Abstract
ABSTRACTWe report here the complete genome sequence of Mycobacterium dioxanotrophicus PH-06, which is capable of using 1,4-dioxane as a sole source of carbon and energy. The reported sequence will enable the elucidation of this novel metabolic pathway and the development of molecular biomarkers to assess bioremediation potential at contaminated sites.
Highlights
We report here the complete genome sequence of Mycobacterium dioxanotrophicus PH-06, which is capable of using 1,4-dioxane as a sole source of carbon and energy
PH-06 was grown in ammonium mineral salts medium [5] amended with 500 mg/L of dioxane and incubated at 30°C while shaking at 150 rpm
Cells were harvested during exponential growth, and genomic DNA was extracted using the UltraClean microbial DNA isolation kit (Mo Bio, Inc.)
Summary
We report here the complete genome sequence of Mycobacterium dioxanotrophicus PH-06, which is capable of using 1,4-dioxane as a sole source of carbon and energy. Cells were harvested during exponential growth, and genomic DNA was extracted using the UltraClean microbial DNA isolation kit (Mo Bio, Inc.). Whole-genome sequencing was conducted using both the PacBio RS II (Yale Center for Genome Analysis, http://ycga.yale.edu) and Illumina HiSeq 4000 (Beijing Genomic Institute [BGI], http://www.genomics.cn) platforms. The whole genome was assembled and annotated in collaboration with BGI as follows.
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