Abstract
Biosensors based on the two-dimensional layered nanomaterials transition metal dichalcogenides such as WS2 and MoS2 have shown broad applications, while they largely rely on the utilization of single stranded DNA as probe biomolecules. Herein we have constructed novel WS2- and MoS2- based biosensing platforms using peptides as probe biomolecules. We have revealed for the first time that the WS2 and MoS2 nanosheets display a distinct adsorption for Arg amino acid and particularly, Arg-rich peptdies. We have demonstrated that the WS2 and MoS2 dramatically quench the fluorescence of our constructed Arg-rich probe peptide, while the hybridization of the probe peptide with its target collagen sequence leads to the fluorescence recovery. The WS2-based platform provides a sensitive fluorescence-enhanced assay that is highly specific to the target collagen peptide with little interferences from other proteins. This assay can be applied for quantitative detection of collagen biomarkers in complex biological fluids. The successful development of WS2- and MoS2- based biosensors using non-ssDNA probes opens great opportunities for the construction of novel multifunctional biosensing platforms, which may have great potential in a wide range of biomedical field.
Highlights
Graphene-based nanomaterials have shown broad biosensing applications due to their fascinating properties such as water dispersibility, biocompatibility and versatile surface modification[1,2,3,4,5,6]
Transition metal dichalcogenides nanosheets can be facilely synthesized without the need of oxidation treatment, which is a critical process for graphene oxide preparation[17,18,19]
The selective adsorption of single stranded DNA by WS2 and MoS2 nanomaterials has been proved to be a valuable strategy to construct a variety of fluorescent biosensors[20, 21, 47]
Summary
Graphene-based nanomaterials have shown broad biosensing applications due to their fascinating properties such as water dispersibility, biocompatibility and versatile surface modification[1,2,3,4,5,6]. We have further demonstrated that the coupling of the WS2 and MoS2 platforms with our constructed Arg-rich probe peptide provides a highly specific and sensitive assay for target collagen biomarkers in complex biological fluids. The fluorescent labeled peptide FAM-PRG was further investigated to develop WS2- and MoS2- based assays for the detection of target collagen peptide POG.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
