Abstract
Clostridium butyricum is a strictly anaerobic, butyric acid-producing, Gram-positive, spore-forming bacillus that is commonly present in the gut of humans. The complete genome sequence of Clostridium butyricum UBCB 70 was studied to evaluate the presence of antibiotic-resistant and clostridium toxin genes. Here, we announce the draft genome sequence of Clostridium butyricum UBCB 70, isolated from healthy human feces at Unique Biotech Limited, Hyderabad, Telangana, India.
Highlights
Clostridium butyricum is a strictly anaerobic, butyric acid-producing, Grampositive, spore-forming bacillus that is commonly present in the gut of humans
A total of 17,652,182 paired-end reads of 150-bp read length on average with a genome coverage of 1,171ϫ were sequenced, out of which 12,864,290 high-quality paired-end reads were filtered with the NGS QC Toolkit (5)
The high-quality paired-end reads were assembled into 81 contigs with the de novo genome assembler SPAdes version 3.11.1 (6) and the scaffolder SSPACE-standard version 3.0 (7)
Summary
Clostridium butyricum is a strictly anaerobic, butyric acid-producing, Grampositive, spore-forming bacillus that is commonly present in the gut of humans. The genomic DNA from the culture pellet was fragmented with an ultrasonicator to generate 300 to 400-bp fragments. 100 ng of fragmented DNA was used to prepare a paired-end sequencing library with a NEBNext UltraTMII DNA library prep kit, and sequencing was performed on an Illumina HiSeq platform.
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