Abstract
In recent years, traditional Chinese medicine has played an important role in the treatment of gastric cancer in China. ZiYinHuaTan (ZYHT) recipe was developed for advanced gastric cancer and had shown its promising value in the clinic. In this study, we explore the effect of ZYHT on gastric cancer in vitro and in vivo. ZYHT can inhibit tumor growth and improve the general condition of mice in subcutaneous transplantation nude mice models of gastric cancer. And ZYHT can also inhibit cell proliferation and blocked the cells in G0/G1 to induce cell apoptosis in HGC27 and MGC803 cells. Then, network pharmacology analysis showed that ZYHT may exert antitumor effect mainly through PI3K/AKT signaling pathway. Furthermore, the expression of PI3K, p-Akt, CyclinD1, and Bcl-2 was detected in vitro and in vivo. The results showed that ZYHT could decrease the expression of PI3K, CyclinD1, and Bcl-2 both in vitro and in vivo. These results suggested that ZYHT could be used as a method for the treatment of developed gastric cancer.
Highlights
Gastric cancer (GC) is the third leading cause of cancer-related mortality in the world and the second in China [1, 2]
All decoction components (Lily, Pinellia, and Hedyotis diffusa) were purchased from Shanghai Lei Yun Shang Pharmacy Co., Ltd. (China). e extracts of ZYHT were extracted by Shanghai Changzheng Hospital Manufacturing Laboratory (Shanghai, China). e extracts of ZYHT were dissolved at a concentration of 10 mg/mL as a stock solution in RPMI-1640 medium containing 10% fetal bovine serum (FBS), followed by ultrasonic mixing overnight and filtration with a 0.22 μm filter
ZYHT therapy significantly slowed down the growth rate of tumors and reduced the tumor volume (P < 0.0) (Figure 2)
Summary
Gastric cancer (GC) is the third leading cause of cancer-related mortality in the world and the second in China [1, 2]. Surgery is currently the curative treatment for gastric cancer; chemotherapy, radiotherapy, and even gene therapy are considered the main adjuvant therapy methods. Accounting for the late detection, drug resistance, and toxic side effects, new therapies should be developed to improve the prognosis of GC. Traditional Chinese medicine (TCM) has played an important role in the treatment of gastric cancer in China. Various studies have shown that it is effective in preventing gastric cancer, reducing recurrence, alleviating pain, improving quality of life, and prolonging survival. ZiYinHuaTan (ZYHT) recipe, developed for the advanced gastric cancer, was based on the hypothesis of “tumor-phlegm microenvironment” and had shown its promising value in the clinic [3,4,5]. Several components of ZYHT have recently been reported to exert antitumor effects in several cancers. The potential role of ZYHT in the treatment of GC and the precise mechanisms have not yet been clearly addressed
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