XRPD lab instrument measurements and crystallographic analysis on insulin and insulin derivatives

Abstract

Introduction A successful fi rst structure refi nement of lysozyme [1] already proved the value of standard XRPD laboratory instruments for protein structure research. In this contribution we will present new measurements and crystallographic analysis results of insulin and insulin derivatives performed on data from a PANalytical X’Pert PRO diffractometer used in different confi gurations. We additionally demonstrate that even fast measurements on a 96 well plate as used for polymorph screening purposes, result in high quality data, which is suitable for automatic crystallographic analysis like indexing [2] and LeBail [3] fi tting.