Abstract
West Nile Virus, Venezuela
Highlights
The flavivirus generating the immunoglobulin G (IgG) response was identified by using the following criteria: 90% inhibition of virus in serum diluted at least 1:40 and 4-fold greater neutralizing antibody titer compared with closely related flaviviruses
One serum sample cross-reacted with other flaviviruses tested, with equivalent titers to West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), and Ilheus virus (ILHV) and was considered infected with an undetermined flavivirus
Antibody against flavivirus was detected by ELISA in 141 of 791 horses, and 34 (4.3%) were confirmed positive for WNV infection by plaque reduction neutralization test (PRNT); viral titers ≥640 occurred in half of these horses
Summary
Infected horses and birds have been reported in various Caribbean Islands, Mexico, and northern Central America (2,3). Serum samples from birds and horses from 33 locations in Venezuela (Online Appendix Table, available from http://www.cdc.gov/ E I D / content / 1 3 / 4 / 6 5 1 - a p p T. h t m ) were screened for immunoglobulin G (IgG) antibodies against WNV antigen by ELISA (6) and confirmed by plaque reduction neutralization test (PRNT) as previously described (7). The flavivirus generating the IgG response was identified by using the following criteria: 90% inhibition of virus in serum diluted at least 1:40 and 4-fold greater neutralizing antibody titer compared with closely related flaviviruses.
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