Yüksek Yağlı Bir Diyetle Beslenen BALB/C Farelerde Kefir Tüketiminin Lipid Metabolizması ve Adipokin Hormonlar Üzerine Etkileri

Abstract

Background: The imbalance in pro-inflammatory and anti-inflammatory hormones secreted by the increase in fat tissue causes chronic inflammation in the fat tissue. It is thought that this chronic inflammation causes metabolic complications result-ing from obesity. Kefir is a type of probiotic that has recently attracted attention in the fight against obesity. This study aimed to examine the effects of kefir consumption on lipid profile and Adiponectin, Leptin, Resistin and Irisin/FNDC5 in the high fat diet fed BALB/C mouse model. Materials and Methods: BALB/C strain male mice were divided into three groups: control group (n = 10), high fat diet (HFD) (n = 10) and HFD + Kefir (n = 10). Mice were fed specific dietary patterns for eight weeks. The control group was given standard pellet feed. The HFD group was given a high-fat diet containing 52% fat. In addition to the high-fat feed, 15 ml/kg kefir was given to the HFD+Kefir group via oral gavage. Lipid profile was measured on an autoanalyzer using commercial kits. Leptin, Adiponectin, Resistin and Irisin/FNDC5 levels were measured by enzyme-linked immunosorbent assay (ELISA) using commer-cially available kits. Results: As a result of the experiment, there was no difference between the live weight gains of the groups. Epididymal fat weights in the HFD and HFD+Kefir groups were found to be statistically significantly higher than the control group. There was no significant difference between the epididymal fat weights of the HFD and HFD+Kefir groups HDL Cholesterol (HDL-C), LDL Cholesterol (LDL-C) values in the HFD and HFD+Kefir groups were found to be statistically significantly higher than the control group. No statistically significant difference was detected between the HFD+Kefir group and the HFD group in terms of HDL-C, LDL-C values. There was no difference between groups in triglyceride values. Adiponectin and Irisin/FNDC5 values of the HFD+Kefir group were found to be statistically significantly lower than the other groups. There was no statistically significant difference between the control group and the HFD group in terms of Adiponectin and Irisin/FNDC5 values. There was no significant difference between the groups in Leptin and Resistin values. Conclusions: It was thought that kefir may have metabolic effects through adipokines in the high-fat diet nutrition model, and it would be useful to support this with human studies.