ABSTRACT Transgene silencing is a common phenomenon observed in Caenorhabditis elegans, particularly in the germline, but the precise mechanisms underlying this process remain elusive. Through an analysis of the transcription factors profile of C. elegans, we discovered that the expression of several transgenic reporter lines exhibited tissue-specific silencing, specifically in the intestine of C. elegans. Notably, this silencing could be reversed in mutants defective in endogenous RNA interference (RNAi). Further investigation using knock-in strains revealed that these intestine-silent genes were indeed expressed in vivo, indicating that the organism itself regulates the intestine-specific silencing. This tissue-specific silencing appears to be mediated through the endo-RNAi pathway, with the main factors of this pathway, mut-2 and mut-16, are significantly enriched in the intestine. Additionally, histone modification factors, such as met-2, are involved in this silencing mechanism. Given the crucial role of the intestine in reproduction alongside the germline, the transgene silencing observed in the intestine reflects the self-protective mechanisms employed by the organisms. In summary, our study proposed that compared to other tissues, the transgenic silencing of intestine is specifically regulated by the endo-RNAi pathway.

Mobile edge computing (MEC) provides reliable solutions for networked vehicles, mobile phones, and other mobile devices to complete intensive computing and delay-sensitive tasks. Most of the existing studies design a series of methods to achieve the expected goals based on assuming that users are absolutely rational. However, due to the subjectivity of users, the actual results will deviate from the real situation. Using the prospect theory (PT) framework, this article studies the problem of computing task offloading in real situations. Aiming at the task offloading problem in the small cellular network scenario, the artificial fish swarm algorithm is used to optimize the system energy with limited delay. Finally, the experimental tests verify the impact of user behavior on the system energy optimization and the effectiveness of the task offloading method proposed in this article.

Edge computing technology can be used to the Internet of Vehicles (IOV) to solve the mobile characteristics of vehicles and the limited communication range between roadside units and vehicles. New method of edge computing based data adaptive return in IOV is proposed in this paper. The transmission strategy can be determined by adaptive estimating the vehicle movement, the amount of data returned, the maximum transmission delay and effective life of link. And the factors such as speed, direction and position of the vehicles are comprehensively considered and these factors can be measured by the stability effect value when adaptive designing the auxiliary transmission strategy. At the same time, greedy selection method (GSM) is used when constructing the data return link, and the neighbor node as the relay node with the maximum stability and efficiency value is chosen. Our experimental results show our method in terms of performance on transmission delay & packet delivery rate is better than other ones.

Sarcopenia is an age-related condition characterized by a decrease in muscle mass and a decline in muscle strength.Sarcopenia increases the risk of falls,severely affecting the quality of life of patients,and it may be associated with various age-related chronic diseases.Advanced glycation end products(AGEs)are a class of stable glycation products produced by condensation,rearrangement,cleavage,and oxidative modification between the free amino groups of proteins,lipids or nucleic acids and the free carbonyl groups of reducing sugars.Studies have revealed associations of AGEs with muscle mass,muscle strength,and sarcopenia.AGEs can lead to hardening of the extracellular matrix of skeletal muscle through glycation cross-linking.The binding of AGEs to receptors induces inflammation and oxidative stress,consequently resulting in decreases in muscle mass and muscle strength.Therefore,AGEs may play a role in the occurrence and development of sarcopenia.This review summarizes the role of AGEs in the pathogenesis of sarcopenia,offering theoretical support for probing into the mechanisms underlying sarcopenia.

Abstract The aim of this study was to investigate the effects of PAP induced by different squat amplitudes on squat jump and the interaction between squat amplitude, stimulus load (%1RM) and PAP duration, and to establish an exercise intervention programme. Method: Fifteen track and field athletes aged 20-22 years were selected to perform barbell back squats at knee angles of 60°, 90° and 120° and loads of 75% 1RM, 85% 1RM and 95% 1RM, and their flat squat heights were tested at the 3rd, 6th, 9th and 12th minute after each combination of barbell back squats. Results: (1) The 120° knee angle-95%1RM combination produced the best squat jump performance after stimulation (42.47±5.26cm), combinations close to this result include the 120° knee angle-85%1RM (42.27±4.70cm) and the 90° knee angle-95%1RM (42.40±5. 50cm); (2) when load and interval were the same, the effect of different knee angles on squat performance was 120 knee angle > 90 knee angle > 60 knee angle, and when knee angle and interval were the same, the effect of different loads of 1RM on squat performance was 95%1RM>85%1RM>75%1RM, with a high probability of peak occurring at the 9th minute after stimulation. Conclusion: Athletes participating in lower limb explosive power events such as the high jump and long jump should perform a PAP induction with a back squat at a knee angle of 90°-120° and 85-95% of 1RM weight 6-12 minutes prior to competition.

Particulate matter (PM) has deleterious consequences not only on the respiratory system but also on essential human organs, such as the heart, blood vessels, kidneys, and liver. However, the effects of PM inhalation on skeletal muscles have yet to be sufficiently elucidated. Female C57BL/6 or mt-Keima transgenic mice were randomly assigned to one of the following four groups: control (CON), PM exposure alone (PM), treadmill exercise (EX), or PM exposure and exercise (PME). Mice in the three-treatment group were subjected to treadmill running (20 m/min, 90 min/day for 1 week) and/or exposure to PM (100 μg/m3). The PM was found to exacerbate oxidative stress and inflammation, both at rest and during exercise, as assessed by the levels of proinflammatory cytokines, manganese-superoxide dismutase activity, and the glutathione/oxidized glutathione ratio. Furthermore, we detected significant increases in the levels of in vivo mitophagy, particularly in the PM group. Compared with the EX group, a significant reduction in the level of mitochondrial DNA was recorded in the PME group. Moreover, PM resulted in a reduction in cytochrome c oxidase activity and an increase in hydrogen peroxide generation. However, exposure to PM had no significant effect on mitochondrial respiration. Collectively, our findings in this study indicate that PM has adverse effects concerning both oxidative stress and inflammatory responses in skeletal muscle and mitochondria, both at rest and during exercise.

Athletes in the Winter Olympic Games are subject to high injury rates given the physical demands of sports. Comprehensive data regarding injury characteristics in these athletes are limited. To summarize and analyze data regarding the incidence and characteristics of sports injuries occurring in the Winter Olympic Games. Scoping review; Level of evidence, 4. A systematic review of the PubMed, EMBASE, Web of Science, and China National Knowledge Infrastructure databases was conducted. Included were studies reporting the incidence of sports injuries during the Winter Olympics Games from 1995 through 2021. From 168 studies initially retrieved, 4 studies (8824 athletes, 1057 injured athletes) were included. A single-group meta-analysis of sports injury characteristics was performed, with subgroup analysis performed according to the different sports, injury locations, and injury types. Injury severity (time lost from sport) and mechanism were also assessed. The overall injury incidence rate (IIR) during the Winter Olympic Games was 9.6% (95% CI, 4.1%-19.8%). Snow sports were associated with the highest IIR (11.3%), with the top 3 events being the snowboard cross event in snowboarding (31.4%), the aerials event in freestyle skiing (28.6%), and the slopestyle event in snowboarding (27.7%). The most common injury locations were the knee (IIR = 20.0%; 95% CI 17.9%-22.0%), head (IIR = 10.6%; 95% CI, 9.4%-11.9%), and ankle (IIR = 8.2%; 95% CI 7.8%-8.7%). The most common injury types were contusion/hematoma/bruise (IIR = 29.9%; 95% CI 29.7%-30.0%), sprain (dislocation, subluxation, instability, ligamentous, rupture) (IIR = 21.9%; 95% CI 21.4%-22.3%), and strain (muscle rupture, tear, tendon rupture) (IIR = 11.3%; 95% CI 11.0%-11.6%). Regarding injury severity, most athletes had no time lost from sport (64.5%); 24.0% lost fewer than 7 days, and 11.5% lost more than 7 days. The most common injury mechanism was noncontact-related injury (63.3%). In Winter Olympics sports, snow-sport injuries were more common than those associated with other sports, and the most common injury location was the knee. Most injuries did not require time loss, and the most were noncontact-related injuries.