Recombinant hypoallergenic proteins have been explored for allergy immunotherapy and prophylaxis, while probiotics have demonstrated protective effects against allergic diseases.This study describes the expression of a hypoallergenic Sui p 2 allergen from the house dust mite (HDM) Suidasia pontifica, in the probiotic yeast Saccharomyces boulardii, in order to generate a potential prophylactic agent for HDM allergy.Modified sequence of the Sui p 2 gene was cloned and expressed in S. boulardii (rSb) as a recombinant hypoallergen.The 378 bp hSui p 2 sequence was inserted into the yeast plasmid pYIP-hSui p 2, which underwent digestion with StuI before transformation via high-efficiency LiAc/SS.Gel electrophoresis after StuI restriction enzyme digestion confirmed successful transformation, revealing a 4571 bp plasmid while Polymerase Chain Reaction confirmed the presence of hSui p 2 insert integrated into the genome of Sb.Growth curve analysis indicated that both rSb exhibited a slower growth rate, a delayed peak, and a slightly lower biomass yield compared to the untransformed clone of S. boulardii (Sb).Furthermore, dot blot immunoassay using anti-His antibodies verified the successful expression of rSb.The expression of a recombinant hypoallergen Sui p 2 as an initial step in the development of a potential prophylactic agent for HDM allergy.