Abstract
The present paper describes the first screening study of the ability of natural yeast strains to synthesize in culture the plant-related cytokine hormone zeatin, which was carried out using HPLC-MS/MS. A collection of 76 wild strains of 36 yeast species (23 genera) isolated from a variety of natural substrates was tested for the production of zeatin using HPLC-MS/MS. Zeatin was detected in more than a half (55%) of studied strains and was more frequently observed among basidiomycetous than ascomycetous species. The amount of zeatin accumulated during the experiment varied among species and strains. Highest zeatin values were recorded for basidiomycete Sporobolomyces roseus and ascomycete Taphrina sp. that produced up to 8,850.0 ng and 5,166.4 ng of zeatin per g of dry biomass, respectively. On average, the ability to produce zeatin was more pronounced among species isolated from the arctic-alpine zone than among strains from tropical and temperate climates. Our study also demonstrated that epiphytic strains and pigmented yeast species, typically for phyllosphere, are able to more often produce a plant hormone zeatin than other yeasts.
Highlights
Growth and development of plant stems, roots and fruits are largely controlled by plant growth promoters such as abscisic acid, gibberellins, auxins and cytokinins
Among studied strains highest zeatin values were observed for Sporobolomyces roseus strains KBP Y-5472 and KBP Y-5432, both isolated in the vicinity of the settlement of Dikson (Russian arctic, Kara sea) from plant surfaces
A widespread yeast species Rhodotorula mucilaginosa represented with 15 strains in this study showed the amount of zeatin ranging from 0 to 3,409.9 ng/g
Summary
Growth and development of plant stems, roots and fruits are largely controlled by plant growth promoters (plant hormones or phytohormones) such as abscisic acid, gibberellins, auxins and cytokinins. Cytokinins are organic molecules that promote plant growth through facilitated cellular division and growth. Zeatin is one of the most common cytokinins and, like other adenine-derived cytokinins, is synthesized in plant roots (Li, Li & Smith, 2017). Cytokinins produced by microorganisms have been suggested to promote the growth in higher plants (Arshad & Frankenberger, 1991; Tsavkelova et al, 2006; Arkhipova et al, 2007; Duca et al, 2014; Kudoyarova et al, 2014). Research on yeasts producing plant growth promoters in culture has been intensified in recent years.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
