What Quantity of Photosystem I Is Optimum for Safe Photosynthesis?

Abstract

PSI has the potential to generate reactive oxygen species and be oxidatively inactivated by the reactive oxygen species. The photo-oxidative damage of PSI (also called PSI photoinhibition) causes the inhibition of the plant growth and is a lethal event for plants. It has been reported that PSI photoinhibition does not occur as long as the reaction-center chlorophyll (P700) remains oxidized, even in excess light conditions. This process is termed P700 oxidation and is supported by various regulatory mechanisms and likely also by the stoichiometric quantities of photosynthetic apparatus. In this study, we assessed how decreased photochemically active PSI in Arabidopsis (Arabidopsis thaliana) affected a variety of photosynthetic parameters, including P700 oxidation. Inactivation of PSI was rapidly and selectively induced by repetitive short-pulse illumination. PSI photoinhibition correlated linearly with decreases in effective quantum yield of PSII and nonphotochemical quenching; however, the photosynthetic CO2 assimilation rate was less affected, as exemplified by ∼50% of the normal CO2 assimilation rate maintained with an 80% loss in PSI photochemical activity. In contrast, effective quantum yield of PSI was enhanced following PSI photoinhibition, mainly owing to a decrease in the electron donor-side limitation of PSI. Based on these results, we propose that the stoichiometric quantity of PSI is optimized to induce P700 oxidation for dissipating excess light energy in PSI, thus avoiding inhibition of photosynthetic CO2 assimilation caused by PSI photoinhibition.