The synthesis of a new series of long-chain arylpiperazine as serotoninergic ligands (FG 1-18) is described. The combination of structural elements including heterocyclic nucleus, propyl chain, and 4,5-dihydrothiazol-2-ylphenylpiperazines leads to the preparation of different derivatives tested for their affinity toward 5-HT1A, 5-HT2A, and 5-HT2C receptors. The compounds with better affinity and selectivity binding profiles toward 5-HT1A and 5-HT2C (FG-1, FG-4, FG-5, FG-6, FG-7, FG-8, and FG-18) are selected for further in vivo assays to determine their functional activity. Finally, to rationalize the obtained results, molecular docking studies are performed. The results of pharmacological studies show that compounds FG-1, FG-5, FG-8, and FG-6 exert antidepressant-like effects, and FG-1, FG-18, FG-6, and FG-7 reveal also significant anxiolytic properties. Among the developed derivatives, the most promising compounds seem to be FG-1, which exhibit antidepressant, anxiolytic, and anticonvulsant properties, FG-7 and FG-18 that show features as anxiolytic combine to a pro-cognitive property and notable affinity and selectivity for 5-HT2C receptor, respectively.

ABSTRACTCardiovascular diseases are a major global health concern, and avocados, rich in monounsaturated fats and bioactive compounds, may help improve heart health by influencing lipid profiles and other risk factors. However, existing studies on avocado consumption and cardiovascular benefits show inconsistent results, and no comprehensive meta‐analysis has been conducted. This study aims to systematically review and analyze current research to provide a quantitative assessment of avocados' effects on cardiovascular risk factors in adults. From inception until May 2025, a comprehensive search was conducted on PubMed, Web of Science, and Scopus to find randomized controlled studies (RCTs) evaluating the effectiveness of avocado intake on cardiovascular risk factors. Following screening, data were extracted and analyzed by STATA. The pooled analysis of 10 RCTs showed that avocado intake had no significant change on triglycerides (TG) (WMD: 0.02 mg/dL; p = 0.97), total cholesterol (TC) (WMD: 1.28 mg/dL; p = 0.62), high‐density lipoprotein (HDL) (WMD: −0.27 mg/dL; p = 0.64), fasting blood glucose (FBG) (WMD: −0.05 mg/dL; p = 0.78), body mass index (BMI) (WMD: −0.07 kg/m2; p = 0.31) and C‐reactive protein (CRP) (WMD: 0.02 mg/dL; p = 0.06). Conversely, a significant reduction was observed in low‐density lipoprotein (LDL) (WMD: −3.75 mg/dL; p < 0.001; I2 = 0%), systolic blood pressure (BP) (WMD: −1.15 mmHg; p = 0.03; I2 = 56%), and an under border of insignificant change in diastolic BP (SWD: −0.03 mmHg; p = 0.066; I2 = 61.9%). The findings from this meta‐analysis suggest that while avocado intake does not significantly impact triglycerides, total cholesterol, HDL, fasting blood glucose, BMI, or CRP, it is associated with a significant reduction in LDL and systolic blood pressure. These results indicate a potential cardioprotective effect of avocado consumption by lowering key risk factors for cardiovascular diseases. However, further well‐designed studies with larger sample sizes are needed to confirm these benefits and explore the long‐term effects of avocado intake on cardiovascular health.

ObjectiveTo determine the intensity of biofilm formation on different types of central venous catheters in vitro by clinical isolates of bloodstream infection pathogens in Ukraine.MethodsFour clinical strains of Klebsiella pneumonia, four clinical strains of Staphylococcus aureus and four clinical strains of Pseudomonas aeruginosa were isolated from patients from Ukrainian tertial level children`s hospitals during 2023 with bloodstream infections including central line associated blood stream infections. Capacity to form biofilms was assessed using microtiter plate assay and ability to form biofilms in vitro was evaluated on three types of catheters: 1st catheter– surface from medical polyvinyl chloride; 2nd– surface from long-chain polymer based on methacrylate, polyethylene glycol and antiseptic polymeric biguanide; 3rd– silicon surface impregnated with an antimicrobial combination of chlorhexidine acetate and chlorhexidine. Scanning electron microscopy was conducted to assess biofilm formation on the surface of catheters.ResultsClinical isolates of K pneumonia had similar intensity of biofilm formation on different types of catheters: 1st catheter type– intensity of biofilm formation 0.30–0.34 OD; 2nd catheter type– 0.28–0.37 OD; 3rd catheter type– 0.32–0.37. Clinical isolates of S. aureus form biofilms on all types of catheters by biofilm formation on first type of catheter was lower compared to third type: 1st catheter type– 0.26–0.38 OD; 2nd catheter type– 0.3–0.4 OD; 3rd catheter type– 0.31–0.4 OD (p < 0.05 comparing with 1st catheter). Clinical isolates of P. aeruginosa had the highest ability to form biofilms on catheters. The ability to form biofilms was the most prominent of 3rd types of catheters: 1st catheter type– intensity of biofilm formation 0.38–0.66 OD; 2nd catheter type– 0.44–0.6 OD; 3rd catheter type– 0.54–0.91 OD (p < 0.05 comparing with 1st and 2nd catheter).ConclusionsP.aeruginosa clinical strains form stronger biofilms compared to other bacteria on all types of catheters. All clinical isolates were able to form biofilm on catheter after 24 h incubation however intensity of biofilm formation by S.aureus and P.aeruginosa on catheters from medical polyvinyl chloride was lower than on other types. There was no difference in biofilm formation on different types of catheters by K.pneumonia strains in vitro.

BackgroundThe increasing consumption of fast foods (FFs) and ultra-processed foods (UPFs) worldwide has raised concerns due to their association with carcinogenic compounds and potential links to various cancers. However, this evidence about breast cancer risk remains inconsistent. This study aimed to meta-analyze the association between FFs and UPFs consumption and the risk of breast cancer in females.MethodsA comprehensive search on online databases was conducted from inception to May 2025, and relevant study data were extracted. The meta-analysis utilized odds ratio (OR) with 95% confidence interval (CI) as effect size measures. Subgroup analyses, heterogeneity assessment, publication bias, and sensitivity analyses were performed to ensure robustness. All statistical analyses were conducted using STATA.ResultsThe pooled analysis of 17 observational studies showed a significant association between the highest FFs and UPFs consumption and increased breast cancer risk (OR 1.25, 95% CI [1.09–1.43], p = 0.001). Subgroup analysis revealed a significant positive association between FFs and UPFs consumption and breast cancer risk in case–control studies, but not in cohort studies or menopausal status and a significant association was observed in studies with sample sizes > 1000 and < 1000. Furthermore, the association was significant in Latin America when BMI adjustment was considered for 'yes' and 'no'.ConclusionsThis meta-analysis identified a significant association between the consumption of FFs and UPFs and an increased risk of breast cancer, with high intake linked to a 25% greater risk. These findings suggest that diets high in UPFs may play a role in breast cancer development. As UPF consumption continues to rise, public health strategies and regulatory policies targeting food processing, marketing, labeling, and accessibility are essential for cancer risk reduction and prevention.

Atrial fibrillation (AF) and stable coronary artery disease (CAD) often coexist, creating a significant burden. The efficacy of adding antiplatelet therapy to oral anticoagulant (OAC) therapy in treating these patients remains unclear, prompting this meta-analysis. A comprehensive search across databases was conducted for relevant studies. Outcomes of interest included net adverse clinical event (NACE), all-cause mortality, cardiovascular disease (CVD) mortality, major bleeding, any bleeding, hemorrhagic stroke, and ischemic stroke. A hazard ratio (HR) with 95% confidence intervals (CI) was pooled. Three randomized controlled trials (3945 patients) were analyzed. OAC monotherapy (MT) significantly reduced major bleeding (HR: 0.57; 95% CI: 0.40-0.83; P = 0.003) and any bleeding (HR: 0.55; 95% CI: 0.46-0.65; P < 0.0001) compared to combination therapy. No significant findings were observed for NACE, all-cause mortality, CVD mortality, hemorrhagic and ischemic strokes. Our meta-analysis revealed that OAC MT significantly reduced bleeding events while the number of stroke events and mortality remained similar compared to combination therapy in patients with AF and stable CAD.

The assessment of resectability in patients with colorectal cancer (CRC) and multiple liver metastases is primarily a technical decision. However, in cases with unfavourable tumor biology, the clinical benefit of surgery remains limited. Further research is needed to identify additional factors influencing oncological outcomes in these patients to better guide the decision-making process. The aim of this study was to investigatethe association of tumor burden score (TBS) and oncological outcome in CRC patients with multiple bilobar metastases. Five hundred twenty-one consecutive patients who underwent liver resections for CRC liver metastases between January 2002 and January 2024 were identified from the National Cancer Institute (Kyiv, Ukraine) prospective database and analysed retrospectively. The median and 5-year overall survival in cohorts with TBS clusters < 3, ≥ 3-9 and ≥ 9 was 116.2months, 50.3months and 29.7months; and 75.7%, 42.4% and 41.6%, respectively (P < 0.001). TBS index has shown high prognostic specificity and sensitivity for postoperative morbidity with AUC 0.97 on the ROC curve. The multivariate Cox regression model has shown that the TBS clusters have a significant negative impact on overall survival particularly in cohorts with TBS ≥ 3-9 (HR: 2.8, 95% CI: 1.7-4.8, P = 0.0001) and TBS ≥ 9 (HR: 1.4, 95% CI: 1.31-1.46, P = 0.005). In this Ukranian population-based study of patients with resectable CRC liver metastases, high TBS was associated with poorer overall survival.