BackgroundSphingomyelin synthase (SMS) is the only enzyme that synthesizes sphingomyelin from ceramide. The role of sphingomyelin synthase in epidermis is being understood, but there is no report on its role in the dermis. Quantitative and qualitative evaluation of collagen in SMS2-deficient mice reveals the role of SMS2 in collagen production.MethodsSMS2-deficient mice were used for in this study. The dermis thickness was measured by Elastica van Gieson staining, the collagen fiber was observed by Scanning Electron Microscopy, the collagen content by ELISA, the ceramide and sphingomyelin content by Thin Layer Chromatography, the collagen-generating and metabolizing gene expression level by RT-PCR, and MMP13 protein level was measured by western blotting.ResultsThinner dermis in these mice compared to wild-type mice. A reduced number of collagen fibers were observed, and decreased levels of type I collagen and sphingolipids. Gene expression levels of collagen production-related genes in the dermis were found to be unaltered. The expression of several genes related to collagen degradation was found to be affected. The expression level of TNFα and MMP13 and MMP13 protein levels were increased relative to those of wild-type mice, while the expression level of TIMP1 was decreased.ConclusionsThese results indicate that SMS is involved not only in maintaining the sphingolipid content of the epidermal barrier but also in maintaining collagen homeostasis. Further elucidation of the role of SMS2 in the skin may lead to SMS2 comprising a new target for the treatment of skin diseases and the development of functional cosmetics.

Hypertrophic scars (HTS) are raised, red, rigid, inflexible cell-like, and cosmetic problems precipitated due to multiple underlying dermal injuries such as burn, surgery, and trauma during which aberrant wound healing with more pathological deposition of the extracellular matrix than degradation leads to their spawning. Till date, well established and specific treatments for HTS have not been reported; hence, the need of recent developments is thrusted with novel drug delivery vision. This review will try to encompass all the agogs to HTS, definition, pathophysiology, mechanism of hypertrophic scar formation, the role of growth factors in hypertrophic scarring, and their difference with keloids. Further, it will illuminate the available medicaments and recent advances in novel topical drug delivery systems such as ethosomes, transethosomes, liposomes, solid lipid nanoparticles, and microsponges for treatment of HTS.

The fatty acids containing plant oils have been used as components of cosmetic formulations, and the best cosmetic function has been established for unsaturated fatty acids present in triglycerides. Many oils, fats, waxes, and phospholipids are employed as excipients in cosmetics and personal care products. Antioxidants such as carotenoids, retinoids, and tocopherols are used for their antioxidant properties because of their importance in therapeutic care. For skin care, the most important are oils with high contents of linoleic and α-linolenic acid which reduce the formation of eczemas or atopic dermatitis. These essential fatty acids incorporate into the cell membranes and regenerate the damaged lipid barrier of epidermis and restrict water loss. The unsaturated fatty acids show prominent healing effects on skin inflammation and are used in various cosmetic products. Mineral oils and waxes are also used in several products due to their property of skin tolerance, protection, cleansing, and viscosity. As cosmeceutical formulation, the nanoformulations are effective against irritated and inflamed skin and effective for therapy of atopic dermatitis and psoriasis. The lipid nanoparticles offer advantages of chemical stability and good skin compatibility that can be used for protection against radiation and aging.

BackgroundMelanin is a pigment, which is widely distributed in organisms. In humans, melanin pigments determine eye, hair, and skin color and protect the skin from damage by ultraviolet radiation; however, immoderate distribution of melanin in the skin causes discoloration. In the present study, we screened methanolic extracts from leaves of 47 plant species, most of which are native to East Asia, for the inhibitory activity against melanogenesis.MethodsB16 melanoma 4A5 cells were used in all assays conducted in this study. Melanin content assay was performed using methanolic extracts from leaves of 47 plant species. Cytotoxicity of the extract from leaves of Sorbaria sorbifolia var. stellipila Max. (SME) was evaluated by WST-8 assay. Tyrosinase activity was determined using the lysate of α-MSH-stimulated B16 melanoma 4A5 cells and L-dopa as a colorimetric substrate. Melanogenic gene expression was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).ResultsSME showed the highest inhibitory activity among tested samples without cytotoxicity. SME exhibited the inhibition potency for the enzymatic activity of tyrosinase. In addition, qRT-PCR result displayed that SME downregulates the expression of melanogenic genes including tyrosinase, TRP-1, TRP-2, and the transcription factor MITF.ConclusionOverall results revealed that the extract from leaves of S. sorbifolia var. stellipila Max. has a potential to be used as a skin whitening agent.

BackgroundHIV infection and meningitis are diseases which are known to precipitate malnutrition. Skin manifestations of malnutrition are easy to identify. We tried to describe the skin manifestations of malnutrition in HIV-positive patients with meningitis.SettingPatients included in the study were from a tertiary referral hospital in South India.MethodsIn a cross-sectional observational design, we studied 56 adult [> 14 years] HIV-seropositive patients with meningitis by clinical assessment of skin manifestations of malnutrition.ResultsSkin wasting (31.5%), hyperpigmentation of skin (22.2%) and skin ulceration (16.4%) were the chief skin manifestations of malnutrition in HIV-positive patients with meningitis.ConclusionsOur study shows that certain cutaneous features of malnutrition like skin ulcers, skin wasting and hypo pigmentation are common in HIV patients with meningitis.

Transdermal delivery systems have been intensively studied over the past 2 decades, with the focus on overcoming the skin barrier for more effective application of pharmaceutical and cosmetic products. Although the cosmeceutical industry has made a substantial progress in the development and incorporation of new and effective actives in their products, the barrier function of the skin remains a limiting factor in the penetration and absorption of these actives. Enhancement via modification of the stratum corneum by hydration, acting of chemical enhancers on the structure of stratum corneum lipids, and partitioning and solubility effects are described. This review summarizes the advances in the development and mechanisms of action of chemical components that act as permeation enhancers, as well as the advances in appropriate vehicles, such as gels, emulsions, and vesicular delivery systems, that can be used for effective transdermal delivery.

BackgroundBurns are injuries that lie on the skin or other organic tissues caused by exposures to the heat, electricity, chemicals or ionizing radiation. The present study was carried out to record temporal changes in the cell population and wound healing-related gene expression in rats with deep partial-thickness burn.MethodsBurn wound was induced on the dorsal part of Sprague-Dawley rats using temperature-regulated 20-mm wide aluminum head heating device. Animals were then sacrificed on days three, seven, 11, 14 and 21 post-burn, respectively. Half of the wounded skin tissues were dissected and fixed in buffered neutral formalin for Hematoxylin & Eosin (H&E) staining, and the other half were cut off and stored in − 20 °C for real-time PCR analyses.ResultsThe number of adipose cells was found to be maximal on the 3rd day post-burn, and it gradually decreased over time and completely disappeared on day 11 post-burn. The maximum number of neutrophils were found to be on the 3rd and 14th day post-burn, while the maximum number of myofibroblasts were found on the 11th day post-burn. The number of lymphocytes did not change too much during the whole healing process. At the gene expression level, the expression pattern of inflammation-related genes including IL-6, TNF-α and iNOS were similar, which was found to be increased from day 3 to day 11 and decreased thereafter. Angiogenesis related genes including both VEGF-A and TGF-β1 showed a same expression pattern, both of which were slightly increased from day 3 to day 14 and smoothly decreased on day 21 post-burn. Matrix re-modeling related genes including MMP-2, TIMP-2 and Collagen-1 changed over time synchronously, where they all persistently increased from day 3 till day 14, then slightly declined on day 21 post-burn.ConclusionThe present study revealed the changes in the cell population and expression profile of wound healing-related genes in deep partial-thickness burn, which could provide a cellular and genomic basis for the future research of burn injuries.

BackgroundWith the steadily increasing trend of functional cosmetics consumption, the public is increasingly expecting the efficacy of cosmetics, and in order to meet these consumer needs, the development of various effective raw materials, as well as products through technical convergence research, has been activating focusing on dermatology. In the Republic of Korea, much attention was paid to cosmetic raw materials using human stem cells as a new growth engine at the end of the 2000s. Accordingly, the Ministry of Food and Drug Safety implemented the Regulations on Cosmetic Safety Standards, etc., which contains the safety standards of human-derived cell and tissue culture as raw materials for cosmetics.MethodsTwice a day, morning and evening, for 28 days, 3 mL of the sample provided for each subject was applied onto the entire face using an airbrush. The subjects were divided into a control group (C), an experimental group 1 (ADSC-CM 5% group, E1) and an experimental group 2 (polymersome-containing of ADSC-CM 5% group, E2), and there were 20 subjects in each group.ResultsMoisture content on the U-zone, melanin and erythema of lid-cheek, overall size and depth of eye wrinkles, and skin roughness of the items showed significant differences among all three groups. More specifically, E1 had a higher rate of skin improvement than C, and E2 had the highest skin improvement rate in all items: skin moisture content, transepidermal water loss (TEWL), melanin and erythema of lid-cheek, eye wrinkles, and skin roughness of lid-cheek.ConclusionsThe effectiveness of ADSC-CM was verified in all items: increases in moisture content, decrease in TEWL, and improvements of whitening and wrinkles. Moreover, the effectiveness of ADSC-CM was observed as higher in samples of polymersome-containing ADSC-CM than those of raw material ADSC-CM. Furthermore, percutaneous absorption was improved compared with previous studies on formulations in relation to polymersome at the same concentration of ADSC-CM, thereby resulting in higher effectiveness.Trial registrationKorea National Institute for Bioethics Policy, P01–201809–13-001, Registered 06 September 2018, http://public.irb.or.kr/.

BackgroundUsing human keratinocyte HaCaT cell line model, we screened for proteins that changed their content due to SDS exposure in non-toxic dose (25 μg/ml, as determined by the MTT assay and microscopic examination) during 48 h.MethodsThe altered level of proteins from HaCaT keratinocytes exposed to SDS was analyzed by LC-MS/MS approach and quantified using Progenesis LC software.ResultsThe Pathview map of 131 upregulated proteins was built, and enhancement of glycolysis/gluconeogenesis was found.ConclusionsThe results of our study admit the possibility of promotion of the cutaneous neoplasia and/or the peculiarity of the response of immortalized keratinocytes to the SDS treatment and provide new insights into possible role of SDS as integrator of diverse signaling that influence cell fate decisions.

BackgroundTrichofolliculoma of the eyelid and periorbital region remains is rare, and there is limited evidence regarding surgical management, clinical and histological margins, and recurrence rate.ObjectivesWe performed a systematic review of cases to date on diagnosis and management outcomes of eyelid trichofolliculoma.MethodsElectronic databases were searched for cases reported on trichofolliculoma of the eyelid. The authors extracted and collated data from these studies, and outcomes were summarized descriptively.ResultsOne hundred twenty citations were identified from database searching. The authors also report a case of eyelid trichofolliculoma. Fifteen studies reporting 19 cases of eyelid trichofolliculoma were finally included. Eighteen out of 19 (94.7%) cases were treated with surgical excision. Of 5 cases which were followed up over an average of 2 years, there was only 1 case of recurrence requiring wedge resection.ConclusionsRecurrence rate in patients with eyelid trichofolliculoma excised with satisfactory margins appears to be low, and no cases of malignant transformation have been reported.