Abstract
Resveratrol is a phytoalexin produced in a number of plant species including grapes. The benefit of resveratrol to health is widely reported. Resveratrol has been found to promote relaxation of non-pregnant and pregnant uterus, but its mechanism of action is unclear. The aims of our study were to investigate the involvement of inwardly rectifying potassium channels (Kir) in inhibitory effects of resveratrol on three models of contractions of non-pregnant rat uterus: the spontaneous rhythmic contractions (SRC), oxytocin-elicited phasic contractions and tonic oxytocin-elicited contractions.Uterine strips were obtained from virgin female Wistar rats in oestrus. Strips were mounted into organ bath for recording isometric tension in Krebs-Ringer solution. Experiments followed a multiple curve design. In order to test the involvement of Kirchannels in a mechanism of action of resveratrol(1-100 ?M),BaCl2 (1 mM),a antagonist of inwardly rectifying pota?ssium channels was used. Resveratrol induced a concentration-dependent relaxation of all models of contractions. BaCl2 antagonized the response to resveratrolon SRC and oxytocin-elicited phasic contractions. Relaxation achieved by resveratrolon tonic oxytocin-elicited concentrations was insensitive to BaCl2.The antagonism of resveratrol effects by inwardly rectifying potassium channels antagonist suggests that Kir channels are involved in resveratrol action on phasic contractions of rat uterus. Inhibitory effect of resveratrol on tonic contractions did not include Kir channels.
Highlights
Vet. glasnik 70 [3,4] 121 - 129 (2016) Radmila Novaković i sar.: Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle
That Kir channels are involved in resveratrol action on phasic contractions of rat uterus
We have shown that the ATP-sensitive potassium channels (KATP), Ca2+-sensitive big potassium channels (BKCa) and voltage-sensitive potassium channels (Kv) are involved in the inhibitory mechanism of resveratrol of the non-pregnant animal and pregnant human uterus (Novaković i sar., 2013; Novaković i sar., 2015)
Summary
The aims of our study were to investigate the involvement of inwardly rectifying potassium channels (Kir) in inhibitory effects of resveratrol on three models of contractions of non-pregnant rat uterus: the spontaneous rhythmic contractions (SRC), oxytocin-elicited phasic contractions and tonic oxytocin-elicited contractions.Uterine strips were obtained from virgin female Wistar rats in oestrus. That Kir channels are involved in resveratrol action on phasic contractions of rat uterus. (2014) have demonstrated that Kir7.1 current hyperpolarizes uterine smooth muscle cell and promotes quiescence during gestation (McCloskey i sar., 2014).The roles of the different channels are diverse and depend on the physiological environment. The aim of this study is to investigate the participation of Kir channels in inhibitory action of resveratrol on three models of uterus contractility: spontaneous rhythmic contractions (SRC), oxytocin - elicited phasic and oxytocin – elicited tonic contractions of non-pregnant rat uterus. The contractions were recording by ISOLAB software (Elunit, Belgrade, Serbia) which was connected to the transducer
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