in the wheat-maize-soybean relay intercropping system.

Earthworms Reduce Biotic 15-Nitrogen Retention in Northern Hardwood Forests

Invasive exotic earthworms are significantly influencing understory community composition, soil, and ecosystem processes in northern hardwood forests in North America, but their effect on the retention of nitrogen (N) has been inconclusive. We examined this in two northern hardwood forest sites in New York state, USA through a tracer study. In both spring and fall, we added tracer amounts of 15N as nitrate—to simulate atmospheric deposition—with the biologically less active tracer bromide (Br−) to areas both with and without large populations of invasive earthworms. Total recovery of 15N was lower in earthworm-invaded plots, largely due to less retention in litter and upper soil horizons. Although the strong relationship between retention in the upper soil horizons and total 15N recovery suggests that earthworm destruction of the forest floor may be one mechanism reducing the capacity for N retention, in some cases the mineral soil in earthworm-invaded plots retained substantial N. Biotic pools, particularly litter and microbial biomass, retained significantly less 15N in earthworm-invaded plots than in their uninvaded counterparts. In plots invaded by earthworms, negative effects of earthworms on trees were revealed through root-uptake assays suggesting somewhat greater plant demand for ammonium in the spring and in lower 15N recovery in maple seedlings the year following tracer addition. Although similar patterns of Br− movement across treatments suggested that earthworms had smaller effects on hydrologic tracer movement than expected, they appear to have significant effects on the biological processes that underlie N retention.

Assessing agronomic and environmental implications of different N fertilisation strategies in subtropical grain cropping systems on Oxisols

A multi-season 15N tracer recovery experiment was conducted on an Oxisol cropped with wheat, maize and sorghum to compare crop N recoveries of different fertilisation strategies and determine the main pathways of N losses that limit N recovery in these agroecosystems. In the wheat and maize seasons, 15N-labelled fertiliser was applied as conventional urea (CONV) and urea coated with a nitrification inhibitor (DMPP). In sorghum, the fate of 15N-labelled urea was monitored in this crop following a legume ley pasture (L70) or a grass ley pasture (G100). The fertiliser N applied to sorghum in the legume-cereal rotation was reduced (70 kg N ha−1) compared to the grass-cereal (100 kg N ha−1) to assess the availability of the N residual from the legume ley pasture. Average crop N recoveries were 73 % (CONV) and 77 % (DMPP) in wheat and 50 % (CONV) and 51 % (DMPP) in maize, while in sorghum were 71 % (L70) and 53 % (G100). Data gathered in this study indicate that the intrinsic physical and chemical conditions of Oxisols can be extremely effective in limiting N losses via deep leaching or denitrification. Elevated crop 15N recoveries can be therefore obtained in subtropical Oxisols using conventional urea while in these agroecosystems DMPP urea has no significant scope to increase fertiliser N recovery in the crop. Overall, introducing a legume phase to limit the fertiliser N requirements of the following cereal crop proved to be the most effective strategy to reduce N losses and increase fertiliser N recovery.

Recovery of N applied as 15N-manure or 15N-gliricidia biomass by maize, cotton and cowpea

Proper management of N applied in fertilizers is important to optimize crop production and to avoid negative environmental impacts. The best way to study N dynamics in the soil plant system is to use fertilizers labeled with 15N. Recoveries of nitrogen following fertilization with 15N-labeled goat (Capra hircus L.) manure and gliricidia (Gliricidia sepium Jacq. Walp) biomass were evaluated in a greenhouse experiment with three successive planting cycles of three crops: maize (Zea mays L.), cotton (Gossypium hirsutum L.), and cowpea (Vigna unguiculata (L.) Walp.). Each 1 kg soil pot received 8 g (equivalent to 20 Mg ha−1) of either manure (12.3 mg g−1 of N) or gliricidia (37.8 mg g−1 of N). Plants were harvested 50 days after germination and real (15N) and apparent recoveries of the applied N were determined. Biomass and N amounts in the cotton and maize crops in all three cycles were higher with gliricidia application than with manure, except for cotton in the first cycle. The biomass of cowpea was also higher with gliricidia in the first and second cycles but the amount of N was significantly higher only in the second cycle. In the first cycle, the largest recoveries of 15N were obtained with gliricidia, for all three crops, but in the second and third cycles recoveries were greater with manure, so that the real recoveries of gliricidia and manure were similar (cotton, 35 and 37 %; maize, 27 and 26 %; and cowpea, 41 and 38 % of the applied N, respectively). Estimates of apparent recoveries were different from the real ones and therefore inadequate for cotton and cowpea. The fast release of N from gliricidia prunings and, on the other hand, the strong residual effect of goat manure-N to subsequent cropping cycles should be considered by farmers in their fertilization strategies.

Nutrient partitioning of Merino sheep divergently selected for genetic difference in resistance to Haemonchus contortus

This research was designed to determine if divergent genetic selection for resistance to Haemonchus contortus had produced correlated changes in the metabolism of amino-nitrogen in the absence or presence of H. contortus infection. Partitioning of amino acid-nitrogen between tissues was determined in 42 Merino weaner wethers from the CSIRO Haemonchus selection flock, increased resistance to Haemonchus (IRH), decreased resistance to Haemonchus (DRH) and random bred control (C) selection lines. Weaner wethers were fed a restricted diet (9.8MJME/kgDM, 86gMP/kgDM) calculated to allow a gain of 125g/d bodyweight throughout the experimental period and were either worm-free or trickle infected with H. contortus. At 8 weeks post-infection animals were injected with 15N-labelled duckweed directly into the abomasums. Animals were euthanased at either 6 or 24h after the injection to collect tissue samples for calculation of percentage recovery of 15N in tissue and to determine abomasal worm counts. Worm egg count and worm counts at week 8 of infection were lower in animals from the IRH line. IRH animals had a lower N digestibility, increased oxidation of amino acids and lower N balance but whole-body protein flux was unaffected. Amino acid metabolism, as assessed from 15N uptake and excretion in response to H. contortus infection, differed between IRH and DRH animals. In IRH animals a greater recovery of 15N in the thymus and abomasal smooth muscle indicated greater partitioning of amino acids towards the immune response. In DRH animals an increased recovery of 15N in the spleen, in response to infection, may be a possible adaptation. It appears that divergent selection for worm egg count has not been associated with symmetrical changes in amino acid metabolism, but rather the partitioning of amino acid resources reflects each selection line's independent response to infection.

Tree–grass interactions for N in Nothofagus antarctica silvopastoral systems: evidence of facilitation from trees to underneath grasses

Nothofagus antarctica forests in south Patagonia are usually used as silvopastoral systems but how grasses and trees compete for specific resources, such as nitrogen in these systems is unknown. To understand interactions between grasses and N. antarctica trees for N, an experiment with 15N labeled fertilizer was carried out comparing N absorption by grasses growing under trees (silvopastoral system) with an open site. Labeled 15NH 154 NO3 fertilizer at 10 % atom excess was added in spring at both sites and 15N was measured in herbage, soil and trees every 30 days during the growing season. Soil was the component that containing the greatest amount of N and greatest 15N recovery. Grasses growing in the silvopastoral system absorbed almost double of the fertilizer applied than grasses in the open site (32.4 kg N ha−1derived from fertilizer based on 15N recovery). Roots were also an important fate for N absorbed, representing 50 and 63 % of total 15N recovered in grass roots of open and silvopastoral sites, respectively. Trees absorbed 69 % less applied N than grasses in the silvopastoral system; being mainly allocated in small branches, sapwood and fine roots. Overall, 15N recovery was 65 % higher in the silvopastoral system (tree + grasses) than in the open site (grasses). Silvopastoral system made more efficient use of the 15N added. These results indicated that N. antarctica trees in the silvopastoral system may “facilitate” fertilizer N absorption of grasses by improving environmental conditions like water availability or by reducing competition for inorganic N between soil microorganisms and plants.

In-situ root uptake and soil transformations of glycine, glutamine and ammonium in two temperate deciduous forests of contrasting N availability

Whereas it is recognized that most trees have the capacity to take up free amino acids, it is unclear how important amino acid uptake is in situ. In-situ root uptake of isotopically-labeled ammonium, glycine and glutamine were compared between low-nitrogen (N)-availability and high-N-availability temperate forests. Comparable immediate 13C- and 15N-enrichment of fine roots indicated that short-term uptake of amino-acids by tree roots was similar between the two sites. Over the remainder of the experiment, recovery of amino-acid 15N in fine roots was greater, and recovery of 13C lower, in the high-N-availability site. Together with greater rates of conversion of amino-acid-derived 15N into inorganic forms, this indicates that a greater proportion of amino-acid-derived N was taken up after it was converted to inorganic forms by soil microorganisms in the high N availability site. Results from this study suggest that capacity for amino acid uptake by tree roots did not differ between high- and low-N availability sites, but differences in microbial mineralization drove strong differences in the availability of inorganic forms of N between sites.

Productivity, 15N dynamics and water use efficiency in low‐ and high‐input switchgrass systems

AbstractSustainable and environmentally benign switchgrass production systems need to be developed for switchgrass to become a large‐scale dedicated energy crop. An experiment was conducted in California from 2009 to 2011 to determine the sustainability of low‐ and high‐input irrigated switchgrass systems as a function of yield, irrigation requirement, crop N removal, N translocation from aboveground (AG) to belowground (BG) biomass during senescence, and fertilizer 15N recovery (FNR) in the AG and BG biomass (0–300 cm), and soil (0–300 cm). The low‐input system consisted of a single‐harvest (mid‐fall) irrigated until flowering (early summer), while the high‐input system consisted of a two‐harvest system (early summer and mid‐fall) irrigated throughout the growing season. Three N fertilization rates (0, 100, and 200 kg N ha−1 yr−1) were applied as subtreatments in a single application in the spring of each year. A single pulse of 15N enriched fertilizer was applied in the first year of the study to micro‐plots within the 100 kg N ha−1 subplots. Average yields across years under optimal N rates (100 and 200 kg ha−1 yr−1 for low‐ and high‐input systems, respectively) were 20.7 and 24.8 Mg ha−1. However, the low input (372 ha mm) required 47% less irrigation than the high‐input system (705 ha mm) and achieved higher irrigation use efficiency. In addition, the low‐input system had 46% lower crop N removal, 53% higher N stored in BG biomass, and a positive N balance, presumably due to 49% of 15N translocation from AG to BG biomass during senescence. Furthermore, at the end of 3 years, the low‐input system had lower fertilizer 15N removed by harvest (26%) and higher FNR remaining in the system in BG biomass plus soil (31%) than the high‐input system (45% and 21%, respectively). Based on these findings, low‐input systems are more sustainable than high‐input systems in irrigated Mediterranean climates.

Partitioning of N-uptake from 1-year of fertigation with 15N-urea in pot-lysimeter-grown M.9-grafted apple trees after 3 years of fertigation with unlabelled urea at three rates of N

SummaryThe total amounts of N derived from 15N-urea in the organs of 18 newly-bearing “maiden” apple trees and in the soil were determined after 1-year of fertigation with 15N-urea by drip irrigation, scheduled at a soil matric potential of –50 kPa. Prior to this treatment, the 18 “maiden” apple trees had been fertigated with three concentrations of N for 3 years: 17 mg N l–1 (low), 34 mg N l–1 (medium), or 67 mg N l–1 (high), and the same rates of 15N were chosen for treatment comparisons over the following 1 year. The trees were fertiligated with an aqueous stock solution of 15N-labelled urea (1.5 atom % 15N), KH2PO4, and KCl by drip irrigation. The phosphorus (P) and potassium (K) concentrations were constant, and set at 17 mg l–1 and 34 mg l–1, respectively. The total irrigation volume applied per tree was 363 l for the low, 430 l for the medium, and 216 l for the high N treatment.The corresponding supplies of N per tree were 6.17, 14.62, and 14.47 g, respectively. The total amounts of urea-N absorbed per tree were 1.90 g for the low-N, 3.73 g for the medium-N, and 3.21 g for the high-N treatments, with the percentage of N recovery (30.8%, 25.5%, and 22.1%) decreasing with increasing N rate, while the N retained in the soil was 2.92 g (47.3%), 7.40 g (50.6%), and 8.35 g (55.7%), respectively. Fertiliser N contributed more to whole-tree N uptake at increasing rates of N than N uptake from the soil. The total recovery of 15N from each tree-soil system was 78.1% at the low, 76.1% at the medium, and 79.8% at the high N level, with an average of approx. 22.0% of N unaccounted-for. The total amounts of P and K taken up were 2.42 g tree–1 and 14.49 g tree–1 for the low-N, 2.47 g tree–1 and 15.33 g tree–1 for the medium-N, and 1.31 g tree–1 and 8.04 g tree–1 for the high-N treatments, respectively. Our results showed that an N concentration of 34 mg l–1 (medium) resulted in more productive and profitable trees, bearing more fruit of higher quality than the other two N treatments. This concentration of N also improved the development of new leaves and flowers during the early stages of growth in the following Spring.

A 15N-Labeling Study of the Capture of Deep Soil Nitrate from Different Plant Systems

The objective of this study was to determine the efficiency of different plant systems in capturing deep soil nitrate (NO3−) to reduce NO3− leaching in a field plot experiment using 15N labelling. The study was conducted on a calcareous alluvial soil on the North China Plains and the plant systems evaluated included alfalfa (Medicago sativa), American black poplar (Populus nigra) and cocksfoot (Dactylis).15N-labelled N fertilizer was injected to 90 cm depth to determine the recovery of 15N by the plants. With conventional water and nutrient management, the total recovery of 15N-labeled NO3−-N was 23.4% by alfalfa after two consecutive growth years. The recovery was significantly higher than those by American black poplar (12.3%) and cocksfoot (11.4%). The highest proportion of soil residual 15N from the labeled fertilizer N (%Ndff) was detected around 90 cm soil depth at the time of the 1st year harvest and at 110-130 cm soil depth at time of the 2nd year harvest. Soil %Ndff in 0-80 cm depth was significantly higher in the alfalfa treatment than those in all the other treatments. The soil %Ndff below 100 cm depth was much lower in the alfalfa than those in all the other treatments. These results indicated that 15N leaching losses in the alfalfa treatment were significantly lower than by those in the black poplar and cocksfoot treatments, due to the higher root density located in nitrate labeling zone of soil profile. In conclusion, alfalfa may be used as a plant to capture deep soil NO3− left from previous crops to reduce NO3− leaching in high intensity crop cultivation systems of North China Plain.

Differentiating between the supply of N to wheat from above and belowground residues of preceding crops of pea and canola

Belowground N comprises an important component of total residue N, but the N from this pool is often not included in studies that use 15N approaches to trace residue-derived N in succeeding crops. The objective of this greenhouse study was to differentiate between N supplied from aboveground and belowground crop residues, including rhizodeposits, of pea and canola to wheat using 15N labeling. A cross 15N-labeling approach was used in which wheat was grown on 15N-labeled belowground residues and non-labeled aboveground residues and vice versa. On average, the amount of N in wheat derived from belowground residues was almost twice the amount from aboveground residues. The higher input of both aboveground and belowground residue biomass and hence residue N from canola compared to pea tended to increase the residue-derived N in wheat from canola (6.4 %) relative to pea (4.7 %). However, differences in the percent recovery of 15N based on the amount of residue-15N initially applied revealed that a higher proportion of belowground residue N from pea (13.4 %) was recovered in wheat compared to the corresponding aboveground residue N from pea (8.8 %), and both belowground and aboveground residue N from canola (6.5 and 7.3 %, respectively). The total supply of N to wheat from preceding pea and canola crops was relatively low, likely due to wide C/N ratios. This study demonstrates the importance of belowground residue N to the supply of N to succeeding crops as well as differences between species.

Mixed afforestation of young subtropical trees promotes nitrogen acquisition and retention

SummaryAfforestation is globally increasing to produce timber and pulp wood, but also to enhance ecosystem services such as carbon sequestration, nutrient retention or groundwater recharge. In China, large areas have been and will be afforested in order to compensate for the negative impacts of former clear‐cuttings and to make use of the ecosystem services associated with afforestation. In order to further optimize these services with regard to balanced nutrient (particularly nitrogen) cycles, it is important to know whether the use of mixtures of native tree species in afforestation projects promotes the acquisition and retention of nitrogen compared with the currently established large‐scale monocultures.To test the effect of species richness on system N retention and tree sapling N uptake, we conducted a15N tracer experiment in a young tree plantation. To this end, saplings of four abundant early successional tree species were planted in monocultures, in two‐ and four‐species mixtures and as single trees.Nitrogen retention increased with higher species richness due to enhanced N pools in sapling biomass. These species richness effects strengthened over time.Species‐specific differences in15N recoveries over time revealed below‐ground niche differentiation with regard to N uptake, which is likely to result in complementary resource use among coexisting species.Synthesis and applications. This study provides evidence that mixed afforestation promotes N retention from the sapling stage. To further improve ecosystem services associated with afforestation, we strongly suggest the use of mixtures of native tree species instead of monocultures. Mixtures of four species may reduce system N losses and thus may lessen groundwater contamination due to N leaching. We encourage further investigations to find optimal species combinations that promote a wide range of ecosystem services related to more closed nutrient cycles and minimized soil erosion. In our study, the plantations' capability to retain N could be optimized by means of both increasing tree species richness and by choosing the optimal species combinations.

Earthworm effects on the incorporation of litter C and N into soil organic matter in a sugar maple forest

To examine the mechanisms of earthworm effects on forest soil C and N, we double-labeled leaf litter with 13C and 15N, applied it to sugar maple forest plots with and without earthworms, and traced isotopes into soil pools. The experimental design included forest plots with different earthworm community composition (dominated by Lumbricus terrestris or L. rubellus). Soil carbon pools were 37% lower in earthworm-invaded plots largely because of the elimination of the forest floor horizons, and mineral soil C:N was lower in earthworm plots despite the mixing of high C:N organic matter into soil by earthworms. Litter disappearance over the first winter-spring was highest in the L. terrestris (T) plots, but during the warm season, rapid loss of litter was observed in both L. rubellus (R) and T plots. After two years, 22.0% +/- 5.4% of 13C released from litter was recovered in soil with no significant differences among plots. Total recovery of added 13C (decaying litter plus soil) was much higher in no-worm (NW) plots (61-68%) than in R and T plots (20-29%) as much of the litter remained in the former whereas it had disappeared in the latter. Much higher percentage recovery of 15N than 13C was observed, with significantly lower values for T than R and NW plots. Higher overwinter earthworm activity in T plots contributed to lower soil N recovery. In earthworm-invaded plots isotope enrichment was highest in macroaggregates and microaggregates whereas in NW plots silt plus clay fractions were most enriched. The net effect of litter mixing and priming of recalcitrant soil organic matter (SOM), stabilization of SOM in soil aggregates, and alteration of the soil microbial community by earthworm activity results in loss of SOM and lowering of the C:N ratio. We suggest that earthworm stoichiometry plays a fundamental role in regulating C and N dynamics of forest SOM.

Fertilizer 15N Accumulation, Recovery and Distribution in Cotton Plant as Affected by N Rate and Split

N fertilization of 300 kg N ha−1 is normally applied to cotton crops in three splits: pre-plant application (PPA, 30%), first bloom application (FBA, 40%) and peak bloom application (PBA, 30%) in the Yangtze River Valley China. However, low fertilizer N plant recovery (NPR) (30-35%) causes problems such as cotton yield stagnation even in higher N rate, low profit margin of cotton production and fertilizer release to the environment. Therefore, it is questioned: Are these three splits the same significance to cotton N uptake and distribution? An outdoor pot trial was conducted with five N rates and 15N labeled urea to determine the recovery and distribution of 15N from different splits in cotton (Gossypium hirsutum L. cv. Huazamian H318) plant. The results showed that, cotton plant absorbed fertilizer 15N during the whole growing period, the majority during flowering for 18-20 d regardless of N rates (150-600 kg ha−1). Fertilizer 15N proportion to the total N accumulated in cotton plant increased with N rates, and it was the highest in reproductive organs (88% averaged across N rates) among all the plant parts. FBA had the highest NPR (70%), the lowest fertilizer N lose (FNL, 19%), and the highest contribution to the fertilizer 15N proportion to the total N (46%) in cotton plant, whereas PPA had the reverse effect. It suggests that FBA should be the most important split for N absorption and yield formation comparatively and allocating more fertilizer N for late application from PPA should improve the benefit from fertilizer.

The role of green manure nitrogen use by corn and sugarcane crops in Brazil

Three assays were developed from April 3, 1995 to October 10, 2005. The work with corn was conducted in a greenhouse, using velvet bean (Mucuna aterrima) and sunn hemp (Crotalaria juncea) as green manure with 15N labeling of either shoots or roots, in two soils with contrasting textural classes. The mineralization of N from legume plants incorporated into the two soils was investigated too. This work included two green manures: velvet bean and sunn hemp, and the common bean (Phaseolus vulgaris) residues. Nitrogen from the velvet bean accounted for a greater proportion of the soil inorganic N; shoots were responsible for most of N accumulated. Common bean residues caused immobilization of inorganic N. The leguminous species added were intensively and promptly mineralized, preserving the soil native nitrogen. One hundred days after emergence of the corn, velvet bean provided higher accumulation of nitrogen in the soil, higher absorption by corn plants and accumulation in the aerial part. The green manure decomposition was more intense in the medium textured soil. In this soil, highest nitrogen losses were also observed. The sugarcane (Saccharum spp.) was cultivated for five years in the field and was harvested three times; 15N recovery was evaluated in the first two harvests. The combination of inorganic fertilizer and green manure resulted in higher sugarcane yields than either N source applied separately; however, in the second cutting the yields were higher where sunn hemp was used than in plots with ammonium sulfate. The recovery of N by the first two consecutive harvests accounted for 19% to 21% of the N applied as sunn hemp and 46% to 49% of the N applied as ammonium sulfate. Very little inorganic N was present in the 0-40 cm soil layer with both N sources.

Preferential use of root litter compared to leaf litter by beech seedlings and soil microorganisms

Litter decomposition is regulated by e.g. substrate quality and environmental factors, particularly water availability. The partitioning of nutrients released from litter between vegetation and soil microorganisms may, therefore, be affected by changing climate. This study aimed to elucidate the impact of litter type and drought on the fate of litter-derived N in beech seedlings and soil microbes. We quantified 15N recovery rates in plant and soil N pools by adding 15N-labelled leaf and/or root litter under controlled conditions. Root litter was favoured over leaf litter for N acquisition by beech seedlings and soil microorganisms. Drought reduced 15N recovery from litter in seedlings thereby affecting root N nutrition. 15N accumulated in seedlings in different sinks depending on litter type. Root turnover appears to influence (a) N availability in the soil for plants and soil microbes and (b) N acquisition and retention despite a presumably extremely dynamic turnover of microbial biomass. Compared to soil microorganisms, beech seedlings represent a very minor short-term N sink, despite a potentially high N residence time. Furthermore, soil microbes constitute a significant N pool that can be released in the long term and, thus, may become available for N nutrition of plants.

Nitrogen Use by Yam as Affected by Mineral Fertilizer Application

Soil fertility decline and high N demand during plant growth are cited as main causes of low yield in yam (Dioscorea spp.). As information regarding fertilizer N use in yam is lacking, a field experiment was undertaken in 2006 and repeated in 2007 in central Côte d'Ivoire using 15N (15NH415NO3) labeled fertilizers to monitor N uptake and fertilizer use efficiency in D. alata. The 15N‐labeled fertilizer was added in two splits (at 90 and 130 d after planting) for a target tuber yield of 40 Mg fresh tuber ha−1. The application of mineral fertilizers increased total biomass production and tuber yield as well as N uptake from native soil organic matter. The recovery of 15N‐labeled fertilizer applied at 90 and 130 d after planting was not significantly different but the year affected 15N recovery by the plant. The maximum 15N recoveries calculated from the sum of the 15N recovery measured at both application dates were 46 and 23% in 2006 and 2007, respectively. Leaf dry matter and leaf N uptake were higher in 2006 than in 2007, but tuber dry matter was not different between years at the final harvest. Up to 170 kg N ha−1 was returned to the soil through the fallen shoots, indicating an important recycling of N for soil fertility replenishment. Thus, crop succession or intercropping are encouraged in a yam cropping system. Our results also show that the application of mineral fertilizers had a strong “priming effect” on the native soil N in both years.