Abstract
<h3>Objectives</h3> Epithelial tissue main function is the lining of connective tissue and is characterized by juxtaposition of epithelial cells. Keratinocytes are the main cells of epithelial tissue, and they are responsible for keratin synthesis. HaCat cells are a human keratinocyte lineage widely used for in vitro studies. Withaferin A (WA) is a highly oxygenated steroidal lactone with anti-angiogenic, proapoptotic, and antiproliferative properties, and several studies demonstrated its cytotoxic effect in different types of neoplasms. This study main objective was to explore WA's effect in normal epithelial cells lineage. <h3>Study Design</h3> HaCat cells were subjected to cell viability tests (The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, Neutral Red assay, and Picogreen assay) and measurement of intracellular reactive species dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay after 24 hours of treatment in different WA concentrations. <h3>Results</h3> WA was not able to induce oxidative stress in normal keratinocyte cells and only the higher dose tested (7 µM) was a capable of decreased cell viability, but not enough to induce cell death. <h3>Conclusions</h3> Although this drug has known therapeutic potential in the treatment of different types of cancer, we can conclude that WA does not disrupt the function of normal epithelium.
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