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In Vitro sterilization protocol for callus induction of Baccaurea courtallensis (Wight) Müll.Arg. - An endemic tree of Southern Western Ghats, India

Experiments were carried out to establish in vitro culture using terminal and lateral buds of the endemic tree Baccaurea courtallensis of Phyllanthaceae which presented formidable difficulties. Besides the profuse phenol exudation, cultures were infected with intense fungal contamination. Efforts directed to control the schedule by adjusting the concentration of disinfectants NaOCl and HgCl2 with additional rinsing of explants with 70% ethanol and pre-soaking of shoots in various concentrations of the fungicide Bavistin never fetched any success. The intricately connected fungus seems to have found a niche amidst the leaf primordia in shoot apices and the axillary crevices of the nodal regions completely establishing itself in cultures. Perplexed with no remedy in sight, an interesting attempt of quick surface washing with 70% acetone was introduced in the surface sterilization schedule. Fresh explants were quickly rinsed with 70% acetone, followed by 0.1% mercuric chloride, 1% bavistin along with 4% PVP in succession and later wash with sterile distilled water enabling effective establishment of culture. Callus formation was observed on MS and WPM supplemented with auxin and cytokinin. Callus induction from the nodal region was observed at all concentrations and combinations of PGRs supplemented. Maximum induction of callus was obtained from MS medium augmented with BAP 4μM alone and in combination with IBA 1 μM +TDZ 4 μM.

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Plant community patterns and edge effect study in the ecotone region of River Pabber and Tons in Western Himalaya

The present study was carried out in riverine ecotone of Tons and Pabber rivers which are tributary of river Yamuna in Garhwal Himalaya to understand the edge effect on distribution and structure of plant community patterns. The study was done at an altitude of 900 m to 1300 m to identify the plant diversity and community structure of riverine ecotone areas within a stretch of 64 km. In total Seventeen (N=17) tree species twenty one (N=21) shrub species, thirty-eight (N=38) species of herbs and three (N=3) species grasses were identified. Mallotus philippensis, Alnus nepelensis, and Pinus roxburghii were found to be dominant threes having 35.7%, 14.9% and 13.6% representation in total tree population. Out of nine (N=9), sample sites Mallotus philippensis and Pinus roxburghii were having the highest densities and relative densities in five (N=5) sites. Rubus elipticus was dominating shrub species with 16% representation followed by Zanthoxylum armatum 12.2%, Adhatoda vasica 10.3% and Agave cantula 9.3%. Rubus elipticus and Zanthoxylum armatum.were having the highest numbers in sites where the slope was gentle and soil conditions were not sandy. Whereas Adhatoda vasica was more uniformly distributed and was found growing uniformly in riverine sandy soil areas Agave cantula preferred the southern and western aspects with rocky slopes and poor soil conditions. In grasses, Carex infuscata was having highest dominance (43.64), in herbs Trifolium repens was having the highest dominance (21) followed by Cannabis sativa (20.92), Cynodon dactylon (17.8) and Verbascum thapsus (10.71). Species diversity indices used in the study was the Shannon Index, the value of diversity indicated by H' was 2.44 which indicates that the herb diversity in the ecosystem is uniformly distributed, no single herb species is dominant, whereas the dominance value which is 0.11 corroborate the diversity of the ecosystem. The value of Shannon wiener (H') index for shrub species was 1.58 which indicates that the majority of shrub (66.6%) species are distributed uniformly except Berberis lycium, Clerodendrum phlomidis, Euphorbia royleana, Indigofera heterantha, Lantana camara, Woodfordia fruticosa and Viburnum cotinifolium.

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Ethnomycological study of macromycetes used in the Funa district, Kinshasa, Democratic Republic of the Congo

The present study is a contribution to the knowledge of fungal species of food interest exploited by the population of Funa district, in Kinshasa city in the Democratic Republic of the Congo. The aim was to assess the knowledge of Funa district community in mushroom species diversity sold in the market. The mycological surveys were conducted between December 2019 and January 2020. In the study, we used survey-participation technique. A survey-participation was used as a technique, which is a non-probabilistic method where the interviewees were randomly selected. The sample size was of 81 participants. Qualitative and quantitative methods supported by ethnomycological surveys were used to collect data. Out of 81 people interviewed, 97.5% were women; food use was predominant with 93.8%; 80.2% of participants reported that mushrooms were difficult to preserve due to their perishable nature; and 96.2% of participants felt that mushrooms were only available during the dry season. The sale of sporophores in FUNA District remains the only method of supplying households and local markets. Nevertheless, mushrooms are a valued commodity in women's trading activities in Funa District. More studies are needed to have a clear view on different species consumed by the Kinshasa people and create a database of edible mushrooms consumed in DRC. Furthermore, it is required to assess the knowledge of different Congolese tribes to increase the knowledge of ethnomycology and assess their in vitro medicinal activities against different pathologies.

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