Objective To enhance social connections using artificial intelligence and explore the alleviating effect of emotionally intelligent chatbots on loneliness. Method A stratified sampling method was used to distribute the Emotional Social Loneliness Inventory (ESLI) to full-time college students. Based on the ESLI assessment results, 120 young people with severe loneliness were selected as the research subjects. Regularly interact with Replika chatbot to obtain psychological support and academic assistance (continuous intervention for 1 month). Compare the scores of the ESLI scale, SASS CS scale, IES scale, and CD-RISC scale in young individuals with severe loneliness before and 1, 3, and 5 months after intervention. Results There was no statistically significant difference in ESLI scores between the two groups of college students before intervention (P>0.05). After 1, 3, and 5 months of intervention, the ESLI scores of the experimental group were lower than those of the control group (P<0.05). There was no statistically significant difference in social anxiety scores between the two groups of college students before intervention (P>0.05). After 1, 3, and 5 months of intervention, the social anxiety scores of the experimental group were lower than those of the control group (P<0.05). There was no statistically significant difference in social self-efficacy and psychological resilience levels between the two groups of college students before intervention (P>0.05). After intervention, the social self-efficacy and CD-RISC scores of the experimental group were higher than those of the control group (P<0.05). Conclusion High emotional intelligence AI chatbots can significantly improve and alleviate feelings of loneliness and enhance social skills, opening up new avenues for technology assisted psychological intervention.

Arterial hemorrhage with high-pressure, spurting blood loss can rapidly cause shock or death. Developing ideal hemostatic materials that combine rapid hemostasis, mechanical stability, antibacterial properties, and biocompatibility remains a challenge. This study reported a nanocomposite hemostatic sponge TCCND/CPL through cross-linking carboxymethyl chitosan (CMCS) and sodium carboxymethyl cellulose (CMC-Na), reinforced with nanodiamonds (NDs), and loaded with chloramphenicol (CPL) and thrombin to improve arterial hemorrhage management in irregular wounds. The incorporation of ND makes TCCND/CPL achieve a wet-state compressive stress of 44.9 kPa, which markedly exceeds typical clinical arterial systolic pressure, while maintaining elasticity, rapid absorption, and retention. Both in vitro and in vivo assessments confirmed TCCND/CPL's exceptional hemostatic capability, demonstrating superior performance to commercial agents in rat tail amputation, rat irregular trauma, femoral artery puncture, and noncompressible rabbit wound models. It achieved rapid hemostasis in just 126 s (22.7% faster than the military-grade ChitoGauze XR PRO), and the hemostatic effect was about 2.5 times that of the conventional gauze and 1.67 times that of ChitoGauze XR PRO in a rabbit femoral artery puncture model. Additionally, TCCND/CPL demonstrated significant antibacterial activity and excellent biocompatibility. The innovative design of TCCND/CPL provides an efficient solution for managing irregular wound hemorrhage in emergency scenarios such as battlefield injuries and traffic accidents.

Arene-fused o-carboranes represent a class of solid-state luminescent materials. Here we report a transition-metal-free method for constructing naphthyl- and biphenyl-fused o-carboranes via intramolecular nucleophilic substitution of the B(3)-H bond by in situ generated aryllithium intermediates under mild conditions. Single-crystal X-ray diffraction confirmed B(3)-C(aryl) bond formation, affording novel five- and six-membered fused motifs. The resulting derivatives exhibit weak or negligible emission in solutions but display strongly red-shifted and markedly enhanced fluorescence in the solid state. Naphthyl-fused species show particularly high photoluminescence efficiencies, with quantum yields up to 56%, attributable to stronger intermolecular interactions that restrict vibrational relaxation. DFT calculations reveal that C-B fusion stabilizes LUMO energies and narrows HOMO-LUMO gaps relative to C-C fused analogues, consistent with their absorption profiles. NICS analyses further establish higher aromaticity in the naphthyl-fused series. This work introduces an accessible route to π-extended carborane-arene hybrids and delineates their structure-property relationships, underscoring their potential as solid-state luminescent materials.

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) E18 (AC143, ODV-E18) is an envelope protein common to both occlusion-derived virions (ODVs) and budded virions (BVs). The e18 gene has been demonstrated to be essential for generating infectious BVs. However, its functional role in virion morphogenesis remains unclear. In this study, we constructed an e18 knockout virus and an e18 repair virus to investigate the effects of e18 deletion on virion morphogenesis. Our data indicated that e18 is required for normal intranuclear microvesicle (IMV) formation and accumulation, for intranuclear envelopment and nuclear egress of nucleocapsids, as well as for embedding of ODVs into occlusion bodies (OBs) and BV production. Additionally, we created and characterized a series of recombinant viruses with truncated e18 of varying lengths to identify domains involved in nuclear translocation and virion morphogenesis. We identified two low-complexity domains (LCDs) in E18, in addition to a known transmembrane domain (TM). The AA30-34 sequence within the TM was found to be essential, but not sufficient for nuclear translocation. However, an α-helix structure encompassing the TM domain proved adequate to mediate a fusion protein's trafficking into the nucleus in the context of additional viral factors. Furthermore, we discovered that the TM was required for the accumulation of IMVs, while both the TM and LCD 1 were necessary for intranuclear envelopment, nuclear egress of nucleocapsids, and the embedding of ODVs into OBs; LCD 2 influenced the processing of IMVs and ODV formation. Both the TM and the two LCDs were essential for BV production.IMPORTANCEThe envelope protein E18 is a conserved component common to both ODV and BV virion types of baculoviruses, yet its functional role in virion morphogenesis remains unclear. This study investigated the e18 gene of Autographa californica multiple nucleopolyhedrovirus, determining that it is essential for normal IMV formation and accumulation, intranuclear envelopment and nuclear egress of nucleocapsids, as well as for the embedding of ODVs into occlusion bodies and BV production. The functional roles of the single TM domain and two LCD domains within E18 during virion morphogenesis were identified. Furthermore, it was found that an α-helix structure encompassing the TM domain is sufficient to facilitate the trafficking of a fusion protein into the nucleus in the context of other viral factors, with AA30-34 being critical for the nuclear import of E18.