Abstract
Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health. Since livestock products are possible reservoirs of ESBL-producing bacteria, food-borne dissemination of ESBL-producing bacteria and the characteristics of such organisms in food products should be assessed to evaluate potential sources of contamination. A total of 253 food samples from poultry, pork, shrimp, and fish were collected from local markets in a rural area of Vietnam from 2013 to 2014. ESBL-producing Escherichia coli were isolated from these samples, and their ESBL genotypes, phylogenetic groups, and antibiotic resistance profiles were assessed. Overall, a high percentage (68.4 %) of the food samples was contaminated with ESBL-producing E. coli, and samples from livestock and fishery products showed similar levels of contamination. The ESBL-producing E. coli isolated from the food samples harbored genes of the CTX-M-1, CTX-M-9, and TEM groups. Analysis of the antibiotic resistance profiles of the ESBL-producing E. coli isolates from the food samples showed a high degree of multidrug resistance. The prevalence of extensively multidrug-resistant ESBL-producing E. coli that were resistant to at least five antibiotic drug classes in poultry, pork, shrimp, and fish samples, was 92.1, 69, 56.5, and 62.5 %, respectively. The results of this study confirmed the widespread dissemination of ESBL-producing E. coli in both livestock and fishery products from a rural area in Vietnam. The high prevalence of extensively multidrug-resistant ESBL-producing E. coli in food products highlights the importance of continuous monitoring of food products for the presence of these bacteria, particularly in underdeveloped countries.
Highlights
Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health
The Polymerase chain reaction (PCR) conditions used were as follows: initial denaturation at 95 °C for 5 min, followed by 25 cycles of denaturation at 95 °C for 30 s, annealing at 60 °C for 90 s, and extension at 72 °C for 90 s, with a final extension at 68 °C for Prevalence of ESBL-E. coli in food samples A high percentage (68.4 %) of the tested poultry, pork, shrimp, and fish samples was contaminated with ESBL-E. coli (Table 1)
Genotypes of ESBL-E. coli Genotype analysis showed that the majority of the ESBL-E. coli isolates belonged to the CTX-M group (359/372 [96.5 %])
Summary
Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health. Since livestock products are possible reservoirs of ESBL-producing bacteria, food-borne dissemination of ESBL-producing bacteria and the characteristics of such organisms in food products should be assessed to evaluate potential sources of contamination. Antimicrobial drug resistance is a growing public health concern worldwide (Hawkey and Jones 2009), and in particular, the increasing prevalence of extendedspectrum β-lactamase (ESBL)-producing bacteria is an emerging threat to public health (Pitout and Laupland 2008; Woerther et al 2013). Limited studies on the prevalence of ESBL-producing bacteria have been conducted in Asian countries, the results show widespread dissemination of these bacteria in the community (Sasaki et al 2010; Nakayama et al 2015).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
