Abstract
The amounts of total phytol and its chlorophyll-bound and free portions have been determined quantitatively at different developmental stages of primary leaves of wheat using thin-layer chromatography combined with a potassium permanganate reaction. During the greening of etiolated leaves a close correlation exists between chlorophyll and phytol contents. Phytol is always present in excess and only in a bound state. During induced leaf senescence phytol is essentially degraded more slowly than chlorophyll. The liberated phytol does not influence the hydrolytic activity of chlorophyllase.
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