Abstract
BackgroundMultidrug-resistant Salmonella is an important cause of morbidity and mortality in developing countries. The aim of this study was to characterize and compare multidrug-resistant Salmonella enterica serovar Typhimurium isolates from patients and poultry feces.MethodsSalmonella strains were isolated from poultry and patients using standard bacteriological methods described in previous studies. The strains were serotype according to Kaufmann-White scheme and tested for antibiotic susceptibility to 12 different antimicrobial agents using the disk diffusion method. The whole genome of the S. Typhimurium isolates was analyzed using Illumina technology and compared with 20 isolates of S. Typhimurium for which the ST has been deposited in a global MLST database.The ResFinder Web server was used to find the antibiotic resistance genes from whole genome sequencing (WGS) data. For comparative genomics, publicly available complete and draft genomes of different S. Typhimurium laboratory-adapted strains were downloaded from GenBank.ResultsAll the tested Salmonella serotype Typhimurium were multiresistant to five commonly used antibiotics (ampicillin, chloramphenicol, streptomycin, sulfonamide, and trimethoprim). The multilocus sequence type ST313 was detected from all the strains. Our sequences were very similar to S. Typhimurium ST313 strain D23580 isolated from a patient with invasive non-typhoid Salmonella (NTS) infection in Malawi, also located in sub-Saharan Africa. The use of ResFinder web server on the whole genome of the strains showed a resistance to aminoglycoside associated with carriage of the following resistances genes: strA, strB, and aadA1; resistance to β-lactams associated with carriage of a blaTEM-1B genes; resistance to phenicol associated with carriage of catA1 gene; resistance to sulfonamide associated with carriage of sul1 and sul2 genes; resistance to tetracycline associated with carriage of tet B gene; and resistance to trimethoprim associated to dfrA1 gene for all the isolates.ConclusionThe poultry and human isolates were genetically similar showing a potential food safety risk for consumers. Our finding of multidrug-resistant S. Typhimurium ST313 in poultry feces calls for further studies to clarify the potential reservoirs of this emerging pathogen.
Highlights
Multidrug-resistant Salmonella is an important cause of morbidity and mortality in developing countries
Typhimurium strains isolated from the feces of humans and poultry in Burkina Faso using whole genome sequencing (WGS) and to compare the sequences to each other as well as to other previously published genomes of MDR S
The multilocus sequence typing (MLST) analysis of the WGS data revealed that all the isolates were of the same sequence type, ST313
Summary
Multidrug-resistant Salmonella is an important cause of morbidity and mortality in developing countries. The global burden of diarrheal disease caused by Salmonella gastroenteric infections is substantial, with estimated 93.8 million human cases per year and 155,000 deaths [1]. These diseases are frequent among children in developing countries such as Burkina Faso, where they often go unreported because of the lack of the foodborne pathogens surveillance system. In Burkina Faso, malaria combined with invasive bacterial infection caused by antibiotic-resistant Salmonella has been found to be related to high case fatality rate among children [4]. The source of the invasive infections and the possible role of animals spreading the pathogen are not well known
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
