Naphelium lappaceum L or Rambutan in Indonesian, fruit peel has been identified as a potential source of polyphenolic compounds, including ellagic acid, geraniin, and corilagin. Ellagic acid has been reported to have strong antioxidant activity, inhibit adipogenesis, increase lipid oxidation, and reduce plasma lipid concentrations in obese animal models. This study aimed to analyze the response of ellagic acid contained in N. lappaceum peel extract on lipid profiles and liver histology in obese rat models. Twenty-five male Sprague-Dawley rats were divided into five groups: normal (P1), obese (P2), ellagic acid (P3), O-RPE (Obese rats treated with RPE) 15 mg/kgBW (P4), and O-RPE 30 mg/kgBW (P5). Treatment was administered for 30 days. The results showed that administration of ellagic acid and RPS (O-RPE) significantly improved metabolic parameters in obese rats. ANOVA analysis revealed lower LDL (p < 0.05), reduced triglycerides, and improved liver histology scores in treatment groups compared to the obese control, while other parameters showed favorable but non‑significant trends. In conclusion, ellagic acid in RPE demonstrates potential as a herbal anti-obesity agent by improving lipid profiles and liver tissue structure. This research highlights the innovative use of N. lappaceum peel as a source of bioactive compounds for obesity management, an area that remains underexplored. This research supports SDGs 3 (Good Health and Well‑being) by offering natural interventions for obesity, and SDGs 12 (Responsible Consumption and Production) by promoting sustainable use of agricultural waste.

The cultivation of Pleurotus ostreatus mushrooms has gained popularity in the Philippines because of its ability to grow across a wide range of temperatures and to utilize diverse lignocellulosic substrates. However, optimizing culture conditions is essential for improving the growth efficiency of sporeless strains, which are preferred due to reduced health risks and better quality of mushroom products. This study aimed to determine the optimum cultural conditions for the mycelial growth of P. ostreatus (sporeless strain) by evaluating the effects of media type, pH level, aeration (sealed vs. unsealed plates), illumination (dark, light, or alternating dark/light), and incubation temperature. Mycelial growth was assessed across five different culture media and varying environmental conditions. Results revealed that rice bran decoction gulaman supported the most favorable growth, with near-neutral to slightly basic pH, under sealed or non-aerated conditions. Growth was consistent across dark, light, or alternating illumination, and was optimal at both room temperature and air-conditioned settings. These findings demonstrate that the sporeless strain exhibits strong adaptability to diverse conditions, reducing cultivation constraints compared with spore-forming counterparts. This research establishes the specific cultural requirements for the sporeless strain, which has been less studied despite its advantages. Optimizing its growth conditions can enhance mushroom production efficiency, minimize spore-related health risks for growers, and promote sustainable utilization of local agro-industrial byproducts, contributing to food security and rural livelihood development. This research supports SDGs by contributing to SDGs 2 (Zero Hunger) through improved mushroom production efficiency and food security; SDGs 3 (Good Health and Well-being) by reducing spore-related occupational health risks; SDGs 8 (Decent Work and Economic Growth) by strengthening livelihood opportunities in small-scale enterprises; SDGs 12 (Responsible Consumption and Production) through the sustainable use of agro-industrial byproducts; and SDGs 13 (Climate Action) by promoting resource-efficient cultivation practices aligned with the sustainability framework of the United Nations Sustainable Development Goals.

Jember Regency encompasses several plantation areas characterized by diverse ecological conditions, notably the Gumitir, Garahan, and Tancak plantations. Among the flora in these regions, the genus Hoya is notable for its medicinal potential. This study aimed to characterize the diversity of Hoya species, provide detailed morphological documentation, and evaluate the phytochemical and antioxidant properties of a medicinally used species. Field exploration employed a systematic plot-based design consisting of 150 plots (5 × 5 m²) with 3 m spacing. The most vegetatively dominant species, Hoya lacunosa, was subjected to GC–MS phytochemical analysis and DPPH-based antioxidant evaluation. A total of eleven Hoya species were recorded across the three plantation sites. The Gumitir plantation exhibited the highest diversity, containing ten species., i.e. Hoya burtoniae, H. camphorifolia, H. carnosa, H. cinnamomifolia, H. dennisii, H. fuscomarginata, H. lacunosa, H. micrantha, H. purpureofusca, and two unidentified Hoya spp. In contrast, the Garahan plantation supported only H. dennisii and H. fuscomarginata, while H. lacunosa was exclusively found in Tancak indicating a highly localized distribution. GC-MS profiling on H. lacunosa leaves revealed 26 compounds, comprising 16 secondary metabolites (12 phenolics, 2 alkaloids, and 2 terpenes) and 5 primary metabolites. The methanol extract exhibited strong antioxidant activity (IC₅₀ = 50.89 ppm), reflecting its potential as a natural source of bioactive compounds. These findings demonstrate substantial Hoya diversity in Jember Regency and underscore the potential of H. lacunosa as a valuable source of bioactive metabolites for pharmaceutical development.

Salinity stress on productive rice fields in coastal areas will have a negative impact on productivity through osmotic stress and nutrient deficiencies. Salinity stress causes high levels of Na and Cl in the soil, thus inhibiting phosphate absorption. Utilization of indigenous P-solubilizing bacteria from saline land is an alternative, environmentally friendly technology. The purpose of this study was to isolate and characterize P-solubilizing bacteria from saline rice fields. Soil samples were taken from the rice rhizosphere in Nyamplungsari Village, Peraturkan District, Pemalang Regency. Isolation and characterization were carried out at the Agronomy & Horticulture Laboratory, Faculty of Agriculture, UNSOED. The variables observed included P solubility index, P solubilizing ability, IAA production, and bacterial identification using the 16S RNA method. The results of the study obtained 7 isolates of P-solubilizing bacteria that had the ability to solubilize P and produce IAA. Isolate KF is a P-solubilizing bacterium that has the highest P-solubilizing ability and is identified as Priestia megaterium strain NRRL B-350. Priestia megaterium strain NRRL B-350 is a species of P-solubilizing bacteria that has the potential to be developed as a specific biological fertilizer for saline soil to increase the growth and yield of rice plants under saline stress.

Catastrophic mortality occurred in the fish community at Serayu Movable Dam in 2022. The phenomenon was caused by severe water quality alteration due to sediment flushing from Panglima Besar Soedirman Dam. After the incident, the fish community in the reservoir has never been evaluated. This study aimed to assess the fish community status in the Serayu Movable Dam post-catastrophic mortality in 2022. The study was conducted using a survey method, and fish samples were collected three times at six sampling sites at an interval of two weeks. Fish identification was done using the identification key in the references. Various community and water quality parameters were measured. The data obtained were analyzed descriptively by comparing them with standards in the literature. A total of 27 species were identified with a richness index of 4.564, relative abundance of 0.037, diversity index of 2.111, evenness index of 0.693, and dominance index of 0.259. The data proved that the fish community in Serayu Movable Dam has recovered after two years of catastrophic mortality in 2022. It could be due to the characteristic of the riverine ecosystem that can revert due to the continuous replacement of water from various tributaries that flow into the Serayu River in the upstream part of the Serayu movable dam, as shown by good water quality. It can be concluded that high ichthyofauna diversity was observed in the Serayu Moveable Dam post-catastrophic mortality. The data are essential scientific bases for sediment flushing management at P.B. Soedirman Dam and management of freshwater fish community in Serayu Movable Dam.

Coastal ecosystems are synonymous with mangrove trees. Threatened mangrove existence is tantamount to reduced biological existence, weakened coastal protection, and reduced carbon storage. Moreover, mangrove growth naturally depends on the quality of seedlings supported by proper nursery management. This study aims to evaluate the impact of four environmental factors (light intensity, salinity, pH, and tidal frequency) on the growth and survival of Rhizophora: Rhizophora mucronata, Rhizophora stylosa, and Rhizophora apiculata cultivated in nurseries with a water system integrated with the Internet of Things (IoT). A single-factor design was applied, with each treatment bed containing 600 seedlings (200 per species) and an uncontrolled bed as a control. Weekly random sampling of 20% of seedlings from each treatment for eight weeks. Data were analyzed using a two-way ANOVA with treatment and species as fixed factors, followed by the Tukey HSD test at a significance level of 5%. The results showed that R. mucronata in the light treatment (without shade net) achieved the highest survival rate (98.8%) and the largest stem diameter, which was 0.698 cm. In addition, the highest seedling height was R. mucronata in the high-yield salinity treatment, reaching 56.78 cm, and the highest number of leaves was recorded in R. apiculata in the salinity treatment, with an average of 6.92 leaves per seedling. IoT-integrated nurseries stabilize water supply and tidal cycles, reduce mortality to below 10%, and enhance seedling quality. These findings indicate that nursery environmental management can improve the consistency of mangrove seedling growth and support large-scale restoration.

Identifying pathogenic fungi at the species level based on morphology, especially for leaf rust, is quite difficult. Further investigation, such as molecular techniques, is required to identify leaf rust fungi precisely. One way that can be employed is DNA barcoding. This study seeks to detect leaf rust fungi (Puccinia spp.) on sugarcane. Accurate species identification allows early detection of Puccinia kuehnii, helping farmers and extension agencies to apply targeted control strategies. A fungal sample was collected from sugarcane fields in Bondowoso, East Java, Indonesia. Morphological identification was done with an SEM, whereas molecular identification was done through DNA extraction, amplification, visualization, sequencing, and phylogenetic tree creation. The primers employed are obtained from the ITS region of ribosomal DNA, which contains enough diversity to differentiate fungi at the species level. The amplification findings revealed a DNA band of 500 bp at both loci. Sequencing results indicate that samples The sequencing results indicatethat the samples from Bondowoso are closely related to the Puccinia kuehnii sequence from NCBI. The findings of the phylogenetic tree construction revealed that samples from both locations are still associated with P. kuehnii sequences from other nations. This is the first study documenting P. kuehnii in Indonesia, especially East Java, that integrates morphological and molecular characterization.

Bisphenol-A (BPA) is classified as an endocrine-disrupting chemical that is widely found in everyday food. However, various studies have reported inconsistent results regarding the effects of this exposure on behavior. This study aimed to evaluate how dose, genetic background, and duration of exposure interact to shape behavioral responses in Drosophila melanogaster exposed to BPA. Three strains (Wild-type, white-eye, and black-body) were cultured on a medium containing 0, 0.25, and 0.5 mg/mL BPA for two generations. Before exposure (G0), after the first generation of exposure (G1), and after the second generation of exposure (G2), three behaviors were measured: larval crawling speed, number of larval contractions, and larval exploration distance. Repeated-measures ANOVA revealed a non-monotonic pattern in behavioral responses. Each behavior exhibited distinct sensitivities to treatment factors, and complex interactions were observed between genetic factors, dose, and time (generation). Furthermore, the effects of BPA are not general but rather specific to the type of behavior observed. Generational effects are evident in some behavioral data. In addition, fly strain plays a role in determining the effect of exposure on behavioral responses.

The conservation of Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth. The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf. Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.

Lopang (Gymnopetalum cochinchinense) is a herbaceous plant traditionally consumed as a vegetable by communities in Riau Province, Indonesia. However, DNA barcode sequence data for this species remain limited. These findings underscore the importance of analyzing DNA barcode sequences of matK and trnL-trnF intergenic spacer (IGS) lopang from Riau. This study highlights the analysis of DNA barcode sequences matK and trnl-trnf intergenic spacer (IGS) in lopang from Riau province. This study applies sampling, DNA extraction, Polymerase Chain Reaction (PCR), electrophoresis, sequencing, and bioinformatics data analysis using BioEdit 7, BLASTn (Basic Local Alignment Search Tool) to find sequence similarities with the GenBank database, Management and analyzed DNA sequences with MESQUITE, Multiple alignments using ClustalW and MEGA11 to create dendrograms. Fresh leaves were collected from Rokan Hulu Regency in Riau Province. The results showed that matK and trnL-trnF IGS DNA sequences of lopang measured 752 bp and 410 bp, respectively. BLASTn analysis revealed that lopang has 99.73% similarity with G. chinense based on the matK sequence and 99.76% similarity based on the trnL-trnF IGS sequence. The analysis revealed a variation of 13 nucleotides, 1 critical nucleotide and no indels in the matK sequence, while in the trnL-trnF IGS sequence there were 19 nucleotide variations, 1 critical nucleotides and 5 indels. As a final point, lopang from Riau is closely related to G. chinense. The findings of this research contribute to the molecular identification of this species and benefit science, such as providing an understanding of plant evolution, species identification, genetic analysis, and the development of molecular markers in the Cucurbitaceae family.