Abstract Purpose of Review Human pathogenic fungi are increasingly recognized as major threats to human health, yet their complex biology and interactions with the host remain poorly understood. This review highlights the role of image-based systems biology in fungal infection research, emphasizing how computational models bridge experimental observations and mechanistic understanding. Recent Findings Recent advances in imaging, quantitative image analysis, and mechanistic modeling have enabled dynamic and precise characterization of fungal infections. Integrative studies in Candida albicans and Aspergillus fumigatus have linked measurable cellular behaviors to infection outcomes, revealing how fungal growth, immune evasion, and host responses jointly determine disease progression. Computational models now reproduce infection dynamics, identify key parameters shaping immune control, and guide therapeutic strategies. Summary Coupling quantitative imaging with computational modeling transforms fungal systems biology from descriptive observation to predictive and mechanistic insights, enabling the rational design of diagnostics and therapeutic strategies.

Abstract Purpose of Review Bacteria are highly adaptive organisms that have evolved various survival strategies to persist and thrive in hostile environments. They especially form polymicrobial biofilms with an intricate microbial community network in a highly regulated fashion. This review covers recent findings on bacterial biofilm pathogenesis and innovative multidisciplinary therapeutic interventions for clinical infections. Recent Findings Bacterial cyclic dimeric guanosine monophosphate (cyclic di-GMP) is a central regulator for biofilm pathogenesis orchestrated by quorum sensing (QS) molecules, allowing prolonged survival, host immune evasion, antibiotic resistance, and augmented virulence with severe health consequences. Biofilms associated with medical devices or wounds pose significant risks due to sessile bacteria, which are notably more resistant to eradication than their planktonic counterparts due to diminished metabolic activities. This phenomenon complicates the treatment of biofilm-associated infections and exacerbates the persistence of chronic illnesses. Novel intervention strategies include CRISPR/Cas9, QS inhibitors, natural antimicrobial peptides, probiotics, antimicrobial nanomaterials, and advanced biophysical remedies. Summary A deeper understanding of the regulation of biofilm pathogenesis will facilitate the development of novel interventions suitable for clinical biofilms in various chronic infections.

Abstract Purpose of Review Entamoeba histolytica is the primary causative agent of amebiasis, with transmission occurring mainly through contaminated food and water. A possible, though secondary, zoonotic component has been identified, involving animal reservoirs, primarily non-human primates and dogs. Recent Findings Recent studies indicate that various amoebae species can colonize the human gut, and specific strains may have animal reservoirs capable of maintaining their life cycle and facilitating zoonotic transmission. Summary This review highlights the importance of animal-to-human transmission of pathogenic, potentially pathogenic, and non-pathogenic amoebae species, and discusses their implications from a public health perspective.